3owy - Revision history

OCA at 09:45, 6 September 2023

2023-09-06T09:45:19Z

←Older revision		Revision as of 09:45, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3owy' size='340' side='right'caption='[[3owy]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3owy' size='340' side='right'caption='[[3owy]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3owy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OWY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3owy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OWY FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EQU:EQUILENIN'>EQU</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non~~-~~Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XCN:S-CYANO-L-CYSTEINE'>XCN</scene></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EQU:EQUILENIN'>EQU</scene>, <scene name='pdbligand=XCN:S-CYANO-L-CYSTEINE'>XCN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3ows\|3ows]], [[3owu\|3owu]]</div>~~</td></tr>	+
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ksi ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~303~~ "~~Bacillus fluorescens putidus~~" ~~Flugge 1886])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Steroid_Delta~~-~~isomerase Steroid Delta~~-~~isomerase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owy OCA], [https://pdbe.org/3owy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3owy RCSB], [https://www.ebi.ac.uk/pdbsum/3owy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3owy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owy OCA], [https://pdbe.org/3owy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3owy RCSB], [https://www.ebi.ac.uk/pdbsum/3owy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3owy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SDIS_PSEPU SDIS_PSEPU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Steroid Delta-isomerase~~]]	+	[[Category: Pseudomonas putida]]
-	[[Category: Fenn~~, T D~~]]	+	[[Category: Fenn TD]]
-	[[Category: Herschlag, D]]	+	[[Category: Herschlag D]]
-	[[Category: Sigala~~, P A]]~~	+	[[Category: Sigala PA]]
-	~~[[Category: Cyanylation]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Steroid~~]]	+

OCA at 11:00, 18 May 2022

2022-05-18T11:00:58Z

OCA at 21:10, 5 August 2016

2016-08-05T21:10:17Z

←Older revision		Revision as of 21:10, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin==		==Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin==
	<StructureSection load='3owy' size='340' side='right' caption='[[3owy]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3owy' size='340' side='right' caption='[[3owy]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3owy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_putida Pseudomonas putida~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OWY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3owy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OWY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQU:EQUILENIN'>EQU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQU:EQUILENIN'>EQU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XCN:S-CYANO-L-CYSTEINE'>XCN</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XCN:S-CYANO-L-CYSTEINE'>XCN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ows\|3ows]], [[3owu\|3owu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ows\|3ows]], [[3owu\|3owu]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 ~~Pseudomonas putida~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3owy RCSB], [http://www.ebi.ac.uk/pdbsum/3owy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owy OCA], [http://pdbe.org/3owy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3owy RCSB], [http://www.ebi.ac.uk/pdbsum/3owy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3owy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3owy" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas putida~~]]	+	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
	[[Category: Fenn, T D]]		[[Category: Fenn, T D]]

OCA at 21:18, 3 January 2015

2015-01-03T21:18:26Z

←Older revision		Revision as of 21:18, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3owy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OWY FirstGlance]. <br>		<table><tr><td colspan='2'>[[3owy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OWY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQU:EQUILENIN'>EQU</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQU:EQUILENIN'>EQU</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XCN:S-CYANO-L-CYSTEINE'>XCN</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XCN:S-CYANO-L-CYSTEINE'>XCN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ows\|3ows]], [[3owu\|3owu]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ows\|3ows]], [[3owu\|3owu]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3owy RCSB], [http://www.ebi.ac.uk/pdbsum/3owy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3owy RCSB], [http://www.ebi.ac.uk/pdbsum/3owy PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 16:
	Quantitative, directional measurement of electric field heterogeneity in the active site of ketosteroid isomerase.,Fafarman AT, Sigala PA, Schwans JP, Fenn TD, Herschlag D, Boxer SG Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):E299-308. Epub 2012 Jan 17. PMID:22308339<ref>PMID:22308339</ref>		Quantitative, directional measurement of electric field heterogeneity in the active site of ketosteroid isomerase.,Fafarman AT, Sigala PA, Schwans JP, Fenn TD, Herschlag D, Boxer SG Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):E299-308. Epub 2012 Jan 17. PMID:22308339<ref>PMID:22308339</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
-	[[Category: Fenn, T D.]]	+	[[Category: Fenn, T D]]
-	[[Category: Herschlag, D.]]	+	[[Category: Herschlag, D]]
-	[[Category: Sigala, P A.]]	+	[[Category: Sigala, P A]]
	[[Category: Cyanylation]]		[[Category: Cyanylation]]
	[[Category: Isomerase]]		[[Category: Isomerase]]
	[[Category: Steroid]]		[[Category: Steroid]]

OCA at 10:28, 28 May 2014

2014-05-28T10:28:51Z

←Older revision		Revision as of 10:28, 28 May 2014
Line 1:		Line 1:
-	[[~~Image:~~3owy.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin==
		+	<StructureSection load='3owy' size='340' side='right' caption='[[3owy]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3owy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OWY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQU:EQUILENIN'>EQU</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XCN:S-CYANO-L-CYSTEINE'>XCN</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ows\|3ows]], [[3owu\|3owu]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3owy RCSB], [http://www.ebi.ac.uk/pdbsum/3owy PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Understanding the electrostatic forces and features within highly heterogeneous, anisotropic, and chemically complex enzyme active sites and their connection to biological catalysis remains a longstanding challenge, in part due to the paucity of incisive experimental probes of electrostatic properties within proteins. To quantitatively assess the landscape of electrostatic fields at discrete locations and orientations within an enzyme active site, we have incorporated site-specific thiocyanate vibrational probes into multiple positions within bacterial ketosteroid isomerase. A battery of X-ray crystallographic, vibrational Stark spectroscopy, and NMR studies revealed electrostatic field heterogeneity of 8 MV/cm between active site probe locations and widely differing sensitivities of discrete probes to common electrostatic perturbations from mutation, ligand binding, and pH changes. Electrostatic calculations based on active site ionization states assigned by literature precedent and computational pK(a) prediction were unable to quantitatively account for the observed vibrational band shifts. However, electrostatic models of the D40N mutant gave qualitative agreement with the observed vibrational effects when an unusual ionization of an active site tyrosine with a pK(a) near 7 was included. UV-absorbance and (13)C NMR experiments confirmed the presence of a tyrosinate in the active site, in agreement with electrostatic models. This work provides the most direct measure of the heterogeneous and anisotropic nature of the electrostatic environment within an enzyme active site, and these measurements provide incisive benchmarks for further developing accurate computational models and a foundation for future tests of electrostatics in enzymatic catalysis.

-	~~<!--~~	+	Quantitative, directional measurement of electric field heterogeneity in the active site of ketosteroid isomerase.,Fafarman AT, Sigala PA, Schwans JP, Fenn TD, Herschlag D, Boxer SG Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):E299-308. Epub 2012 Jan 17. PMID:22308339<ref>PMID:22308339</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3owy", creates the "Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3owy\| PDB=3owy \| SCENE= }}~~	+

-	~~===Crystal Structure of Ketosteroid Isomerase D40N~~/~~C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22308339}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22308339 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22308339}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3owy]] is a 8 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022308339<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]

OCA at 05:56, 15 February 2012

2012-02-15T05:56:32Z

←Older revision		Revision as of 05:56, 15 February 2012
Line 1:		Line 1:
-	[[Image:3owy.~~jpg~~\|left\|200px]]	+	[[Image:3owy.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin===		===Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22308339}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22308339 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22308339}}

	==About this Structure==		==About this Structure==
	[[3owy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA].		[[3owy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022308339</ref><references group="xtra"/>
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]

OCA at 06:07, 21 September 2011

2011-09-21T06:07:20Z

←Older revision		Revision as of 06:07, 21 September 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3owy.jpg\|left\|200px]]

-	The ~~entry 3owy~~ is ~~ON HOLD~~ ~~until Sep 21 2012~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3owy", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3owy\| PDB=3owy \| SCENE= }}

-	~~Authors: Sigala,~~ P.~~A., Fenn, T.D., Herschlag, D.~~	+	===Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin===

-	~~Description: Crystal~~ Structure ~~of Ketosteroid Isomerase D40N~~/~~C69S~~/~~C81S~~/~~C97S~~/~~M105C-CN~~ from P. putida ~~with Bound Equilenin~~	+
		+	==About this Structure==
		+	[[3owy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA].
		+	[[Category: Pseudomonas putida]]
		+	[[Category: Steroid Delta-isomerase]]
		+	[[Category: Fenn, T D.]]
		+	[[Category: Herschlag, D.]]
		+	[[Category: Sigala, P A.]]
		+	[[Category: Cyanylation]]
		+	[[Category: Isomerase]]
		+	[[Category: Steroid]]

OCA: Protected "3owy" [edit=sysop:move=sysop]

2011-08-03T08:13:17Z

Protected "3owy" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:13, 3 August 2011

OCA at 08:13, 3 August 2011

2011-08-03T08:13:16Z

←Older revision		Revision as of 08:13, 3 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3owy is ON HOLD until ~~Paper Publication~~	+	The entry 3owy is ON HOLD until Sep 21 2012

	Authors: Sigala, P.A., Fenn, T.D., Herschlag, D.		Authors: Sigala, P.A., Fenn, T.D., Herschlag, D.

	Description: Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin		Description: Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 29 07:57:59 2010''

OCA: New page: '''Unreleased structure''' The entry 3owy is ON HOLD until Paper Publication Authors: Sigala, P.A., Fenn, T.D., Herschlag, D. Description: Crystal Structure of Ketosteroid Isomerase D4...

2010-09-29T04:48:44Z

New page: '''Unreleased structure''' The entry 3owy is ON HOLD until Paper Publication Authors: Sigala, P.A., Fenn, T.D., Herschlag, D. Description: Crystal Structure of Ketosteroid Isomerase D4...

New page

'''Unreleased structure'''

The entry 3owy is ON HOLD until Paper Publication

Authors: Sigala, P.A., Fenn, T.D., Herschlag, D.

Description: Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 29 07:57:59 2010''

←Older revision		Revision as of 11:00, 18 May 2022
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	==Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin==		==Crystal Structure of Ketosteroid Isomerase D40N/C69S/C81S/C97S/M105C-CN from P. putida with Bound Equilenin==
-	<StructureSection load='3owy' size='340' side='right' caption='[[3owy]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3owy' size='340' side='right'caption='[[3owy]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3owy]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3OWY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3owy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OWY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQU:EQUILENIN'>EQU</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EQU:EQUILENIN'>EQU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XCN:S-CYANO-L-CYSTEINE'>XCN</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=XCN:S-CYANO-L-CYSTEINE'>XCN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ows\|3ows]], [[3owu\|3owu]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ows\|3ows]], [[3owu\|3owu]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owy OCA], [~~http~~://pdbe.org/3owy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3owy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3owy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3owy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3owy OCA], [https://pdbe.org/3owy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3owy RCSB], [https://www.ebi.ac.uk/pdbsum/3owy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3owy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus fluorescens putidus flugge 1886]]		[[Category: Bacillus fluorescens putidus flugge 1886]]
		+	[[Category: Large Structures]]
	[[Category: Steroid Delta-isomerase]]		[[Category: Steroid Delta-isomerase]]
	[[Category: Fenn, T D]]		[[Category: Fenn, T D]]