3m7t - Revision history

OCA at 07:55, 12 July 2023

2023-07-12T07:55:32Z

←Older revision		Revision as of 07:55, 12 July 2023
Line 3:		Line 3:
	<StructureSection load='3m7t' size='340' side='right'caption='[[3m7t]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3m7t' size='340' side='right'caption='[[3m7t]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m7t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_29487 Atcc 29487~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m7t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M7T FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3m7u\|3m7u]], [[1ssx\|1ssx]], [[1tal\|1tal]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>alpha-LP, Lysobacter ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~69 ATCC 29487])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Alpha-lytic_endopeptidase Alpha-lytic endopeptidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.12 3.4.21.12]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7t OCA], [https://pdbe.org/3m7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m7t RCSB], [https://www.ebi.ac.uk/pdbsum/3m7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7t OCA], [https://pdbe.org/3m7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m7t RCSB], [https://www.ebi.ac.uk/pdbsum/3m7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7t ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/PRLA_LYSEN PRLA_LYSEN]
-	The crystal structure of the extracellular bacterial serine protease alpha-lytic protease (alphaLP) has been solved at 0.83 A resolution at pH 8. This ultra-high resolution structure allows accurate analysis of structural elements not possible with previous structures. Hydrogen atoms are visible, and confirm active-site hydrogen-bonding interactions expected for the apo enzyme. In particular, His57 N(delta1) participates in a normal hydrogen bond with Asp102 in the catalytic triad, with a hydrogen atom visible 0.83(+/-0.06)A from the His N(delta1). The catalytic Ser195 occupies two conformations, one corresponding to a population of His57 that is doubly protonated, the other to the singly protonated His57. Based on the occupancy of these conformations, the pKa of His57 is calculated to be approximately 8.8 when a sulfate ion occupies the active site. This 0.83 A structure has allowed critical analysis of geometric distortions within the structure. Interestingly, Phe228 is significantly distorted from planarity. The distortion of Phe228, buried in the core of the C-terminal domain, occurs at an estimated energetic cost of 4.1 kcal/mol. The conformational space for Phe228 is severely limited by the presence of Trp199, which prevents Phe228 from adopting the rotamer observed in many other chymotrypsin family members. In alphaLP, the only allowed rotamer leads to the deformation of Phe228 due to steric interactions with Thr181. We hypothesize that tight packing of co-evolved residues in this region, and the subsequent deformation of Phe228, contributes to the high cooperativity and large energetic barriers for folding and unfolding of alphaLP. The kinetic stability imparted by the large, cooperative unfolding barrier plays a critical role in extending the lifetime of the protease in its harsh environment.	+
-		+
-	The 0.83 A resolution crystal structure of alpha-lytic protease reveals the detailed structure of the active site and identifies a source of conformational strain.,Fuhrmann CN, Kelch BA, Ota N, Agard DA J Mol Biol. 2004 May 14;338(5):~~999-1013. PMID:15111063<ref>PMID:15111063<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3m7t" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Alpha-lytic protease 3D structures\|Alpha-lytic protease 3D structures]]		*[[Alpha-lytic protease 3D structures\|Alpha-lytic protease 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Alpha-lytic endopeptidase]]
-	[[Category: Atcc 29487]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Agard, D A~~]]	+	[[Category: Lysobacter enzymogenes]]
-	[[Category: ~~Bailey, F P.Erciyas~~]]	+	[[Category: Agard DA]]
-	[[Category: ~~Waddling, C A~~]]	+	[[Category: Erciyas Bailey FP]]
-	[[Category: ~~Disulfide bond]]~~	+	[[Category: Waddling CA]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Serine protease]]~~	+
-	~~[[Category: Zymogen~~]]	+

OCA at 05:38, 6 October 2021

2021-10-06T05:38:18Z

←Older revision		Revision as of 05:38, 6 October 2021
Line 1:		Line 1:

	==Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant==		==Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant==
-	<StructureSection load='3m7t' size='340' side='right' caption='[[3m7t]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='3m7t' size='340' side='right'caption='[[3m7t]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m7t]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3M7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m7t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M7T FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m7u\|3m7u]], [[1ssx\|1ssx]], [[1tal\|1tal]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3m7u\|3m7u]], [[1ssx\|1ssx]], [[1tal\|1tal]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">alpha-LP, Lysobacter ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 ATCC 29487])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">alpha-LP, Lysobacter ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 ATCC 29487])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Alpha-lytic_endopeptidase Alpha-lytic endopeptidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.12 3.4.21.12] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha-lytic_endopeptidase Alpha-lytic endopeptidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.12 3.4.21.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3m7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7t OCA], [~~http~~://pdbe.org/3m7t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3m7t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3m7t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7t ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7t OCA], [https://pdbe.org/3m7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m7t RCSB], [https://www.ebi.ac.uk/pdbsum/3m7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Alpha-lytic protease\|Alpha-lytic protease]]	+	*[[Alpha-lytic protease 3D structures\|Alpha-lytic protease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	[[Category: Alpha-lytic endopeptidase]]		[[Category: Alpha-lytic endopeptidase]]
	[[Category: Atcc 29487]]		[[Category: Atcc 29487]]
		+	[[Category: Large Structures]]
	[[Category: Agard, D A]]		[[Category: Agard, D A]]
	[[Category: Bailey, F P.Erciyas]]		[[Category: Bailey, F P.Erciyas]]

OCA at 12:14, 4 August 2016

2016-08-04T12:14:56Z

←Older revision		Revision as of 12:14, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant==		==Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant==
	<StructureSection load='3m7t' size='340' side='right' caption='[[3m7t]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3m7t' size='340' side='right' caption='[[3m7t]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lysobacter_enzymogenes Lysobacter enzymogenes~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M7T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m7u\|3m7u]], [[1ssx\|1ssx]], [[1tal\|1tal]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m7u\|3m7u]], [[1ssx\|1ssx]], [[1tal\|1tal]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">alpha-LP, Lysobacter ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 ~~Lysobacter enzymogenes~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">alpha-LP, Lysobacter ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 ATCC 29487])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-lytic_endopeptidase Alpha-lytic endopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.12 3.4.21.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-lytic_endopeptidase Alpha-lytic endopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.12 3.4.21.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m7t RCSB], [http://www.ebi.ac.uk/pdbsum/3m7t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7t OCA], [http://pdbe.org/3m7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m7t RCSB], [http://www.ebi.ac.uk/pdbsum/3m7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m7t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3m7t" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Alpha-lytic endopeptidase]]		[[Category: Alpha-lytic endopeptidase]]
-	[[Category: ~~Lysobacter enzymogenes~~]]	+	[[Category: Atcc 29487]]
	[[Category: Agard, D A]]		[[Category: Agard, D A]]
	[[Category: Bailey, F P.Erciyas]]		[[Category: Bailey, F P.Erciyas]]

OCA at 14:53, 18 December 2014

2014-12-18T14:53:02Z

←Older revision		Revision as of 14:53, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3m7t\| PDB=3m7t \| SCENE= }}~~	+	==Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant==
-	===Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant===	+	<StructureSection load='3m7t' size='340' side='right' caption='[[3m7t]], [[Resolution\|resolution]] 1.55Å' scene=''>
-	~~{{ABSTRACT_PUBMED_15111063}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3m7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M7T FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3m7u\|3m7u]], [[1ssx\|1ssx]], [[1tal\|1tal]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">alpha-LP, Lysobacter ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 Lysobacter enzymogenes])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-lytic_endopeptidase Alpha-lytic endopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.12 3.4.21.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m7t RCSB], [http://www.ebi.ac.uk/pdbsum/3m7t PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the extracellular bacterial serine protease alpha-lytic protease (alphaLP) has been solved at 0.83 A resolution at pH 8. This ultra-high resolution structure allows accurate analysis of structural elements not possible with previous structures. Hydrogen atoms are visible, and confirm active-site hydrogen-bonding interactions expected for the apo enzyme. In particular, His57 N(delta1) participates in a normal hydrogen bond with Asp102 in the catalytic triad, with a hydrogen atom visible 0.83(+/-0.06)A from the His N(delta1). The catalytic Ser195 occupies two conformations, one corresponding to a population of His57 that is doubly protonated, the other to the singly protonated His57. Based on the occupancy of these conformations, the pKa of His57 is calculated to be approximately 8.8 when a sulfate ion occupies the active site. This 0.83 A structure has allowed critical analysis of geometric distortions within the structure. Interestingly, Phe228 is significantly distorted from planarity. The distortion of Phe228, buried in the core of the C-terminal domain, occurs at an estimated energetic cost of 4.1 kcal/mol. The conformational space for Phe228 is severely limited by the presence of Trp199, which prevents Phe228 from adopting the rotamer observed in many other chymotrypsin family members. In alphaLP, the only allowed rotamer leads to the deformation of Phe228 due to steric interactions with Thr181. We hypothesize that tight packing of co-evolved residues in this region, and the subsequent deformation of Phe228, contributes to the high cooperativity and large energetic barriers for folding and unfolding of alphaLP. The kinetic stability imparted by the large, cooperative unfolding barrier plays a critical role in extending the lifetime of the protease in its harsh environment.

-	~~==About this Structure==~~	+	The 0.83 A resolution crystal structure of alpha-lytic protease reveals the detailed structure of the active site and identifies a source of conformational strain.,Fuhrmann CN, Kelch BA, Ota N, Agard DA J Mol Biol. 2004 May 14;338(5):999-1013. PMID:15111063<ref>PMID:15111063</ref>
-	~~[[3m7t]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3M7T OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:015111063</ref><ref group~~=~~"xtra">PMID:009232638</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[Alpha-lytic protease\|Alpha-lytic protease]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Alpha-lytic endopeptidase]]		[[Category: Alpha-lytic endopeptidase]]
	[[Category: Lysobacter enzymogenes]]		[[Category: Lysobacter enzymogenes]]
-	[[Category: Agard, D A.]]	+	[[Category: Agard, D A]]
-	[[Category: Bailey, F P.Erciyas.]]	+	[[Category: Bailey, F P.Erciyas]]
-	[[Category: Waddling, C A.]]	+	[[Category: Waddling, C A]]
	[[Category: Disulfide bond]]		[[Category: Disulfide bond]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 23:13, 10 April 2013

2013-04-10T23:13:14Z

←Older revision		Revision as of 23:13, 10 April 2013
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-	[[Image:3m7t.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3m7t", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3m7t\| PDB=3m7t \| SCENE= }}		{{STRUCTURE_3m7t\| PDB=3m7t \| SCENE= }}
-
	===Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant===		===Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15111063}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15111063 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15111063}}		{{ABSTRACT_PUBMED_15111063}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15111063~~</ref><ref group="xtra">PMID:~~9232638~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015111063</ref><ref group="xtra">PMID:009232638</ref><references group="xtra"/><references/>
	[[Category: Alpha-lytic endopeptidase]]		[[Category: Alpha-lytic endopeptidase]]
	[[Category: Lysobacter enzymogenes]]		[[Category: Lysobacter enzymogenes]]
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	[[Category: Bailey, F P.Erciyas.]]		[[Category: Bailey, F P.Erciyas.]]
	[[Category: Waddling, C A.]]		[[Category: Waddling, C A.]]
		+	[[Category: Disulfide bond]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protease]]
		+	[[Category: Serine protease]]
		+	[[Category: Zymogen]]

OCA at 17:58, 14 March 2011

2011-03-14T17:58:59Z

←Older revision		Revision as of 17:58, 14 March 2011
Line 1:		Line 1:
-	[[Image:3m7t.~~jpg~~\|left\|200px]]	+	[[Image:3m7t.png\|left\|200px]]

	<!--		<!--
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	===Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant===		===Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_15111063}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15111063 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15111063}}

	==About this Structure==		==About this Structure==

OCA: Protected "3m7t" [edit=sysop:move=sysop]

2011-02-09T06:07:24Z

Protected "3m7t" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:07, 9 February 2011

OCA at 06:07, 9 February 2011

2011-02-09T06:07:21Z

←Older revision		Revision as of 06:07, 9 February 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3m7t.jpg\|left\|200px]]

-	The ~~entry 3m7t~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3m7t", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3m7t\| PDB=3m7t \| SCENE= }}

-	~~Authors: Agard, D.A., Erciyas Bailey, F.P., Waddling, C.A.~~	+	===Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant===

-	Description: Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Apr 21 09~~:36:~~06 2010''~~	+	==About this Structure==
		+	[[3m7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M7T OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:15111063</ref><ref group="xtra">PMID:9232638</ref><references group="xtra"/>
		+	[[Category: Alpha-lytic endopeptidase]]
		+	[[Category: Lysobacter enzymogenes]]
		+	[[Category: Agard, D A.]]
		+	[[Category: Bailey, F P.Erciyas.]]
		+	[[Category: Waddling, C A.]]

OCA at 06:36, 21 April 2010

2010-04-21T06:36:07Z

←Older revision		Revision as of 06:36, 21 April 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3m7t is ON HOLD	+	The entry 3m7t is ON HOLD until Paper Publication

	Authors: Agard, D.A., Erciyas Bailey, F.P., Waddling, C.A.		Authors: Agard, D.A., Erciyas Bailey, F.P., Waddling, C.A.
Line 7:		Line 7:
	Description: Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant		Description: Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 24 08~~:39:27 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 21 09:36:06 2010''

OCA: New page: '''Unreleased structure''' The entry 3m7t is ON HOLD Authors: Agard, D.A., Erciyas Bailey, F.P., Waddling, C.A. Description: Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S M...

2010-03-24T06:39:28Z

New page: '''Unreleased structure''' The entry 3m7t is ON HOLD Authors: Agard, D.A., Erciyas Bailey, F.P., Waddling, C.A. Description: Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S M...

New page

'''Unreleased structure'''

The entry 3m7t is ON HOLD

Authors: Agard, D.A., Erciyas Bailey, F.P., Waddling, C.A.

Description: Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 24 08:39:27 2010''