3rdx - Revision history

OCA at 12:20, 14 March 2024

2024-03-14T12:20:07Z

←Older revision		Revision as of 12:20, 14 March 2024
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	<StructureSection load='3rdx' size='340' side='right'caption='[[3rdx]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3rdx' size='340' side='right'caption='[[3rdx]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rdx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RDX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rdx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RDX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.101Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3rdm\|3rdm]], [[3rdo\|3rdo]], [[3rdq\|3rdq]], [[3rds\|3rds]], [[3rdu\|3rdu]], [[3re5\|3re5]], [[3re6\|3re6]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdx OCA], [https://pdbe.org/3rdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rdx RCSB], [https://www.ebi.ac.uk/pdbsum/3rdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rdx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdx OCA], [https://pdbe.org/3rdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rdx RCSB], [https://www.ebi.ac.uk/pdbsum/3rdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rdx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	We have performed a detailed analysis of streptavidin variants with altered specificity towards desthiobiotin. In addition to changes in key residues which widen the ligand binding pocket and accommodate the more structurally flexible desthiobiotin, the data revealed the role of a key, non-active site mutation at the base of the flexible loop (S52G) which slows dissociation of this ligand by approximately sevenfold. Our data suggest that this mutation results in the loss of a stabilizing contact which keeps this loop open and accessible in the absence of ligand. When this mutation was introduced into the wild-type protein, destabilization of the opened loop conferred a approximately 10-fold decrease in both the on-rate and off-rate for the ligand biotin-4-fluoroscein. A similar effect was observed when this mutation was added to a monomeric form of this protein. Our results provide key insight into the role of the streptavidin flexible loop in ligand binding and maintaining high affinity interactions.	+
-		+
-	Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding.,Magalhaes ML, Czekster CM, Guan R, Malashkevich VN, Almo SC, Levy M Protein Sci. 2011 Jul;20(7):1145-54. doi: 10.1002/pro.642. Epub 2011 May, 12. PMID:21520321<ref>PMID:21520321</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3rdx" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Almo~~, S C~~]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: Czecster~~, C M~~]]	+	[[Category: Almo SC]]
-	[[Category: Guan, R]]	+	[[Category: Czecster CM]]
-	[[Category: Levy, M]]	+	[[Category: Guan R]]
-	[[Category: Magalhaes, M]]	+	[[Category: Levy M]]
-	[[Category: Malashkevich~~, V N]]~~	+	[[Category: Magalhaes M]]
-	~~[[Category: Biotin binding protein]]~~	+	[[Category: Malashkevich VN]]
-	~~[[Category: Improved desthiobiotin binding]]~~	+
-	~~[[Category: Opened loop destabilization]]~~	+
-	~~[[Category: Streptavidin variant~~]]	+

OCA at 05:59, 15 June 2022

2022-06-15T05:59:23Z

←Older revision		Revision as of 05:59, 15 June 2022
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	<StructureSection load='3rdx' size='340' side='right'caption='[[3rdx]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3rdx' size='340' side='right'caption='[[3rdx]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rdx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RDX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rdx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RDX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rdm\|3rdm]], [[3rdo\|3rdo]], [[3rdq\|3rdq]], [[3rds\|3rds]], [[3rdu\|3rdu]], [[3re5\|3re5]], [[3re6\|3re6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rdm\|3rdm]], [[3rdo\|3rdo]], [[3rdq\|3rdq]], [[3rds\|3rds]], [[3rdu\|3rdu]], [[3re5\|3re5]], [[3re6\|3re6]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdx OCA], [~~http~~://pdbe.org/3rdx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rdx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rdx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rdx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdx OCA], [https://pdbe.org/3rdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rdx RCSB], [https://www.ebi.ac.uk/pdbsum/3rdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rdx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 14:15, 25 December 2019

2019-12-25T14:15:15Z

←Older revision		Revision as of 14:15, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of ligand-free R7-2 streptavidin==		==Crystal structure of ligand-free R7-2 streptavidin==
-	<StructureSection load='3rdx' size='340' side='right' caption='[[3rdx]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3rdx' size='340' side='right'caption='[[3rdx]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RDX FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RDX FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3rdx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3rdx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]
	[[Category: Czecster, C M]]		[[Category: Czecster, C M]]

OCA at 19:34, 24 January 2018

2018-01-24T19:34:45Z

←Older revision		Revision as of 19:34, 24 January 2018
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3rdx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3rdx" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Avidin\|Avidin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:26, 5 August 2016

2016-08-05T06:26:09Z

←Older revision		Revision as of 06:26, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of ligand-free R7-2 streptavidin==		==Crystal structure of ligand-free R7-2 streptavidin==
	<StructureSection load='3rdx' size='340' side='right' caption='[[3rdx]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3rdx' size='340' side='right' caption='[[3rdx]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_avidinii Streptomyces avidinii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RDX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RDX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rdm\|3rdm]], [[3rdo\|3rdo]], [[3rdq\|3rdq]], [[3rds\|3rds]], [[3rdu\|3rdu]], [[3re5\|3re5]], [[3re6\|3re6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rdm\|3rdm]], [[3rdo\|3rdo]], [[3rdq\|3rdq]], [[3rds\|3rds]], [[3rdu\|3rdu]], [[3re5\|3re5]], [[3re6\|3re6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rdx RCSB], [http://www.ebi.ac.uk/pdbsum/3rdx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdx OCA], [http://pdbe.org/3rdx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rdx RCSB], [http://www.ebi.ac.uk/pdbsum/3rdx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rdx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rdx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces avidinii~~]]	+	[[Category: As 4 1583]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]
	[[Category: Czecster, C M]]		[[Category: Czecster, C M]]

OCA at 16:30, 25 December 2014

2014-12-25T16:30:27Z

←Older revision		Revision as of 16:30, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rdx RCSB], [http://www.ebi.ac.uk/pdbsum/3rdx PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rdx RCSB], [http://www.ebi.ac.uk/pdbsum/3rdx PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:14, 9 December 2014

2014-12-09T12:14:49Z

←Older revision		Revision as of 12:14, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3rdx.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of ligand-free R7-2 streptavidin==
		+	<StructureSection load='3rdx' size='340' side='right' caption='[[3rdx]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RDX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rdm\|3rdm]], [[3rdo\|3rdo]], [[3rdq\|3rdq]], [[3rds\|3rds]], [[3rdu\|3rdu]], [[3re5\|3re5]], [[3re6\|3re6]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rdx RCSB], [http://www.ebi.ac.uk/pdbsum/3rdx PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have performed a detailed analysis of streptavidin variants with altered specificity towards desthiobiotin. In addition to changes in key residues which widen the ligand binding pocket and accommodate the more structurally flexible desthiobiotin, the data revealed the role of a key, non-active site mutation at the base of the flexible loop (S52G) which slows dissociation of this ligand by approximately sevenfold. Our data suggest that this mutation results in the loss of a stabilizing contact which keeps this loop open and accessible in the absence of ligand. When this mutation was introduced into the wild-type protein, destabilization of the opened loop conferred a approximately 10-fold decrease in both the on-rate and off-rate for the ligand biotin-4-fluoroscein. A similar effect was observed when this mutation was added to a monomeric form of this protein. Our results provide key insight into the role of the streptavidin flexible loop in ligand binding and maintaining high affinity interactions.

-	~~{{STRUCTURE_3rdx\| PDB=3rdx \| SCENE= }}~~	+	Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding.,Magalhaes ML, Czekster CM, Guan R, Malashkevich VN, Almo SC, Levy M Protein Sci. 2011 Jul;20(7):1145-54. doi: 10.1002/pro.642. Epub 2011 May, 12. PMID:21520321<ref>PMID:21520321</ref>

-	~~===Crystal structure~~ of ~~ligand-free R7-2 streptavidin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21520321}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rdx]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_avidinii Streptomyces avidinii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA].~~	+

	==See Also==		==See Also==
	*[[Avidin\|Avidin]]		*[[Avidin\|Avidin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021520321<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
-	[[Category: Almo, S C.]]	+	[[Category: Almo, S C]]
-	[[Category: Czecster, C M.]]	+	[[Category: Czecster, C M]]
-	[[Category: Guan, R.]]	+	[[Category: Guan, R]]
-	[[Category: Levy, M.]]	+	[[Category: Levy, M]]
-	[[Category: Magalhaes, M.]]	+	[[Category: Magalhaes, M]]
	[[Category: Malashkevich, V N]]		[[Category: Malashkevich, V N]]
	[[Category: Biotin binding protein]]		[[Category: Biotin binding protein]]

OCA at 18:55, 20 October 2012

2012-10-20T18:55:52Z

←Older revision		Revision as of 18:55, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3rdx]] is a 2 chain structure ~~of [[Avidin]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA].	+	[[3rdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA].

	==See Also==		==See Also==

OCA at 05:43, 27 July 2012

2012-07-27T05:43:53Z

←Older revision		Revision as of 05:43, 27 July 2012
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-	[[Image:3rdx.~~jpg~~\|left\|200px]]	+	[[Image:3rdx.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3rdx\| PDB=3rdx \| SCENE= }}		{{STRUCTURE_3rdx\| PDB=3rdx \| SCENE= }}

	===Crystal structure of ligand-free R7-2 streptavidin===		===Crystal structure of ligand-free R7-2 streptavidin===

-
-	<!--
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	{{ABSTRACT_PUBMED_21520321}}		{{ABSTRACT_PUBMED_21520321}}

	==About this Structure==		==About this Structure==
-	[[3rdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA].	+	[[3rdx]] is a 2 chain structure of [[Avidin]] with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA].
		+
		+	==See Also==
		+	*[[Avidin\|Avidin]]

	==Reference==		==Reference==

OCA at 18:49, 6 July 2011

2011-07-06T18:49:35Z

←Older revision		Revision as of 18:49, 6 July 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3rdx.jpg\|left\|200px]]

-	The ~~entry 3rdx~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rdx", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rdx\| PDB=3rdx \| SCENE= }}

-	~~Authors: Malashkevich, V.N, Magalhaes, M., Czecster, C.M., Guan, R., Levy, M., Almo, S.C.~~	+	===Crystal structure of ligand-free R7-2 streptavidin===

-	~~Description~~: ~~Crystal structure of ligand~~-~~free R7~~-2 ~~streptavidin~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21520321}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21520321 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21520321}}
		+
		+	==About this Structure==
		+	[[3rdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDX OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021520321</ref><references group="xtra"/>
		+	[[Category: Streptomyces avidinii]]
		+	[[Category: Almo, S C.]]
		+	[[Category: Czecster, C M.]]
		+	[[Category: Guan, R.]]
		+	[[Category: Levy, M.]]
		+	[[Category: Magalhaes, M.]]
		+	[[Category: Malashkevich, V N]]
		+	[[Category: Biotin binding protein]]
		+	[[Category: Improved desthiobiotin binding]]
		+	[[Category: Opened loop destabilization]]
		+	[[Category: Streptavidin variant]]