3msi - Revision history

OCA at 06:48, 9 August 2023

2023-08-09T06:48:43Z

←Older revision		Revision as of 06:48, 9 August 2023
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	<StructureSection load='3msi' size='340' side='right'caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='3msi' size='340' side='right'caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Blennius_americanus Blennius~~ americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MSI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MSI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [https://pdbe.org/3msi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [https://www.ebi.ac.uk/pdbsum/3msi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3msi ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.43Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [https://pdbe.org/3msi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [https://www.ebi.ac.uk/pdbsum/3msi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3msi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ANP12_ZOAAM ANP12_ZOAAM] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Blennius americanus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Davies, P L~~]]	+	[[Category: Zoarces americanus]]
-	[[Category: ~~Deluca, C I~~]]	+	[[Category: Davies PL]]
-	[[Category: ~~Jia, Z~~]]	+	[[Category: Deluca CI]]
-	[[Category: ~~Ye, Q~~]]	+	[[Category: Jia Z]]
-	[[Category: ~~Antifreeze]]~~	+	[[Category: Ye Q]]
-	~~[[Category: Antifreeze polypeptide]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Ice binding protein]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Thermal hysteresis protein~~]]	+

OCA at 15:47, 3 November 2021

2021-11-03T15:47:40Z

←Older revision		Revision as of 15:47, 3 November 2021
Line 3:		Line 3:
	<StructureSection load='3msi' size='340' side='right'caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='3msi' size='340' side='right'caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3MSI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MSI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [~~http~~://pdbe.org/3msi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3msi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3msi ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [https://pdbe.org/3msi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [https://www.ebi.ac.uk/pdbsum/3msi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3msi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:59, 21 October 2020

2020-10-21T08:59:07Z

←Older revision		Revision as of 08:59, 21 October 2020
Line 1:		Line 1:

	==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12==		==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12==
-	<StructureSection load='3msi' size='340' side='right' caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>	+	<StructureSection load='3msi' size='340' side='right'caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3MSI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3MSI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [http://pdbe.org/3msi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [http://www.ebi.ac.uk/pdbsum/3msi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3msi ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [http://pdbe.org/3msi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [http://www.ebi.ac.uk/pdbsum/3msi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3msi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:

	==See Also==		==See Also==
-	*[[Antifreeze protein\|Antifreeze protein]]	+	*[[Antifreeze protein 3D structures\|Antifreeze protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Blennius americanus]]		[[Category: Blennius americanus]]
		+	[[Category: Large Structures]]
	[[Category: Davies, P L]]		[[Category: Davies, P L]]
	[[Category: Deluca, C I]]		[[Category: Deluca, C I]]

OCA at 13:32, 16 January 2019

2019-01-16T13:32:01Z

←Older revision		Revision as of 13:32, 16 January 2019
Line 1:		Line 1:
		+
	==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12==		==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12==
	<StructureSection load='3msi' size='340' side='right' caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='3msi' size='340' side='right' caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MSI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MSI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [http://pdbe.org/3msi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [http://www.ebi.ac.uk/pdbsum/3msi PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [http://pdbe.org/3msi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [http://www.ebi.ac.uk/pdbsum/3msi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3msi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 9:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/3msi_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/3msi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:27, 7 February 2016

2016-02-07T15:27:11Z

←Older revision		Revision as of 15:27, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3msi' size='340' side='right' caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='3msi' size='340' side='right' caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Zoarces_americanus Zoarces~~ americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MSI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MSI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [http://www.ebi.ac.uk/pdbsum/3msi PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [http://pdbe.org/3msi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [http://www.ebi.ac.uk/pdbsum/3msi PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3msi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3msi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Zoarces~~ americanus]]	+	[[Category: Blennius americanus]]
	[[Category: Davies, P L]]		[[Category: Davies, P L]]
	[[Category: Deluca, C I]]		[[Category: Deluca, C I]]

OCA at 16:17, 24 December 2014

2014-12-24T16:17:41Z

←Older revision		Revision as of 16:17, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MSI FirstGlance]. <br>		<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MSI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [http://www.ebi.ac.uk/pdbsum/3msi PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [http://www.ebi.ac.uk/pdbsum/3msi PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Zoarces americanus]]		[[Category: Zoarces americanus]]
-	[[Category: Davies, P L.]]	+	[[Category: Davies, P L]]
-	[[Category: Deluca, C I.]]	+	[[Category: Deluca, C I]]
-	[[Category: Jia, Z.]]	+	[[Category: Jia, Z]]
-	[[Category: Ye, Q.]]	+	[[Category: Ye, Q]]
	[[Category: Antifreeze]]		[[Category: Antifreeze]]
	[[Category: Antifreeze polypeptide]]		[[Category: Antifreeze polypeptide]]

OCA at 12:33, 29 September 2014

2014-09-29T12:33:07Z

←Older revision		Revision as of 12:33, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:3msi.~~png\|left~~\|200px]]	+	==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12==
		+	<StructureSection load='3msi' size='340' side='right' caption='[[3msi]], [[Resolution\|resolution]] 1.43Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MSI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3msi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3msi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3msi RCSB], [http://www.ebi.ac.uk/pdbsum/3msi PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/3msi_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The interaction of proteins with ice is poorly understood and difficult to study, partly because ice is transitory and can present many binding surfaces, and partly because structures have been determined for only two ice-binding proteins. This paper focuses on one of these, a 66-residue antifreeze protein (AFP) from eel pout. The high resolution X-ray structure of this fish AFP demonstrated that the proposed ice-binding surface is remarkably flat for such a small protein. The residues on the planar surface thought to be involved in ice binding are restrained by hydrogen bonds or by tight packing of their side-chains. To probe the requirement for a flat binding surface, a conserved alanine in the center of the AFP planar surface was substituted with larger residues. Six alanine replacement mutants (Ala16 > Cys, Thr, Met, Arg, His and Tyr), designed to disrupt the planarity of the surface and sterically block binding to ice, were characterized by X-ray crystallography and compared with the wild-type AFP. In each case, the detail provided by these crystal structures has helped explain the effects of the mutation on antifreeze activity. The substitutions, Ala16 > His and Ala16 > Tyr, were large enough to shield Gln44, one of the putative ice-binding residues, contributing to their very low thermal hysteresis activity. In addition to sterically hindering the putative ice-binding site, the bulkier residues also caused shifts in the putative ice-binding residues owing to the tight packing of side-chains on the planar surface. This unexpected consequence of the mutations helps account for the severely reduced antifreeze activity. One explanation for residual antifreeze activity in some of the mutants lies in the possibility that AFPs have a role in shaping the site on the ice to which they bind. Thus, side-chain dislocations might be partially accommodated by ice that can freeze around them. It is evident that the disruption of the planarity, by introducing larger residues at the center of the proposed ice-binding site, is not the only factor responsible for the loss of antifreeze activity. There are multiple causes including positional change and steric blockage of some putative ice-binding residues.

-	~~{{STRUCTURE_3msi\| PDB=3msi \| SCENE= }}~~	+	The effects of steric mutations on the structure of type III antifreeze protein and its interaction with ice.,DeLuca CI, Davies PL, Ye Q, Jia Z J Mol Biol. 1998 Jan 23;275(3):515-25. PMID:9466928<ref>PMID:9466928</ref>

-	~~===TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9466928}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3msi]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Zoarces_americanus Zoarces americanus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA].~~	+

	==See Also==		==See Also==
	*[[Antifreeze protein\|Antifreeze protein]]		*[[Antifreeze protein\|Antifreeze protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009466928<~~/~~ref~~><~~ref group="xtra">PMID:011742118</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Zoarces americanus]]		[[Category: Zoarces americanus]]
	[[Category: Davies, P L.]]		[[Category: Davies, P L.]]

OCA at 07:03, 20 October 2012

2012-10-20T07:03:57Z

←Older revision		Revision as of 07:03, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3msi]] is a 1 chain structure ~~of [[Antifreeze protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA].	+	[[3msi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA].

	==See Also==		==See Also==

OCA: Protected "3msi" [edit=sysop:move=sysop]

2012-07-25T15:10:00Z

Protected "3msi" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:10, 25 July 2012

OCA at 15:09, 25 July 2012

2012-07-25T15:09:58Z

←Older revision		Revision as of 15:09, 25 July 2012
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-	{{Seed}}
	[[Image:3msi.png\|left\|200px]]		[[Image:3msi.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3msi", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_3msi\| PDB=3msi \| SCENE= }}		{{STRUCTURE_3msi\| PDB=3msi \| SCENE= }}

	===TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12===		===TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9466928}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9466928 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_9466928}}		{{ABSTRACT_PUBMED_9466928}}

	==About this Structure==		==About this Structure==
-	~~3MSI~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/~~Macrozoarces_americanus Macrozoarces~~ americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA].	+	[[3msi]] is a 1 chain structure of [[Antifreeze protein]] with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MSI OCA].
		+
		+	==See Also==
		+	*[[Antifreeze protein\|Antifreeze protein]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9466928~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009466928</ref><ref group="xtra">PMID:011742118</ref><references group="xtra"/>
-	[[Category: ~~Macrozoarces~~ americanus]]	+	[[Category: Zoarces americanus]]
	[[Category: Davies, P L.]]		[[Category: Davies, P L.]]
	[[Category: Deluca, C I.]]		[[Category: Deluca, C I.]]
	[[Category: Jia, Z.]]		[[Category: Jia, Z.]]
	[[Category: Ye, Q.]]		[[Category: Ye, Q.]]
		+	[[Category: Antifreeze]]
	[[Category: Antifreeze polypeptide]]		[[Category: Antifreeze polypeptide]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
Line 34:		Line 26:
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Thermal hysteresis protein]]		[[Category: Thermal hysteresis protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:46:38 2009''