3mxb - Revision history

OCA at 10:27, 21 February 2024

2024-02-21T10:27:00Z

←Older revision		Revision as of 10:27, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3mxb' size='340' side='right'caption='[[3mxb]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3mxb' size='340' side='right'caption='[[3mxb]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3mxb]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chlre Chlre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3MXB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3mxb]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MXB FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[3mxa\|3mxa]]~~, ~~[[3mx9\|3mx9]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3mxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mxb OCA], [~~http~~://pdbe.org/3mxb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3mxb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3mxb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3mxb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mxb OCA], [https://pdbe.org/3mxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mxb RCSB], [https://www.ebi.ac.uk/pdbsum/3mxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mxb ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 18:		Line 18:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mxb ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mxb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1 gene. Mutations in RAG1 produce severe combined immunodeficiency (SCID), a monogenic disease leading to defective immune response in the individuals, leaving them vulnerable to infectious diseases. The structures of two engineered heterodimeric variants and one single-chain variant of I-CreI, in complex with a 24-bp oligonucleotide of the human RAG1 gene sequence, show how the DNA binding is achieved through interactions in the major groove. In addition, the introduction of the G19S mutation in the neighborhood of the catalytic site lowers the reaction energy barrier for DNA cleavage without compromising DNA recognition. Gene-targeting experiments in human cell lines show that the designed single-chain molecule preserves its in vivo activity with higher specificity, further enhanced by the G19S mutation. This is the first time that an engineered meganuclease variant targets the human RAG1 locus by stimulating homologous recombination in human cell lines up to 265 bp away from the cleavage site. Our analysis illustrates the key features for a la carte procedure in protein-DNA recognition design, opening new possibilities for SCID patients whose illness can be treated ex vivo.
-
-	Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.,Munoz IG, Prieto J, Subramanian S, Coloma J, Redondo P, Villate M, Merino N, Marenchino M, D'Abramo M, Gervasio FL, Grizot S, Daboussi F, Smith J, Chion-Sotinel I, Paques F, Duchateau P, Alibes A, Stricher F, Serrano L, Blanco FJ, Montoya G Nucleic Acids Res. 2010 Sep 16. PMID:20846960<ref>PMID:20846960</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3mxb" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlre~~]]	+	[[Category: Chlamydomonas reinhardtii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Coloma, J]]	+	[[Category: Coloma J]]
-	[[Category: Montoya, G]]	+	[[Category: Montoya G]]
-	[[Category: Munoz~~, I G~~]]	+	[[Category: Munoz IG]]
-	[[Category: Prieto, J]]	+	[[Category: Prieto J]]
-	[[Category: Subramanian, S]]	+	[[Category: Subramanian S]]
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Protein-dna complex~~]]	+

OCA at 08:59, 21 October 2020

2020-10-21T08:59:58Z

←Older revision		Revision as of 08:59, 21 October 2020
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	==Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus==		==Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus==
-	<StructureSection load='3mxb' size='340' side='right' caption='[[3mxb]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3mxb' size='340' side='right'caption='[[3mxb]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3mxb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3MXB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3mxb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3MXB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mxa\|3mxa]], [[3mx9\|3mx9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mxa\|3mxa]], [[3mx9\|3mx9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3mxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mxb OCA], [http://pdbe.org/3mxb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mxb RCSB], [http://www.ebi.ac.uk/pdbsum/3mxb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mxb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3mxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mxb OCA], [http://pdbe.org/3mxb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mxb RCSB], [http://www.ebi.ac.uk/pdbsum/3mxb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mxb ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Chlre]]		[[Category: Chlre]]
		+	[[Category: Large Structures]]
	[[Category: Coloma, J]]		[[Category: Coloma, J]]
	[[Category: Montoya, G]]		[[Category: Montoya, G]]

OCA at 13:41, 16 January 2019

2019-01-16T13:41:52Z

←Older revision		Revision as of 13:41, 16 January 2019
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mx/3mxb_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mx/3mxb_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:24, 5 August 2016

2016-08-05T23:24:22Z

←Older revision		Revision as of 23:24, 5 August 2016
Line 1:		Line 1:
		+
	==Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus==		==Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus==
	<StructureSection load='3mxb' size='340' side='right' caption='[[3mxb]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3mxb' size='340' side='right' caption='[[3mxb]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3mxb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chlamydomonas_reinhardtii Chlamydomonas reinhardtii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MXB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3mxb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MXB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mxa\|3mxa]], [[3mx9\|3mx9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mxa\|3mxa]], [[3mx9\|3mx9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mxb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mxb RCSB], [http://www.ebi.ac.uk/pdbsum/3mxb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mxb OCA], [http://pdbe.org/3mxb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mxb RCSB], [http://www.ebi.ac.uk/pdbsum/3mxb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mxb ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mxb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3mxb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlamydomonas reinhardtii~~]]	+	[[Category: Chlre]]
	[[Category: Coloma, J]]		[[Category: Coloma, J]]
	[[Category: Montoya, G]]		[[Category: Montoya, G]]

OCA at 17:58, 18 December 2014

2014-12-18T17:58:37Z

←Older revision		Revision as of 17:58, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3mxb\| PDB=3mxb \| SCENE= }}~~	+	==Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus==
-	===Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus===	+	<StructureSection load='3mxb' size='340' side='right' caption='[[3mxb]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20846960}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3mxb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MXB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mxa\|3mxa]], [[3mx9\|3mx9]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mxb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mxb RCSB], [http://www.ebi.ac.uk/pdbsum/3mxb PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mx/3mxb_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1 gene. Mutations in RAG1 produce severe combined immunodeficiency (SCID), a monogenic disease leading to defective immune response in the individuals, leaving them vulnerable to infectious diseases. The structures of two engineered heterodimeric variants and one single-chain variant of I-CreI, in complex with a 24-bp oligonucleotide of the human RAG1 gene sequence, show how the DNA binding is achieved through interactions in the major groove. In addition, the introduction of the G19S mutation in the neighborhood of the catalytic site lowers the reaction energy barrier for DNA cleavage without compromising DNA recognition. Gene-targeting experiments in human cell lines show that the designed single-chain molecule preserves its in vivo activity with higher specificity, further enhanced by the G19S mutation. This is the first time that an engineered meganuclease variant targets the human RAG1 locus by stimulating homologous recombination in human cell lines up to 265 bp away from the cleavage site. Our analysis illustrates the key features for a la carte procedure in protein-DNA recognition design, opening new possibilities for SCID patients whose illness can be treated ex vivo.

-	~~==About this Structure==~~	+	Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.,Munoz IG, Prieto J, Subramanian S, Coloma J, Redondo P, Villate M, Merino N, Marenchino M, D'Abramo M, Gervasio FL, Grizot S, Daboussi F, Smith J, Chion-Sotinel I, Paques F, Duchateau P, Alibes A, Stricher F, Serrano L, Blanco FJ, Montoya G Nucleic Acids Res. 2010 Sep 16. PMID:20846960<ref>PMID:20846960</ref>
-	~~[[3mxb]] is a 8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:020846960</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
-	[[Category: Coloma, J.]]	+	[[Category: Coloma, J]]
-	[[Category: Montoya, G.]]	+	[[Category: Montoya, G]]
-	[[Category: Munoz, I G.]]	+	[[Category: Munoz, I G]]
-	[[Category: Prieto, J.]]	+	[[Category: Prieto, J]]
-	[[Category: Subramanian, S.]]	+	[[Category: Subramanian, S]]
	[[Category: Hydrolase-dna complex]]		[[Category: Hydrolase-dna complex]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]

OCA at 21:09, 17 April 2013

2013-04-17T21:09:22Z

←Older revision		Revision as of 21:09, 17 April 2013
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-	[[Image:3mxb.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3mxb", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3mxb\| PDB=3mxb \| SCENE= }}		{{STRUCTURE_3mxb\| PDB=3mxb \| SCENE= }}
-
	===Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus===		===Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20846960}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20846960 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20846960}}		{{ABSTRACT_PUBMED_20846960}}

Line 22:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20846960~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020846960</ref><references group="xtra"/><references/>
	[[Category: Chlamydomonas reinhardtii]]		[[Category: Chlamydomonas reinhardtii]]
	[[Category: Coloma, J.]]		[[Category: Coloma, J.]]
Line 29:		Line 14:
	[[Category: Prieto, J.]]		[[Category: Prieto, J.]]
	[[Category: Subramanian, S.]]		[[Category: Subramanian, S.]]
		+	[[Category: Hydrolase-dna complex]]
		+	[[Category: Protein-dna complex]]

OCA: Protected "3mxb" [edit=sysop:move=sysop]

2011-02-09T05:26:38Z

Protected "3mxb" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:26, 9 February 2011

OCA at 05:26, 9 February 2011

2011-02-09T05:26:37Z

←Older revision		Revision as of 05:26, 9 February 2011
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3mxb.png\|left\|200px]]
-	[[Image:3mxb.~~jpg~~\|left\|200px]]	+

	<!--		<!--
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	==About this Structure==		==About this Structure==
-	~~3MXB~~ is a 8 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA].	+	[[3mxb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA].

	==Reference==		==Reference==
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	[[Category: Prieto, J.]]		[[Category: Prieto, J.]]
	[[Category: Subramanian, S.]]		[[Category: Subramanian, S.]]
-	[[Category: Hydrolase-dna complex]]
-	[[Category: Protein-dna complex]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 06:05:58 2010''

OCA at 02:56, 6 October 2010

2010-10-06T02:56:27Z

←Older revision		Revision as of 02:56, 6 October 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3mxb.jpg\|left\|200px]]

-	The ~~entry 3mxb~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3mxb", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3mxb\| PDB=3mxb \| SCENE= }}

-	~~Authors: Munoz, I.G., Prieto, J., Subramanian, S., Coloma, J., Montoya, G.~~	+	===Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus===

-	Description: Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug~~ ~~4 08~~:46:39 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20846960}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20846960 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20846960}}
		+
		+	==About this Structure==
		+	3MXB is a 8 chains structure with sequences from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MXB OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20846960</ref><references group="xtra"/>
		+	[[Category: Chlamydomonas reinhardtii]]
		+	[[Category: Coloma, J.]]
		+	[[Category: Montoya, G.]]
		+	[[Category: Munoz, I G.]]
		+	[[Category: Prieto, J.]]
		+	[[Category: Subramanian, S.]]
		+	[[Category: Hydrolase-dna complex]]
		+	[[Category: Protein-dna complex]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 06:05:58 2010''

OCA at 04:39, 4 August 2010

2010-08-04T04:39:40Z

←Older revision		Revision as of 04:39, 4 August 2010
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3mxb is ON HOLD	+	The entry 3mxb is ON HOLD until Paper Publication

	Authors: Munoz, I.G., Prieto, J., Subramanian, S., Coloma, J., Montoya, G.		Authors: Munoz, I.G., Prieto, J., Subramanian, S., Coloma, J., Montoya, G.
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	Description: Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus		Description: Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu May 20 09~~:19:09 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 4 08:46:39 2010''