3o24 - Revision history

OCA at 09:26, 6 September 2023

2023-09-06T09:26:01Z

←Older revision		Revision as of 09:26, 6 September 2023
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	<StructureSection load='3o24' size='340' side='right'caption='[[3o24]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3o24' size='340' side='right'caption='[[3o24]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o24]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O24 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o24]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O24 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o2k\|3o2k]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o24 OCA], [https://pdbe.org/3o24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o24 RCSB], [https://www.ebi.ac.uk/pdbsum/3o24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o24 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o24 OCA], [https://pdbe.org/3o24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o24 RCSB], [https://www.ebi.ac.uk/pdbsum/3o24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o24 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~Q4G2I1_ASPFM Q4G2I1_ASPFM~~]~~] Catalyzes~~ the ~~prenylation~~ of brevianamide F. ~~Can~~ also ~~prenylate other~~ tryptophan-containing ~~cyclic dipeptides. Prenylation occurs mainly~~ at C-2, ~~but also at~~ C-3.<ref>PMID:16000710</ref> <ref>PMID:23090579</ref>	+	[https://www.uniprot.org/uniprot/FTMB_ASPFU FTMB_ASPFU] Brevianamide F prenyltransferase; part of the gene cluster that mediates the biosynthesis of fumitremorgins, indole alkaloids that carry not only intriguing chemical structures, but also interesting biological and pharmacological activities (PubMed:16000710, PubMed:23649274). The biosynthesis of fumitremorgin-type alkaloids begins by condensation of the two amino acids L-tryptophan and L-proline to brevianamide F, catalyzed by the non-ribosomal peptide synthetase ftmA (PubMed:16755625). Brevianamide F is then prenylated by the prenyltransferase ftmPT1/ftmB in the presence of dimethylallyl diphosphate, resulting in the formation of tryprostatin B (PubMed:16000710, PubMed:19113967, PubMed:21105662, PubMed:23090579). FtmPT1/ftmB shows also tryptophan aminopeptidase activity with preference for linear peptides containing a tryptophanyl moiety at the N-terminus (PubMed:18635009). The three cytochrome P450 monooxygenases, ftmP450-1/ftmC, ftmP450-2/ftmE and ftmP450-3/FtmG, are responsible for the conversion of tryprostatin B to 6-hydroxytryprostatin B, tryprostatin A to fumitremorgin C and fumitremorgin C to 12,13-dihydroxyfumitremorgin C, respectively (PubMed:19226505). The putative methyltransferase ftmMT/ftmD is expected for the conversion of 6-hydroxytryprostatin B to tryprostatin A (Probable). FtmPT2/FtmH catalyzes the prenylation of 12,13-dihydroxyfumitre-morgin C in the presence of dimethylallyl diphosphate, resulting in the formation of fumitremorgin B (PubMed:18683158). Fumitremorgin B is further converted to verruculogen by ftmOx1/ftmF via the insertion of an endoperoxide bond between the two prenyl moieties (PubMed:19763315). In some fungal species, verruculogen is further converted to fumitremorgin A, but the enzymes involved in this step have not been identified yet (Probable).<ref>PMID:16000710</ref> <ref>PMID:16755625</ref> <ref>PMID:18635009</ref> <ref>PMID:18683158</ref> <ref>PMID:19113967</ref> <ref>PMID:19226505</ref> <ref>PMID:19763315</ref> <ref>PMID:21105662</ref> <ref>PMID:23090579</ref> <ref>PMID:23649274</ref> <ref>PMID:16000710</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspergillus fumigatus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jost, M]]	+	[[Category: Jost M]]
-	[[Category: Stehle, T]]	+	[[Category: Stehle T]]
-	[[Category: Zocher~~, G E]]~~	+	[[Category: Zocher GE]]
-	~~[[Category: Brevianamide f prenyltransferase]]~~	+
-	~~[[Category: Pt barrel]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:27, 10 August 2022

2022-08-10T05:27:49Z

←Older revision		Revision as of 05:27, 10 August 2022
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	<StructureSection load='3o24' size='340' side='right'caption='[[3o24]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3o24' size='340' side='right'caption='[[3o24]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o24]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3O24 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o24]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O24 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o2k\|3o2k]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o2k\|3o2k]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3o24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o24 OCA], [~~http~~://pdbe.org/3o24 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3o24 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3o24 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3o24 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o24 OCA], [https://pdbe.org/3o24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o24 RCSB], [https://www.ebi.ac.uk/pdbsum/3o24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o24 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/Q4G2I1_ASPFM Q4G2I1_ASPFM]] Catalyzes the prenylation of brevianamide F. Can also prenylate other tryptophan-containing cyclic dipeptides. Prenylation occurs mainly at C-2, but also at C-3.<ref>PMID:16000710</ref> <ref>PMID:23090579</ref>	+	[[https://www.uniprot.org/uniprot/Q4G2I1_ASPFM Q4G2I1_ASPFM]] Catalyzes the prenylation of brevianamide F. Can also prenylate other tryptophan-containing cyclic dipeptides. Prenylation occurs mainly at C-2, but also at C-3.<ref>PMID:16000710</ref> <ref>PMID:23090579</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:09, 4 November 2020

2020-11-04T08:09:21Z

←Older revision		Revision as of 08:09, 4 November 2020
Line 1:		Line 1:

	==Crystal structure of the brevianamide F prenyltransferase FtmPT1 from Aspergillus fumigatus==		==Crystal structure of the brevianamide F prenyltransferase FtmPT1 from Aspergillus fumigatus==
-	<StructureSection load='3o24' size='340' side='right' caption='[[3o24]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3o24' size='340' side='right'caption='[[3o24]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o24]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3O24 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o24]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3O24 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o2k\|3o2k]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o2k\|3o2k]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3o24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o24 OCA], [http://pdbe.org/3o24 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o24 RCSB], [http://www.ebi.ac.uk/pdbsum/3o24 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o24 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3o24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o24 OCA], [http://pdbe.org/3o24 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o24 RCSB], [http://www.ebi.ac.uk/pdbsum/3o24 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o24 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Jost, M]]		[[Category: Jost, M]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]

OCA at 20:00, 4 August 2016

2016-08-04T20:00:09Z

←Older revision		Revision as of 20:00, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the brevianamide F prenyltransferase FtmPT1 from Aspergillus fumigatus==		==Crystal structure of the brevianamide F prenyltransferase FtmPT1 from Aspergillus fumigatus==
	<StructureSection load='3o24' size='340' side='right' caption='[[3o24]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3o24' size='340' side='right' caption='[[3o24]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o24]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O24 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o24]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O24 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o2k\|3o2k]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o2k\|3o2k]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ftmPT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 Aspergillus fumigatus])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o24 OCA], [http://pdbe.org/3o24 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o24 RCSB], [http://www.ebi.ac.uk/pdbsum/3o24 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o24 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o24 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o24 RCSB], [http://www.ebi.ac.uk/pdbsum/3o24 PDBsum]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q4G2I1_ASPFM Q4G2I1_ASPFM]] Catalyzes the prenylation of brevianamide F. Can also prenylate other tryptophan-containing cyclic dipeptides. Prenylation occurs mainly at C-2, but also at C-3.<ref>PMID:16000710</ref> <ref>PMID:23090579</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3o24" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aspergillus fumigatus]]
	[[Category: Jost, M]]		[[Category: Jost, M]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]

OCA at 07:24, 19 December 2014

2014-12-19T07:24:09Z

←Older revision		Revision as of 07:24, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3o24\| PDB=3o24 \| SCENE= }}~~	+	==Crystal structure of the brevianamide F prenyltransferase FtmPT1 from Aspergillus fumigatus==
-	===Crystal structure of the brevianamide F prenyltransferase FtmPT1 from Aspergillus fumigatus===	+	<StructureSection load='3o24' size='340' side='right' caption='[[3o24]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21105662}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3o24]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O24 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o2k\|3o2k]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ftmPT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 Aspergillus fumigatus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o24 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o24 RCSB], [http://www.ebi.ac.uk/pdbsum/3o24 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fungal indole prenyltransferases participate in a multitude of biosynthetic pathways. Their ability to prenylate diverse substrates has attracted interest for potential use in chemoenzymatic synthesis. The fungal indole prenyltransferase FtmPT1 catalyzes the prenylation of brevianamide F in the biosynthesis of fumitremorgin-type alkaloids, which show diverse pharmacological activities and are promising candidates for the development of antitumor agents. Here, we report crystal structures of unliganded Aspergillus fumigatus FtmPT1 as well as of a ternary complex of FtmPT1 bound to brevianamide F and an analogue of its isoprenoid substrate dimethylallyl diphosphate. FtmPT1 assumes a rare alpha/beta-barrel fold, consisting of 10 circularly arranged beta-strands surrounded by alpha-helices. Catalysis is performed in a hydrophobic reaction chamber at the center of the barrel. In combination with mutagenesis experiments, our analysis of the liganded and unliganded structures provides insight into the mechanism of catalysis and the determinants of regiospecificity. Sequence conservation of key features indicates that all fungal indole prenyltransferases possess similar active site architectures. However, while the dimethylallyl diphosphate binding site is strictly conserved in these enzymes, subtle changes in the reaction chamber likely allow for the accommodation of diverse aromatic substrates for prenylation. In support of this concept, we were able to redirect the regioselectivity of FtmPT1 by a single mutation of glycine 115 to threonine. This finding provides support for a potential use of fungal indole prenyltransferases as modifiable bioreactors that can be engineered to catalyze highly specific prenyl transfer reactions.

-	~~==About this~~ Structure==	+	Structure-Function Analysis of an Enzymatic Prenyl Transfer Reaction Identifies a Reaction Chamber with Modifiable Specificity.,Jost M, Zocher G, Tarcz S, Matuschek M, Xie X, Li SM, Stehle T J Am Chem Soc. 2010 Nov 24. PMID:21105662<ref>PMID:21105662</ref>
-	~~[[3o24]] is~~ a ~~1 chain structure~~ with ~~sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:021105662</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Aspergillus fumigatus]]		[[Category: Aspergillus fumigatus]]
-	[[Category: Jost, M.]]	+	[[Category: Jost, M]]
-	[[Category: Stehle, T.]]	+	[[Category: Stehle, T]]
-	[[Category: Zocher, G E.]]	+	[[Category: Zocher, G E]]
	[[Category: Brevianamide f prenyltransferase]]		[[Category: Brevianamide f prenyltransferase]]
	[[Category: Pt barrel]]		[[Category: Pt barrel]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 12:07, 24 April 2013

2013-04-24T12:07:12Z

←Older revision		Revision as of 12:07, 24 April 2013
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-	[[Image:3o24.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3o24", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3o24\| PDB=3o24 \| SCENE= }}		{{STRUCTURE_3o24\| PDB=3o24 \| SCENE= }}
-
	===Crystal structure of the brevianamide F prenyltransferase FtmPT1 from Aspergillus fumigatus===		===Crystal structure of the brevianamide F prenyltransferase FtmPT1 from Aspergillus fumigatus===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21105662}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21105662 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21105662}}		{{ABSTRACT_PUBMED_21105662}}

Line 22:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21105662~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021105662</ref><references group="xtra"/><references/>
	[[Category: Aspergillus fumigatus]]		[[Category: Aspergillus fumigatus]]
	[[Category: Jost, M.]]		[[Category: Jost, M.]]

OCA: Protected "3o24" [edit=sysop:move=sysop]

2010-12-29T17:56:53Z

Protected "3o24" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:56, 29 December 2010

OCA at 17:56, 29 December 2010

2010-12-29T17:56:52Z

←Older revision		Revision as of 17:56, 29 December 2010
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3o24.png\|left\|200px]]
-	[[Image:3o24.~~jpg~~\|left\|200px]]	+

	<!--		<!--
Line 20:		Line 19:

	==About this Structure==		==About this Structure==
-	~~3O24~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA].	+	[[3o24]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA].

	==Reference==		==Reference==
Line 31:		Line 30:
	[[Category: Pt barrel]]		[[Category: Pt barrel]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 8 11:51:15 2010''

OCA at 08:39, 8 December 2010

2010-12-08T08:39:13Z

←Older revision		Revision as of 08:39, 8 December 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3o24.jpg\|left\|200px]]

-	The ~~entry 3o24~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3o24", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3o24\| PDB=3o24 \| SCENE= }}

-	~~Authors: Jost, M., Zocher, G.E., Stehle, T.~~	+	===Crystal structure of the brevianamide F prenyltransferase FtmPT1 from Aspergillus fumigatus===

-	Description:

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug~~ 11 ~~23:36~~:51 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21105662}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21105662 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21105662}}
		+
		+	==About this Structure==
		+	3O24 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O24 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21105662</ref><references group="xtra"/>
		+	[[Category: Aspergillus fumigatus]]
		+	[[Category: Jost, M.]]
		+	[[Category: Stehle, T.]]
		+	[[Category: Zocher, G E.]]
		+	[[Category: Brevianamide f prenyltransferase]]
		+	[[Category: Pt barrel]]
		+	[[Category: Transferase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 8 11:51:15 2010''

OCA at 19:29, 11 August 2010

2010-08-11T19:29:33Z

←Older revision		Revision as of 19:29, 11 August 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3o24 is ON HOLD	+	The entry 3o24 is ON HOLD until Paper Publication

	Authors: Jost, M., Zocher, G.E., Stehle, T.		Authors: Jost, M., Zocher, G.E., Stehle, T.

-	Description: ~~Brevianamide F prenyltransferase~~	+	Description:

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug ~~4 08~~:51:47 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 11 23:36:51 2010''