3qxq - Revision history

OCA at 10:50, 21 February 2024

2024-02-21T10:50:39Z

←Older revision		Revision as of 10:50, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3qxq' size='340' side='right'caption='[[3qxq]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3qxq' size='340' side='right'caption='[[3qxq]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qxq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QXQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qxq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QXQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BGC:BETA~~-~~D-GLUCOSE'>BGC</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3qxf\|3qxf]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PRD_900016:beta-cellopentaose'>PRD_900016</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>bcsZ, bcsC, yhjM, b3531, JW3499 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~83333 ECOLI])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qxq OCA], [https://pdbe.org/3qxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qxq RCSB], [https://www.ebi.ac.uk/pdbsum/3qxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qxq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qxq OCA], [https://pdbe.org/3qxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qxq RCSB], [https://www.ebi.ac.uk/pdbsum/3qxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qxq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GUN_ECOLI GUN_ECOLI]] Hydrolyzes carboxymethylcellulose.	+	[https://www.uniprot.org/uniprot/GUN_ECOLI GUN_ECOLI] Hydrolyzes carboxymethylcellulose.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Cellulose, a very abundant extracellular polysaccharide, is synthesized in a finely tuned process that involves the activity of glycosyl-transferases and hydrolases. The cellulose microfibril consists of bundles of linear beta-1,4-glucan chains that are synthesized inside the cell; however, the mechanism by which these polymers traverse the cell membrane is currently unknown. In Gram-negative bacteria, the cellulose synthase complex forms a trans-envelope complex consisting of at least four subunits. Although three of these subunits account for the synthesis and translocation of the polysaccharide, the fourth subunit, BcsZ, is a periplasmic protein with endo-beta-1,4-glucanase activity. BcsZ belongs to family eight of glycosyl-hydrolases, and its activity is required for optimal synthesis and membrane translocation of cellulose. In this study we report two crystal structures of BcsZ from Escherichia coli. One structure shows the wild-type enzyme in its apo form, and the second structure is for a catalytically inactive mutant of BcsZ in complex with the substrate cellopentaose. The structures demonstrate that BcsZ adopts an (alpha/alpha)(6)-barrel fold and that it binds four glucan moieties of cellopentaose via highly conserved residues exclusively on the nonreducing side of its catalytic center. Thus, the BcsZ-cellopentaose structure most likely represents a posthydrolysis state in which the newly formed nonreducing end has already left the substrate binding pocket while the enzyme remains attached to the truncated polysaccharide chain. We further show that BcsZ efficiently degrades beta-1,4-glucans in in vitro cellulase assays with carboxymethyl-cellulose as substrate.	+
-		+
-	Apo- and cellopentaose-bound structures of the bacterial cellulose synthase subunit BcsZ.,Mazur O, Zimmer J J Biol Chem. 2011 May 20;286(20):17601-6. Epub 2011 Mar 25. PMID:21454578<ref>PMID:21454578</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3qxq" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cellulase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Zimmer, J]]	+	[[Category: Zimmer J]]
-	~~[[Category: Cellulose synthesis]]~~	+
-	~~[[Category: Gh8 glycoside hydrolase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 05:40, 15 June 2022

2022-06-15T05:40:44Z

←Older revision		Revision as of 05:40, 15 June 2022
Line 1:		Line 1:

	==Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose==		==Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose==
-	<StructureSection load='3qxq' size='340' side='right' caption='[[3qxq]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='3qxq' size='340' side='right'caption='[[3qxq]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qxq]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QXQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qxq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QXQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~CE5~~:~~CELLOPENTAOSE~~'>~~CE5~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qxf\|3qxf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qxf\|3qxf]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bcsZ, bcsC, yhjM, b3531, JW3499 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bcsZ, bcsC, yhjM, b3531, JW3499 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qxq OCA], [~~http~~://pdbe.org/3qxq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qxq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qxq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qxq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qxq OCA], [https://pdbe.org/3qxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qxq RCSB], [https://www.ebi.ac.uk/pdbsum/3qxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qxq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GUN_ECOLI GUN_ECOLI]] Hydrolyzes carboxymethylcellulose.	+	[[https://www.uniprot.org/uniprot/GUN_ECOLI GUN_ECOLI]] Hydrolyzes carboxymethylcellulose.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Glucanase\|Glucanase]]	+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Zimmer, J]]		[[Category: Zimmer, J]]
	[[Category: Cellulose synthesis]]		[[Category: Cellulose synthesis]]
	[[Category: Gh8 glycoside hydrolase]]		[[Category: Gh8 glycoside hydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 23:36, 4 August 2016

2016-08-04T23:36:05Z

←Older revision		Revision as of 23:36, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose==		==Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose==
	<StructureSection load='3qxq' size='340' side='right' caption='[[3qxq]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3qxq' size='340' side='right' caption='[[3qxq]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qxq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QXQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qxq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QXQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qxf\|3qxf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qxf\|3qxf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bcsZ, bcsC, yhjM, b3531, JW3499 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bcsZ, bcsC, yhjM, b3531, JW3499 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qxq RCSB], [http://www.ebi.ac.uk/pdbsum/3qxq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qxq OCA], [http://pdbe.org/3qxq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qxq RCSB], [http://www.ebi.ac.uk/pdbsum/3qxq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qxq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qxq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Cellulase]]		[[Category: Cellulase]]
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Zimmer, J]]		[[Category: Zimmer, J]]
	[[Category: Cellulose synthesis]]		[[Category: Cellulose synthesis]]
	[[Category: Gh8 glycoside hydrolase]]		[[Category: Gh8 glycoside hydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 20:45, 24 December 2014

2014-12-24T20:45:44Z

←Older revision		Revision as of 20:45, 24 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qxq RCSB], [http://www.ebi.ac.uk/pdbsum/3qxq PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qxq RCSB], [http://www.ebi.ac.uk/pdbsum/3qxq PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GUN_ECOLI GUN_ECOLI]] Hydrolyzes carboxymethylcellulose.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 13:32, 9 December 2014

2014-12-09T13:32:50Z

←Older revision		Revision as of 13:32, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3qxq.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose==
		+	<StructureSection load='3qxq' size='340' side='right' caption='[[3qxq]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3qxq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QXQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qxf\|3qxf]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bcsZ, bcsC, yhjM, b3531, JW3499 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qxq RCSB], [http://www.ebi.ac.uk/pdbsum/3qxq PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cellulose, a very abundant extracellular polysaccharide, is synthesized in a finely tuned process that involves the activity of glycosyl-transferases and hydrolases. The cellulose microfibril consists of bundles of linear beta-1,4-glucan chains that are synthesized inside the cell; however, the mechanism by which these polymers traverse the cell membrane is currently unknown. In Gram-negative bacteria, the cellulose synthase complex forms a trans-envelope complex consisting of at least four subunits. Although three of these subunits account for the synthesis and translocation of the polysaccharide, the fourth subunit, BcsZ, is a periplasmic protein with endo-beta-1,4-glucanase activity. BcsZ belongs to family eight of glycosyl-hydrolases, and its activity is required for optimal synthesis and membrane translocation of cellulose. In this study we report two crystal structures of BcsZ from Escherichia coli. One structure shows the wild-type enzyme in its apo form, and the second structure is for a catalytically inactive mutant of BcsZ in complex with the substrate cellopentaose. The structures demonstrate that BcsZ adopts an (alpha/alpha)(6)-barrel fold and that it binds four glucan moieties of cellopentaose via highly conserved residues exclusively on the nonreducing side of its catalytic center. Thus, the BcsZ-cellopentaose structure most likely represents a posthydrolysis state in which the newly formed nonreducing end has already left the substrate binding pocket while the enzyme remains attached to the truncated polysaccharide chain. We further show that BcsZ efficiently degrades beta-1,4-glucans in in vitro cellulase assays with carboxymethyl-cellulose as substrate.

-	~~{{STRUCTURE_3qxq\| PDB=3qxq \| SCENE= }}~~	+	Apo- and cellopentaose-bound structures of the bacterial cellulose synthase subunit BcsZ.,Mazur O, Zimmer J J Biol Chem. 2011 May 20;286(20):17601-6. Epub 2011 Mar 25. PMID:21454578<ref>PMID:21454578</ref>

-	~~===Structure~~ of the ~~bacterial cellulose synthase subunit Z in complex with cellopentaose===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21454578}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3qxq]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA].~~	+

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase\|Glucanase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021454578<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Zimmer, J.]]	+	[[Category: Zimmer, J]]
	[[Category: Cellulose synthesis]]		[[Category: Cellulose synthesis]]
	[[Category: Gh8 glycoside hydrolase]]		[[Category: Gh8 glycoside hydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 08:58, 20 October 2012

2012-10-20T08:58:28Z

←Older revision		Revision as of 08:58, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3qxq]] is a 4 chain structure ~~of [[Glucanase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA].	+	[[3qxq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA].

	==See Also==		==See Also==

OCA at 21:22, 25 July 2012

2012-07-25T21:22:35Z

←Older revision		Revision as of 21:22, 25 July 2012
Line 1:		Line 1:
	[[Image:3qxq.png\|left\|200px]]		[[Image:3qxq.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3qxq", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3qxq\| PDB=3qxq \| SCENE= }}		{{STRUCTURE_3qxq\| PDB=3qxq \| SCENE= }}

	===Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose===		===Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21454578}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21454578 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21454578}}		{{ABSTRACT_PUBMED_21454578}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Glucanase]]	+	*[[Glucanase\|Glucanase]]

	==Reference==		==Reference==

OCA at 05:02, 10 August 2011

2011-08-10T05:02:15Z

←Older revision		Revision as of 05:02, 10 August 2011
Line 1:		Line 1:
-	[[Image:3qxq.~~jpg~~\|left\|200px]]	+	[[Image:3qxq.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose===		===Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21454578}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21454578 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21454578}}

	==About this Structure==		==About this Structure==
-	[[3qxq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA].	+	[[3qxq]] is a 4 chain structure of [[Glucanase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA].
		+
		+	==See Also==
		+	*[[Glucanase]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021454578</ref><references group="xtra"/>
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Zimmer, J.]]		[[Category: Zimmer, J.]]
		+	[[Category: Cellulose synthesis]]
		+	[[Category: Gh8 glycoside hydrolase]]
		+	[[Category: Hydrolase]]

OCA at 08:18, 30 March 2011

2011-03-30T08:18:25Z

←Older revision		Revision as of 08:18, 30 March 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3qxq.jpg\|left\|200px]]

-	The ~~entry 3qxq~~ is ~~ON HOLD until sometime in~~ the ~~future~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qxq", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qxq\| PDB=3qxq \| SCENE= }}

-	~~Authors: Zimmer, J.~~	+	===Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose===

-	~~Description:~~ Structure ~~of the bacterial cellulose synthase subunit Z in complex~~ with ~~cellopentaose~~	+
		+	==About this Structure==
		+	[[3qxq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QXQ OCA].
		+	[[Category: Cellulase]]
		+	[[Category: Escherichia coli]]
		+	[[Category: Zimmer, J.]]

OCA at 08:05, 16 March 2011

2011-03-16T08:05:05Z

←Older revision		Revision as of 08:05, 16 March 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qxq is ON HOLD	+	The entry 3qxq is ON HOLD until sometime in the future

	Authors: Zimmer, J.		Authors: Zimmer, J.

	Description: Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose		Description: Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose