3n4i - Revision history

OCA at 09:08, 6 September 2023

2023-09-06T09:08:32Z

←Older revision		Revision as of 09:08, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3n4i' size='340' side='right'caption='[[3n4i]], [[Resolution\|resolution]] 1.56Å' scene=''>		<StructureSection load='3n4i' size='340' side='right'caption='[[3n4i]], [[Resolution\|resolution]] 1.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n4i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus~~ pneumoniae~~" (schroeter 1886) flugge 1886~~] and [https://en.wikipedia.org/wiki/~~As_4.1611 As 4.1611~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N4I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n4i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N4I FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2g2u\|2g2u]], [[1jtg\|1jtg]], [[3e2l\|3e2l]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.56Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">bla, bla SHV1, RSMK03643, RSMK03800, shv1 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886]), bli ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n4i OCA], [https://pdbe.org/3n4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n4i RCSB], [https://www.ebi.ac.uk/pdbsum/3n4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n4i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n4i OCA], [https://pdbe.org/3n4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n4i RCSB], [https://www.ebi.ac.uk/pdbsum/3n4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n4i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~BLIP_STRCL BLIP_STRCL~~]~~] Inhibits a wide variety of beta lactamases.~~	+	[https://www.uniprot.org/uniprot/BLA1_KLEPN BLA1_KLEPN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~As 4 1611]]~~	+	[[Category: Klebsiella pneumoniae]]
-	~~[[Category: Beta-lactamase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bonomo~~, R A~~]]	+	[[Category: Streptomyces clavuligerus]]
-	[[Category: Ghosh, P]]	+	[[Category: Bonomo RA]]
-	[[Category: Handel~~, T M~~]]	+	[[Category: Ghosh P]]
-	[[Category: Hanes~~, M S~~]]	+	[[Category: Handel TM]]
-	[[Category: Kirsch~~, J F~~]]	+	[[Category: Hanes MS]]
-	[[Category: McNamara, C]]	+	[[Category: Kirsch JF]]
-	[[Category: Reynolds~~, K A]]~~	+	[[Category: McNamara C]]
-	~~[[Category: Blip]]~~	+	[[Category: Reynolds KA]]
-	~~[[Category: Hydrolase - hydrolase inhibitor complex]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Shv-1~~]]	+

OCA at 06:55, 12 May 2022

2022-05-12T06:55:06Z

←Older revision		Revision as of 06:55, 12 May 2022
Line 1:		Line 1:

	==Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex==		==Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex==
-	<StructureSection load='3n4i' size='340' side='right' caption='[[3n4i]], [[Resolution\|resolution]] 1.56Å' scene=''>	+	<StructureSection load='3n4i' size='340' side='right'caption='[[3n4i]], [[Resolution\|resolution]] 1.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n4i]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886] and [~~http~~://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3N4I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n4i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886] and [https://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N4I FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g2u\|2g2u]], [[1jtg\|1jtg]], [[3e2l\|3e2l]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2g2u\|2g2u]], [[1jtg\|1jtg]], [[3e2l\|3e2l]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, bla SHV1, RSMK03643, RSMK03800, shv1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886]), bli ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, bla SHV1, RSMK03643, RSMK03800, shv1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886]), bli ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n4i OCA], [~~http~~://pdbe.org/3n4i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3n4i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3n4i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3n4i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n4i OCA], [https://pdbe.org/3n4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n4i RCSB], [https://www.ebi.ac.uk/pdbsum/3n4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n4i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BLIP_STRCL BLIP_STRCL]] Inhibits a wide variety of beta lactamases.	+	[[https://www.uniprot.org/uniprot/BLIP_STRCL BLIP_STRCL]] Inhibits a wide variety of beta lactamases.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3n4i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3n4i" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
		+	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 30:
	[[Category: As 4 1611]]		[[Category: As 4 1611]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Bonomo, R A]]		[[Category: Bonomo, R A]]
	[[Category: Ghosh, P]]		[[Category: Ghosh, P]]

OCA at 08:23, 8 November 2017

2017-11-08T08:23:23Z

←Older revision		Revision as of 08:23, 8 November 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3n4i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3n4i" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]
-	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:13, 5 August 2016

2016-08-05T08:13:55Z

←Older revision		Revision as of 08:13, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex==		==Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex==
	<StructureSection load='3n4i' size='340' side='right' caption='[[3n4i]], [[Resolution\|resolution]] 1.56Å' scene=''>		<StructureSection load='3n4i' size='340' side='right' caption='[[3n4i]], [[Resolution\|resolution]] 1.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n4i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Klebsiella_pneumoniae Klebsiella~~ pneumoniae] and [http://en.wikipedia.org/wiki/~~Streptomyces_clavuligerus Streptomyces clavuligerus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N4I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n4i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886] and [http://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N4I FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g2u\|2g2u]], [[1jtg\|1jtg]], [[3e2l\|3e2l]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g2u\|2g2u]], [[1jtg\|1jtg]], [[3e2l\|3e2l]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, bla SHV1, RSMK03643, RSMK03800, shv1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 ~~Klebsiella~~ pneumoniae]), bli ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 ~~Streptomyces clavuligerus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, bla SHV1, RSMK03643, RSMK03800, shv1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886]), bli ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 AS 4.1611])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n4i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n4i RCSB], [http://www.ebi.ac.uk/pdbsum/3n4i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n4i OCA], [http://pdbe.org/3n4i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n4i RCSB], [http://www.ebi.ac.uk/pdbsum/3n4i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n4i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3n4i" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1611]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
-	[[Category: Klebsiella pneumoniae]]
-	[[Category: Streptomyces clavuligerus]]
	[[Category: Bonomo, R A]]		[[Category: Bonomo, R A]]
	[[Category: Ghosh, P]]		[[Category: Ghosh, P]]

OCA at 00:36, 25 December 2014

2014-12-25T00:36:08Z

←Older revision		Revision as of 00:36, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n4i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n4i RCSB], [http://www.ebi.ac.uk/pdbsum/3n4i PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n4i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n4i RCSB], [http://www.ebi.ac.uk/pdbsum/3n4i PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BLIP_STRCL BLIP_STRCL]] Inhibits a wide variety of beta lactamases.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 14:57, 18 December 2014

2014-12-18T14:57:25Z

←Older revision		Revision as of 14:57, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3n4i\| PDB=3n4i \| SCENE= }}~~	+	==Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex==
-	===Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex===	+	<StructureSection load='3n4i' size='340' side='right' caption='[[3n4i]], [[Resolution\|resolution]] 1.56Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21294157}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3n4i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N4I FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g2u\|2g2u]], [[1jtg\|1jtg]], [[3e2l\|3e2l]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, bla SHV1, RSMK03643, RSMK03800, shv1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae]), bli ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 Streptomyces clavuligerus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n4i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n4i RCSB], [http://www.ebi.ac.uk/pdbsum/3n4i PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Establishing a quantitative understanding of the determinants of affinity in protein-protein interactions remains challenging. For example, TEM-1/beta-lactamase inhibitor protein (BLIP) and SHV-1/BLIP are homologous beta-lactamase/beta-lactamase inhibitor protein complexes with disparate K(d) values (3 nM and 2 muM, respectively), and a single substitution, D104E in SHV-1, results in a 1000-fold enhancement in binding affinity. In TEM-1, E104 participates in a salt bridge with BLIP K74, whereas the corresponding SHV-1 D104 does not in the wild type SHV-1/BLIP co-structure. Here, we present a 1.6 A crystal structure of the SHV-1 D104E/BLIP complex that demonstrates that this point mutation restores this salt bridge. Additionally, mutation of a neighboring residue, BLIP E73M, results in salt bridge formation between SHV-1 D104 and BLIP K74 and a 400-fold increase in binding affinity. To understand how this salt bridge contributes to complex affinity, the cooperativity between the E/K or D/K salt bridge pair and a neighboring hot spot residue (BLIP F142) was investigated using double mutant cycle analyses in the background of the E73M mutation. We find that BLIP F142 cooperatively stabilizes both interactions, illustrating how a single mutation at a hot spot position can drive large perturbations in interface stability and specificity through a cooperative interaction network. Proteins 2011. (c) 2011 Wiley-Liss, Inc.

-	~~==Function==~~	+	Specificity and cooperativity at beta-lactamase position 104 in TEM-1/BLIP and SHV-1/BLIP interactions.,Hanes MS, Reynolds KA, McNamara C, Ghosh P, Bonomo RA, Kirsch JF, Handel TM Proteins. 2010 Dec 13. doi: 10.1002/prot.22961. PMID:21294157<ref>PMID:21294157</ref>
-	~~[[http:~~//~~www~~.~~uniprot~~.~~org~~/~~uniprot/BLIP_STRCL BLIP_STRCL]] Inhibits a wide variety of beta lactamases~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3n4i]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [http://en~~.~~wikipedia.org~~/~~wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:021294157</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Beta-lactamase\|Beta-lactamase]]
		+	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
	[[Category: Streptomyces clavuligerus]]		[[Category: Streptomyces clavuligerus]]
-	[[Category: Bonomo, R A.]]	+	[[Category: Bonomo, R A]]
-	[[Category: Ghosh, P.]]	+	[[Category: Ghosh, P]]
-	[[Category: Handel, T M.]]	+	[[Category: Handel, T M]]
-	[[Category: Hanes, M S.]]	+	[[Category: Hanes, M S]]
-	[[Category: Kirsch, J F.]]	+	[[Category: Kirsch, J F]]
-	[[Category: McNamara, C.]]	+	[[Category: McNamara, C]]
-	[[Category: Reynolds, K A~~.]]~~	+	[[Category: Reynolds, K A]]
-	~~[[Category: Beta-lactamase~~]]	+
	[[Category: Blip]]		[[Category: Blip]]
	[[Category: Hydrolase - hydrolase inhibitor complex]]		[[Category: Hydrolase - hydrolase inhibitor complex]]

OCA at 07:35, 20 June 2013

2013-06-20T07:35:12Z

←Older revision		Revision as of 07:35, 20 June 2013
Line 1:		Line 1:
-	[[Image:3n4i.png\|left\|200px]]
-
	{{STRUCTURE_3n4i\| PDB=3n4i \| SCENE= }}		{{STRUCTURE_3n4i\| PDB=3n4i \| SCENE= }}
-
	===Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex===		===Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex===
-
	{{ABSTRACT_PUBMED_21294157}}		{{ABSTRACT_PUBMED_21294157}}
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/BLIP_STRCL BLIP_STRCL]] Inhibits a wide variety of beta lactamases.

	==About this Structure==		==About this Structure==
	[[3n4i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA].		[[3n4i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA].
-
-	==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]
-	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:021294157</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021294157</ref><references group="xtra"/><references/>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
Line 27:		Line 22:
	[[Category: Reynolds, K A.]]		[[Category: Reynolds, K A.]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
-	[[Category: Beta-lactamase inhibitor protein]]
	[[Category: Blip]]		[[Category: Blip]]
-	[[Category: Hydrolase]]
	[[Category: Hydrolase - hydrolase inhibitor complex]]		[[Category: Hydrolase - hydrolase inhibitor complex]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Protein-protein complex]]		[[Category: Protein-protein complex]]
	[[Category: Shv-1]]		[[Category: Shv-1]]

OCA at 10:44, 20 October 2012

2012-10-20T10:44:15Z

←Older revision		Revision as of 10:44, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3n4i]] is a 2 chain structure ~~of [[Beta-lactamase]] and [[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]~~ with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA].	+	[[3n4i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA].

	==See Also==		==See Also==

OCA at 03:06, 26 July 2012

2012-07-26T03:06:12Z

←Older revision		Revision as of 03:06, 26 July 2012
Line 1:		Line 1:
-	[[Image:3n4i.~~jpg~~\|left\|200px]]	+	[[Image:3n4i.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3n4i", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3n4i\| PDB=3n4i \| SCENE= }}		{{STRUCTURE_3n4i\| PDB=3n4i \| SCENE= }}

	===Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex===		===Crystal structure of the SHV-1 D104E beta-lactamase/beta-lactamase inhibitor protein (BLIP) complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21294157}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21294157 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21294157}}		{{ABSTRACT_PUBMED_21294157}}

	==About this Structure==		==About this Structure==
-	[[3n4i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA].	+	[[3n4i]] is a 2 chain structure of [[Beta-lactamase]] and [[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]] with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N4I OCA].
		+
		+	==See Also==
		+	*[[Beta-lactamase\|Beta-lactamase]]
		+	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21294157~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021294157</ref><references group="xtra"/>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Klebsiella pneumoniae]]		[[Category: Klebsiella pneumoniae]]
Line 33:		Line 26:
	[[Category: McNamara, C.]]		[[Category: McNamara, C.]]
	[[Category: Reynolds, K A.]]		[[Category: Reynolds, K A.]]
		+	[[Category: Beta-lactamase]]
		+	[[Category: Beta-lactamase inhibitor protein]]
		+	[[Category: Blip]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase - hydrolase inhibitor complex]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]
		+	[[Category: Protein-protein complex]]
		+	[[Category: Shv-1]]

OCA: Protected "3n4i" [edit=sysop:move=sysop]

2011-03-09T06:27:31Z

Protected "3n4i" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:27, 9 March 2011