3rhb - Revision history

OCA at 12:23, 14 March 2024

2024-03-14T12:23:54Z

←Older revision		Revision as of 12:23, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3rhb' size='340' side='right'caption='[[3rhb]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='3rhb' size='340' side='right'caption='[[3rhb]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rhb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RHB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rhb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RHB FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3fz9\|3fz9]], [[3rhc\|3rhc]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">GRXC5, At4g28730, F16A16.160 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhb OCA], [https://pdbe.org/3rhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rhb RCSB], [https://www.ebi.ac.uk/pdbsum/3rhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhb OCA], [https://pdbe.org/3rhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rhb RCSB], [https://www.ebi.ac.uk/pdbsum/3rhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GRXC5_ARATH GRXC5_ARATH]] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. Can assemble a [2Fe-2S] cluster, but cannot transfer it to an apoferredoxin.<ref>PMID:21632542</ref>	+	[https://www.uniprot.org/uniprot/GRXC5_ARATH GRXC5_ARATH] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. Can assemble a [2Fe-2S] cluster, but cannot transfer it to an apoferredoxin.<ref>PMID:21632542</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Unlike thioredoxins, glutaredoxins are involved in iron-sulfur cluster assembly and in reduction of specific disulfides i.e. protein-glutathione adducts, and thus they are also important redox regulators of chloroplast metabolism. Using GFP fusion, AtGrxC5 isoform, present exclusively in Brassicaceae, is shown to be localized in chloroplasts. A comparison of the biochemical, structural and spectroscopic properties of Arabidopsis GrxC5 (WCSYC active site) with poplar GrxS12 (WCSYS active site), a chloroplastic paralog, indicates that, contrary to the solely apomonomeric GrxS12 isoform, AtGrxC5 exists as two forms when expressed in Escherichia coli. The monomeric apoprotein possesses deglutathionylation activity mediating the recycling of plastidial methionine sulfoxide reductase B1 and peroxiredoxin IIE, whereas the dimeric holoprotein incorporates a [2Fe-2S] cluster. Site-directed mutagenesis experiments and resolution of the X-ray crystal structure of AtGrxC5 in its holoform revealed that, although not involved in its ligation, the presence of the second active site cysteine (Cys32) is required for cluster formation. In addition, thiol titrations, fluorescence measurements and mass spectrometry analyses show that, despite the presence of a dithiol active site, AtGrxC5 does not form any inter- or intramolecular disulfide bond and that its activity exclusively relies on a monothiol mechanism.	+
-		+
-	Arabidopsis chloroplastic glutaredoxin C5 as a model to explore the molecular determinants for iron-sulfur cluster binding into glutaredoxins.,Couturier J, Stroher E, Albetel AN, Roret T, Muthuramalingam M, Tarrago L, Seidel T, Tsan P, Jacquot JP, Johnson MK, Dietz KJ, Didierjean C, Rouhier N J Biol Chem. 2011 Jun 1. PMID:21632542<ref>PMID:21632542</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3rhb" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Couturier, J]]	+	[[Category: Couturier J]]
-	[[Category: Didierjean, C]]	+	[[Category: Didierjean C]]
-	[[Category: Jacquot~~, J P~~]]	+	[[Category: Jacquot JP]]
-	[[Category: Roret, T]]	+	[[Category: Roret T]]
-	[[Category: Rouhier, N]]	+	[[Category: Rouhier N]]
-	[[Category: Tsan, P]]	+	[[Category: Tsan P]]
-	~~[[Category: Glutaredoxin]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Thiol-disulfide oxidoreductase]]~~	+
-	~~[[Category: Thioredoxin fold~~]]	+

OCA at 06:03, 15 June 2022

2022-06-15T06:03:23Z

←Older revision		Revision as of 06:03, 15 June 2022
Line 1:		Line 1:

	==Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana==		==Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana==
-	<StructureSection load='3rhb' size='340' side='right' caption='[[3rhb]], [[Resolution\|resolution]] 1.20Å' scene=''>	+	<StructureSection load='3rhb' size='340' side='right'caption='[[3rhb]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rhb]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RHB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rhb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RHB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fz9\|3fz9]], [[3rhc\|3rhc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fz9\|3fz9]], [[3rhc\|3rhc]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GRXC5, At4g28730, F16A16.160 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GRXC5, At4g28730, F16A16.160 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhb OCA], [~~http~~://pdbe.org/3rhb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rhb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rhb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhb OCA], [https://pdbe.org/3rhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rhb RCSB], [https://www.ebi.ac.uk/pdbsum/3rhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GRXC5_ARATH GRXC5_ARATH]] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. Can assemble a [2Fe-2S] cluster, but cannot transfer it to an apoferredoxin.<ref>PMID:21632542</ref>	+	[[https://www.uniprot.org/uniprot/GRXC5_ARATH GRXC5_ARATH]] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. Can assemble a [2Fe-2S] cluster, but cannot transfer it to an apoferredoxin.<ref>PMID:21632542</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Arath]]		[[Category: Arath]]
		+	[[Category: Large Structures]]
	[[Category: Couturier, J]]		[[Category: Couturier, J]]
	[[Category: Didierjean, C]]		[[Category: Didierjean, C]]

OCA at 13:56, 11 August 2016

2016-08-11T13:56:23Z

←Older revision		Revision as of 13:56, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana==		==Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana==
	<StructureSection load='3rhb' size='340' side='right' caption='[[3rhb]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='3rhb' size='340' side='right' caption='[[3rhb]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RHB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RHB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fz9\|3fz9]], [[3rhc\|3rhc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fz9\|3fz9]], [[3rhc\|3rhc]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GRXC5, At4g28730, F16A16.160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GRXC5, At4g28730, F16A16.160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rhb RCSB], [http://www.ebi.ac.uk/pdbsum/3rhb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhb OCA], [http://pdbe.org/3rhb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rhb RCSB], [http://www.ebi.ac.uk/pdbsum/3rhb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhb ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GRXC5_ARATH GRXC5_ARATH]] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. Can assemble a [2Fe-2S] cluster, but cannot transfer it to an apoferredoxin.<ref>PMID:21632542</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rhb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabidopsis thaliana~~]]	+	[[Category: Arath]]
	[[Category: Couturier, J]]		[[Category: Couturier, J]]
	[[Category: Didierjean, C]]		[[Category: Didierjean, C]]

OCA at 10:58, 4 January 2015

2015-01-04T10:58:11Z

←Older revision		Revision as of 10:58, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RHB FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RHB FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fz9\|3fz9]], [[3rhc\|3rhc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fz9\|3fz9]], [[3rhc\|3rhc]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GRXC5, At4g28730, F16A16.160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GRXC5, At4g28730, F16A16.160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rhb RCSB], [http://www.ebi.ac.uk/pdbsum/3rhb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rhb RCSB], [http://www.ebi.ac.uk/pdbsum/3rhb PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Arabidopsis chloroplastic glutaredoxin C5 as a model to explore the molecular determinants for iron-sulfur cluster binding into glutaredoxins.,Couturier J, Stroher E, Albetel AN, Roret T, Muthuramalingam M, Tarrago L, Seidel T, Tsan P, Jacquot JP, Johnson MK, Dietz KJ, Didierjean C, Rouhier N J Biol Chem. 2011 Jun 1. PMID:21632542<ref>PMID:21632542</ref>		Arabidopsis chloroplastic glutaredoxin C5 as a model to explore the molecular determinants for iron-sulfur cluster binding into glutaredoxins.,Couturier J, Stroher E, Albetel AN, Roret T, Muthuramalingam M, Tarrago L, Seidel T, Tsan P, Jacquot JP, Johnson MK, Dietz KJ, Didierjean C, Rouhier N J Biol Chem. 2011 Jun 1. PMID:21632542<ref>PMID:21632542</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
-	[[Category: Couturier, J.]]	+	[[Category: Couturier, J]]
-	[[Category: Didierjean, C.]]	+	[[Category: Didierjean, C]]
-	[[Category: Jacquot, J P.]]	+	[[Category: Jacquot, J P]]
-	[[Category: Roret, T.]]	+	[[Category: Roret, T]]
-	[[Category: Rouhier, N.]]	+	[[Category: Rouhier, N]]
-	[[Category: Tsan, P.]]	+	[[Category: Tsan, P]]
	[[Category: Glutaredoxin]]		[[Category: Glutaredoxin]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Thiol-disulfide oxidoreductase]]		[[Category: Thiol-disulfide oxidoreductase]]
	[[Category: Thioredoxin fold]]		[[Category: Thioredoxin fold]]

OCA at 04:50, 5 June 2014

2014-06-05T04:50:01Z

←Older revision		Revision as of 04:50, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3rhb.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana==
		+	<StructureSection load='3rhb' size='340' side='right' caption='[[3rhb]], [[Resolution\|resolution]] 1.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RHB FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fz9\|3fz9]], [[3rhc\|3rhc]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GRXC5, At4g28730, F16A16.160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rhb RCSB], [http://www.ebi.ac.uk/pdbsum/3rhb PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Unlike thioredoxins, glutaredoxins are involved in iron-sulfur cluster assembly and in reduction of specific disulfides i.e. protein-glutathione adducts, and thus they are also important redox regulators of chloroplast metabolism. Using GFP fusion, AtGrxC5 isoform, present exclusively in Brassicaceae, is shown to be localized in chloroplasts. A comparison of the biochemical, structural and spectroscopic properties of Arabidopsis GrxC5 (WCSYC active site) with poplar GrxS12 (WCSYS active site), a chloroplastic paralog, indicates that, contrary to the solely apomonomeric GrxS12 isoform, AtGrxC5 exists as two forms when expressed in Escherichia coli. The monomeric apoprotein possesses deglutathionylation activity mediating the recycling of plastidial methionine sulfoxide reductase B1 and peroxiredoxin IIE, whereas the dimeric holoprotein incorporates a [2Fe-2S] cluster. Site-directed mutagenesis experiments and resolution of the X-ray crystal structure of AtGrxC5 in its holoform revealed that, although not involved in its ligation, the presence of the second active site cysteine (Cys32) is required for cluster formation. In addition, thiol titrations, fluorescence measurements and mass spectrometry analyses show that, despite the presence of a dithiol active site, AtGrxC5 does not form any inter- or intramolecular disulfide bond and that its activity exclusively relies on a monothiol mechanism.

-	~~<!-~~-	+	Arabidopsis chloroplastic glutaredoxin C5 as a model to explore the molecular determinants for iron-sulfur cluster binding into glutaredoxins.,Couturier J, Stroher E, Albetel AN, Roret T, Muthuramalingam M, Tarrago L, Seidel T, Tsan P, Jacquot JP, Johnson MK, Dietz KJ, Didierjean C, Rouhier N J Biol Chem. 2011 Jun 1. PMID:21632542<ref>PMID:21632542</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3rhb"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3rhb\| PDB=3rhb \| SCENE= }}~~	+

-	~~===Crystal structure~~ of the ~~apo form of glutaredoxin C5 from Arabidopsis thaliana===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21632542}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21632542 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21632542}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rhb]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021632542<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Couturier, J.]]		[[Category: Couturier, J.]]

OCA at 06:42, 17 August 2011

2011-08-17T06:42:22Z

←Older revision		Revision as of 06:42, 17 August 2011
Line 1:		Line 1:
-	[[Image:3rhb.~~jpg~~\|left\|200px]]	+	[[Image:3rhb.png\|left\|200px]]

	<!--		<!--

OCA at 05:19, 15 June 2011

2011-06-15T05:19:00Z

←Older revision		Revision as of 05:19, 15 June 2011
Line 11:		Line 11:
	===Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana===		===Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21632542}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21632542 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21632542}}

	==About this Structure==		==About this Structure==
	[[3rhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA].		[[3rhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021632542</ref><references group="xtra"/>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Couturier, J.]]		[[Category: Couturier, J.]]
Line 21:		Line 30:
	[[Category: Rouhier, N.]]		[[Category: Rouhier, N.]]
	[[Category: Tsan, P.]]		[[Category: Tsan, P.]]
		+	[[Category: Glutaredoxin]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Thiol-disulfide oxidoreductase]]
		+	[[Category: Thioredoxin fold]]

OCA at 05:59, 1 June 2011

2011-06-01T05:59:55Z

←Older revision		Revision as of 05:59, 1 June 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rhb.jpg\|left\|200px]]

-	The ~~entry 3rhb~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rhb", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rhb\| PDB=3rhb \| SCENE= }}

-	~~Authors: Roret, T., Couturier, J., Tsan, P., Jacquot, J.P., Rouhier, N., Didierjean, C.~~	+	===Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana===

-	~~Description: Crystal~~ structure ~~of the apo form of glutaredoxin C5~~ from Arabidopsis thaliana	+
		+	==About this Structure==
		+	[[3rhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHB OCA].
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Couturier, J.]]
		+	[[Category: Didierjean, C.]]
		+	[[Category: Jacquot, J P.]]
		+	[[Category: Roret, T.]]
		+	[[Category: Rouhier, N.]]
		+	[[Category: Tsan, P.]]

OCA at 06:55, 27 April 2011

2011-04-27T06:55:54Z

←Older revision		Revision as of 06:55, 27 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rhb is ON HOLD	+	The entry 3rhb is ON HOLD until Paper Publication

	Authors: Roret, T., Couturier, J., Tsan, P., Jacquot, J.P., Rouhier, N., Didierjean, C.		Authors: Roret, T., Couturier, J., Tsan, P., Jacquot, J.P., Rouhier, N., Didierjean, C.

	Description: Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana		Description: Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana

OCA: Protected "3rhb" [edit=sysop:move=sysop]

2011-04-21T10:22:59Z

Protected "3rhb" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:22, 21 April 2011