3rcc - Revision history

OCA at 10:53, 21 February 2024

2024-02-21T10:53:43Z

←Older revision		Revision as of 10:53, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3rcc' size='340' side='right'caption='[[3rcc]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='3rcc' size='340' side='right'caption='[[3rcc]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rcc]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Streptococcus_agalactiae_(serotype_v)~~ Streptococcus agalactiae ~~(serotype v)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RCC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rcc]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_V Streptococcus agalactiae serogroup V]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RCC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3rbi\|3rbi]], [[3rbj\|3rbj]], [[3rbk\|3rbk]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">SAG0961, srtA, srtA (SAG0961) ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae (serotype V)])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcc OCA], [https://pdbe.org/3rcc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rcc RCSB], [https://www.ebi.ac.uk/pdbsum/3rcc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rcc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcc OCA], [https://pdbe.org/3rcc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rcc RCSB], [https://www.ebi.ac.uk/pdbsum/3rcc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rcc ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q8DZY1_STRA5 Q8DZY1_STRA5]
-	The assembly of pili on the cell wall of Gram-positive bacteria requires transpeptidase enzymes called sortases. In Streptococcus agalactiae, the PI-1 pilus island of strain 2603V/R encodes two pilus-specific sortases (SrtC1 and SrtC2) and three pilins (GBS80, GBS52 and GBS104). Although either pilus-specific sortase is sufficient for the polymerization of the major pilin, GBS80, incorporation of the minor pilins GBS52 and GBS104 into the pilus structure requires SrtC1 and SrtC2, respectively. The S. agalactiae housekeeping sortase, SrtA, whose gene is present at a different location and does not catalyze pilus polymerization, was shown to be involved in cell wall anchoring of pilus polymers. To understand the structural basis of sortases involved in such diverse functions, we determined the crystal structures of S. agalactiae SrtC1 and SrtA. Both enzymes are made of an eight-stranded beta-barrel core with variations in their active site architecture. SrtA exhibits a catalytic triad arrangement similar to that in Streptococcus pyogenes SrtA but different from that in Staphylococcus aureus SrtA. In contrast, the SrtC1 enzyme contains an N-terminal helical domain and a 'lid' in its putative active site, which is similar to that seen in Streptococcus pneumoniae pilus-specific sortases, although with subtle differences in positioning and composition. To understand the effect of such differences on substrate recognition, we have also determined the crystal structure of a SrtC1 mutant, in which the conserved DP(W/F/Y) motif was replaced with the sorting signal motif of GBS80, IPNTG. By comparing the structures of WT wild type SrtA and SrtC1 and the 'lid' mutant of SrtC1, we propose that structural elements within the active site and the lid may be important for defining the role of specific sortase in pili biogenesis.	+
-		+
-	~~Structural differences between the Streptococcus agalactiae housekeeping and pilus-specific sortases~~: ~~SrtA and SrtC1.,Khare B, Krishnan V, Rajashankar KR, I-Hsiu H, Xin M, Ton-That H, Narayana SV PLoS One. 2011;6(8):e22995. Epub 2011 Aug 30. PMID:21912586<ref>PMID:21912586<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3rcc" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Khare, B]]~~	+	[[Category: Streptococcus agalactiae serogroup V]]
-	~~[[Category: Narayana, S~~ V.L]]	+	[[Category: Khare B]]
-	[[Category: ~~Beta-barrel~~]]	+	[[Category: Narayana SVL]]
-	[[Category: ~~Housekeeping sortase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Pili anchoring]]~~	+
-	~~[[Category: Pili biogenesis]]~~	+
-	~~[[Category: Sortase fold]]~~	+
-	~~[[Category: Surface protein anchoring~~]]	+

OCA at 05:57, 15 June 2022

2022-06-15T05:57:44Z

←Older revision		Revision as of 05:57, 15 June 2022
Line 1:		Line 1:

	==Crystal Structure of the Streptococcus agalactiae Sortase A==		==Crystal Structure of the Streptococcus agalactiae Sortase A==
-	<StructureSection load='3rcc' size='340' side='right' caption='[[3rcc]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='3rcc' size='340' side='right'caption='[[3rcc]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rcc]] is a 18 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptococcus_agalactiae_(serotype_v) Streptococcus agalactiae (serotype v)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RCC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rcc]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_(serotype_v) Streptococcus agalactiae (serotype v)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RCC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbi\|3rbi]], [[3rbj\|3rbj]], [[3rbk\|3rbk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rbi\|3rbi]], [[3rbj\|3rbj]], [[3rbk\|3rbk]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG0961, srtA, srtA (SAG0961) ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae (serotype V)])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG0961, srtA, srtA (SAG0961) ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae (serotype V)])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcc OCA], [~~http~~://pdbe.org/3rcc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rcc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rcc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rcc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcc OCA], [https://pdbe.org/3rcc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rcc RCSB], [https://www.ebi.ac.uk/pdbsum/3rcc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rcc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Khare, B]]		[[Category: Khare, B]]
	[[Category: Narayana, S V.L]]		[[Category: Narayana, S V.L]]

OCA at 13:32, 11 August 2016

2016-08-11T13:32:26Z

←Older revision		Revision as of 13:32, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of the Streptococcus agalactiae Sortase A==		==Crystal Structure of the Streptococcus agalactiae Sortase A==
	<StructureSection load='3rcc' size='340' side='right' caption='[[3rcc]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='3rcc' size='340' side='right' caption='[[3rcc]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rcc]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_agalactiae_serogroup_v~~ Streptococcus agalactiae ~~serogroup~~ v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RCC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rcc]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_(serotype_v) Streptococcus agalactiae (serotype v)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RCC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbi\|3rbi]], [[3rbj\|3rbj]], [[3rbk\|3rbk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbi\|3rbi]], [[3rbj\|3rbj]], [[3rbk\|3rbk]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG0961, srtA, srtA (SAG0961) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae ~~serogroup~~ V])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG0961, srtA, srtA (SAG0961) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae (serotype V)])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rcc RCSB], [http://www.ebi.ac.uk/pdbsum/3rcc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcc OCA], [http://pdbe.org/3rcc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rcc RCSB], [http://www.ebi.ac.uk/pdbsum/3rcc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rcc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rcc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptococcus agalactiae serogroup v]]
	[[Category: Khare, B]]		[[Category: Khare, B]]
	[[Category: Narayana, S V.L]]		[[Category: Narayana, S V.L]]

OCA at 10:31, 4 January 2015

2015-01-04T10:31:08Z

←Older revision		Revision as of 10:31, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rcc]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RCC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rcc]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RCC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbi\|3rbi]], [[3rbj\|3rbj]], [[3rbk\|3rbk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbi\|3rbi]], [[3rbj\|3rbj]], [[3rbk\|3rbk]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG0961, srtA, srtA (SAG0961) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae serogroup V])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG0961, srtA, srtA (SAG0961) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae serogroup V])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rcc RCSB], [http://www.ebi.ac.uk/pdbsum/3rcc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rcc RCSB], [http://www.ebi.ac.uk/pdbsum/3rcc PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Structural differences between the Streptococcus agalactiae housekeeping and pilus-specific sortases: SrtA and SrtC1.,Khare B, Krishnan V, Rajashankar KR, I-Hsiu H, Xin M, Ton-That H, Narayana SV PLoS One. 2011;6(8):e22995. Epub 2011 Aug 30. PMID:21912586<ref>PMID:21912586</ref>		Structural differences between the Streptococcus agalactiae housekeeping and pilus-specific sortases: SrtA and SrtC1.,Khare B, Krishnan V, Rajashankar KR, I-Hsiu H, Xin M, Ton-That H, Narayana SV PLoS One. 2011;6(8):e22995. Epub 2011 Aug 30. PMID:21912586<ref>PMID:21912586</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Streptococcus agalactiae serogroup v]]		[[Category: Streptococcus agalactiae serogroup v]]
-	[[Category: Khare, B.]]	+	[[Category: Khare, B]]
-	[[Category: Narayana, S V.L.]]	+	[[Category: Narayana, S V.L]]
	[[Category: Beta-barrel]]		[[Category: Beta-barrel]]
	[[Category: Housekeeping sortase]]		[[Category: Housekeeping sortase]]

OCA at 04:50, 5 June 2014

2014-06-05T04:50:48Z

←Older revision		Revision as of 04:50, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3rcc.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of the Streptococcus agalactiae Sortase A==
		+	<StructureSection load='3rcc' size='340' side='right' caption='[[3rcc]], [[Resolution\|resolution]] 3.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rcc]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RCC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbi\|3rbi]], [[3rbj\|3rbj]], [[3rbk\|3rbk]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAG0961, srtA, srtA (SAG0961) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae serogroup V])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rcc RCSB], [http://www.ebi.ac.uk/pdbsum/3rcc PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The assembly of pili on the cell wall of Gram-positive bacteria requires transpeptidase enzymes called sortases. In Streptococcus agalactiae, the PI-1 pilus island of strain 2603V/R encodes two pilus-specific sortases (SrtC1 and SrtC2) and three pilins (GBS80, GBS52 and GBS104). Although either pilus-specific sortase is sufficient for the polymerization of the major pilin, GBS80, incorporation of the minor pilins GBS52 and GBS104 into the pilus structure requires SrtC1 and SrtC2, respectively. The S. agalactiae housekeeping sortase, SrtA, whose gene is present at a different location and does not catalyze pilus polymerization, was shown to be involved in cell wall anchoring of pilus polymers. To understand the structural basis of sortases involved in such diverse functions, we determined the crystal structures of S. agalactiae SrtC1 and SrtA. Both enzymes are made of an eight-stranded beta-barrel core with variations in their active site architecture. SrtA exhibits a catalytic triad arrangement similar to that in Streptococcus pyogenes SrtA but different from that in Staphylococcus aureus SrtA. In contrast, the SrtC1 enzyme contains an N-terminal helical domain and a 'lid' in its putative active site, which is similar to that seen in Streptococcus pneumoniae pilus-specific sortases, although with subtle differences in positioning and composition. To understand the effect of such differences on substrate recognition, we have also determined the crystal structure of a SrtC1 mutant, in which the conserved DP(W/F/Y) motif was replaced with the sorting signal motif of GBS80, IPNTG. By comparing the structures of WT wild type SrtA and SrtC1 and the 'lid' mutant of SrtC1, we propose that structural elements within the active site and the lid may be important for defining the role of specific sortase in pili biogenesis.

-	<!--	+	Structural differences between the Streptococcus agalactiae housekeeping and pilus-specific sortases: SrtA and SrtC1.,Khare B, Krishnan V, Rajashankar KR, I-Hsiu H, Xin M, Ton-That H, Narayana SV PLoS One. 2011;6(8):e22995. Epub 2011 Aug 30. PMID:21912586<ref>PMID:21912586</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3rcc"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3rcc\| PDB=3rcc \| SCENE= }}~~	+

-	~~===Crystal Structure~~ of the ~~Streptococcus agalactiae Sortase A===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21912586}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21912586 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21912586}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rcc]] is a 18 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021912586<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptococcus agalactiae serogroup v]]		[[Category: Streptococcus agalactiae serogroup v]]
	[[Category: Khare, B.]]		[[Category: Khare, B.]]

OCA at 06:51, 28 March 2012

2012-03-28T06:51:04Z

←Older revision		Revision as of 06:51, 28 March 2012
Line 1:		Line 1:
-	[[Image:3rcc.~~jpg~~\|left\|200px]]	+	[[Image:3rcc.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal Structure of the Streptococcus agalactiae Sortase A===		===Crystal Structure of the Streptococcus agalactiae Sortase A===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21912586}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21912586 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21912586}}

	==About this Structure==		==About this Structure==
	[[3rcc]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA].		[[3rcc]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021912586</ref><references group="xtra"/>
	[[Category: Streptococcus agalactiae serogroup v]]		[[Category: Streptococcus agalactiae serogroup v]]
	[[Category: Khare, B.]]		[[Category: Khare, B.]]

OCA at 06:19, 7 September 2011

2011-09-07T06:19:22Z

←Older revision		Revision as of 06:19, 7 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rcc.jpg\|left\|200px]]

-	The ~~entry 3rcc~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rcc", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rcc\| PDB=3rcc \| SCENE= }}

-	~~Authors: Khare, B., Narayana, S.V.L.~~	+	===Crystal Structure of the Streptococcus agalactiae Sortase A===

-	~~Description: Crystal~~ Structure ~~of the~~ Streptococcus agalactiae Sortase A	+
		+	==About this Structure==
		+	[[3rcc]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_v Streptococcus agalactiae serogroup v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCC OCA].
		+	[[Category: Streptococcus agalactiae serogroup v]]
		+	[[Category: Khare, B.]]
		+	[[Category: Narayana, S V.L.]]
		+	[[Category: Beta-barrel]]
		+	[[Category: Housekeeping sortase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Pili anchoring]]
		+	[[Category: Pili biogenesis]]
		+	[[Category: Sortase fold]]
		+	[[Category: Surface protein anchoring]]

OCA at 06:30, 4 May 2011

2011-05-04T06:30:27Z

←Older revision		Revision as of 06:30, 4 May 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rcc is ON HOLD	+	The entry 3rcc is ON HOLD until Paper Publication

	Authors: Khare, B., Narayana, S.V.L.		Authors: Khare, B., Narayana, S.V.L.

	Description: Crystal Structure of the Streptococcus agalactiae Sortase A		Description: Crystal Structure of the Streptococcus agalactiae Sortase A

OCA: Protected "3rcc" [edit=sysop:move=sysop]

2011-04-06T08:43:30Z

Protected "3rcc" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:43, 6 April 2011

OCA: New page: '''Unreleased structure''' The entry 3rcc is ON HOLD Authors: Khare, B., Narayana, S.V.L. Description: Crystal Structure of the Streptococcus agalactiae Sortase A

2011-04-06T08:43:29Z

New page: '''Unreleased structure''' The entry 3rcc is ON HOLD Authors: Khare, B., Narayana, S.V.L. Description: Crystal Structure of the Streptococcus agalactiae Sortase A

New page

'''Unreleased structure'''

The entry 3rcc is ON HOLD

Authors: Khare, B., Narayana, S.V.L.

Description: Crystal Structure of the Streptococcus agalactiae Sortase A