3ktt - Revision history

OCA at 10:18, 21 February 2024

2024-02-21T10:18:24Z

←Older revision		Revision as of 10:18, 21 February 2024
Line 3:		Line 3:
	<SX load='3ktt' size='340' side='right' viewer='molstar' caption='[[3ktt]], [[Resolution\|resolution]] 4.00Å' scene=''>		<SX load='3ktt' size='340' side='right' viewer='molstar' caption='[[3ktt]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ktt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3KTT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ktt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KTT FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[[~~3iyg~~\|~~3iyg~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3ktt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktt OCA], [~~http~~://pdbe.org/3ktt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ktt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ktt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ktt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ktt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktt OCA], [https://pdbe.org/3ktt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ktt RCSB], [https://www.ebi.ac.uk/pdbsum/3ktt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ktt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TCPB_BOVIN TCPB_BOVIN]] Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity).	+	[https://www.uniprot.org/uniprot/TCPB_BOVIN TCPB_BOVIN] Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ktt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ktt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The essential double-ring eukaryotic chaperonin TRiC/CCT (TCP1-ring complex or chaperonin containing TCP1) assists the folding of approximately 5-10% of the cellular proteome. Many TRiC substrates cannot be folded by other chaperonins from prokaryotes or archaea. These unique folding properties are likely linked to TRiC's unique heterooligomeric subunit organization, whereby each ring consists of eight different paralogous subunits in an arrangement that remains uncertain. Using single particle cryo-EM without imposing symmetry, we determined the mammalian TRiC structure at 4.7-A resolution. This revealed the existence of a 2-fold axis between its two rings resulting in two homotypic subunit interactions across the rings. A subsequent 2-fold symmetrized map yielded a 4.0-A resolution structure that evinces the densities of a large fraction of side chains, loops, and insertions. These features permitted unambiguous identification of all eight individual subunits, despite their sequence similarity. Independent biochemical near-neighbor analysis supports our cryo-EM derived TRiC subunit arrangement. We obtained a Calpha backbone model for each subunit from an initial homology model refined against the cryo-EM density. A subsequently optimized atomic model for a subunit showed approximately 95% of the main chain dihedral angles in the allowable regions of the Ramachandran plot. The determination of the TRiC subunit arrangement opens the way to understand its unique function and mechanism. In particular, an unevenly distributed positively charged wall lining the closed folding chamber of TRiC differs strikingly from that of prokaryotic and archaeal chaperonins. These interior surface chemical properties likely play an important role in TRiC's cellular substrate specificity.
-
-	4.0-A resolution cryo-EM structure of the mammalian chaperonin TRiC/CCT reveals its unique subunit arrangement.,Cong Y, Baker ML, Jakana J, Woolford D, Miller EJ, Reissmann S, Kumar RN, Redding-Johanson AM, Batth TS, Mukhopadhyay A, Ludtke SJ, Frydman J, Chiu W Proc Natl Acad Sci U S A. 2010 Mar 1. PMID:20194787<ref>PMID:20194787</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3ktt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Chaperonin 3D structures\|Chaperonin 3D structures]]		*[[Chaperonin 3D structures\|Chaperonin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baker~~, M L~~]]	+	[[Category: Baker ML]]
-	[[Category: Chiu, W]]	+	[[Category: Chiu W]]
-	[[Category: Cong, Y]]	+	[[Category: Cong Y]]
-	[[Category: Frydman, J]]	+	[[Category: Frydman J]]
-	[[Category: Ludtke~~, S J]]~~	+	[[Category: Ludtke SJ]]
-	~~[[Category: Acetylation]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Cryo-em]]~~	+
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Tric/cct~~]]	+

OCA at 18:39, 10 April 2020

2020-04-10T18:39:35Z

←Older revision		Revision as of 18:39, 10 April 2020
Line 3:		Line 3:
	<SX load='3ktt' size='340' side='right' viewer='molstar' caption='[[3ktt]], [[Resolution\|resolution]] 4.00Å' scene=''>		<SX load='3ktt' size='340' side='right' viewer='molstar' caption='[[3ktt]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ktt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3KTT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ktt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3KTT FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3iyg\|3iyg]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3iyg\|3iyg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ktt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktt OCA], [http://pdbe.org/3ktt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ktt RCSB], [http://www.ebi.ac.uk/pdbsum/3ktt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ktt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ktt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktt OCA], [http://pdbe.org/3ktt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ktt RCSB], [http://www.ebi.ac.uk/pdbsum/3ktt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ktt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:43, 6 March 2020

2020-03-06T17:43:45Z

←Older revision		Revision as of 17:43, 6 March 2020
Line 1:		Line 1:

	==Atomic model of bovine TRiC CCT2(beta) subunit derived from a 4.0 Angstrom cryo-EM map==		==Atomic model of bovine TRiC CCT2(beta) subunit derived from a 4.0 Angstrom cryo-EM map==
-	<~~StructureSection~~ load='3ktt' size='340' side='right' caption='[[3ktt]], [[Resolution\|resolution]] 4.00Å' scene=''>	+	<SX load='3ktt' size='340' side='right' viewer='molstar' caption='[[3ktt]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ktt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KTT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ktt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KTT FirstGlance]. <br>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Chaperonin\|Chaperonin]]	+	*[[Chaperonin 3D structures\|Chaperonin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
	[[Category: Baker, M L]]		[[Category: Baker, M L]]
	[[Category: Chiu, W]]		[[Category: Chiu, W]]

OCA at 08:32, 18 July 2018

2018-07-18T08:32:10Z

←Older revision		Revision as of 08:32, 18 July 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kt/3ktt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kt/3ktt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 41:		Line 41:
	[[Category: Frydman, J]]		[[Category: Frydman, J]]
	[[Category: Ludtke, S J]]		[[Category: Ludtke, S J]]
		+	[[Category: Acetylation]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
	[[Category: Cryo-em]]		[[Category: Cryo-em]]
		+	[[Category: Cytoplasm]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
	[[Category: Tric/cct]]		[[Category: Tric/cct]]

OCA at 09:08, 4 August 2016

2016-08-04T09:08:26Z

←Older revision		Revision as of 09:08, 4 August 2016
Line 1:		Line 1:
		+
	==Atomic model of bovine TRiC CCT2(beta) subunit derived from a 4.0 Angstrom cryo-EM map==		==Atomic model of bovine TRiC CCT2(beta) subunit derived from a 4.0 Angstrom cryo-EM map==
	<StructureSection load='3ktt' size='340' side='right' caption='[[3ktt]], [[Resolution\|resolution]] 4.00Å' scene=''>		<StructureSection load='3ktt' size='340' side='right' caption='[[3ktt]], [[Resolution\|resolution]] 4.00Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3ktt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KTT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ktt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KTT FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3iyg\|3iyg]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3iyg\|3iyg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ktt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ktt RCSB], [http://www.ebi.ac.uk/pdbsum/3ktt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ktt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktt OCA], [http://pdbe.org/3ktt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ktt RCSB], [http://www.ebi.ac.uk/pdbsum/3ktt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ktt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ktt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ktt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 08:02, 24 December 2014

2014-12-24T08:02:29Z

←Older revision		Revision as of 08:02, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ktt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ktt RCSB], [http://www.ebi.ac.uk/pdbsum/3ktt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ktt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ktt RCSB], [http://www.ebi.ac.uk/pdbsum/3ktt PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TCPB_BOVIN TCPB_BOVIN]] Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:27, 9 December 2014

2014-12-09T09:27:14Z

←Older revision		Revision as of 09:27, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3ktt.~~png~~\|~~left~~\|200px]]	+	==Atomic model of bovine TRiC CCT2(beta) subunit derived from a 4.0 Angstrom cryo-EM map==
		+	<StructureSection load='3ktt' size='340' side='right' caption='[[3ktt]], [[Resolution\|resolution]] 4.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ktt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KTT FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3iyg\|3iyg]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ktt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ktt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ktt RCSB], [http://www.ebi.ac.uk/pdbsum/3ktt PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kt/3ktt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The essential double-ring eukaryotic chaperonin TRiC/CCT (TCP1-ring complex or chaperonin containing TCP1) assists the folding of approximately 5-10% of the cellular proteome. Many TRiC substrates cannot be folded by other chaperonins from prokaryotes or archaea. These unique folding properties are likely linked to TRiC's unique heterooligomeric subunit organization, whereby each ring consists of eight different paralogous subunits in an arrangement that remains uncertain. Using single particle cryo-EM without imposing symmetry, we determined the mammalian TRiC structure at 4.7-A resolution. This revealed the existence of a 2-fold axis between its two rings resulting in two homotypic subunit interactions across the rings. A subsequent 2-fold symmetrized map yielded a 4.0-A resolution structure that evinces the densities of a large fraction of side chains, loops, and insertions. These features permitted unambiguous identification of all eight individual subunits, despite their sequence similarity. Independent biochemical near-neighbor analysis supports our cryo-EM derived TRiC subunit arrangement. We obtained a Calpha backbone model for each subunit from an initial homology model refined against the cryo-EM density. A subsequently optimized atomic model for a subunit showed approximately 95% of the main chain dihedral angles in the allowable regions of the Ramachandran plot. The determination of the TRiC subunit arrangement opens the way to understand its unique function and mechanism. In particular, an unevenly distributed positively charged wall lining the closed folding chamber of TRiC differs strikingly from that of prokaryotic and archaeal chaperonins. These interior surface chemical properties likely play an important role in TRiC's cellular substrate specificity.

-	~~{{STRUCTURE_3ktt\| PDB=3ktt \| SCENE= }}~~	+	4.0-A resolution cryo-EM structure of the mammalian chaperonin TRiC/CCT reveals its unique subunit arrangement.,Cong Y, Baker ML, Jakana J, Woolford D, Miller EJ, Reissmann S, Kumar RN, Redding-Johanson AM, Batth TS, Mukhopadhyay A, Ludtke SJ, Frydman J, Chiu W Proc Natl Acad Sci U S A. 2010 Mar 1. PMID:20194787<ref>PMID:20194787</ref>

-	~~===Atomic model~~ of ~~bovine TRiC CCT2(beta) subunit derived from a 4~~.~~0 Angstrom cryo-EM map===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20194787}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ktt]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA].~~	+

	==See Also==		==See Also==
	*[[Chaperonin\|Chaperonin]]		*[[Chaperonin\|Chaperonin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020194787<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
-	[[Category: Baker, M L.]]	+	[[Category: Baker, M L]]
-	[[Category: Chiu, W.]]	+	[[Category: Chiu, W]]
-	[[Category: Cong, Y.]]	+	[[Category: Cong, Y]]
-	[[Category: Frydman, J.]]	+	[[Category: Frydman, J]]
-	[[Category: Ludtke, S J.]]	+	[[Category: Ludtke, S J]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Chaperone]]		[[Category: Chaperone]]

OCA at 05:38, 20 October 2012

2012-10-20T05:38:31Z

←Older revision		Revision as of 05:38, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3ktt]] is a 1 chain structure ~~of [[Chaperonin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA].	+	[[3ktt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA].

	==See Also==		==See Also==

OCA: Protected "3ktt" [edit=sysop:move=sysop]

2012-07-25T10:42:19Z

Protected "3ktt" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:42, 25 July 2012

OCA at 10:42, 25 July 2012

2012-07-25T10:42:17Z

←Older revision		Revision as of 10:42, 25 July 2012
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-	{{Seed}}
	[[Image:3ktt.png\|left\|200px]]		[[Image:3ktt.png\|left\|200px]]

-	<!--
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_3ktt\| PDB=3ktt \| SCENE= }}		{{STRUCTURE_3ktt\| PDB=3ktt \| SCENE= }}

	===Atomic model of bovine TRiC CCT2(beta) subunit derived from a 4.0 Angstrom cryo-EM map===		===Atomic model of bovine TRiC CCT2(beta) subunit derived from a 4.0 Angstrom cryo-EM map===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20194787}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20194787 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_20194787}}		{{ABSTRACT_PUBMED_20194787}}

	==About this Structure==		==About this Structure==
-	~~3KTT~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA].	+	[[3ktt]] is a 1 chain structure of [[Chaperonin]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KTT OCA].
		+
		+	==See Also==
		+	*[[Chaperonin\|Chaperonin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20194787~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020194787</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Baker, M L.]]		[[Category: Baker, M L.]]
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	[[Category: Frydman, J.]]		[[Category: Frydman, J.]]
	[[Category: Ludtke, S J.]]		[[Category: Ludtke, S J.]]
-	[[Category: Acetylation]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
	[[Category: Cryo-em]]		[[Category: Cryo-em]]
-	[[Category: Cytoplasm]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
	[[Category: Tric/cct]]		[[Category: Tric/cct]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 7 10:02:54 2010''