3qui - Revision history

OCA at 11:58, 14 March 2024

2024-03-14T11:58:39Z

←Older revision		Revision as of 11:58, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3qui' size='340' side='right'caption='[[3qui]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='3qui' size='340' side='right'caption='[[3qui]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qui]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QUI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qui]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QUI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.933Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1u1t\|1u1t]], [[1u1s\|1u1s]], [[3m4g\|3m4g]], [[3inz\|3inz]], [[1hk9\|1hk9]], [[3gib\|3gib]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qui OCA], [https://pdbe.org/3qui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qui RCSB], [https://www.ebi.ac.uk/pdbsum/3qui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qui ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qui OCA], [https://pdbe.org/3qui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qui RCSB], [https://www.ebi.ac.uk/pdbsum/3qui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qui ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE]] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).	+	[https://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The Hfq protein forms a doughnut-shaped homohexamer that possesses RNA-binding activity. There are two distinct RNA-binding surfaces located on the proximal and the distal sides of the hexamer. The proximal side is involved in the binding of mRNA and small noncoding RNAs (sRNAs), while the distal side has an affinity for A-rich RNA sequences. In this work, the ability of various ribonucleotides to form complexes with Hfq from Pseudomonas aeruginosa has been tested using X-ray crystallography. ATP and ADPNP have been located in the distal R-site, which is a site for poly(A) RNA binding. UTP has been found in the so-called lateral RNA-binding site at the proximal surface. CTP has been found in both the distal R-site and the proximal U-binding site. GTP did not form a complex with Hfq under the conditions tested. The results have demonstrated the power of the crystallographic method for locating ribonucleotides and predicting single-stranded RNA-binding sites on the protein surface.	+
-		+
-	Hfq binds ribonucleotides in three different RNA-binding sites.,Murina V, Lekontseva N, Nikulin A Acta Crystallogr D Biol Crystallogr. 2013 Aug;69(Pt 8):1504-13. doi:, 10.1107/S090744491301010X. Epub 2013 Jul 18. PMID:23897473<ref>PMID:23897473</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3qui" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
-	*[[Protein Hfq\|Protein Hfq]]	+	*[[Protein Hfq 3D structures\|Protein Hfq 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Gabdulkhakov, A G~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Murina, V N~~]]	+	[[Category: Gabdulkhakov AG]]
-	[[Category: ~~Nikulin, A D~~]]	+	[[Category: Murina VN]]
-	[[Category: ~~Lsm]]~~	+	[[Category: Nikulin AD]]
-	~~[[Category: Mrna]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rna chaperone]]~~	+
-	~~[[Category: Rna-binding protein]]~~	+
-	~~[[Category: Srna~~]]	+

OCA at 05:36, 15 June 2022

2022-06-15T05:36:46Z

←Older revision		Revision as of 05:36, 15 June 2022
Line 1:		Line 1:

	==Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP==		==Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP==
-	<StructureSection load='3qui' size='340' side='right' caption='[[3qui]], [[Resolution\|resolution]] 1.93Å' scene=''>	+	<StructureSection load='3qui' size='340' side='right'caption='[[3qui]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qui]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QUI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qui]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QUI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u1t\|1u1t]], [[1u1s\|1u1s]], [[3m4g\|3m4g]], [[3inz\|3inz]], [[1hk9\|1hk9]], [[3gib\|3gib]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1u1t\|1u1t]], [[1u1s\|1u1s]], [[3m4g\|3m4g]], [[3inz\|3inz]], [[1hk9\|1hk9]], [[3gib\|3gib]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qui OCA], [~~http~~://pdbe.org/3qui PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qui RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qui PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qui ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qui OCA], [https://pdbe.org/3qui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qui RCSB], [https://www.ebi.ac.uk/pdbsum/3qui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qui ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE]] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).	+	[[https://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE]] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3qui" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3qui" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Protein Hfq\|Protein Hfq]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gabdulkhakov, A G]]		[[Category: Gabdulkhakov, A G]]
	[[Category: Murina, V N]]		[[Category: Murina, V N]]

OCA at 01:37, 5 August 2016

2016-08-05T01:37:52Z

←Older revision		Revision as of 01:37, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP==		==Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP==
	<StructureSection load='3qui' size='340' side='right' caption='[[3qui]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='3qui' size='340' side='right' caption='[[3qui]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qui]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QUI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qui]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QUI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u1t\|1u1t]], [[1u1s\|1u1s]], [[3m4g\|3m4g]], [[3inz\|3inz]], [[1hk9\|1hk9]], [[3gib\|3gib]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u1t\|1u1t]], [[1u1s\|1u1s]], [[3m4g\|3m4g]], [[3inz\|3inz]], [[1hk9\|1hk9]], [[3gib\|3gib]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 ~~Pseudomonas aeruginosa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qui OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qui RCSB], [http://www.ebi.ac.uk/pdbsum/3qui PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qui OCA], [http://pdbe.org/3qui PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qui RCSB], [http://www.ebi.ac.uk/pdbsum/3qui PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qui ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qui" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Gabdulkhakov, A G]]		[[Category: Gabdulkhakov, A G]]
	[[Category: Murina, V N]]		[[Category: Murina, V N]]

OCA at 14:46, 25 December 2014

2014-12-25T14:46:00Z

←Older revision		Revision as of 14:46, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3qui\| PDB=3qui \| SCENE= }}~~	+	==Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP==
-	===Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP===	+	<StructureSection load='3qui' size='340' side='right' caption='[[3qui]], [[Resolution\|resolution]] 1.93Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23897473}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3qui]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QUI FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u1t\|1u1t]], [[1u1s\|1u1s]], [[3m4g\|3m4g]], [[3inz\|3inz]], [[1hk9\|1hk9]], [[3gib\|3gib]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qui OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qui RCSB], [http://www.ebi.ac.uk/pdbsum/3qui PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE]] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).		[[http://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE]] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Hfq protein forms a doughnut-shaped homohexamer that possesses RNA-binding activity. There are two distinct RNA-binding surfaces located on the proximal and the distal sides of the hexamer. The proximal side is involved in the binding of mRNA and small noncoding RNAs (sRNAs), while the distal side has an affinity for A-rich RNA sequences. In this work, the ability of various ribonucleotides to form complexes with Hfq from Pseudomonas aeruginosa has been tested using X-ray crystallography. ATP and ADPNP have been located in the distal R-site, which is a site for poly(A) RNA binding. UTP has been found in the so-called lateral RNA-binding site at the proximal surface. CTP has been found in both the distal R-site and the proximal U-binding site. GTP did not form a complex with Hfq under the conditions tested. The results have demonstrated the power of the crystallographic method for locating ribonucleotides and predicting single-stranded RNA-binding sites on the protein surface.

-	~~==About this Structure==~~	+	Hfq binds ribonucleotides in three different RNA-binding sites.,Murina V, Lekontseva N, Nikulin A Acta Crystallogr D Biol Crystallogr. 2013 Aug;69(Pt 8):1504-13. doi:, 10.1107/S090744491301010X. Epub 2013 Jul 18. PMID:23897473<ref>PMID:23897473</ref>
-	~~[[3qui]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3QUI OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023897473</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Gabdulkhakov, A G.]]	+	[[Category: Gabdulkhakov, A G]]
-	[[Category: Murina, V N.]]	+	[[Category: Murina, V N]]
-	[[Category: Nikulin, A D.]]	+	[[Category: Nikulin, A D]]
	[[Category: Lsm]]		[[Category: Lsm]]
	[[Category: Mrna]]		[[Category: Mrna]]

OCA at 05:34, 28 August 2013

2013-08-28T05:34:58Z

←Older revision		Revision as of 05:34, 28 August 2013
Line 1:		Line 1:
-	[[Image:3qui.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3qui", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3qui\| PDB=3qui \| SCENE= }}		{{STRUCTURE_3qui\| PDB=3qui \| SCENE= }}
-
	===Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP===		===Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP===
		+	{{ABSTRACT_PUBMED_23897473}}

		+	==Function==
		+	[[http://www.uniprot.org/uniprot/HFQ_PSEAE HFQ_PSEAE]] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs (By similarity).

	==About this Structure==		==About this Structure==
	[[3qui]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA].		[[3qui]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023897473</ref><references group="xtra"/><references/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Gabdulkhakov, A G.]]		[[Category: Gabdulkhakov, A G.]]

OCA at 06:38, 8 February 2012

2012-02-08T06:38:12Z

←Older revision		Revision as of 06:38, 8 February 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3qui.jpg\|left\|200px]]

-	The ~~entry 3qui~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qui", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qui\| PDB=3qui \| SCENE= }}

-	~~Authors: Nikulin, A.D., Murina, V.N., Gabdulkhakov, A.G.~~	+	===Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP===

-	~~Description: Crystal~~ structure of Pseudomonas aeruginosa ~~Hfq in complex with ADPNP~~	+
		+	==About this Structure==
		+	[[3qui]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUI OCA].
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Gabdulkhakov, A G.]]
		+	[[Category: Murina, V N.]]
		+	[[Category: Nikulin, A D.]]
		+	[[Category: Lsm]]
		+	[[Category: Mrna]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Rna chaperone]]
		+	[[Category: Rna-binding protein]]
		+	[[Category: Srna]]

OCA at 06:07, 9 March 2011

2011-03-09T06:07:16Z

←Older revision		Revision as of 06:07, 9 March 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qui is ON HOLD	+	The entry 3qui is ON HOLD until Paper Publication

	Authors: Nikulin, A.D., Murina, V.N., Gabdulkhakov, A.G.		Authors: Nikulin, A.D., Murina, V.N., Gabdulkhakov, A.G.

	Description: Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP		Description: Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP

OCA: Protected "3qui" [edit=sysop:move=sysop]

2011-03-02T06:45:23Z

Protected "3qui" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:45, 2 March 2011

OCA: New page: '''Unreleased structure''' The entry 3qui is ON HOLD Authors: Nikulin, A.D., Murina, V.N., Gabdulkhakov, A.G. Description: Crystal structure of Pseudomonas aeruginosa Hfq in complex wi...

2011-03-02T06:45:21Z

New page: '''Unreleased structure''' The entry 3qui is ON HOLD Authors: Nikulin, A.D., Murina, V.N., Gabdulkhakov, A.G. Description: Crystal structure of Pseudomonas aeruginosa Hfq in complex wi...

New page

'''Unreleased structure'''

The entry 3qui is ON HOLD

Authors: Nikulin, A.D., Murina, V.N., Gabdulkhakov, A.G.

Description: Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP