3o78 - Revision history

OCA at 16:54, 1 November 2023

2023-11-01T16:54:24Z

←Older revision		Revision as of 16:54, 1 November 2023
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	<StructureSection load='3o78' size='340' side='right'caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3o78' size='340' side='right'caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O78 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria], [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O78 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene~~></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o77\|3o77]], [[3ek4\|3ek4]], [[3ek7\|3ek7]], [[3ek8\|3ek8]], [[3ekh\|3ekh]], [[3ekj\|3ekj]], [[3evp\|3evp]], [[3evr\|3evr]], [[3evu\|3evu]], [[3evv\|3evv]]</div></td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/[Myosin_light-chain]_kinase [Myosin light-chain] kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.18 2.7.11.18] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [https://pdbe.org/3o78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [https://www.ebi.ac.uk/pdbsum/3o78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o78 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [https://pdbe.org/3o78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [https://www.ebi.ac.uk/pdbsum/3o78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o78 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of CPVT4. The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of LQT14.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MYLK_CHICK MYLK_CHICK]] Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.	+	[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).<ref>PMID:16760425</ref> <ref>PMID:23893133</ref> <ref>PMID:26969752</ref> <ref>PMID:27165696</ref> [https://www.uniprot.org/uniprot/MYLK_CHICK MYLK_CHICK] Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aequorea victoria]]
		+	[[Category: Gallus gallus]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Britanova~~, O V~~]]	+	[[Category: Britanova OV]]
-	[[Category: Chudakov~~, D M~~]]	+	[[Category: Chudakov DM]]
-	[[Category: Freuler, F]]	+	[[Category: Freuler F]]
-	[[Category: Leder, L]]	+	[[Category: Leder L]]
-	[[Category: Marsh, M]]	+	[[Category: Marsh M]]
-	[[Category: Mayr~~, L M~~]]	+	[[Category: Mayr LM]]
-	[[Category: Meyerhofer, M]]	+	[[Category: Meyerhofer M]]
-	[[Category: Stark, W]]	+	[[Category: Stark W]]
-	[[Category: Stettler, T]]	+	[[Category: Stettler T]]
-	[[Category: Strukova~~, L A]]~~	+	[[Category: Strukova LA]]
-	~~[[Category: Calcium sensor]]~~	+
-	~~[[Category: Circular permutated green fluorescent protein]]~~	+
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Genetically encoded]]~~	+
-	~~[[Category: Gfp calmodulin m13-peptide]]~~	+
-	~~[[Category: Gyg naturally modified tripeptide acts as chromophore]]~~	+
-	~~[[Category: M13-peptide~~]]	+

OCA at 07:17, 12 May 2022

2022-05-12T07:17:42Z

OCA at 16:29, 28 August 2019

2019-08-28T16:29:30Z

←Older revision		Revision as of 16:29, 28 August 2019
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	==The structure of Ca2+ Sensor (Case-12)==		==The structure of Ca2+ Sensor (Case-12)==
-	<StructureSection load='3o78' size='340' side='right' caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='3o78' size='340' side='right'caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O78 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O78 FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 3o78" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3o78" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Calmodulin 3D structures\|Calmodulin 3D structures]]
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Britanova, O V]]		[[Category: Britanova, O V]]
	[[Category: Chudakov, D M]]		[[Category: Chudakov, D M]]

OCA at 09:17, 16 November 2017

2017-11-16T09:17:12Z

←Older revision		Revision as of 09:17, 16 November 2017
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 3o78" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3o78" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Calmodulin\|Calmodulin]]
-	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:42, 3 August 2017

2017-08-03T10:42:24Z

←Older revision		Revision as of 10:42, 3 August 2017
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	<StructureSection load='3o78' size='340' side='right' caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3o78' size='340' side='right' caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Chick Chick]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O78 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O78 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o77\|3o77]], [[3ek4\|3ek4]], [[3ek7\|3ek7]], [[3ek8\|3ek8]], [[3ekh\|3ekh]], [[3ekj\|3ekj]], [[3evp\|3evp]], [[3evr\|3evr]], [[3evu\|3evu]], [[3evv\|3evv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o77\|3o77]], [[3ek4\|3ek4]], [[3ek7\|3ek7]], [[3ek8\|3ek8]], [[3ekh\|3ekh]], [[3ekj\|3ekj]], [[3evp\|3evp]], [[3evr\|3evr]], [[3evu\|3evu]], [[3evv\|3evv]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_light-chain]_kinase [Myosin light-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.18 2.7.11.18] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [http://pdbe.org/3o78 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [http://www.ebi.ac.uk/pdbsum/3o78 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o78 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [http://pdbe.org/3o78 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [http://www.ebi.ac.uk/pdbsum/3o78 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o78 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chick]]
	[[Category: Britanova, O V]]		[[Category: Britanova, O V]]
	[[Category: Chudakov, D M]]		[[Category: Chudakov, D M]]

OCA at 01:40, 5 August 2016

2016-08-05T01:40:20Z

←Older revision		Revision as of 01:40, 5 August 2016
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		+
	==The structure of Ca2+ Sensor (Case-12)==		==The structure of Ca2+ Sensor (Case-12)==
	<StructureSection load='3o78' size='340' side='right' caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3o78' size='340' side='right' caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aequorea_victoria Aequorea victoria~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O78 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O78 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o77\|3o77]], [[3ek4\|3ek4]], [[3ek7\|3ek7]], [[3ek8\|3ek8]], [[3ekh\|3ekh]], [[3ekj\|3ekj]], [[3evp\|3evp]], [[3evr\|3evr]], [[3evu\|3evu]], [[3evv\|3evv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o77\|3o77]], [[3ek4\|3ek4]], [[3ek7\|3ek7]], [[3ek8\|3ek8]], [[3ekh\|3ekh]], [[3ekj\|3ekj]], [[3evp\|3evp]], [[3evr\|3evr]], [[3evu\|3evu]], [[3evv\|3evv]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [http://www.ebi.ac.uk/pdbsum/3o78 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [http://pdbe.org/3o78 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [http://www.ebi.ac.uk/pdbsum/3o78 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o78 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3o78" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aequorea victoria~~]]	+	[[Category: Chick]]
	[[Category: Britanova, O V]]		[[Category: Britanova, O V]]
	[[Category: Chudakov, D M]]		[[Category: Chudakov, D M]]

OCA at 14:46, 25 December 2014

2014-12-25T14:46:46Z

←Older revision		Revision as of 14:46, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [http://www.ebi.ac.uk/pdbsum/3o78 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [http://www.ebi.ac.uk/pdbsum/3o78 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MYLK_CHICK MYLK_CHICK]] Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:09, 21 December 2014

2014-12-21T18:09:45Z

←Older revision		Revision as of 18:09, 21 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3o78.~~png~~\|~~left~~\|~~200px~~]]	+	==The structure of Ca2+ Sensor (Case-12)==
		+	<StructureSection load='3o78' size='340' side='right' caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O78 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o77\|3o77]], [[3ek4\|3ek4]], [[3ek7\|3ek7]], [[3ek8\|3ek8]], [[3ekh\|3ekh]], [[3ekj\|3ekj]], [[3evp\|3evp]], [[3evr\|3evr]], [[3evu\|3evu]], [[3evv\|3evv]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [http://www.ebi.ac.uk/pdbsum/3o78 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Here we report the first crystal structure of a high-contrast genetically encoded circularly permuted green fluorescent protein (cpGFP)-based Ca(2+) sensor, Case16, in the presence of a low Ca(2+) concentration. The structure reveals the positioning of the chromophore within Case16 at the first stage of the Ca(2+)-dependent response when only two out of four Ca(2+)-binding pockets of calmodulin (CaM) are occupied with Ca(2+) ions. In such a "half Ca(2+)-bound state", Case16 is characterized by an incomplete interaction between its CaM-/M13-domains. We also report the crystal structure of the related Ca(2+) sensor Case12 at saturating Ca(2+) concentration. Based on this structure, we postulate that cpGFP-based Ca(2+) sensors can form non-functional homodimers where the CaM-domain of one sensor molecule binds symmetrically to the M13-peptide of the partner sensor molecule. Case12 and Case16 behavior upon addition of high concentrations of free CaM or M13-peptide reveals that the latter effectively blocks the fluorescent response of the sensor. We speculate that the demonstrated intermolecular interaction with endogenous substrates and homodimerization can impede proper functioning of this type of Ca(2+) sensors in living cells.

-	~~{{STRUCTURE_3o78\| PDB=3o78 \| SCENE= }}~~	+	The structure of Ca2+ sensor Case16 reveals the mechanism of reaction to low Ca2+ concentrations.,Leder L, Stark W, Freuler F, Marsh M, Meyerhofer M, Stettler T, Mayr LM, Britanova OV, Strukova LA, Chudakov DM, Souslova EA Sensors (Basel). 2010;10(9):8143-60. Epub 2010 Aug 30. PMID:22163646<ref>PMID:22163646</ref>

-	~~===The structure~~ of ~~Ca2+ Sensor (Case-12)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22163646}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3o78]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aequorea_victoria Aequorea victoria]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA].~~	+

	==See Also==		==See Also==
-	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
	*[[Calmodulin\|Calmodulin]]		*[[Calmodulin\|Calmodulin]]
-	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]		*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
-	*[[Sandbox104\|Sandbox104]]	+	== References ==
-	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:022163646<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Aequorea victoria]]		[[Category: Aequorea victoria]]
-	[[Category: Britanova, O V.]]	+	[[Category: Britanova, O V]]
-	[[Category: Chudakov, D M.]]	+	[[Category: Chudakov, D M]]
-	[[Category: Freuler, F.]]	+	[[Category: Freuler, F]]
-	[[Category: Leder, L.]]	+	[[Category: Leder, L]]
-	[[Category: Marsh, M.]]	+	[[Category: Marsh, M]]
-	[[Category: Mayr, L M.]]	+	[[Category: Mayr, L M]]
-	[[Category: Meyerhofer, M.]]	+	[[Category: Meyerhofer, M]]
-	[[Category: Stark, W.]]	+	[[Category: Stark, W]]
-	[[Category: Stettler, T.]]	+	[[Category: Stettler, T]]
-	[[Category: Strukova, L A.]]	+	[[Category: Strukova, L A]]
	[[Category: Calcium sensor]]		[[Category: Calcium sensor]]
	[[Category: Circular permutated green fluorescent protein]]		[[Category: Circular permutated green fluorescent protein]]

OCA at 17:59, 20 October 2012

2012-10-20T17:59:05Z

←Older revision		Revision as of 17:59, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3o78]] is a 2 chain structure ~~of [[Alyssa Marsico/Sandbox 1]], [[Calmodulin]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]]~~ with sequence from [http://en.wikipedia.org/wiki/~~Gallus_gallus,_aequorea_victoria,_aequorea_victoria,_homo_sapiens Gallus gallus, aequorea victoria, aequorea~~ victoria~~, homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA].	+	[[3o78]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA].

	==See Also==		==See Also==
Line 20:		Line 20:
	==Reference==		==Reference==
	<ref group="xtra">PMID:022163646</ref><references group="xtra"/>		<ref group="xtra">PMID:022163646</ref><references group="xtra"/>
-	[[Category: ~~Gallus gallus, aequorea~~ victoria~~, aequorea victoria, homo sapiens~~]]	+	[[Category: Aequorea victoria]]
	[[Category: Britanova, O V.]]		[[Category: Britanova, O V.]]
	[[Category: Chudakov, D M.]]		[[Category: Chudakov, D M.]]

OCA at 02:37, 27 July 2012

2012-07-27T02:37:28Z

←Older revision		Revision as of 02:37, 27 July 2012
Line 1:		Line 1:
	[[Image:3o78.png\|left\|200px]]		[[Image:3o78.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3o78\| PDB=3o78 \| SCENE= }}		{{STRUCTURE_3o78\| PDB=3o78 \| SCENE= }}

	===The structure of Ca2+ Sensor (Case-12)===		===The structure of Ca2+ Sensor (Case-12)===

-
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←Older revision		Revision as of 07:17, 12 May 2022
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	<StructureSection load='3o78' size='340' side='right'caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3o78' size='340' side='right'caption='[[3o78]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3O78 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o78]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O78 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o77\|3o77]], [[3ek4\|3ek4]], [[3ek7\|3ek7]], [[3ek8\|3ek8]], [[3ekh\|3ekh]], [[3ekj\|3ekj]], [[3evp\|3evp]], [[3evr\|3evr]], [[3evu\|3evu]], [[3evv\|3evv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o77\|3o77]], [[3ek4\|3ek4]], [[3ek7\|3ek7]], [[3ek8\|3ek8]], [[3ekh\|3ekh]], [[3ekj\|3ekj]], [[3evp\|3evp]], [[3evr\|3evr]], [[3evu\|3evu]], [[3evv\|3evv]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/[Myosin_light-chain]_kinase [Myosin light-chain] kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.18 2.7.11.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/[Myosin_light-chain]_kinase [Myosin light-chain] kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.18 2.7.11.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [~~http~~://pdbe.org/3o78 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3o78 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3o78 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o78 OCA], [https://pdbe.org/3o78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o78 RCSB], [https://www.ebi.ac.uk/pdbsum/3o78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o78 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MYLK_CHICK MYLK_CHICK]] Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.	+	[[https://www.uniprot.org/uniprot/MYLK_CHICK MYLK_CHICK]] Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==