3npv - Revision history

OCA at 16:49, 1 November 2023

2023-11-01T16:49:47Z

←Older revision		Revision as of 16:49, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3npv' size='340' side='right'caption='[[3npv]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='3npv' size='340' side='right'caption='[[3npv]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3npv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NPV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3npv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NPV FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[3npu\|3npu]]~~, [[~~3npw~~\|~~3npw~~]]~~, [[3npx\|3npx]], [[3nq2\|3nq2]], [[3nq8\|3nq8]], [[3nqv\|3nqv]], [[3nr0\|3nr0]]</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.48Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3npv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npv OCA], [https://pdbe.org/3npv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3npv RCSB], [https://www.ebi.ac.uk/pdbsum/3npv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3npv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3npv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npv OCA], [https://pdbe.org/3npv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3npv RCSB], [https://www.ebi.ac.uk/pdbsum/3npv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3npv ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baker, D]]	+	[[Category: Baker D]]
-	[[Category: Dym, O]]	+	[[Category: Dym O]]
-	~~[[Category: ISPC, Israel Structural Proteomics Center~~]]	+	[[Category: Khersonsky O]]
-	[[Category: Khersonsky, O]]	+	[[Category: Rothlisberge D]]
-	[[Category: Rothlisberge, D]]	+	[[Category: Tawfik DS]]
-	[[Category: Tawfik~~, D S~~]]	+	[[Category: Wollacott AM]]
-	[[Category: Wollacott~~, A M]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Tim~~]]	+

OCA at 05:26, 10 August 2022

2022-08-10T05:26:32Z

←Older revision		Revision as of 05:26, 10 August 2022
Line 3:		Line 3:
	<StructureSection load='3npv' size='340' side='right'caption='[[3npv]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='3npv' size='340' side='right'caption='[[3npv]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3npv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3NPV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3npv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NPV FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3npu\|3npu]], [[3npw\|3npw]], [[3npx\|3npx]], [[3nq2\|3nq2]], [[3nq8\|3nq8]], [[3nqv\|3nqv]], [[3nr0\|3nr0]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3npu\|3npu]], [[3npw\|3npw]], [[3npx\|3npx]], [[3nq2\|3nq2]], [[3nq8\|3nq8]], [[3nqv\|3nqv]], [[3nr0\|3nr0]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3npv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npv OCA], [~~http~~://pdbe.org/3npv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3npv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3npv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3npv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3npv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npv OCA], [https://pdbe.org/3npv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3npv RCSB], [https://www.ebi.ac.uk/pdbsum/3npv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3npv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:06, 4 November 2020

2020-11-04T08:06:18Z

←Older revision		Revision as of 08:06, 4 November 2020
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	==Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution==		==Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution==
-	<StructureSection load='3npv' size='340' side='right' caption='[[3npv]], [[Resolution\|resolution]] 1.48Å' scene=''>	+	<StructureSection load='3npv' size='340' side='right'caption='[[3npv]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3NPV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3NPV FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3npu\|3npu]], [[3npw\|3npw]], [[3npx\|3npx]], [[3nq2\|3nq2]], [[3nq8\|3nq8]], [[3nqv\|3nqv]], [[3nr0\|3nr0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3npu\|3npu]], [[3npw\|3npw]], [[3npx\|3npx]], [[3nq2\|3nq2]], [[3nq8\|3nq8]], [[3nqv\|3nqv]], [[3nr0\|3nr0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3npv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npv OCA], [http://pdbe.org/3npv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3npv RCSB], [http://www.ebi.ac.uk/pdbsum/3npv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3npv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3npv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npv OCA], [http://pdbe.org/3npv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3npv RCSB], [http://www.ebi.ac.uk/pdbsum/3npv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3npv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 18:

	==See Also==		==See Also==
-	*[[Aldolase\|Aldolase]]	+	*[[Aldolase 3D structures\|Aldolase 3D structures]]
	*[[Kemp eliminase\|Kemp eliminase]]		*[[Kemp eliminase\|Kemp eliminase]]
	== References ==		== References ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Baker, D]]		[[Category: Baker, D]]
	[[Category: Dym, O]]		[[Category: Dym, O]]

OCA at 13:19, 4 August 2016

2016-08-04T13:19:00Z

←Older revision		Revision as of 13:19, 4 August 2016
Line 1:		Line 1:
		+
	==Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution==		==Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution==
	<StructureSection load='3npv' size='340' side='right' caption='[[3npv]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='3npv' size='340' side='right' caption='[[3npv]], [[Resolution\|resolution]] 1.48Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NPV FirstGlance]. <br>		<table><tr><td colspan='2'>[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NPV FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3npu\|3npu]], [[3npw\|3npw]], [[3npx\|3npx]], [[3nq2\|3nq2]], [[3nq8\|3nq8]], [[3nqv\|3nqv]], [[3nr0\|3nr0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3npu\|3npu]], [[3npw\|3npw]], [[3npx\|3npx]], [[3nq2\|3nq2]], [[3nq8\|3nq8]], [[3nqv\|3nqv]], [[3nr0\|3nr0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3npv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3npv RCSB], [http://www.ebi.ac.uk/pdbsum/3npv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3npv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npv OCA], [http://pdbe.org/3npv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3npv RCSB], [http://www.ebi.ac.uk/pdbsum/3npv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3npv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3npv" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 22:27, 3 January 2015

2015-01-03T22:27:38Z

←Older revision		Revision as of 22:27, 3 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_3npv\| PDB=3npv \| SCENE= }}~~	+	==Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution==
-	===Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution===	+	<StructureSection load='3npv' size='340' side='right' caption='[[3npv]], [[Resolution\|resolution]] 1.48Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21277311}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NPV FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3npu\|3npu]], [[3npw\|3npw]], [[3npx\|3npx]], [[3nq2\|3nq2]], [[3nq8\|3nq8]], [[3nqv\|3nqv]], [[3nr0\|3nr0]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3npv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3npv RCSB], [http://www.ebi.ac.uk/pdbsum/3npv PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although de novo computational enzyme design has been shown to be feasible, the field is still in its infancy: the kinetic parameters of designed enzymes are still orders of magnitude lower than those of naturally occurring ones. Nonetheless, designed enzymes can be improved by directed evolution, as recently exemplified for the designed Kemp eliminase KE07. Random mutagenesis and screening resulted in variants with >200-fold higher catalytic efficiency and provided insights about features missing in the designed enzyme. Here we describe the optimization of KE70, another designed Kemp eliminase. Amino acid substitutions predicted to improve catalysis in design calculations involving extensive backbone sampling were individually tested. Those proven beneficial were combinatorially incorporated into the originally designed KE70 along with random mutations, and the resulting libraries were screened for improved eliminase activity. Nine rounds of mutation and selection resulted in >400-fold improvement in the catalytic efficiency of the original KE70 design, reflected in both higher k(cat) values and lower K(m) values, with the best variants exhibiting k(cat)/K(m) values of >5x10(4) s(-)(1) M(-1). The optimized KE70 variants were characterized structurally and biochemically, providing insights into the origins of the improvements in catalysis. Three primary contributions were identified: first, the reshaping of the active-site cavity to achieve tighter substrate binding; second, the fine-tuning of electrostatics around the catalytic His-Asp dyad; and, third, the stabilization of the active-site dyad in a conformation optimal for catalysis.

-	~~==About this Structure==~~	+	Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution.,Khersonsky O, Rothlisberger D, Wollacott AM, Murphy P, Dym O, Albeck S, Kiss G, Houk KN, Baker D, Tawfik DS J Mol Biol. 2011 Apr 1;407(3):391-412. doi: 10.1016/j.jmb.2011.01.041. Epub 2011 , Jan 28. PMID:21277311<ref>PMID:21277311</ref>
-	~~[[3npv]] is a 2 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3NPV OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Aldolase\|Aldolase]]		*[[Aldolase\|Aldolase]]
	*[[Kemp eliminase\|Kemp eliminase]]		*[[Kemp eliminase\|Kemp eliminase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021277311</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
-	[[Category: Baker, D.]]	+	[[Category: Baker, D]]
-	[[Category: Dym, O.]]	+	[[Category: Dym, O]]
-	[[Category: ISPC, Israel Structural Proteomics Center.]]	+	[[Category: ISPC, Israel Structural Proteomics Center]]
-	[[Category: Khersonsky, O.]]	+	[[Category: Khersonsky, O]]
-	[[Category: Rothlisberge, D.]]	+	[[Category: Rothlisberge, D]]
-	[[Category: Tawfik, D S.]]	+	[[Category: Tawfik, D S]]
-	[[Category: Wollacott, A M~~.]]~~	+	[[Category: Wollacott, A M]]
-	~~[[Category: ISPC]]~~	+
-	~~[[Category: Israel Structural Proteomics Center~~]]	+
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Tim]]		[[Category: Tim]]

OCA at 08:44, 19 March 2014

2014-03-19T08:44:23Z

←Older revision		Revision as of 08:44, 19 March 2014
Line 1:		Line 1:
-	[[Image:3npv.png\|left\|200px]]
-
	{{STRUCTURE_3npv\| PDB=3npv \| SCENE= }}		{{STRUCTURE_3npv\| PDB=3npv \| SCENE= }}
-
	===Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution===		===Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution===
-		+	{{ABSTRACT_PUBMED_21277311}}

	==About this Structure==		==About this Structure==
-	[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA].	+	[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA].

	==See Also==		==See Also==
	*[[Aldolase\|Aldolase]]		*[[Aldolase\|Aldolase]]
-	[[Category: ~~Escherichia~~ coli]]	+	*[[Kemp eliminase\|Kemp eliminase]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021277311</ref><references group="xtra"/><references/>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Baker, D.]]		[[Category: Baker, D.]]
	[[Category: Dym, O.]]		[[Category: Dym, O.]]

OCA at 06:35, 20 October 2012

2012-10-20T06:35:48Z

←Older revision		Revision as of 06:35, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3npv]] is a 2 chain structure ~~of [[Aldolase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA].	+	[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA].

	==See Also==		==See Also==

OCA at 13:41, 25 July 2012

2012-07-25T13:41:54Z

←Older revision		Revision as of 13:41, 25 July 2012
Line 1:		Line 1:
-	[[Image:3npv.~~jpg~~\|left\|200px]]	+	[[Image:3npv.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3npv", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3npv\| PDB=3npv \| SCENE= }}		{{STRUCTURE_3npv\| PDB=3npv \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA].	+	[[3npv]] is a 2 chain structure of [[Aldolase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA].
		+
		+	==See Also==
		+	*[[Aldolase\|Aldolase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Baker, D.]]		[[Category: Baker, D.]]
Line 22:		Line 19:
	[[Category: Tawfik, D S.]]		[[Category: Tawfik, D S.]]
	[[Category: Wollacott, A M.]]		[[Category: Wollacott, A M.]]
		+	[[Category: ISPC]]
		+	[[Category: Israel Structural Proteomics Center]]
		+	[[Category: Lyase]]
		+	[[Category: Structural genomic]]
		+	[[Category: Tim]]

OCA: Protected "3npv" [edit=sysop:move=sysop]

2011-02-09T06:10:14Z

Protected "3npv" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:10, 9 February 2011

OCA at 06:10, 9 February 2011

2011-02-09T06:10:12Z

←Older revision		Revision as of 06:10, 9 February 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3npv.jpg\|left\|200px]]

-	The ~~entry 3npv~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3npv", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3npv\| PDB=3npv \| SCENE= }}

-	~~Authors: Khersonsky, O., Rothlisberge, D., Wollacott, A.M., Dym, O., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)~~	+	===Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution===

-	Description: Optimization of the in silico designed Kemp eliminase KE70 by computational design and directed evolution

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Sep 1 09~~:34:~~12 2010''~~	+	==About this Structure==
		+	[[3npv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPV OCA].
		+	[[Category: Escherichia coli]]
		+	[[Category: Baker, D.]]
		+	[[Category: Dym, O.]]
		+	[[Category: ISPC, Israel Structural Proteomics Center.]]
		+	[[Category: Khersonsky, O.]]
		+	[[Category: Rothlisberge, D.]]
		+	[[Category: Tawfik, D S.]]
		+	[[Category: Wollacott, A M.]]