3m7q - Revision history

OCA at 08:49, 6 September 2023

2023-09-06T08:49:26Z

←Older revision		Revision as of 08:49, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3m7q' size='340' side='right'caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3m7q' size='340' side='right'caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/~~Stihl Stihl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [https://pdbe.org/3m7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [https://www.ebi.ac.uk/pdbsum/3m7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [https://pdbe.org/3m7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [https://www.ebi.ac.uk/pdbsum/3m7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~ISH1_STOHE ISH1_STOHE~~]~~] Active against serine, cysteine, and aspartic proteinases. Can bind vertebrate trypsin and chymotrypsin.<ref>PMID:9027993</ref> <ref>PMID:22975140</ref>~~	+	[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 28:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Stihl]]~~	+	[[Category: Stichodactyla helianthus]]
-	~~[[Category: Trypsin~~]]	+	[[Category: Betzel C]]
-	[[Category: Betzel, C]]	+	[[Category: Garcia-Fernandez R]]
-	~~[[Category: Chavez, M de los angeles~~]]	+	[[Category: Gil D]]
-	[[Category: Garcia-Fernandez, R]]	+	[[Category: Gonzalez Y]]
-	[[Category: Gil, D]]	+	[[Category: Perbandt M]]
-	[[Category: Gonzalez, Y]]	+	[[Category: Pons T]]
-	[[Category: Perbandt, M]]	+	[[Category: Redecke L]]
-	[[Category: Pons, T]]	+	[[Category: Talavera A]]
-	[[Category: Redecke, L]]	+	[[Category: De los angeles Chavez M]]
-	[[Category: Talavera, A]]	+
-	[[Category: ~~Digestion]]~~	+
-	~~[[Category: Disulfide bond]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Kunitz-type serine-protease inhibitor]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Nematocyst]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Protease inhibitor]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Serine protease]]~~	+
-	~~[[Category: Serine protease inhibitor]]~~	+
-	~~[[Category: Trypsin-inhibitor complex]]~~	+
-	~~[[Category: Zymogen~~]]	+

OCA at 07:33, 20 July 2022

2022-07-20T07:33:11Z

←Older revision		Revision as of 07:33, 20 July 2022
Line 3:		Line 3:
	<StructureSection load='3m7q' size='340' side='right'caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3m7q' size='340' side='right'caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [~~http~~://en.wikipedia.org/wiki/Stihl Stihl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Stihl Stihl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [~~http~~://pdbe.org/3m7q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3m7q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [https://pdbe.org/3m7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [https://www.ebi.ac.uk/pdbsum/3m7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ISH1_STOHE ISH1_STOHE]] Active against serine, cysteine, and aspartic proteinases. Can bind vertebrate trypsin and chymotrypsin.<ref>PMID:9027993</ref> <ref>PMID:22975140</ref>	+	[[https://www.uniprot.org/uniprot/ISH1_STOHE ISH1_STOHE]] Active against serine, cysteine, and aspartic proteinases. Can bind vertebrate trypsin and chymotrypsin.<ref>PMID:9027993</ref> <ref>PMID:22975140</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:40, 14 October 2020

2020-10-14T06:40:01Z

←Older revision		Revision as of 06:40, 14 October 2020
Line 1:		Line 1:

	==Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin==		==Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin==
-	<StructureSection load='3m7q' size='340' side='right' caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3m7q' size='340' side='right'caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Stihl Stihl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Stihl Stihl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [http://pdbe.org/3m7q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [http://www.ebi.ac.uk/pdbsum/3m7q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [http://pdbe.org/3m7q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [http://www.ebi.ac.uk/pdbsum/3m7q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Trypsin\|Trypsin]]	+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
	[[Category: Stihl]]		[[Category: Stihl]]
	[[Category: Trypsin]]		[[Category: Trypsin]]

OCA at 07:33, 9 January 2019

2019-01-09T07:33:12Z

←Older revision		Revision as of 07:33, 9 January 2019
Line 3:		Line 3:
	<StructureSection load='3m7q' size='340' side='right' caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3m7q' size='340' side='right' caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/~~Carribean_sea_anemone Carribean sea anemone~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Stihl Stihl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
-	[[Category: ~~Carribean sea anemone~~]]	+	[[Category: Stihl]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Betzel, C]]		[[Category: Betzel, C]]

OCA at 18:43, 4 August 2016

2016-08-04T18:43:05Z

←Older revision		Revision as of 18:43, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin==		==Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin==
	<StructureSection load='3m7q' size='340' side='right' caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3m7q' size='340' side='right' caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/~~Stichodactyla_helianthus Stichodactyla helianthus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Carribean_sea_anemone Carribean sea anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [http://www.ebi.ac.uk/pdbsum/3m7q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [http://pdbe.org/3m7q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [http://www.ebi.ac.uk/pdbsum/3m7q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3m7q" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
-	[[Category: ~~Stichodactyla helianthus~~]]	+	[[Category: Carribean sea anemone]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Betzel, C]]		[[Category: Betzel, C]]

OCA at 12:03, 25 December 2014

2014-12-25T12:03:27Z

←Older revision		Revision as of 12:03, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [http://www.ebi.ac.uk/pdbsum/3m7q PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [http://www.ebi.ac.uk/pdbsum/3m7q PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ISH1_STOHE ISH1_STOHE]] Active against serine, cysteine, and aspartic proteinases. Can bind vertebrate trypsin and chymotrypsin.<ref>PMID:9027993</ref> <ref>PMID:22975140</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:41, 9 December 2014

2014-12-09T09:41:57Z

←Older revision		Revision as of 09:41, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3m7q.~~png~~\|~~left\|200px~~]]	+	==Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin==
		+	<StructureSection load='3m7q' size='340' side='right' caption='[[3m7q]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3m7q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M7Q FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m7q RCSB], [http://www.ebi.ac.uk/pdbsum/3m7q PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins isolated from marine invertebrates are frequently characterized by exceptional structural and functional properties. ShPI-1, a BPTI Kunitz-type inhibitor from the Caribbean Sea anemone Stichodactyla helianthus, displays activity not only against serine-, but also against cysteine-, and aspartate proteases. As an initial step to evaluate the molecular basis of its activities, we describe the crystallographic structure of ShPI-1 in complex with the serine protease bovine pancreatic trypsin at 1.7A resolution. The overall structure and the important enzyme-inhibitor interactions of this first invertebrate BPTI-like Kunitz-type inhibitor:trypsin complex remained largely conserved compared to mammalian BPTI-Kunitz inhibitor complexes. However, a prominent stabilizing role within the interface was attributed to arginine at position P3. Binding free-energy calculations indicated a 10-fold decrease for the inhibitor affinity against trypsin, if the P3 residue of ShPI-1 is mutated to alanine. Together with the increased role of Arg(11) at P3 position, slightly reduced interactions at the prime side (Pn') of the primary binding loop and at the secondary binding loop of ShPI-1 were detected. In addition, the structure provides important information for site directed mutagenesis to further optimize the activity of rShPI-1A for biotechnological applications.

-	~~{{STRUCTURE_3m7q\| PDB=3m7q \| SCENE= }}~~	+	Structural insights into serine protease inhibition by a marine invertebrate BPTI Kunitz-type inhibitor.,Garcia-Fernandez R, Pons T, Perbandt M, Valiente PA, Talavera A, Gonzalez-Gonzalez Y, Rehders D, Chavez MA, Betzel C, Redecke L J Struct Biol. 2012 Sep 5. pii: S1047-8477(12)00240-7. doi:, 10.1016/j.jsb.2012.08.009. PMID:22975140<ref>PMID:22975140</ref>

-	~~===Crystal structure~~ of ~~recombinant Kunitz Type serine protease Inhibitor-1 from~~ the ~~Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22975140}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3m7q]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus] and [http://en~~.~~wikipedia.org~~/~~wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA].~~	+

	==See Also==		==See Also==
	*[[Trypsin\|Trypsin]]		*[[Trypsin\|Trypsin]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Stichodactyla helianthus]]		[[Category: Stichodactyla helianthus]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
-	[[Category: Betzel, C.]]	+	[[Category: Betzel, C]]
-	[[Category: Chavez, M de los angeles.]]	+	[[Category: Chavez, M de los angeles]]
-	[[Category: Garcia-Fernandez, R.]]	+	[[Category: Garcia-Fernandez, R]]
-	[[Category: Gil, D.]]	+	[[Category: Gil, D]]
-	[[Category: Gonzalez, Y.]]	+	[[Category: Gonzalez, Y]]
-	[[Category: Perbandt, M.]]	+	[[Category: Perbandt, M]]
-	[[Category: Pons, T.]]	+	[[Category: Pons, T]]
-	[[Category: Redecke, L.]]	+	[[Category: Redecke, L]]
-	[[Category: Talavera, A.]]	+	[[Category: Talavera, A]]
	[[Category: Digestion]]		[[Category: Digestion]]
	[[Category: Disulfide bond]]		[[Category: Disulfide bond]]

OCA at 10:43, 14 November 2012

2012-11-14T10:43:07Z

←Older revision		Revision as of 10:43, 14 November 2012
Line 5:		Line 5:
	===Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin===		===Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin===

		+	{{ABSTRACT_PUBMED_22975140}}

	==About this Structure==		==About this Structure==

OCA at 07:05, 14 November 2012

2012-11-14T07:05:41Z

←Older revision		Revision as of 07:05, 14 November 2012
Line 5:		Line 5:
	===Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin===		===Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Caribbean sea anemone stichodactyla helianthus in complex with bovine pancreatic trypsin===

-	{{ABSTRACT_PUBMED_22975140}}

	==About this Structure==		==About this Structure==

OCA at 16:27, 20 October 2012

2012-10-20T16:27:06Z

←Older revision		Revision as of 16:27, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3m7q]] is a 2 chain structure ~~of [[Trypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA].	+	[[3m7q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7Q OCA].

	==See Also==		==See Also==