3pf3 - Revision history

OCA at 09:53, 6 September 2023

2023-09-06T09:53:02Z

←Older revision		Revision as of 09:53, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3pf3' size='340' side='right'caption='[[3pf3]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3pf3' size='340' side='right'caption='[[3pf3]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pf3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Giain Giain~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PF3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pf3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PF3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.098Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~SCH~~:~~S-METHYL-THIO-CYSTEINE~~'>~~SCH~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SCH:S-METHYL-THIO-CYSTEINE'>SCH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class="sblockDat"><div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2dp3\|2dp3]]~~</~~div~~><~~/td></tr>~~	+
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">GLTIM ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5741 GIAIN])</td></tr>~~	+
-	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf3 OCA], [https://pdbe.org/3pf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pf3 RCSB], [https://www.ebi.ac.uk/pdbsum/3pf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf3 OCA], [https://pdbe.org/3pf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pf3 RCSB], [https://www.ebi.ac.uk/pdbsum/3pf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TPI1_GIAIN TPI1_GIAIN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Giain~~]]	+	[[Category: Giardia intestinalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Triose-phosphate isomerase]]~~	+	[[Category: Enriquez-Flores S]]
-	[[Category: Enriquez-Flores, S]]	+	[[Category: Hernandez-Santoyo A]]
-	[[Category: Hernandez-Santoyo, A]]	+	[[Category: Reyes-Vivas H]]
-	[[Category: Reyes-Vivas, H]]	+	[[Category: Rodriguez-Romero A]]
-	[[Category: Rodriguez-Romero, A]]	+
-	~~[[Category: Alfa/beta barrel]]~~	+
-	~~[[Category: Alpha/beta barrel]]~~	+
-	~~[[Category: Cys 228]]~~	+
-	~~[[Category: Cys222]]~~	+
-	~~[[Category: Giardia]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Thiomethylation]]~~	+
-	~~[[Category: Thiomethylation of cys14]]~~	+
-	~~[[Category: Triosephosphate isomerase~~]]	+

OCA at 08:36, 25 May 2022

2022-05-25T08:36:43Z

←Older revision		Revision as of 08:36, 25 May 2022
Line 1:		Line 1:

	==Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS==		==Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS==
-	<StructureSection load='3pf3' size='340' side='right' caption='[[3pf3]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3pf3' size='340' side='right'caption='[[3pf3]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pf3]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Giain Giain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PF3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pf3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Giain Giain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PF3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCH:S-METHYL-THIO-CYSTEINE'>SCH</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCH:S-METHYL-THIO-CYSTEINE'>SCH</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dp3\|2dp3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2dp3\|2dp3]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLTIM ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5741 GIAIN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLTIM ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5741 GIAIN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf3 OCA], [~~http~~://pdbe.org/3pf3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3pf3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3pf3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf3 OCA], [https://pdbe.org/3pf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pf3 RCSB], [https://www.ebi.ac.uk/pdbsum/3pf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Triose ~~Phosphate Isomerase~~\|Triose ~~Phosphate Isomerase~~]]	+	*[[Triose phosphate isomerase 3D structures\|Triose phosphate isomerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Giain]]		[[Category: Giain]]
		+	[[Category: Large Structures]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
	[[Category: Enriquez-Flores, S]]		[[Category: Enriquez-Flores, S]]

OCA at 20:13, 5 August 2016

2016-08-05T20:13:12Z

←Older revision		Revision as of 20:13, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS==		==Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS==
	<StructureSection load='3pf3' size='340' side='right' caption='[[3pf3]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3pf3' size='340' side='right' caption='[[3pf3]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pf3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Giardia_intestinalis Giardia intestinalis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pf3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giain Giain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCH:S-METHYL-THIO-CYSTEINE'>SCH</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCH:S-METHYL-THIO-CYSTEINE'>SCH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dp3\|2dp3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dp3\|2dp3]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLTIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5741 ~~Giardia intestinalis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLTIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5741 GIAIN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pf3 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf3 OCA], [http://pdbe.org/3pf3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pf3 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3pf3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Giardia intestinalis~~]]	+	[[Category: Giain]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
	[[Category: Enriquez-Flores, S]]		[[Category: Enriquez-Flores, S]]

OCA at 08:32, 18 December 2014

2014-12-18T08:32:18Z

←Older revision		Revision as of 08:32, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3pf3\| PDB=3pf3 \| SCENE= }}~~	+	==Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS==
-	===Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS===	+	<StructureSection load='3pf3' size='340' side='right' caption='[[3pf3]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21786322}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3pf3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF3 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCH:S-METHYL-THIO-CYSTEINE'>SCH</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dp3\|2dp3]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLTIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5741 Giardia intestinalis])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pf3 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf3 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Giardiasis, the most prevalent intestinal parasitosis in humans, is caused by Giardia lamblia. Current drug therapies have adverse effects on the host, and resistant strains against these drugs have been reported, demonstrating an urgent need to design more specific antigiardiasic drugs. ATP production in G. lamblia depends mainly on glycolysis; therefore, all enzymes of this pathway have been proposed as potential drug targets. We previously demonstrated that the glycolytic enzyme triosephosphate isomerase from G. lamblia (GlTIM), could be completely inactivated by low micromolar concentrations of thiol-reactive compounds, whereas, in the same conditions, the activity of human TIM (HuTIM) was almost unaltered. We found that the chemical modification (derivatization) of at least one Cys, of the five Cys residues per monomer in GlTIM, causes this inactivation. In this study, structural and functional studies were performed to describe the molecular mechanism of GlTIM inactivation by thiol-reactive compounds. We found that the Cys222 derivatization is responsible for GlTIM inactivation; this information is relevant because HuTIM has a Cys residue in an equivalent position (Cys217). GlTIM inactivation is associated with a decrease in ligand affinity, which affects the entropic component of ligand binding. In summary, this work describes a mechanism of inactivation that has not been previously reported for TIMs from other parasites and furthermore, we show that the difference in reactivity between the Cys222 in GlTIM and the Cys217 in HuTIM, indicates that the surrounding environment of each Cys residue has unique structural differences that can be exploited to design specific antigiardiasic drugs. Proteins 2011;. (c) 2011 Wiley-Liss, Inc.

-	~~==About this Structure==~~	+	Determining the molecular mechanism of inactivation by chemical modification of triosephosphate isomerase from the human parasite Giardia lamblia: A study for antiparasitic drug design.,Enriquez-Flores S, Rodriguez-Romero A, Hernandez-Alcantara G, Oria-Hernandez J, Gutierrez-Castrellon P, Perez-Hernandez G, Mora Ide L, Castillo-Villanueva A, Garcia-Torres I, Mendez ST, Gomez-Manzo S, Torres-Arroyo A, Lopez-Velazquez G, Reyes-Vivas H Proteins. 2011 Sep;79(9):2711-24. doi: 10.1002/prot.23100. Epub 2011 Jul, 22. PMID:21786322<ref>PMID:21786322</ref>
-	~~[[3pf3]] is a 1 chain structure with sequence~~ from ~~[http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Giardia_intestinalis Giardia intestinalis]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin~~/~~ocashort?id=3PF3 OCA]~~.	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]		*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021786322<~~/~~ref~~><~~ref group="xtra">PMID:017095008</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Giardia intestinalis]]		[[Category: Giardia intestinalis]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
-	[[Category: Enriquez-Flores, S.]]	+	[[Category: Enriquez-Flores, S]]
-	[[Category: Hernandez-Santoyo, A.]]	+	[[Category: Hernandez-Santoyo, A]]
-	[[Category: Reyes-Vivas, H.]]	+	[[Category: Reyes-Vivas, H]]
-	[[Category: Rodriguez-Romero, A.]]	+	[[Category: Rodriguez-Romero, A]]
	[[Category: Alfa/beta barrel]]		[[Category: Alfa/beta barrel]]
	[[Category: Alpha/beta barrel]]		[[Category: Alpha/beta barrel]]

OCA at 09:54, 11 March 2013

2013-03-11T09:54:05Z

←Older revision		Revision as of 09:54, 11 March 2013
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-	[[Image:3pf3.png\|left\|200px]]
-
	{{STRUCTURE_3pf3\| PDB=3pf3 \| SCENE= }}		{{STRUCTURE_3pf3\| PDB=3pf3 \| SCENE= }}
-
	===Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS===		===Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS===
-
	{{ABSTRACT_PUBMED_21786322}}		{{ABSTRACT_PUBMED_21786322}}

OCA at 13:10, 20 October 2012

2012-10-20T13:10:51Z

←Older revision		Revision as of 13:10, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3pf3]] is a 1 chain structure ~~of [[Triose Phosphate Isomerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA].	+	[[3pf3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA].

	==See Also==		==See Also==

OCA at 02:48, 29 August 2012

2012-08-29T02:48:31Z

←Older revision		Revision as of 02:48, 29 August 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3pf3]] is a 1 chain structure of ~~[[TIM test]] and~~ [[Triose Phosphate Isomerase]] with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA].	+	[[3pf3]] is a 1 chain structure of [[Triose Phosphate Isomerase]] with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA].

	==See Also==		==See Also==
-	*[[TIM test\|TIM test]]
	*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]		*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]

OCA at 11:08, 26 July 2012

2012-07-26T11:08:47Z

←Older revision		Revision as of 11:08, 26 July 2012
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	[[Image:3pf3.png\|left\|200px]]		[[Image:3pf3.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3pf3", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3pf3\| PDB=3pf3 \| SCENE= }}		{{STRUCTURE_3pf3\| PDB=3pf3 \| SCENE= }}

	===Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS===		===Crystal structure of a mutant (C202A) of Triosephosphate isomerase from Giardia lamblia derivatized with MMTS===

-
-	<!--
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	{{ABSTRACT_PUBMED_21786322}}		{{ABSTRACT_PUBMED_21786322}}

	==About this Structure==		==About this Structure==
-	[[3pf3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA].	+	[[3pf3]] is a 1 chain structure of [[TIM test]] and [[Triose Phosphate Isomerase]] with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF3 OCA].
		+
		+	==See Also==
		+	*[[TIM test\|TIM test]]
		+	*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]

	==Reference==		==Reference==

OCA at 05:48, 24 August 2011

2011-08-24T05:48:14Z

←Older revision		Revision as of 05:48, 24 August 2011
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-	[[Image:3pf3.~~jpg~~\|left\|200px]]	+	[[Image:3pf3.png\|left\|200px]]

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	<!--		<!--
-	The line below this paragraph, {{~~ABSTRACT_PUBMED_17095008~~}}, adds the Publication Abstract to the page	+	The line below this paragraph, {{ABSTRACT_PUBMED_21786322}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where ~~17095008~~ is the PubMed ID number.	+	(as it appears on PubMed at http://www.pubmed.gov), where 21786322 is the PubMed ID number.
	-->		-->
-	{{~~ABSTRACT_PUBMED_17095008~~}}	+	{{ABSTRACT_PUBMED_21786322}}

	==About this Structure==		==About this Structure==
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:017095008</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021786322</ref><ref group="xtra">PMID:017095008</ref><references group="xtra"/>
	[[Category: Giardia intestinalis]]		[[Category: Giardia intestinalis]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]

OCA: Protected "3pf3" [edit=sysop:move=sysop]

2011-06-22T16:48:39Z

Protected "3pf3" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:48, 22 June 2011