3rem - Revision history

OCA at 12:20, 14 March 2024

2024-03-14T12:20:56Z

←Older revision		Revision as of 12:20, 14 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PCHB_PSEAE PCHB_PSEAE] Has isochorismate-pyruvate lyase activity. Probably involved in the conversion of isochorismate to salicylate and pyruvate.		[https://www.uniprot.org/uniprot/PCHB_PSEAE PCHB_PSEAE] Has isochorismate-pyruvate lyase activity. Probably involved in the conversion of isochorismate to salicylate and pyruvate.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	An isochorismate-pyruvate lyase with adventitious chorismate mutase activity from Pseudomonas aerugionsa (PchB) achieves catalysis of both pericyclic reactions in part by the stabilization of reactive conformations and in part by electrostatic transition-state stabilization. When the active site loop Lys42 is mutated to histidine, the enzyme develops a pH dependence corresponding to a loss of catalytic power upon deprotonation of the histidine. Structural data indicate that the change is not due to changes in active site architecture, but due to the difference in charge at this key site. With loss of the positive charge on the K42H side chain at high pH, the enzyme retains lyase activity at approximately 100-fold lowered catalytic efficiency but loses detectable mutase activity. We propose that both substrate organization and electrostatic transition state stabilization contribute to catalysis. However, the dominant reaction path for catalysis is dependent on reaction conditions, which influence the electrostatic properties of the enzyme active site amino acid side chains.
-
-	pH Dependence of Catalysis by Pseudomonas aeruginosa Isochorismate-Pyruvate Lyase: Implications for Transition State Stabilization and the Role of Lysine 42.,Olucha J, Ouellette AN, Luo Q, Lamb AL Biochemistry. 2011 Jul 22. PMID:21751784<ref>PMID:21751784</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3rem" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Isochorismate pyruvate lyase\|Isochorismate pyruvate lyase]]		*[[Isochorismate pyruvate lyase\|Isochorismate pyruvate lyase]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:04, 15 November 2023

2023-11-15T09:04:31Z

←Older revision		Revision as of 09:04, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='3rem' size='340' side='right'caption='[[3rem]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3rem' size='340' side='right'caption='[[3rem]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rem]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3REM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rem]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3REM FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>~~, ~~<scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~PA4230, pchB ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rem OCA], [https://pdbe.org/3rem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rem RCSB], [https://www.ebi.ac.uk/pdbsum/3rem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rem ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rem OCA], [https://pdbe.org/3rem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rem RCSB], [https://www.ebi.ac.uk/pdbsum/3rem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rem ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PCHB_PSEAE PCHB_PSEAE]] Has isochorismate-pyruvate lyase activity. Probably involved in the conversion of isochorismate to salicylate and pyruvate.	+	[https://www.uniprot.org/uniprot/PCHB_PSEAE PCHB_PSEAE] Has isochorismate-pyruvate lyase activity. Probably involved in the conversion of isochorismate to salicylate and pyruvate.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3rem" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3rem" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Isochorismate pyruvate lyase\|Isochorismate pyruvate lyase]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lamb, A L~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Luo, Q~~]]	+	[[Category: Lamb AL]]
-	[[Category: ~~Olucha, J~~]]	+	[[Category: Luo Q]]
-	[[Category: ~~Ouellette, A N~~]]	+	[[Category: Olucha J]]
-	[[Category: ~~Intertwined dimer]]~~	+	[[Category: Ouellette AN]]
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Mutase~~]]	+

OCA at 06:00, 15 June 2022

2022-06-15T06:00:16Z

←Older revision		Revision as of 06:00, 15 June 2022
Line 1:		Line 1:

	==Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate==		==Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate==
-	<StructureSection load='3rem' size='340' side='right' caption='[[3rem]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='3rem' size='340' side='right'caption='[[3rem]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rem]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3REM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rem]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3REM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4230, pchB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4230, pchB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rem OCA], [~~http~~://pdbe.org/3rem PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rem RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rem PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rem ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rem OCA], [https://pdbe.org/3rem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rem RCSB], [https://www.ebi.ac.uk/pdbsum/3rem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rem ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PCHB_PSEAE PCHB_PSEAE]] Has isochorismate-pyruvate lyase activity. Probably involved in the conversion of isochorismate to salicylate and pyruvate.	+	[[https://www.uniprot.org/uniprot/PCHB_PSEAE PCHB_PSEAE]] Has isochorismate-pyruvate lyase activity. Probably involved in the conversion of isochorismate to salicylate and pyruvate.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lamb, A L]]		[[Category: Lamb, A L]]
	[[Category: Luo, Q]]		[[Category: Luo, Q]]

OCA at 01:40, 5 August 2016

2016-08-05T01:40:33Z

←Older revision		Revision as of 01:40, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate==		==Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate==
	<StructureSection load='3rem' size='340' side='right' caption='[[3rem]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3rem' size='340' side='right' caption='[[3rem]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rem]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rem]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4230, pchB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 ~~Pseudomonas aeruginosa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4230, pchB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rem OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rem RCSB], [http://www.ebi.ac.uk/pdbsum/3rem PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rem OCA], [http://pdbe.org/3rem PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rem RCSB], [http://www.ebi.ac.uk/pdbsum/3rem PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rem ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rem" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Lamb, A L]]		[[Category: Lamb, A L]]
	[[Category: Luo, Q]]		[[Category: Luo, Q]]

OCA at 21:41, 24 December 2014

2014-12-24T21:41:09Z

←Older revision		Revision as of 21:41, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rem]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REM FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rem]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REM FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4230, pchB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4230, pchB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rem OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rem RCSB], [http://www.ebi.ac.uk/pdbsum/3rem PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rem OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rem RCSB], [http://www.ebi.ac.uk/pdbsum/3rem PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PCHB_PSEAE PCHB_PSEAE]] Has isochorismate-pyruvate lyase activity. Probably involved in the conversion of isochorismate to salicylate and pyruvate.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	pH Dependence of Catalysis by Pseudomonas aeruginosa Isochorismate-Pyruvate Lyase: Implications for Transition State Stabilization and the Role of Lysine 42.,Olucha J, Ouellette AN, Luo Q, Lamb AL Biochemistry. 2011 Jul 22. PMID:21751784<ref>PMID:21751784</ref>		pH Dependence of Catalysis by Pseudomonas aeruginosa Isochorismate-Pyruvate Lyase: Implications for Transition State Stabilization and the Role of Lysine 42.,Olucha J, Ouellette AN, Luo Q, Lamb AL Biochemistry. 2011 Jul 22. PMID:21751784<ref>PMID:21751784</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Lamb, A L.]]	+	[[Category: Lamb, A L]]
-	[[Category: Luo, Q.]]	+	[[Category: Luo, Q]]
-	[[Category: Olucha, J.]]	+	[[Category: Olucha, J]]
-	[[Category: Ouellette, A N.]]	+	[[Category: Ouellette, A N]]
	[[Category: Intertwined dimer]]		[[Category: Intertwined dimer]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Mutase]]		[[Category: Mutase]]

OCA at 04:51, 5 June 2014

2014-06-05T04:51:40Z

←Older revision		Revision as of 04:51, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3rem.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate==
		+	<StructureSection load='3rem' size='340' side='right' caption='[[3rem]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rem]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3REM FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA4230, pchB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rem OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rem RCSB], [http://www.ebi.ac.uk/pdbsum/3rem PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	An isochorismate-pyruvate lyase with adventitious chorismate mutase activity from Pseudomonas aerugionsa (PchB) achieves catalysis of both pericyclic reactions in part by the stabilization of reactive conformations and in part by electrostatic transition-state stabilization. When the active site loop Lys42 is mutated to histidine, the enzyme develops a pH dependence corresponding to a loss of catalytic power upon deprotonation of the histidine. Structural data indicate that the change is not due to changes in active site architecture, but due to the difference in charge at this key site. With loss of the positive charge on the K42H side chain at high pH, the enzyme retains lyase activity at approximately 100-fold lowered catalytic efficiency but loses detectable mutase activity. We propose that both substrate organization and electrostatic transition state stabilization contribute to catalysis. However, the dominant reaction path for catalysis is dependent on reaction conditions, which influence the electrostatic properties of the enzyme active site amino acid side chains.

-	<!--	+	pH Dependence of Catalysis by Pseudomonas aeruginosa Isochorismate-Pyruvate Lyase: Implications for Transition State Stabilization and the Role of Lysine 42.,Olucha J, Ouellette AN, Luo Q, Lamb AL Biochemistry. 2011 Jul 22. PMID:21751784<ref>PMID:21751784</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3rem", creates the "Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3rem\| PDB=3rem \| SCENE= }}~~	+

-	~~===Structure~~ of the ~~Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21751784}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21751784 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21751784}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rem]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021751784<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Lamb, A L.]]		[[Category: Lamb, A L.]]

OCA at 05:54, 31 August 2011

2011-08-31T05:54:04Z

←Older revision		Revision as of 05:54, 31 August 2011
Line 1:		Line 1:
-	[[Image:3rem.~~jpg~~\|left\|200px]]	+	[[Image:3rem.png\|left\|200px]]

	<!--		<!--

OCA at 06:04, 27 July 2011

2011-07-27T06:04:35Z

←Older revision		Revision as of 06:04, 27 July 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rem.jpg\|left\|200px]]

-	The ~~entry 3rem~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rem", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rem\| PDB=3rem \| SCENE= }}

-	~~Authors: Olucha, J., Ouellette, A.N., Luo, Q., Lamb, A.L.~~	+	===Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate===

-	~~Description~~: ~~Structure of~~ the ~~Isochorismate~~-~~Pyruvate Lyase~~ from Pseudomonas ~~aerugionsa with Bound Salicylate and Pyruvate~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21751784}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21751784 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21751784}}
		+
		+	==About this Structure==
		+	[[3rem]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3REM OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021751784</ref><references group="xtra"/>
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Lamb, A L.]]
		+	[[Category: Luo, Q.]]
		+	[[Category: Olucha, J.]]
		+	[[Category: Ouellette, A N.]]
		+	[[Category: Intertwined dimer]]
		+	[[Category: Lyase]]
		+	[[Category: Mutase]]

OCA at 06:54, 27 April 2011

2011-04-27T06:54:55Z

←Older revision		Revision as of 06:54, 27 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rem is ON HOLD	+	The entry 3rem is ON HOLD until Paper Publication

	Authors: Olucha, J., Ouellette, A.N., Luo, Q., Lamb, A.L.		Authors: Olucha, J., Ouellette, A.N., Luo, Q., Lamb, A.L.

	Description: Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate		Description: Structure of the Isochorismate-Pyruvate Lyase from Pseudomonas aerugionsa with Bound Salicylate and Pyruvate

OCA: Protected "3rem" [edit=sysop:move=sysop]

2011-04-13T04:59:58Z

Protected "3rem" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:59, 13 April 2011