3kvu - Revision history

OCA at 08:25, 6 September 2023

2023-09-06T08:25:19Z

←Older revision		Revision as of 08:25, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3kvu' size='340' side='right'caption='[[3kvu]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3kvu' size='340' side='right'caption='[[3kvu]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kvu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces~~ cattleya~~" kahan et al. 1979~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kvu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KVU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3kuv\|3kuv]], [[3kuw\|3kuw]], [[3kv7\|3kv7]], [[3kv8\|3kv8]], [[3kvi\|3kvi]], [[3kvz\|3kvz]], [[3kw1\|3kw1]], [[3kx7\|3kx7]], [[3kx8\|3kx8]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">flK, fluoroacetyl-CoA thioesterase FlK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvu OCA], [https://pdbe.org/3kvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kvu RCSB], [https://www.ebi.ac.uk/pdbsum/3kvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kvu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvu OCA], [https://pdbe.org/3kvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kvu RCSB], [https://www.ebi.ac.uk/pdbsum/3kvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kvu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>	+	[https://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces cattleya kahan et al. 1979]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Blundell~~, T L~~]]	+	[[Category: Streptomyces cattleya]]
-	[[Category: Chirgadze~~, D Y~~]]	+	[[Category: Blundell TL]]
-	[[Category: Dias~~, M V.B~~]]	+	[[Category: Chirgadze DY]]
-	[[Category: Huang, F]]	+	[[Category: Dias MVB]]
-	[[Category: Leadlay~~, P F~~]]	+	[[Category: Huang F]]
-	[[Category: Spencer~~, J B~~]]	+	[[Category: Leadlay PF]]
-	[[Category: Spiteller, D]]	+	[[Category: Spencer JB]]
-	[[Category: Tosin, M]]	+	[[Category: Spiteller D]]
-	[[Category: Valentine~~, E F]]~~	+	[[Category: Tosin M]]
-	~~[[Category: Fluoroacetyl-coa thioesterase flk]]~~	+	[[Category: Valentine EF]]
-	~~[[Category: Hot-dog folding]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Thioesterase~~]]	+

OCA at 12:29, 13 October 2021

2021-10-13T12:29:29Z

←Older revision		Revision as of 12:29, 13 October 2021
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	==Structural basis of the activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA==		==Structural basis of the activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA==
-	<StructureSection load='3kvu' size='340' side='right' caption='[[3kvu]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3kvu' size='340' side='right'caption='[[3kvu]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kvu]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces cattleya" kahan et al. 1979]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3KVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kvu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces cattleya" kahan et al. 1979]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KVU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kuv\|3kuv]], [[3kuw\|3kuw]], [[3kv7\|3kv7]], [[3kv8\|3kv8]], [[3kvi\|3kvi]], [[3kvz\|3kvz]], [[3kw1\|3kw1]], [[3kx7\|3kx7]], [[3kx8\|3kx8]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3kuv\|3kuv]], [[3kuw\|3kuw]], [[3kv7\|3kv7]], [[3kv8\|3kv8]], [[3kvi\|3kvi]], [[3kvz\|3kvz]], [[3kw1\|3kw1]], [[3kx7\|3kx7]], [[3kx8\|3kx8]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK, fluoroacetyl-CoA thioesterase FlK ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK, fluoroacetyl-CoA thioesterase FlK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3kvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvu OCA], [~~http~~://pdbe.org/3kvu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3kvu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3kvu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3kvu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvu OCA], [https://pdbe.org/3kvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kvu RCSB], [https://www.ebi.ac.uk/pdbsum/3kvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kvu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>	+	[[https://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 3kvu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3kvu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thioesterase 3D structures\|Thioesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces cattleya kahan et al. 1979]]		[[Category: Streptomyces cattleya kahan et al. 1979]]
		+	[[Category: Large Structures]]
	[[Category: Blundell, T L]]		[[Category: Blundell, T L]]
	[[Category: Chirgadze, D Y]]		[[Category: Chirgadze, D Y]]

OCA at 19:24, 24 January 2018

2018-01-24T19:24:00Z

←Older revision		Revision as of 19:24, 24 January 2018
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kv/3kvu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kv/3kvu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 3kvu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3kvu" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Thioesterase\|Thioesterase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:52, 5 August 2016

2016-08-05T15:52:26Z

←Older revision		Revision as of 15:52, 5 August 2016
Line 1:		Line 1:
		+
	==Structural basis of the activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA==		==Structural basis of the activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA==
	<StructureSection load='3kvu' size='340' side='right' caption='[[3kvu]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3kvu' size='340' side='right' caption='[[3kvu]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_cattleya Streptomyces~~ cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces cattleya" kahan et al. 1979]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KVU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kuv\|3kuv]], [[3kuw\|3kuw]], [[3kv7\|3kv7]], [[3kv8\|3kv8]], [[3kvi\|3kvi]], [[3kvz\|3kvz]], [[3kw1\|3kw1]], [[3kx7\|3kx7]], [[3kx8\|3kx8]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kuv\|3kuv]], [[3kuw\|3kuw]], [[3kv7\|3kv7]], [[3kv8\|3kv8]], [[3kvi\|3kvi]], [[3kvz\|3kvz]], [[3kw1\|3kw1]], [[3kx7\|3kx7]], [[3kx8\|3kx8]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK, fluoroacetyl-CoA thioesterase FlK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 Streptomyces cattleya])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK, fluoroacetyl-CoA thioesterase FlK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kvu RCSB], [http://www.ebi.ac.uk/pdbsum/3kvu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvu OCA], [http://pdbe.org/3kvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kvu RCSB], [http://www.ebi.ac.uk/pdbsum/3kvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kvu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q1EMV2_STRCT Q1EMV2_STRCT~~]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>	+	[[http://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kvu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3kvu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces cattleya]]	+	[[Category: Streptomyces cattleya kahan et al. 1979]]
	[[Category: Blundell, T L]]		[[Category: Blundell, T L]]
	[[Category: Chirgadze, D Y]]		[[Category: Chirgadze, D Y]]

OCA at 03:45, 25 December 2014

2014-12-25T03:45:27Z

←Older revision		Revision as of 03:45, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kvu RCSB], [http://www.ebi.ac.uk/pdbsum/3kvu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kvu RCSB], [http://www.ebi.ac.uk/pdbsum/3kvu PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q1EMV2_STRCT Q1EMV2_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:48, 9 December 2014

2014-12-09T08:48:50Z

←Older revision		Revision as of 08:48, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3kvu.~~png~~\|~~left~~\|200px]]	+	==Structural basis of the activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA==
		+	<StructureSection load='3kvu' size='340' side='right' caption='[[3kvu]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3kvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KVU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kuv\|3kuv]], [[3kuw\|3kuw]], [[3kv7\|3kv7]], [[3kv8\|3kv8]], [[3kvi\|3kvi]], [[3kvz\|3kvz]], [[3kw1\|3kw1]], [[3kx7\|3kx7]], [[3kx8\|3kx8]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK, fluoroacetyl-CoA thioesterase FlK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 Streptomyces cattleya])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kvu RCSB], [http://www.ebi.ac.uk/pdbsum/3kvu PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kv/3kvu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The thioesterase FlK from the fluoroacetate-producing Streptomyces cattleya catalyzes the hydrolysis of fluoroacetyl-coenzyme A. This provides an effective self-defense mechanism, preventing any fluoroacetyl-coenzyme A formed from being further metabolized to 4-hydroxy-trans-aconitate, a lethal inhibitor of the tricarboxylic acid cycle. Remarkably, FlK does not accept acetyl-coenzyme A as a substrate. Crystal structure analysis shows that FlK forms a dimer, in which each subunit adopts a hot dog fold as observed for type II thioesterases. Unlike other type II thioesterases, which invariably utilize either an aspartate or a glutamate as catalytic base, we show by site-directed mutagenesis and crystallography that FlK employs a catalytic triad composed of Thr(42), His(76), and a water molecule, analogous to the Ser/Cys-His-acid triad of type I thioesterases. Structural comparison of FlK complexed with various substrate analogues suggests that the interaction between the fluorine of the substrate and the side chain of Arg(120) located opposite to the catalytic triad is essential for correct coordination of the substrate at the active site and therefore accounts for the substrate specificity.

-	~~{{STRUCTURE_3kvu\| PDB=3kvu \| SCENE= }}~~	+	Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK.,Dias MV, Huang F, Chirgadze DY, Tosin M, Spiteller D, Dry EF, Leadlay PF, Spencer JB, Blundell TL J Biol Chem. 2010 Jul 16;285(29):22495-504. Epub 2010 Apr 29. PMID:20430898<ref>PMID:20430898</ref>

-	~~===Structural basis~~ of the ~~activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20430898}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3kvu]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_cattleya Streptomyces cattleya]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA].~~	+

	==See Also==		==See Also==
	*[[Thioesterase\|Thioesterase]]		*[[Thioesterase\|Thioesterase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020430898<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces cattleya]]		[[Category: Streptomyces cattleya]]
-	[[Category: Blundell, T L.]]	+	[[Category: Blundell, T L]]
-	[[Category: Chirgadze, D Y.]]	+	[[Category: Chirgadze, D Y]]
-	[[Category: Dias, M V.B.]]	+	[[Category: Dias, M V.B]]
-	[[Category: Huang, F.]]	+	[[Category: Huang, F]]
-	[[Category: Leadlay, P F.]]	+	[[Category: Leadlay, P F]]
-	[[Category: Spencer, J B.]]	+	[[Category: Spencer, J B]]
	[[Category: Spiteller, D]]		[[Category: Spiteller, D]]
-	[[Category: Tosin, M.]]	+	[[Category: Tosin, M]]
-	[[Category: Valentine, E F.]]	+	[[Category: Valentine, E F]]
	[[Category: Fluoroacetyl-coa thioesterase flk]]		[[Category: Fluoroacetyl-coa thioesterase flk]]
	[[Category: Hot-dog folding]]		[[Category: Hot-dog folding]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Thioesterase]]		[[Category: Thioesterase]]

OCA at 12:18, 20 October 2012

2012-10-20T12:18:52Z

←Older revision		Revision as of 12:18, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3kvu]] is a 4 chain structure ~~of [[Thioesterase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA].	+	[[3kvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA].

	==See Also==		==See Also==

OCA: Protected "3kvu" [edit=sysop:move=sysop]

2012-07-26T08:19:35Z

Protected "3kvu" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:19, 26 July 2012

OCA at 08:19, 26 July 2012

2012-07-26T08:19:34Z

←Older revision		Revision as of 08:19, 26 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3kvu.png\|left\|200px]]		[[Image:3kvu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3kvu", creates the "Structure Box" on the page.
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-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_3kvu\| PDB=3kvu \| SCENE= }}		{{STRUCTURE_3kvu\| PDB=3kvu \| SCENE= }}

	===Structural basis of the activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA===		===Structural basis of the activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20430898}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20430898 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_20430898}}		{{ABSTRACT_PUBMED_20430898}}

	==About this Structure==		==About this Structure==
-	~~3KVU~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA].	+	[[3kvu]] is a 4 chain structure of [[Thioesterase]] with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA].
		+
		+	==See Also==
		+	*[[Thioesterase\|Thioesterase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20430898~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020430898</ref><references group="xtra"/>
	[[Category: Streptomyces cattleya]]		[[Category: Streptomyces cattleya]]
	[[Category: Blundell, T L.]]		[[Category: Blundell, T L.]]
Line 38:		Line 29:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Thioesterase]]		[[Category: Thioesterase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 30 13:21:06 2010''

OCA at 09:15, 30 June 2010

2010-06-30T09:15:30Z

←Older revision		Revision as of 09:15, 30 June 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3kvu.~~jpg~~\|left\|200px]]	+	[[Image:3kvu.png\|left\|200px]]

	<!--		<!--
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	===Structural basis of the activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA===		===Structural basis of the activity and substrate specificity of the fluoroacetyl-CoA FlK - T42S mutant in complex with Acetyl-CoA===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20430898}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20430898 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20430898}}

	==About this Structure==		==About this Structure==
	3KVU is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA].		3KVU is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KVU OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20430898</ref><references group="xtra"/>
	[[Category: Streptomyces cattleya]]		[[Category: Streptomyces cattleya]]
	[[Category: Blundell, T L.]]		[[Category: Blundell, T L.]]
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	[[Category: Thioesterase]]		[[Category: Thioesterase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Apr 28 11~~:06:16 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 30 13:21:06 2010''