3qcp - Revision history

OCA at 11:41, 14 March 2024

2024-03-14T11:41:06Z

←Older revision		Revision as of 11:41, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3qcp' size='340' side='right'caption='[[3qcp]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3qcp' size='340' side='right'caption='[[3qcp]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qcp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Trypanosoma_(trypanozoon)_brucei~~ Trypanosoma ~~(trypanozoon)~~ brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QCP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qcp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QCP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3qd9\|3qd9]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>QSOX, Tb927.6.1850 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~5691 Trypanosoma (Trypanozoon) brucei])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Thiol_oxidase Thiol oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.3.2 1.8.3.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qcp OCA], [https://pdbe.org/3qcp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qcp RCSB], [https://www.ebi.ac.uk/pdbsum/3qcp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qcp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qcp OCA], [https://pdbe.org/3qcp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qcp RCSB], [https://www.ebi.ac.uk/pdbsum/3qcp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qcp ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q585M6_TRYB2 Q585M6_TRYB2]
-	Protein stability, assembly, localization and regulation often depend on the formation of disulphide crosslinks between cysteine side chains. Enzymes known as sulphydryl oxidases catalyse de novo disulphide formation and initiate intra- and intermolecular dithiol/disulphide relays to deliver the disulphides to substrate proteins. Quiescin sulphydryl oxidase (QSOX) is a unique, multi-domain disulphide catalyst that is localized primarily to the Golgi apparatus and secreted fluids and has attracted attention owing to its overproduction in tumours. In addition to its physiological importance, QSOX is a mechanistically intriguing enzyme, encompassing functions typically carried out by a series of proteins in other disulphide-formation pathways. How disulphides are relayed through the multiple redox-active sites of QSOX and whether there is a functional benefit to concatenating these sites on a single polypeptide are open questions. Here we present the first crystal structure of an intact QSOX enzyme, derived from a trypanosome parasite. Notably, sequential sites in the disulphide relay were found more than 40 A apart in this structure, too far for direct disulphide transfer. To resolve this puzzle, we trapped and crystallized an intermediate in the disulphide hand-off, which showed a 165 degrees domain rotation relative to the original structure, bringing the two active sites within disulphide-bonding distance. The comparable structure of a mammalian QSOX enzyme, also presented here, shows further biochemical features that facilitate disulphide transfer in metazoan orthologues. Finally, we quantified the contribution of concatenation to QSOX activity, providing general lessons for the understanding of multi-domain enzymes and the design of new catalytic relays.	+
-		+
-	~~The dynamic disulphide relay of quiescin sulphydryl oxidase.,Alon A, Grossman I, Gat Y, Kodali VK, DiMaio F, Mehlman T, Haran G, Baker D, Thorpe C, Fass D Nature. 2012 Aug 16;488(7411)~~:~~414-8. PMID:22801504<ref>PMID:22801504<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3qcp" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Sulfhydryl oxidase 3D structures\|Sulfhydryl oxidase 3D structures]]		*[[Sulfhydryl oxidase 3D structures\|Sulfhydryl oxidase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thiol oxidase~~]]	+	[[Category: Trypanosoma brucei]]
-	[[Category: Alon, A]]	+	[[Category: Alon A]]
-	[[Category: Fass, D]]	+	[[Category: Fass D]]
-	~~[[Category: Erv fold]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Sulfhydryl oxidase]]~~	+
-	~~[[Category: Thioredoxin fold~~]]	+

OCA at 06:02, 8 June 2022

2022-06-08T06:02:27Z

←Older revision		Revision as of 06:02, 8 June 2022
Line 1:		Line 1:

	==QSOX from Trypanosoma brucei==		==QSOX from Trypanosoma brucei==
-	<StructureSection load='3qcp' size='340' side='right' caption='[[3qcp]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3qcp' size='340' side='right'caption='[[3qcp]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qcp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Trypanosoma_(trypanozoon)_brucei Trypanosoma (trypanozoon) brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QCP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qcp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_(trypanozoon)_brucei Trypanosoma (trypanozoon) brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QCP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qd9\|3qd9]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qd9\|3qd9]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QSOX, Tb927.6.1850 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5691 Trypanosoma (Trypanozoon) brucei])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QSOX, Tb927.6.1850 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5691 Trypanosoma (Trypanozoon) brucei])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Thiol_oxidase Thiol oxidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.3.2 1.8.3.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thiol_oxidase Thiol oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.3.2 1.8.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qcp OCA], [~~http~~://pdbe.org/3qcp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qcp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qcp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qcp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qcp OCA], [https://pdbe.org/3qcp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qcp RCSB], [https://www.ebi.ac.uk/pdbsum/3qcp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qcp ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3qcp" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3qcp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Sulfhydryl oxidase 3D structures\|Sulfhydryl oxidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Thiol oxidase]]		[[Category: Thiol oxidase]]
	[[Category: Alon, A]]		[[Category: Alon, A]]

OCA at 19:25, 5 August 2016

2016-08-05T19:25:38Z

←Older revision		Revision as of 19:25, 5 August 2016
Line 1:		Line 1:
		+
	==QSOX from Trypanosoma brucei==		==QSOX from Trypanosoma brucei==
	<StructureSection load='3qcp' size='340' side='right' caption='[[3qcp]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3qcp' size='340' side='right' caption='[[3qcp]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qcp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Trypanosoma_brucei~~ Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QCP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qcp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_(trypanozoon)_brucei Trypanosoma (trypanozoon) brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QCP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qd9\|3qd9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qd9\|3qd9]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QSOX, Tb927.6.1850 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5691 Trypanosoma brucei])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QSOX, Tb927.6.1850 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5691 Trypanosoma (Trypanozoon) brucei])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiol_oxidase Thiol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.3.2 1.8.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiol_oxidase Thiol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.3.2 1.8.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qcp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qcp RCSB], [http://www.ebi.ac.uk/pdbsum/3qcp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qcp OCA], [http://pdbe.org/3qcp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qcp RCSB], [http://www.ebi.ac.uk/pdbsum/3qcp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qcp ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qcp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Thiol oxidase]]		[[Category: Thiol oxidase]]
-	[[Category: Trypanosoma brucei]]
	[[Category: Alon, A]]		[[Category: Alon, A]]
	[[Category: Fass, D]]		[[Category: Fass, D]]

OCA at 22:18, 3 January 2015

2015-01-03T22:18:34Z

←Older revision		Revision as of 22:18, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3qcp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QCP FirstGlance]. <br>		<table><tr><td colspan='2'>[[3qcp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QCP FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qd9\|3qd9]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qd9\|3qd9]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QSOX, Tb927.6.1850 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5691 Trypanosoma brucei])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QSOX, Tb927.6.1850 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5691 Trypanosoma brucei])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiol_oxidase Thiol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.3.2 1.8.3.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiol_oxidase Thiol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.3.2 1.8.3.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qcp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qcp RCSB], [http://www.ebi.ac.uk/pdbsum/3qcp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qcp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qcp RCSB], [http://www.ebi.ac.uk/pdbsum/3qcp PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Thiol oxidase]]		[[Category: Thiol oxidase]]
	[[Category: Trypanosoma brucei]]		[[Category: Trypanosoma brucei]]
-	[[Category: Alon, A.]]	+	[[Category: Alon, A]]
-	[[Category: Fass, D.]]	+	[[Category: Fass, D]]
	[[Category: Erv fold]]		[[Category: Erv fold]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Sulfhydryl oxidase]]		[[Category: Sulfhydryl oxidase]]
	[[Category: Thioredoxin fold]]		[[Category: Thioredoxin fold]]

OCA at 07:11, 9 June 2014

2014-06-09T07:11:25Z

←Older revision		Revision as of 07:11, 9 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3qcp.~~png~~\|~~left~~\|~~200px~~]]	+	==QSOX from Trypanosoma brucei==
		+	<StructureSection load='3qcp' size='340' side='right' caption='[[3qcp]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3qcp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QCP FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qd9\|3qd9]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QSOX, Tb927.6.1850 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5691 Trypanosoma brucei])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiol_oxidase Thiol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.3.2 1.8.3.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qcp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qcp RCSB], [http://www.ebi.ac.uk/pdbsum/3qcp PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein stability, assembly, localization and regulation often depend on the formation of disulphide crosslinks between cysteine side chains. Enzymes known as sulphydryl oxidases catalyse de novo disulphide formation and initiate intra- and intermolecular dithiol/disulphide relays to deliver the disulphides to substrate proteins. Quiescin sulphydryl oxidase (QSOX) is a unique, multi-domain disulphide catalyst that is localized primarily to the Golgi apparatus and secreted fluids and has attracted attention owing to its overproduction in tumours. In addition to its physiological importance, QSOX is a mechanistically intriguing enzyme, encompassing functions typically carried out by a series of proteins in other disulphide-formation pathways. How disulphides are relayed through the multiple redox-active sites of QSOX and whether there is a functional benefit to concatenating these sites on a single polypeptide are open questions. Here we present the first crystal structure of an intact QSOX enzyme, derived from a trypanosome parasite. Notably, sequential sites in the disulphide relay were found more than 40 A apart in this structure, too far for direct disulphide transfer. To resolve this puzzle, we trapped and crystallized an intermediate in the disulphide hand-off, which showed a 165 degrees domain rotation relative to the original structure, bringing the two active sites within disulphide-bonding distance. The comparable structure of a mammalian QSOX enzyme, also presented here, shows further biochemical features that facilitate disulphide transfer in metazoan orthologues. Finally, we quantified the contribution of concatenation to QSOX activity, providing general lessons for the understanding of multi-domain enzymes and the design of new catalytic relays.

-	~~{{STRUCTURE_3qcp\| PDB=3qcp \| SCENE= }}~~	+	The dynamic disulphide relay of quiescin sulphydryl oxidase.,Alon A, Grossman I, Gat Y, Kodali VK, DiMaio F, Mehlman T, Haran G, Baker D, Thorpe C, Fass D Nature. 2012 Aug 16;488(7411):414-8. PMID:22801504<ref>PMID:22801504</ref>

-	~~===QSOX from Trypanosoma brucei===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22801504}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3qcp]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Trypanosoma_brucei Trypanosoma brucei]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022801504<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thiol oxidase]]		[[Category: Thiol oxidase]]
	[[Category: Trypanosoma brucei]]		[[Category: Trypanosoma brucei]]

OCA at 05:06, 22 August 2012

2012-08-22T05:06:53Z

←Older revision		Revision as of 05:06, 22 August 2012
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-	[[Image:3qcp.~~jpg~~\|left\|200px]]	+	[[Image:3qcp.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3qcp", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3qcp\| PDB=3qcp \| SCENE= }}		{{STRUCTURE_3qcp\| PDB=3qcp \| SCENE= }}

	===QSOX from Trypanosoma brucei===		===QSOX from Trypanosoma brucei===

		+	{{ABSTRACT_PUBMED_22801504}}

	==About this Structure==		==About this Structure==
	[[3qcp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA].		[[3qcp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022801504</ref><references group="xtra"/>
	[[Category: Thiol oxidase]]		[[Category: Thiol oxidase]]
	[[Category: Trypanosoma brucei]]		[[Category: Trypanosoma brucei]]

OCA at 05:58, 30 May 2012

2012-05-30T05:58:45Z

←Older revision		Revision as of 05:58, 30 May 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:3qcp.jpg\|left\|200px]]

-	The ~~entry 3qcp~~ is ~~ON HOLD~~ ~~until Jul 09 2013~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qcp", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qcp\| PDB=3qcp \| SCENE= }}

-	~~Authors: Alon, A., Fass, D.~~	+	===QSOX from Trypanosoma brucei===

-	~~Description~~: ~~QSOX~~ from Trypanosoma brucei	+
		+	==About this Structure==
		+	[[3qcp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QCP OCA].
		+	[[Category: Thiol oxidase]]
		+	[[Category: Trypanosoma brucei]]
		+	[[Category: Alon, A.]]
		+	[[Category: Fass, D.]]
		+	[[Category: Erv fold]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Sulfhydryl oxidase]]
		+	[[Category: Thioredoxin fold]]

OCA at 07:15, 18 April 2012

2012-04-18T07:15:59Z

←Older revision		Revision as of 07:15, 18 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qcp is ON HOLD until ~~Apr 18~~ 2013	+	The entry 3qcp is ON HOLD until Jul 09 2013

	Authors: Alon, A., Fass, D.		Authors: Alon, A., Fass, D.

	Description: QSOX from Trypanosoma brucei		Description: QSOX from Trypanosoma brucei

OCA at 05:36, 4 January 2012

2012-01-04T05:36:41Z

←Older revision		Revision as of 05:36, 4 January 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qcp is ON HOLD until ~~Jan 17~~ 2013	+	The entry 3qcp is ON HOLD until Apr 18 2013

	Authors: Alon, A., Fass, D.		Authors: Alon, A., Fass, D.

	Description: QSOX from Trypanosoma brucei		Description: QSOX from Trypanosoma brucei

OCA at 06:32, 28 December 2011

2011-12-28T06:32:56Z

←Older revision		Revision as of 06:32, 28 December 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qcp is ON HOLD until ~~Paper Publication~~	+	The entry 3qcp is ON HOLD until Jan 17 2013

	Authors: Alon, A., Fass, D.		Authors: Alon, A., Fass, D.

	Description: QSOX from Trypanosoma brucei		Description: QSOX from Trypanosoma brucei