3l2a - Revision history

OCA at 08:28, 6 September 2023

2023-09-06T08:28:55Z

←Older revision		Revision as of 08:28, 6 September 2023
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	<StructureSection load='3l2a' size='340' side='right'caption='[[3l2a]], [[Resolution\|resolution]] 1.71Å' scene=''>		<StructureSection load='3l2a' size='340' side='right'caption='[[3l2a]], [[Resolution\|resolution]] 1.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3l2a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Eborr Eborr~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L2A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3l2a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Reston_ebolavirus_-_Reston_(1989) Reston ebolavirus - Reston (1989)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L2A FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.71Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l27\|3l27]], [[3l28\|3l28]], [[3l29\|3l29]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">REBOVgp2, VP35 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=386032 EBORR])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2a OCA], [https://pdbe.org/3l2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l2a RCSB], [https://www.ebi.ac.uk/pdbsum/3l2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l2a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2a OCA], [https://pdbe.org/3l2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l2a RCSB], [https://www.ebi.ac.uk/pdbsum/3l2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l2a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/VP35_EBORR VP35_EBORR]] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).	+	[https://www.uniprot.org/uniprot/VP35_EBORR VP35_EBORR] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Eborr]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amarasinghe~~, G K~~]]	+	[[Category: Amarasinghe GK]]
-	[[Category: Basler~~, C F~~]]	+	[[Category: Basler CF]]
-	[[Category: Borek~~, D M~~]]	+	[[Category: Borek DM]]
-	[[Category: Farahbakhsh, M]]	+	[[Category: Farahbakhsh M]]
-	[[Category: Leung~~, D W~~]]	+	[[Category: Leung DW]]
-	[[Category: Prins~~, K C]]~~	+	[[Category: Prins KC]]
-	~~[[Category: Host cytoplasm]]~~	+
-	~~[[Category: Interferon antiviral evasion]]~~	+
-	~~[[Category: Interferon antiviral system evasion]]~~	+
-	~~[[Category: Rna binding domain]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rna replication]]~~	+
-	~~[[Category: Rna-binding]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Virion~~]]	+

OCA at 13:37, 4 May 2022

2022-05-04T13:37:14Z

←Older revision		Revision as of 13:37, 4 May 2022
Line 1:		Line 1:

	==Crystal structure of Reston Ebola VP35 interferon inhibitory domain==		==Crystal structure of Reston Ebola VP35 interferon inhibitory domain==
-	<StructureSection load='3l2a' size='340' side='right' caption='[[3l2a]], [[Resolution\|resolution]] 1.71Å' scene=''>	+	<StructureSection load='3l2a' size='340' side='right'caption='[[3l2a]], [[Resolution\|resolution]] 1.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3l2a]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Eborr Eborr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3L2A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3l2a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Eborr Eborr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L2A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l27\|3l27]], [[3l28\|3l28]], [[3l29\|3l29]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l27\|3l27]], [[3l28\|3l28]], [[3l29\|3l29]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">REBOVgp2, VP35 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=386032 EBORR])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">REBOVgp2, VP35 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=386032 EBORR])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3l2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2a OCA], [~~http~~://pdbe.org/3l2a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3l2a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3l2a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3l2a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2a OCA], [https://pdbe.org/3l2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l2a RCSB], [https://www.ebi.ac.uk/pdbsum/3l2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l2a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/VP35_EBORR VP35_EBORR]] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).	+	[[https://www.uniprot.org/uniprot/VP35_EBORR VP35_EBORR]] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Eborr]]		[[Category: Eborr]]
		+	[[Category: Large Structures]]
	[[Category: Amarasinghe, G K]]		[[Category: Amarasinghe, G K]]
	[[Category: Basler, C F]]		[[Category: Basler, C F]]

OCA at 07:18, 4 August 2016

2016-08-04T07:18:05Z

←Older revision		Revision as of 07:18, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Reston Ebola VP35 interferon inhibitory domain==		==Crystal structure of Reston Ebola VP35 interferon inhibitory domain==
	<StructureSection load='3l2a' size='340' side='right' caption='[[3l2a]], [[Resolution\|resolution]] 1.71Å' scene=''>		<StructureSection load='3l2a' size='340' side='right' caption='[[3l2a]], [[Resolution\|resolution]] 1.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3l2a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Reston_ebolavirus Reston ebolavirus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L2A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3l2a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eborr Eborr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L2A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l27\|3l27]], [[3l28\|3l28]], [[3l29\|3l29]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l27\|3l27]], [[3l28\|3l28]], [[3l29\|3l29]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">REBOVgp2, VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~186539 Reston ebolavirus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">REBOVgp2, VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=386032 EBORR])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l2a RCSB], [http://www.ebi.ac.uk/pdbsum/3l2a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2a OCA], [http://pdbe.org/3l2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l2a RCSB], [http://www.ebi.ac.uk/pdbsum/3l2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l2a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3l2a" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Reston ebolavirus~~]]	+	[[Category: Eborr]]
	[[Category: Amarasinghe, G K]]		[[Category: Amarasinghe, G K]]
	[[Category: Basler, C F]]		[[Category: Basler, C F]]

OCA at 06:42, 24 December 2014

2014-12-24T06:42:56Z

←Older revision		Revision as of 06:42, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l2a RCSB], [http://www.ebi.ac.uk/pdbsum/3l2a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l2a RCSB], [http://www.ebi.ac.uk/pdbsum/3l2a PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VP35_EBORR VP35_EBORR]] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 14:46, 18 December 2014

2014-12-18T14:46:52Z

←Older revision		Revision as of 14:46, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3l2a\| PDB~~=3l2a \| ~~SCENE~~= }}	+	==Crystal structure of Reston Ebola VP35 interferon inhibitory domain==
-	===~~Crystal~~ structure of Reston Ebola VP35 interferon inhibitory domain~~===~~	+	<StructureSection load='3l2a' size='340' side='right' caption='[[3l2a]], [[Resolution\|resolution]] 1.71Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20399790}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3l2a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Reston_ebolavirus Reston ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L2A FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l27\|3l27]], [[3l28\|3l28]], [[3l29\|3l29]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">REBOVgp2, VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186539 Reston ebolavirus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l2a RCSB], [http://www.ebi.ac.uk/pdbsum/3l2a PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ebolaviruses are causative agents of lethal hemorrhagic fever in humans and nonhuman primates. Among the filoviruses characterized thus far, Reston Ebola virus (REBOV) is the only Ebola virus that is nonpathogenic to humans despite the fact that REBOV can cause lethal disease in nonhuman primates. Previous studies also suggest that REBOV is less effective at inhibiting host innate immune responses than Zaire Ebola virus (ZEBOV) or Marburg virus. Virally encoded VP35 protein is critical for immune suppression, but an understanding of the relative contributions of VP35 proteins from REBOV and other filoviruses is currently lacking. In order to address this question, we characterized the REBOV VP35 interferon inhibitory domain (IID) using structural, biochemical, and virological studies. These studies reveal differences in double-stranded RNA binding and interferon inhibition between the two species. These observed differences are likely due to increased stability and loss of flexibility in REBOV VP35 IID, as demonstrated by thermal shift stability assays. Consistent with this finding, the 1.71-A crystal structure of REBOV VP35 IID reveals that it is highly similar to that of ZEBOV VP35 IID, with an overall backbone r.m.s.d. of 0.64 A, but contains an additional helical element at the linker between the two subdomains of VP35 IID. Mutations near the linker, including swapping sequences between REBOV and ZEBOV, reveal that the linker sequence has limited tolerance for variability. Together with the previously solved ligand-free and double-stranded-RNA-bound forms of ZEBOV VP35 IID structures, our current studies on REBOV VP35 IID reinforce the importance of VP35 in immune suppression. Functional differences observed between REBOV and ZEBOV VP35 proteins may contribute to observed differences in pathogenicity, but these are unlikely to be the major determinant. However, the high level of similarity in structure and the low tolerance for sequence variability, coupled with the multiple critical roles played by Ebola virus VP35 proteins, highlight the viability of VP35 as a potential target for therapeutic development.

-	~~==Function==~~	+	Structural and functional characterization of Reston Ebola virus VP35 interferon inhibitory domain.,Leung DW, Shabman RS, Farahbakhsh M, Prins KC, Borek DM, Wang T, Muhlberger E, Basler CF, Amarasinghe GK J Mol Biol. 2010 Jun 11;399(3):347-57. Epub 2010 Apr 24. PMID:20399790<ref>PMID:20399790</ref>
-	~~[[http://www.uniprot.org/uniprot/VP35_EBORR VP35_EBORR]] Acts as a polymerase cofactor in the RNA polymerase transcription~~ and ~~replication complex. Prevents establishment~~ of ~~cellular antiviral state by blocking~~ virus~~-induced phosphorylation and activation of~~ interferon ~~regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta~~. ~~The mechanism by which this blockage occurs remains incompletely defined~~, ~~a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA~~. ~~Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR~~ (~~By similarity~~).	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3l2a]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Reston_ebolavirus Reston ebolavirus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:020399790</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Reston ebolavirus]]		[[Category: Reston ebolavirus]]
-	[[Category: Amarasinghe, G K.]]	+	[[Category: Amarasinghe, G K]]
-	[[Category: Basler, C F.]]	+	[[Category: Basler, C F]]
-	[[Category: Borek, D M.]]	+	[[Category: Borek, D M]]
-	[[Category: Farahbakhsh, M.]]	+	[[Category: Farahbakhsh, M]]
-	[[Category: Leung, D W.]]	+	[[Category: Leung, D W]]
-	[[Category: Prins, K C.]]	+	[[Category: Prins, K C]]
	[[Category: Host cytoplasm]]		[[Category: Host cytoplasm]]
	[[Category: Interferon antiviral evasion]]		[[Category: Interferon antiviral evasion]]

OCA: Protected "3l2a" [edit=sysop:move=sysop]

2013-04-04T20:02:04Z

Protected "3l2a" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:02, 4 April 2013

OCA at 20:02, 4 April 2013

2013-04-04T20:02:03Z

←Older revision		Revision as of 20:02, 4 April 2013
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-	{{Seed}}
-	[[Image:3l2a.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3l2a", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3l2a\| PDB=3l2a \| SCENE= }}		{{STRUCTURE_3l2a\| PDB=3l2a \| SCENE= }}
-
	===Crystal structure of Reston Ebola VP35 interferon inhibitory domain===		===Crystal structure of Reston Ebola VP35 interferon inhibitory domain===
		+	{{ABSTRACT_PUBMED_20399790}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/VP35_EBORR VP35_EBORR]] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_20399790}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~), ~~where 20399790 is~~ the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_20399790}}~~	+

	==About this Structure==		==About this Structure==
-	~~3L2A~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Reston_ebolavirus Reston ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA].	+	[[3l2a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Reston_ebolavirus Reston ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20399790~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020399790</ref><references group="xtra"/><references/>
	[[Category: Reston ebolavirus]]		[[Category: Reston ebolavirus]]
	[[Category: Amarasinghe, G K.]]		[[Category: Amarasinghe, G K.]]
Line 40:		Line 27:
	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Virion]]		[[Category: Virion]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 18 11:03:44 2010''

OCA at 06:56, 18 August 2010

2010-08-18T06:56:11Z

←Older revision		Revision as of 06:56, 18 August 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3l2a.~~jpg~~\|left\|200px]]	+	[[Image:3l2a.png\|left\|200px]]

	<!--		<!--
Line 31:		Line 31:
	[[Category: Leung, D W.]]		[[Category: Leung, D W.]]
	[[Category: Prins, K C.]]		[[Category: Prins, K C.]]
-	[[Category: Coiled coil]]
	[[Category: Host cytoplasm]]		[[Category: Host cytoplasm]]
	[[Category: Interferon antiviral evasion]]		[[Category: Interferon antiviral evasion]]
Line 42:		Line 41:
	[[Category: Virion]]		[[Category: Virion]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~May 12~~ 11:01:44 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 18 11:03:44 2010''

OCA at 08:01, 12 May 2010

2010-05-12T08:01:45Z

←Older revision		Revision as of 08:01, 12 May 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3l2a.jpg\|left\|200px]]

-	The ~~entry 3l2a~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3l2a", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3l2a\| PDB=3l2a \| SCENE= }}

-	~~Authors: Leung, D.W., Farahbakhsh, M., Borek, D.M., Prins, K.C., Basler, C.F., Amarasinghe, G.K.~~	+	===Crystal structure of Reston Ebola VP35 interferon inhibitory domain===

-	Description:

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 13 13~~:33:32 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20399790}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20399790 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20399790}}
		+
		+	==About this Structure==
		+	3L2A is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Reston_ebolavirus Reston ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2A OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20399790</ref><references group="xtra"/>
		+	[[Category: Reston ebolavirus]]
		+	[[Category: Amarasinghe, G K.]]
		+	[[Category: Basler, C F.]]
		+	[[Category: Borek, D M.]]
		+	[[Category: Farahbakhsh, M.]]
		+	[[Category: Leung, D W.]]
		+	[[Category: Prins, K C.]]
		+	[[Category: Coiled coil]]
		+	[[Category: Host cytoplasm]]
		+	[[Category: Interferon antiviral evasion]]
		+	[[Category: Interferon antiviral system evasion]]
		+	[[Category: Rna binding domain]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Rna replication]]
		+	[[Category: Rna-binding]]
		+	[[Category: Transcription]]
		+	[[Category: Virion]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 12 11:01:44 2010''

OCA at 11:33, 13 January 2010

2010-01-13T11:33:33Z

←Older revision		Revision as of 11:33, 13 January 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3l2a is ON HOLD	+	The entry 3l2a is ON HOLD until Paper Publication

	Authors: Leung, D.W., Farahbakhsh, M., Borek, D.M., Prins, K.C., Basler, C.F., Amarasinghe, G.K.		Authors: Leung, D.W., Farahbakhsh, M., Borek, D.M., Prins, K.C., Basler, C.F., Amarasinghe, G.K.
Line 7:		Line 7:
	Description:		Description:

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Dec 23 09~~:26:~~05 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 13:33:32 2010''