3rim - Revision history

OCA at 12:25, 14 March 2024

2024-03-14T12:25:15Z

←Older revision		Revision as of 12:25, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3rim' size='340' side='right'caption='[[3rim]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='3rim' size='340' side='right'caption='[[3rim]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rim]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rim]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIM FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.49Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~tkt, Rv1449c, MT1496, MTCY493.05 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83332 MYCTU])</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rim OCA], [https://pdbe.org/3rim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rim RCSB], [https://www.ebi.ac.uk/pdbsum/3rim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rim ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rim OCA], [https://pdbe.org/3rim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rim RCSB], [https://www.ebi.ac.uk/pdbsum/3rim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rim ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TKT_MYCTU TKT_MYCTU]] Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.[REFERENCE:3]	+	[https://www.uniprot.org/uniprot/TKT_MYCTU TKT_MYCTU] Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.[REFERENCE:3]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The transketolase (TKT) enzyme in Mycobacterium tuberculosis represents a novel drug target for tuberculosis treatment and has low homology with the orthologous human enzyme. Here, we report on the structural and kinetic characterization of the transketolase from M. tuberculosis (TBTKT), a homodimer whose monomers each comprise 700 amino acids. We show that TBTKT catalyses the oxidation of donor sugars xylulose-5-phosphate and fructose-6-phosphate as well as the reduction of the acceptor sugar ribose-5-phosphate. An invariant residue of the TKT consensus sequence required for thiamine cofactor binding is mutated in TBTKT; yet its catalytic activities are unaffected, and the 2.5 A resolution structure of full-length TBTKT provides an explanation for this. Key structural differences between the human and mycobacterial TKT enzymes that impact both substrate and cofactor recognition and binding were uncovered. These changes explain the kinetic differences between TBTKT and its human counterpart, and their differential inhibition by small molecules. The availability of a detailed structural model of TBTKT will enable differences between human and M. tuberculosis TKT structures to be exploited to design selective inhibitors with potential antitubercular activity.	+
-		+
-	Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme.,Fullam E, Pojer F, Bergfors T, Jones TA, Cole ST Open Biol. 2012 Jan;2(1):110026. doi: 10.1098/rsob.110026. PMID:22645655<ref>PMID:22645655</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3rim" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Transketolase 3D structures\|Transketolase 3D structures]]		*[[Transketolase 3D structures\|Transketolase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu]]~~	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	~~[[Category: Transketolase~~]]	+	[[Category: Cole ST]]
-	[[Category: Cole~~, S T~~]]	+	[[Category: Fullam E]]
-	[[Category: Fullam, E]]	+	[[Category: Jones TA]]
-	[[Category: Jones~~, T A~~]]	+	[[Category: Pojer F]]
-	[[Category: Pojer, F]]	+
-	~~[[Category: Mycobacterium]]~~	+
-	~~[[Category: Tpp]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:11, 22 June 2022

2022-06-22T10:11:59Z

←Older revision		Revision as of 10:11, 22 June 2022
Line 1:		Line 1:

	==Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)==		==Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)==
-	<StructureSection load='3rim' size='340' side='right' caption='[[3rim]], [[Resolution\|resolution]] 2.49Å' scene=''>	+	<StructureSection load='3rim' size='340' side='right'caption='[[3rim]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rim]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RIM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rim]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tkt, Rv1449c, MT1496, MTCY493.05 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tkt, Rv1449c, MT1496, MTCY493.05 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rim OCA], [~~http~~://pdbe.org/3rim PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rim RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rim PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rim ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rim OCA], [https://pdbe.org/3rim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rim RCSB], [https://www.ebi.ac.uk/pdbsum/3rim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rim ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TKT_MYCTU TKT_MYCTU]] Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.[REFERENCE:3]	+	[[https://www.uniprot.org/uniprot/TKT_MYCTU TKT_MYCTU]] Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.[REFERENCE:3]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Transketolase\|Transketolase]]	+	*[[Transketolase 3D structures\|Transketolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Myctu]]		[[Category: Myctu]]
	[[Category: Transketolase]]		[[Category: Transketolase]]

OCA at 13:43, 5 August 2016

2016-08-05T13:43:20Z

←Older revision		Revision as of 13:43, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)==		==Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)==
	<StructureSection load='3rim' size='340' side='right' caption='[[3rim]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='3rim' size='340' side='right' caption='[[3rim]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rim]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rim]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tkt, Rv1449c, MT1496, MTCY493.05 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 ~~Mycobacterium tuberculosis H37Rv~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tkt, Rv1449c, MT1496, MTCY493.05 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rim OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rim RCSB], [http://www.ebi.ac.uk/pdbsum/3rim PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rim OCA], [http://pdbe.org/3rim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rim RCSB], [http://www.ebi.ac.uk/pdbsum/3rim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rim ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rim" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mycobacterium tuberculosis h37rv~~]]	+	[[Category: Myctu]]
	[[Category: Transketolase]]		[[Category: Transketolase]]
	[[Category: Cole, S T]]		[[Category: Cole, S T]]

OCA at 19:17, 25 December 2014

2014-12-25T19:17:15Z

←Older revision		Revision as of 19:17, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rim OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rim RCSB], [http://www.ebi.ac.uk/pdbsum/3rim PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rim OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rim RCSB], [http://www.ebi.ac.uk/pdbsum/3rim PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TKT_MYCTU TKT_MYCTU]] Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.[REFERENCE:3]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:42, 9 December 2014

2014-12-09T11:42:08Z

←Older revision		Revision as of 11:42, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3rim.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)==
		+	<StructureSection load='3rim' size='340' side='right' caption='[[3rim]], [[Resolution\|resolution]] 2.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rim]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tkt, Rv1449c, MT1496, MTCY493.05 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium tuberculosis H37Rv])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rim OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rim RCSB], [http://www.ebi.ac.uk/pdbsum/3rim PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The transketolase (TKT) enzyme in Mycobacterium tuberculosis represents a novel drug target for tuberculosis treatment and has low homology with the orthologous human enzyme. Here, we report on the structural and kinetic characterization of the transketolase from M. tuberculosis (TBTKT), a homodimer whose monomers each comprise 700 amino acids. We show that TBTKT catalyses the oxidation of donor sugars xylulose-5-phosphate and fructose-6-phosphate as well as the reduction of the acceptor sugar ribose-5-phosphate. An invariant residue of the TKT consensus sequence required for thiamine cofactor binding is mutated in TBTKT; yet its catalytic activities are unaffected, and the 2.5 A resolution structure of full-length TBTKT provides an explanation for this. Key structural differences between the human and mycobacterial TKT enzymes that impact both substrate and cofactor recognition and binding were uncovered. These changes explain the kinetic differences between TBTKT and its human counterpart, and their differential inhibition by small molecules. The availability of a detailed structural model of TBTKT will enable differences between human and M. tuberculosis TKT structures to be exploited to design selective inhibitors with potential antitubercular activity.

-	~~{{STRUCTURE_3rim\| PDB=3rim \| SCENE= }}~~	+	Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme.,Fullam E, Pojer F, Bergfors T, Jones TA, Cole ST Open Biol. 2012 Jan;2(1):110026. doi: 10.1098/rsob.110026. PMID:22645655<ref>PMID:22645655</ref>

-	~~===Crystal structure~~ of ~~mycobacterium tuberculosis Transketolase (Rv1449c)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22645655}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rim]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA].~~	+

	==See Also==		==See Also==
	*[[Transketolase\|Transketolase]]		*[[Transketolase\|Transketolase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Mycobacterium tuberculosis h37rv]]		[[Category: Mycobacterium tuberculosis h37rv]]
	[[Category: Transketolase]]		[[Category: Transketolase]]
-	[[Category: Cole, S T.]]	+	[[Category: Cole, S T]]
-	[[Category: Fullam, E.]]	+	[[Category: Fullam, E]]
-	[[Category: Jones, T A.]]	+	[[Category: Jones, T A]]
-	[[Category: Pojer, F.]]	+	[[Category: Pojer, F]]
	[[Category: Mycobacterium]]		[[Category: Mycobacterium]]
	[[Category: Tpp]]		[[Category: Tpp]]
	[[Category: Transferase]]		[[Category: Transferase]]
-	[[Category: Transketolase]]

OCA at 12:46, 20 February 2013

2013-02-20T12:46:37Z

←Older revision		Revision as of 12:46, 20 February 2013
Line 5:		Line 5:
	===Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)===		===Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)===

		+	{{ABSTRACT_PUBMED_22645655}}

	==About this Structure==		==About this Structure==
-	[[3rim]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis~~ Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA].	+	[[3rim]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA].

	==See Also==		==See Also==
	*[[Transketolase\|Transketolase]]		*[[Transketolase\|Transketolase]]
-	[[Category: Mycobacterium tuberculosis]]	+	[[Category: Mycobacterium tuberculosis h37rv]]
	[[Category: Transketolase]]		[[Category: Transketolase]]
	[[Category: Cole, S T.]]		[[Category: Cole, S T.]]

OCA at 20:27, 20 October 2012

2012-10-20T20:27:22Z

←Older revision		Revision as of 20:27, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3rim]] is a 4 chain structure ~~of [[Transketolase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA].	+	[[3rim]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA].

	==See Also==		==See Also==

OCA at 10:32, 27 July 2012

2012-07-27T10:32:30Z

←Older revision		Revision as of 10:32, 27 July 2012
Line 1:		Line 1:
-	[[Image:3rim.~~jpg~~\|left\|200px]]	+	[[Image:3rim.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3rim", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3rim\| PDB=3rim \| SCENE= }}		{{STRUCTURE_3rim\| PDB=3rim \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3rim]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA].	+	[[3rim]] is a 4 chain structure of [[Transketolase]] with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA].
		+
		+	==See Also==
		+	*[[Transketolase\|Transketolase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Transketolase]]		[[Category: Transketolase]]

OCA at 08:57, 29 February 2012

2012-02-29T08:57:17Z

←Older revision		Revision as of 08:57, 29 February 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rim.jpg\|left\|200px]]

-	The ~~entry 3rim~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rim", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rim\| PDB=3rim \| SCENE= }}

-	~~Authors: Pojer, F., Fullam, E., Jones, T.A., Cole, S.T.~~	+	===Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)===

-	~~Description: Crystal~~ structure ~~of mycobacterium~~ tuberculosis Transketolase ~~(Rv1449c)~~	+
		+	==About this Structure==
		+	[[3rim]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIM OCA].
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Transketolase]]
		+	[[Category: Cole, S T.]]
		+	[[Category: Fullam, E.]]
		+	[[Category: Jones, T A.]]
		+	[[Category: Pojer, F.]]
		+	[[Category: Mycobacterium]]
		+	[[Category: Tpp]]
		+	[[Category: Transferase]]
		+	[[Category: Transketolase]]

OCA at 05:51, 15 June 2011

2011-06-15T05:51:04Z

←Older revision		Revision as of 05:51, 15 June 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rim is ON HOLD	+	The entry 3rim is ON HOLD until Paper Publication

	Authors: Pojer, F., Fullam, E., Jones, T.A., Cole, S.T.		Authors: Pojer, F., Fullam, E., Jones, T.A., Cole, S.T.

	Description: Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)		Description: Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)