3nc1 - Revision history

OCA at 07:57, 12 July 2023

2023-07-12T07:57:53Z

←Older revision		Revision as of 07:57, 12 July 2023
Line 3:		Line 3:
	<StructureSection load='3nc1' size='340' side='right'caption='[[3nc1]], [[Resolution\|resolution]] 3.35Å' scene=''>		<StructureSection load='3nc1' size='340' side='right'caption='[[3nc1]], [[Resolution\|resolution]] 3.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3nc1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~] and [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3nc1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NC1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GTP:GUANOSINE~~-~~5-TRIPHOSPHATE'>GTP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.35Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ARA24, OK/SW~~-~~cl.81~~, ~~RAN, RAN (GeneID: 5901) ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN]), Crm1, Xpo1, Xpo1 (GeneID: 103573) ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc1 OCA], [https://pdbe.org/3nc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nc1 RCSB], [https://www.ebi.ac.uk/pdbsum/3nc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nc1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc1 OCA], [https://pdbe.org/3nc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nc1 RCSB], [https://www.ebi.ac.uk/pdbsum/3nc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nc1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> [[https://www.uniprot.org/uniprot/XPO1_MOUSE XPO1_MOUSE]] Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).<ref>PMID:20921223</ref>	+	[https://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Corsini, L]]	+	[[Category: Corsini L]]
-	[[Category: Deichsel, D]]	+	[[Category: Deichsel D]]
-	[[Category: Ficner, R]]	+	[[Category: Ficner R]]
-	[[Category: Gorlich, D]]	+	[[Category: Gorlich D]]
-	[[Category: Guttler, T]]	+	[[Category: Guttler T]]
-	[[Category: Madl, T]]	+	[[Category: Madl T]]
-	[[Category: Monecke, T]]	+	[[Category: Monecke T]]
-	[[Category: Neumann, P]]	+	[[Category: Neumann P]]
-	[[Category: Sattler, M]]	+	[[Category: Sattler M]]
-	~~[[Category: Gtp-binding protein-transport protein complex]]~~	+
-	~~[[Category: Protein transport~~]]	+

OCA at 05:43, 6 October 2021

2021-10-06T05:43:30Z

←Older revision		Revision as of 05:43, 6 October 2021
Line 1:		Line 1:

	==Crystal structure of the CRM1-RanGTP complex==		==Crystal structure of the CRM1-RanGTP complex==
-	<StructureSection load='3nc1' size='340' side='right' caption='[[3nc1]], [[Resolution\|resolution]] 3.35Å' scene=''>	+	<StructureSection load='3nc1' size='340' side='right'caption='[[3nc1]], [[Resolution\|resolution]] 3.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3nc1]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human] and [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3NC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3nc1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human] and [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NC1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARA24, OK/SW-cl.81, RAN, RAN (GeneID: 5901) ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Crm1, Xpo1, Xpo1 (GeneID: 103573) ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARA24, OK/SW-cl.81, RAN, RAN (GeneID: 5901) ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Crm1, Xpo1, Xpo1 (GeneID: 103573) ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc1 OCA], [~~http~~://pdbe.org/3nc1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3nc1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3nc1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3nc1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc1 OCA], [https://pdbe.org/3nc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nc1 RCSB], [https://www.ebi.ac.uk/pdbsum/3nc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nc1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> [[~~http~~://www.uniprot.org/uniprot/XPO1_MOUSE XPO1_MOUSE]] Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).<ref>PMID:20921223</ref>	+	[[https://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> [[https://www.uniprot.org/uniprot/XPO1_MOUSE XPO1_MOUSE]] Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).<ref>PMID:20921223</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Exportin\|Exportin]]	+	*[[Exportin 3D structures\|Exportin 3D structures]]
-	*[[GTP-binding protein\|GTP-binding protein]]	+	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Corsini, L]]		[[Category: Corsini, L]]

OCA at 09:34, 4 August 2016

2016-08-04T09:34:52Z

←Older revision		Revision as of 09:34, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the CRM1-RanGTP complex==		==Crystal structure of the CRM1-RanGTP complex==
	<StructureSection load='3nc1' size='340' side='right' caption='[[3nc1]], [[Resolution\|resolution]] 3.35Å' scene=''>		<StructureSection load='3nc1' size='340' side='right' caption='[[3nc1]], [[Resolution\|resolution]] 3.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3nc1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~] and [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3nc1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NC1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARA24, OK/SW-cl.81, RAN, RAN (GeneID: 5901) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), Crm1, Xpo1, Xpo1 (GeneID: 103573) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARA24, OK/SW-cl.81, RAN, RAN (GeneID: 5901) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Crm1, Xpo1, Xpo1 (GeneID: 103573) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/3nc1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc1 OCA], [http://pdbe.org/3nc1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/3nc1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nc1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3nc1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Corsini, L]]		[[Category: Corsini, L]]
	[[Category: Deichsel, D]]		[[Category: Deichsel, D]]

OCA at 08:20, 24 December 2014

2014-12-24T08:20:31Z

←Older revision		Revision as of 08:20, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/3nc1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/3nc1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> [[http://www.uniprot.org/uniprot/XPO1_MOUSE XPO1_MOUSE]] Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).<ref>PMID:20921223</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:28, 18 December 2014

2014-12-18T16:28:39Z

←Older revision		Revision as of 16:28, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3nc1\| PDB~~=3nc1 \| ~~SCENE~~= }}	+	==Crystal structure of the CRM1-RanGTP complex==
-	===~~Crystal~~ structure of the ~~CRM1~~-~~RanGTP complex~~===	+	<StructureSection load='3nc1' size='340' side='right' caption='[[3nc1]], [[Resolution\|resolution]] 3.35Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20972448}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3nc1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NC1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARA24, OK/SW-cl.81, RAN, RAN (GeneID: 5901) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), Crm1, Xpo1, Xpo1 (GeneID: 103573) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/3nc1 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Classic nuclear export signals (NESs) confer CRM1-dependent nuclear export. Here we present crystal structures of the RanGTP-CRM1 complex alone and bound to the prototypic PKI or HIV-1 Rev NESs. These NESs differ markedly in the spacing of their key hydrophobic (Phi) residues, yet CRM1 recognizes them with the same rigid set of five Phi pockets. The different Phi spacings are compensated for by different conformations of the bound NESs: in the case of PKI, an alpha-helical conformation, and in the case of Rev, an extended conformation with a critical proline docking into a Phi pocket. NMR analyses of CRM1-bound and CRM1-free PKI NES suggest that CRM1 selects NES conformers that pre-exist in solution. Our data lead to a new structure-based NES consensus, and explain why NESs differ in their affinities for CRM1 and why supraphysiological NESs bind the exportin so tightly.

-	~~==Function==~~	+	NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1.,Guttler T, Madl T, Neumann P, Deichsel D, Corsini L, Monecke T, Ficner R, Sattler M, Gorlich D Nat Struct Mol Biol. 2010 Oct 24. PMID:20972448<ref>PMID:20972448</ref>
-	[[http://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by ~~serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator~~ of ~~the kinase activity of VRK1~~ and ~~VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR~~-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> [[http://www.uniprot.org/uniprot/XPO1_MOUSE XPO1_MOUSE]] Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound ~~form. Docking of this complex~~ to ~~the nuclear pore complex (NPC) is mediated through binding to nucleoporins~~. ~~Upon transit of a nuclear export complex into the cytoplasm~~, ~~disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1~~, ~~respectively) cause release of the cargo from the export receptor~~. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).<ref>PMID:~~20921223~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3nc1]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens] and [http://en~~.~~wikipedia.org~~/~~wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:020972448</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Exportin\|Exportin]]
		+	*[[GTP-binding protein\|GTP-binding protein]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Corsini, L.]]	+	[[Category: Corsini, L]]
-	[[Category: Deichsel, D.]]	+	[[Category: Deichsel, D]]
-	[[Category: Ficner, R.]]	+	[[Category: Ficner, R]]
-	[[Category: Gorlich, D.]]	+	[[Category: Gorlich, D]]
-	[[Category: Guttler, T.]]	+	[[Category: Guttler, T]]
-	[[Category: Madl, T.]]	+	[[Category: Madl, T]]
-	[[Category: Monecke, T.]]	+	[[Category: Monecke, T]]
-	[[Category: Neumann, P.]]	+	[[Category: Neumann, P]]
-	[[Category: Sattler, M.]]	+	[[Category: Sattler, M]]
	[[Category: Gtp-binding protein-transport protein complex]]		[[Category: Gtp-binding protein-transport protein complex]]
	[[Category: Protein transport]]		[[Category: Protein transport]]

OCA at 12:04, 24 April 2013

2013-04-24T12:04:52Z

←Older revision		Revision as of 12:04, 24 April 2013
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-	[[Image:3nc1.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3nc1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3nc1\| PDB=3nc1 \| SCENE= }}		{{STRUCTURE_3nc1\| PDB=3nc1 \| SCENE= }}
-
	===Crystal structure of the CRM1-RanGTP complex===		===Crystal structure of the CRM1-RanGTP complex===
		+	{{ABSTRACT_PUBMED_20972448}}

-		+	==Function==
-	<!--	+	[[http://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> [[http://www.uniprot.org/uniprot/XPO1_MOUSE XPO1_MOUSE]] Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).<ref>PMID:20921223</ref>
-	The ~~line below this paragraph, {{ABSTRACT_PUBMED_20972448}}, adds~~ the ~~Publication Abstract~~ to the ~~page~~	+
-	(as ~~it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~), ~~where 20972448~~ is the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_20972448}}~~	+

	==About this Structure==		==About this Structure==
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20972448~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020972448</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
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	[[Category: Neumann, P.]]		[[Category: Neumann, P.]]
	[[Category: Sattler, M.]]		[[Category: Sattler, M.]]
		+	[[Category: Gtp-binding protein-transport protein complex]]
		+	[[Category: Protein transport]]

OCA: Protected "3nc1" [edit=sysop:move=sysop]

2011-01-05T15:41:08Z

Protected "3nc1" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:41, 5 January 2011

OCA at 15:41, 5 January 2011

2011-01-05T15:41:07Z

←Older revision		Revision as of 15:41, 5 January 2011
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-	{{Seed}}
	[[Image:3nc1.png\|left\|200px]]		[[Image:3nc1.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~3NC1~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA].	+	[[3nc1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA].

	==Reference==		==Reference==
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	[[Category: Neumann, P.]]		[[Category: Neumann, P.]]
	[[Category: Sattler, M.]]		[[Category: Sattler, M.]]
-	[[Category: Gtp-binding protein-transport protein complex]]
-	[[Category: Protein transport]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 15 08:10:31 2010''

OCA at 04:58, 15 December 2010

2010-12-15T04:58:12Z

←Older revision		Revision as of 04:58, 15 December 2010
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	{{STRUCTURE_3nc1\| PDB=3nc1 \| SCENE= }}		{{STRUCTURE_3nc1\| PDB=3nc1 \| SCENE= }}

-	===~~Protein~~ complex===	+	===Crystal structure of the CRM1-RanGTP complex===


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	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
		+	[[Category: Corsini, L.]]
		+	[[Category: Deichsel, D.]]
		+	[[Category: Ficner, R.]]
		+	[[Category: Gorlich, D.]]
		+	[[Category: Guttler, T.]]
		+	[[Category: Madl, T.]]
		+	[[Category: Monecke, T.]]
	[[Category: Neumann, P.]]		[[Category: Neumann, P.]]
		+	[[Category: Sattler, M.]]
	[[Category: Gtp-binding protein-transport protein complex]]		[[Category: Gtp-binding protein-transport protein complex]]
	[[Category: Protein transport]]		[[Category: Protein transport]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov 3 11~~:21:44 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 15 08:10:31 2010''

OCA at 08:11, 3 November 2010

2010-11-03T08:11:06Z

←Older revision		Revision as of 08:11, 3 November 2010
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-	[[Image:3nc1.~~jpg~~\|left\|200px]]	+	[[Image:3nc1.png\|left\|200px]]

	<!--		<!--
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	===Protein complex===		===Protein complex===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20972448}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20972448 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20972448}}

	==About this Structure==		==About this Structure==
	3NC1 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA].		3NC1 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC1 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20972448</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
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	[[Category: Protein transport]]		[[Category: Protein transport]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Oct 27 12~~:12:59 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 3 11:21:44 2010''