3m3s - Revision history

OCA at 16:28, 1 November 2023

2023-11-01T16:28:59Z

←Older revision		Revision as of 16:28, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3m3s' size='340' side='right'caption='[[3m3s]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3m3s' size='340' side='right'caption='[[3m3s]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m3s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cvhsa Cvhsa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M3S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m3s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome-related_coronavirus Severe acute respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M3S FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2qc2\|2qc2]], [[3m3t\|3m3t]], [[3m3v\|3m3v]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">main protease ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=227859 CVHSA])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3s OCA], [https://pdbe.org/3m3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m3s RCSB], [https://www.ebi.ac.uk/pdbsum/3m3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m3s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3s OCA], [https://pdbe.org/3m3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m3s RCSB], [https://www.ebi.ac.uk/pdbsum/3m3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m3s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~R1A_CVHSA R1A_CVHSA]~~] The ~~papain~~-~~like proteinase (PL~~-~~PRO~~) ~~is responsible~~ for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host ~~IRF~~-3.<ref>PMID:~~17024178~~</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> ~~The main proteinase 3CL~~-~~PRO is responsible~~ for ~~the majority of cleavages as it cleaves~~ the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]~~. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity)~~. Also ~~contains~~ an ADP-ribose-1''-phosphate (ADRP)~~-binding function~~.<ref>PMID:~~17024178~~</ref> <ref>PMID:~~17692280~~</ref> <ref>PMID:~~19369340~~</ref> ~~Nsp7-~~nsp8 hexadecamer may ~~possibly confer processivity to the polymerase~~, ~~maybe~~ by binding ~~to dsRNA or by producing primers utilized~~ by the ~~latter~~.<ref>PMID:~~17024178~~</ref> <ref>PMID:~~17692280~~</ref> <ref>PMID:~~19369340~~</ref> ~~Nsp9 is a~~ ssRNA-~~binding protein~~.<ref>PMID:~~17024178~~</ref> <ref>PMID:~~17692280~~</ref> <ref>PMID:~~19369340~~</ref>	+	[https://www.uniprot.org/uniprot/R1AB_SARS R1AB_SARS] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein. Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (PubMed:23035226). May disrupt nuclear pore function by binding and displacing host NUP93 (PubMed:30943371).<ref>PMID:23035226</ref> <ref>PMID:30943371</ref> May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.<ref>PMID:19640993</ref> Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates (PubMed:17692280). Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Nsp3, nsp4 and nsp6 together are sufficient to form DMV (PubMed:24410069). Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3 (PubMed:19369340, PubMed:24622840). Prevents also host NF-kappa-B signaling.<ref>PMID:16271890</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> <ref>PMID:24622840</ref> <ref>PMID:24410069</ref> Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Alone appears incapable to induce membrane curvature, but together with nsp3 is able to induce paired membranes. Nsp3, nsp4 and nsp6 together are sufficient to form DMV.<ref>PMID:23943763</ref> <ref>PMID:24410069</ref> Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP). May cleave host ATP6V1G1 thereby modifying host vacuoles intracellular pH.[PROSITE-ProRule:PRU00772]<ref>PMID:16226257</ref> Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Nsp3, nsp4 and nsp6 together are sufficient to form DMV (PubMed:24410069). Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes (PubMed:24991833).<ref>PMID:24991833</ref> <ref>PMID:24410069</ref> Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.<ref>PMID:22039154</ref> Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.<ref>PMID:22039154</ref> May participate in viral replication by acting as a ssRNA-binding protein.<ref>PMID:19153232</ref> Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.<ref>PMID:22635272</ref> Responsible for replication and transcription of the viral RNA genome.<ref>PMID:22791111</ref> Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.<ref>PMID:12917423</ref> <ref>PMID:22615777</ref> Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity (PubMed:16549795, PubMed:20421945, PubMed:22635272). Acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens (PubMed:23966862, PubMed:29511076, PubMed:21593585).<ref>PMID:16549795</ref> <ref>PMID:20421945</ref> <ref>PMID:21593585</ref> <ref>PMID:22635272</ref> <ref>PMID:23966862</ref> <ref>PMID:29511076</ref> Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond. Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.<ref>PMID:18417574</ref> <ref>PMID:20421945</ref>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cvhsa]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Shi, J H~~]]	+	[[Category: Severe acute respiratory syndrome-related coronavirus]]
-	[[Category: ~~Song, J X~~]]	+	[[Category: Shi JH]]
-	[[Category: ~~Hydrolase]]~~	+	[[Category: Song JX]]
-	~~[[Category: Sars coronavirus protease n214a mutation~~]]	+

OCA at 13:43, 4 May 2022

2022-05-04T13:43:47Z

←Older revision		Revision as of 13:43, 4 May 2022
Line 1:		Line 1:

	==Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus==		==Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus==
-	<StructureSection load='3m3s' size='340' side='right' caption='[[3m3s]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3m3s' size='340' side='right'caption='[[3m3s]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m3s]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3M3S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m3s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M3S FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qc2\|2qc2]], [[3m3t\|3m3t]], [[3m3v\|3m3v]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2qc2\|2qc2]], [[3m3t\|3m3t]], [[3m3v\|3m3v]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">main protease ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 CVHSA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">main protease ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 CVHSA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3s OCA], [~~http~~://pdbe.org/3m3s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3m3s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3m3s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3m3s ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3s OCA], [https://pdbe.org/3m3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m3s RCSB], [https://www.ebi.ac.uk/pdbsum/3m3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m3s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/R1A_CVHSA R1A_CVHSA]] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp9 is a ssRNA-binding protein.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>	+	[[https://www.uniprot.org/uniprot/R1A_CVHSA R1A_CVHSA]] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp9 is a ssRNA-binding protein.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>

	==See Also==		==See Also==
-	*[[~~SARS Coronavirus Main Proteinase~~\|~~SARS Coronavirus Main Proteinase~~]]	+	*[[Virus protease 3D structures\|Virus protease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Cvhsa]]		[[Category: Cvhsa]]
		+	[[Category: Large Structures]]
	[[Category: Shi, J H]]		[[Category: Shi, J H]]
	[[Category: Song, J X]]		[[Category: Song, J X]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Sars coronavirus protease n214a mutation]]		[[Category: Sars coronavirus protease n214a mutation]]

OCA at 18:33, 5 August 2016

2016-08-05T18:33:50Z

←Older revision		Revision as of 18:33, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus==		==Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus==
	<StructureSection load='3m3s' size='340' side='right' caption='[[3m3s]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3m3s' size='340' side='right' caption='[[3m3s]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m3s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sars_coronavirus Sars coronavirus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M3S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m3s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M3S FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qc2\|2qc2]], [[3m3t\|3m3t]], [[3m3v\|3m3v]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qc2\|2qc2]], [[3m3t\|3m3t]], [[3m3v\|3m3v]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">main protease ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 ~~SARS coronavirus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">main protease ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 CVHSA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m3s RCSB], [http://www.ebi.ac.uk/pdbsum/3m3s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3s OCA], [http://pdbe.org/3m3s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m3s RCSB], [http://www.ebi.ac.uk/pdbsum/3m3s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m3s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sars coronavirus~~]]	+	[[Category: Cvhsa]]
	[[Category: Shi, J H]]		[[Category: Shi, J H]]
	[[Category: Song, J X]]		[[Category: Song, J X]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Sars coronavirus protease n214a mutation]]		[[Category: Sars coronavirus protease n214a mutation]]

OCA at 20:59, 25 December 2014

2014-12-25T20:59:54Z

←Older revision		Revision as of 20:59, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m3s RCSB], [http://www.ebi.ac.uk/pdbsum/3m3s PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m3s RCSB], [http://www.ebi.ac.uk/pdbsum/3m3s PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/R1A_CVHSA R1A_CVHSA]] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp9 is a ssRNA-binding protein.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>

	==See Also==		==See Also==
	*[[SARS Coronavirus Main Proteinase\|SARS Coronavirus Main Proteinase]]		*[[SARS Coronavirus Main Proteinase\|SARS Coronavirus Main Proteinase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:59, 9 December 2014

2014-12-09T08:59:08Z

←Older revision		Revision as of 08:59, 9 December 2014
Line 1:		Line 1:
-	~~[[Image:3m3s.png\|left\|200px]]~~	+	==Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus==
-		+	<StructureSection load='3m3s' size='340' side='right' caption='[[3m3s]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{STRUCTURE_3m3s\| PDB=3m3s \| SCENE= }}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3m3s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M3S FirstGlance]. <br>
-	===Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus===	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qc2\|2qc2]], [[3m3t\|3m3t]], [[3m3v\|3m3v]]</td></tr>
-		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">main protease ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 SARS coronavirus])</td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m3s RCSB], [http://www.ebi.ac.uk/pdbsum/3m3s PDBsum]</span></td></tr>
-	==~~About this Structure~~==	+	</table>
-	[[3m3s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA].	+

	==See Also==		==See Also==
	*[[SARS Coronavirus Main Proteinase\|SARS Coronavirus Main Proteinase]]		*[[SARS Coronavirus Main Proteinase\|SARS Coronavirus Main Proteinase]]
		+	__TOC__
		+	</StructureSection>
	[[Category: Sars coronavirus]]		[[Category: Sars coronavirus]]
-	[[Category: Shi, J H.]]	+	[[Category: Shi, J H]]
-	[[Category: Song, J X.]]	+	[[Category: Song, J X]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Sars coronavirus protease n214a mutation]]		[[Category: Sars coronavirus protease n214a mutation]]

OCA at 21:34, 20 October 2012

2012-10-20T21:34:55Z

←Older revision		Revision as of 21:34, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3m3s]] is a 2 chain structure ~~of [[SARS Coronavirus Main Proteinase]]~~ with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA].	+	[[3m3s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA].

	==See Also==		==See Also==

OCA at 13:40, 27 July 2012

2012-07-27T13:40:20Z

←Older revision		Revision as of 13:40, 27 July 2012
Line 1:		Line 1:
-	[[Image:3m3s.~~jpg~~\|left\|200px]]	+	[[Image:3m3s.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3m3s", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3m3s\| PDB=3m3s \| SCENE= }}		{{STRUCTURE_3m3s\| PDB=3m3s \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3m3s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA].	+	[[3m3s]] is a 2 chain structure of [[SARS Coronavirus Main Proteinase]] with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA].
		+
		+	==See Also==
		+	*[[SARS Coronavirus Main Proteinase\|SARS Coronavirus Main Proteinase]]
	[[Category: Sars coronavirus]]		[[Category: Sars coronavirus]]
	[[Category: Shi, J H.]]		[[Category: Shi, J H.]]
	[[Category: Song, J X.]]		[[Category: Song, J X.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Sars coronavirus protease n214a mutation]]

OCA: Protected "3m3s" [edit=sysop:move=sysop]

2011-03-23T07:15:16Z

Protected "3m3s" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:15, 23 March 2011

OCA at 07:15, 23 March 2011

2011-03-23T07:15:13Z

←Older revision		Revision as of 07:15, 23 March 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3m3s.jpg\|left\|200px]]

-	The ~~entry 3m3s~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3m3s", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3m3s\| PDB=3m3s \| SCENE= }}

-	~~Authors: Shi, J.H., Song, J.X.~~	+	===Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus===

-	Description: Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Mar 31 13~~:22:~~36 2010''~~	+	==About this Structure==
		+	[[3m3s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3S OCA].
		+	[[Category: Sars coronavirus]]
		+	[[Category: Shi, J H.]]
		+	[[Category: Song, J X.]]

OCA at 10:22, 31 March 2010

2010-03-31T10:22:36Z

←Older revision		Revision as of 10:22, 31 March 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3m3s is ON HOLD	+	The entry 3m3s is ON HOLD until Paper Publication

	Authors: Shi, J.H., Song, J.X.		Authors: Shi, J.H., Song, J.X.
Line 7:		Line 7:
	Description: Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus		Description: Crystal structure of SARS-COV main protease Asn214Ala mutant with authorize N-terminus

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar ~~17 09:~~13:27 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 31 13:22:36 2010''