3rnu - Revision history

OCA at 12:31, 14 March 2024

2024-03-14T12:31:18Z

←Older revision		Revision as of 12:31, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3rnu' size='340' side='right'caption='[[3rnu]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3rnu' size='340' side='right'caption='[[3rnu]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rnu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RNU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rnu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RNU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.502Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3rlo\|3rlo]], [[3rln\|3rln]], [[3rn5\|3rn5]], [[3rn2\|3rn2]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">IFI16, IFNGIP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rnu OCA], [https://pdbe.org/3rnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rnu RCSB], [https://www.ebi.ac.uk/pdbsum/3rnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rnu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rnu OCA], [https://pdbe.org/3rnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rnu RCSB], [https://www.ebi.ac.uk/pdbsum/3rnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rnu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/IF16_HUMAN IF16_HUMAN]] Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors.<ref>PMID:12894224</ref> <ref>PMID:9642285</ref> <ref>PMID:11146555</ref> <ref>PMID:14654789</ref> <ref>PMID:20890285</ref> <ref>PMID:21575908</ref> <ref>PMID:21573174</ref> <ref>PMID:22046441</ref> <ref>PMID:22291595</ref>	+	[https://www.uniprot.org/uniprot/IF16_HUMAN IF16_HUMAN] Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors.<ref>PMID:12894224</ref> <ref>PMID:9642285</ref> <ref>PMID:11146555</ref> <ref>PMID:14654789</ref> <ref>PMID:20890285</ref> <ref>PMID:21575908</ref> <ref>PMID:21573174</ref> <ref>PMID:22046441</ref> <ref>PMID:22291595</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Recognition of DNA by the innate immune system is central to antiviral and antibacterial defenses, as well as an important contributor to autoimmune diseases involving self DNA. AIM2 (absent in melanoma 2) and IFI16 (interferon-inducible protein 16) have been identified as DNA receptors that induce inflammasome formation and interferon production, respectively. Here we present the crystal structures of their HIN domains in complex with double-stranded (ds) DNA. Non-sequence-specific DNA recognition is accomplished through electrostatic attraction between the positively charged HIN domain residues and the dsDNA sugar-phosphate backbone. An intramolecular complex of the AIM2 Pyrin and HIN domains in an autoinhibited state is liberated by DNA binding, which may facilitate the assembly of inflammasomes along the DNA staircase. These findings provide mechanistic insights into dsDNA as the activation trigger and oligomerization platform for the assembly of large innate signaling complexes such as the inflammasomes.	+
-		+
-	Structures of the HIN Domain:DNA Complexes Reveal Ligand Binding and Activation Mechanisms of the AIM2 Inflammasome and IFI16 Receptor.,Jin T, Perry A, Jiang J, Smith P, Curry JA, Unterholzner L, Jiang Z, Horvath G, Rathinam VA, Johnstone RW, Hornung V, Latz E, Bowie AG, Fitzgerald KA, Xiao TS Immunity. 2012 Apr 20;36(4):561-71. Epub 2012 Apr 5. PMID:22483801<ref>PMID:22483801</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3rnu" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jin~~, T C~~]]	+	[[Category: Jin TC]]
-	[[Category: Xiao, T]]	+	[[Category: Xiao T]]
-	~~[[Category: Cytosolic]]~~	+
-	~~[[Category: Cytosolic dna sensor]]~~	+
-	~~[[Category: Dna binding]]~~	+
-	~~[[Category: Immune response-dna complex]]~~	+
-	~~[[Category: Ob fold]]~~	+
-	~~[[Category: Transcription activator-dna complex~~]]	+

OCA at 10:18, 22 June 2022

2022-06-22T10:18:58Z

←Older revision		Revision as of 10:18, 22 June 2022
Line 1:		Line 1:

	==Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors==		==Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors==
-	<StructureSection load='3rnu' size='340' side='right' caption='[[3rnu]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3rnu' size='340' side='right'caption='[[3rnu]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rnu]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RNU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rnu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RNU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rlo\|3rlo]], [[3rln\|3rln]], [[3rn5\|3rn5]], [[3rn2\|3rn2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rlo\|3rlo]], [[3rln\|3rln]], [[3rn5\|3rn5]], [[3rn2\|3rn2]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IFI16, IFNGIP1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IFI16, IFNGIP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rnu OCA], [~~http~~://pdbe.org/3rnu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rnu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rnu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rnu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rnu OCA], [https://pdbe.org/3rnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rnu RCSB], [https://www.ebi.ac.uk/pdbsum/3rnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rnu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/IF16_HUMAN IF16_HUMAN]] Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors.<ref>PMID:12894224</ref> <ref>PMID:9642285</ref> <ref>PMID:11146555</ref> <ref>PMID:14654789</ref> <ref>PMID:20890285</ref> <ref>PMID:21575908</ref> <ref>PMID:21573174</ref> <ref>PMID:22046441</ref> <ref>PMID:22291595</ref>	+	[[https://www.uniprot.org/uniprot/IF16_HUMAN IF16_HUMAN]] Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors.<ref>PMID:12894224</ref> <ref>PMID:9642285</ref> <ref>PMID:11146555</ref> <ref>PMID:14654789</ref> <ref>PMID:20890285</ref> <ref>PMID:21575908</ref> <ref>PMID:21573174</ref> <ref>PMID:22046441</ref> <ref>PMID:22291595</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Jin, T C]]		[[Category: Jin, T C]]
	[[Category: Xiao, T]]		[[Category: Xiao, T]]

OCA at 14:41, 11 August 2016

2016-08-11T14:41:31Z

←Older revision		Revision as of 14:41, 11 August 2016
Line 1:		Line 1:
		+
	==Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors==		==Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors==
	<StructureSection load='3rnu' size='340' side='right' caption='[[3rnu]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3rnu' size='340' side='right' caption='[[3rnu]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rlo\|3rlo]], [[3rln\|3rln]], [[3rn5\|3rn5]], [[3rn2\|3rn2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rlo\|3rlo]], [[3rln\|3rln]], [[3rn5\|3rn5]], [[3rn2\|3rn2]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IFI16, IFNGIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IFI16, IFNGIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rnu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rnu RCSB], [http://www.ebi.ac.uk/pdbsum/3rnu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rnu OCA], [http://pdbe.org/3rnu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rnu RCSB], [http://www.ebi.ac.uk/pdbsum/3rnu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rnu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rnu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:57, 28 January 2015

2015-01-28T10:57:10Z

←Older revision		Revision as of 10:57, 28 January 2015
Line 2:		Line 2:
	<StructureSection load='3rnu' size='340' side='right' caption='[[3rnu]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3rnu' size='340' side='right' caption='[[3rnu]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rnu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RNU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rnu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RNU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rlo\|3rlo]], [[3rln\|3rln]], [[3rn5\|3rn5]], [[3rn2\|3rn2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rlo\|3rlo]], [[3rln\|3rln]], [[3rn5\|3rn5]], [[3rn2\|3rn2]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IFI16, IFNGIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IFI16, IFNGIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rnu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rnu RCSB], [http://www.ebi.ac.uk/pdbsum/3rnu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rnu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rnu RCSB], [http://www.ebi.ac.uk/pdbsum/3rnu PDBsum]</span></td></tr>
	</table>		</table>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Jin, T C]]		[[Category: Jin, T C]]
	[[Category: Xiao, T]]		[[Category: Xiao, T]]

OCA at 11:27, 25 December 2014

2014-12-25T11:27:01Z

←Older revision		Revision as of 11:27, 25 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3rnu.~~png~~\|~~left~~\|~~200px~~]]	+	==Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors==
		+	<StructureSection load='3rnu' size='340' side='right' caption='[[3rnu]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rnu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RNU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rlo\|3rlo]], [[3rln\|3rln]], [[3rn5\|3rn5]], [[3rn2\|3rn2]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IFI16, IFNGIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rnu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rnu RCSB], [http://www.ebi.ac.uk/pdbsum/3rnu PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IF16_HUMAN IF16_HUMAN]] Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors.<ref>PMID:12894224</ref> <ref>PMID:9642285</ref> <ref>PMID:11146555</ref> <ref>PMID:14654789</ref> <ref>PMID:20890285</ref> <ref>PMID:21575908</ref> <ref>PMID:21573174</ref> <ref>PMID:22046441</ref> <ref>PMID:22291595</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Recognition of DNA by the innate immune system is central to antiviral and antibacterial defenses, as well as an important contributor to autoimmune diseases involving self DNA. AIM2 (absent in melanoma 2) and IFI16 (interferon-inducible protein 16) have been identified as DNA receptors that induce inflammasome formation and interferon production, respectively. Here we present the crystal structures of their HIN domains in complex with double-stranded (ds) DNA. Non-sequence-specific DNA recognition is accomplished through electrostatic attraction between the positively charged HIN domain residues and the dsDNA sugar-phosphate backbone. An intramolecular complex of the AIM2 Pyrin and HIN domains in an autoinhibited state is liberated by DNA binding, which may facilitate the assembly of inflammasomes along the DNA staircase. These findings provide mechanistic insights into dsDNA as the activation trigger and oligomerization platform for the assembly of large innate signaling complexes such as the inflammasomes.

-	~~<!--~~	+	Structures of the HIN Domain:DNA Complexes Reveal Ligand Binding and Activation Mechanisms of the AIM2 Inflammasome and IFI16 Receptor.,Jin T, Perry A, Jiang J, Smith P, Curry JA, Unterholzner L, Jiang Z, Horvath G, Rathinam VA, Johnstone RW, Hornung V, Latz E, Bowie AG, Fitzgerald KA, Xiao TS Immunity. 2012 Apr 20;36(4):561-71. Epub 2012 Apr 5. PMID:22483801<ref>PMID:22483801</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3rnu", creates~~ the ~~"Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3rnu\| PDB=3rnu \| SCENE= }}~~	+

-	~~===Structural Basis~~ of ~~Cytosolic DNA Sensing by Innate Immune Receptors===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22483801}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22483801 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22483801}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rnu]] is a 6 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022483801<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Jin, T C.]]	+	[[Category: Jin, T C]]
-	[[Category: Xiao, T.]]	+	[[Category: Xiao, T]]
	[[Category: Cytosolic]]		[[Category: Cytosolic]]
	[[Category: Cytosolic dna sensor]]		[[Category: Cytosolic dna sensor]]

OCA at 08:21, 9 May 2012

2012-05-09T08:21:09Z

←Older revision		Revision as of 08:21, 9 May 2012
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-	[[Image:3rnu.~~jpg~~\|left\|200px]]	+	[[Image:3rnu.png\|left\|200px]]

	<!--		<!--
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	===Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors===		===Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22483801}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22483801 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22483801}}

	==About this Structure==		==About this Structure==
	[[3rnu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA].		[[3rnu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022483801</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Jin, T C.]]		[[Category: Jin, T C.]]

OCA at 09:21, 25 April 2012

2012-04-25T09:21:44Z

←Older revision		Revision as of 09:21, 25 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rnu.jpg\|left\|200px]]

-	The ~~entry 3rnu~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rnu", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rnu\| PDB=3rnu \| SCENE= }}

-	~~Authors: Jin, T.C., Xiao, T.~~	+	===Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors===

-	~~Description~~: ~~Structural Basis of~~ Cytosolic ~~DNA Sensing by Innate~~ Immune ~~Receptors~~	+
		+	==About this Structure==
		+	[[3rnu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RNU OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Jin, T C.]]
		+	[[Category: Xiao, T.]]
		+	[[Category: Cytosolic]]
		+	[[Category: Cytosolic dna sensor]]
		+	[[Category: Dna binding]]
		+	[[Category: Immune response-dna complex]]
		+	[[Category: Ob fold]]
		+	[[Category: Transcription activator-dna complex]]

OCA at 05:14, 18 May 2011

2011-05-18T05:14:05Z

←Older revision		Revision as of 05:14, 18 May 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rnu is ON HOLD	+	The entry 3rnu is ON HOLD until Paper Publication

	Authors: Jin, T.C., Xiao, T.		Authors: Jin, T.C., Xiao, T.

	Description: Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors		Description: Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors

OCA: Protected "3rnu" [edit=sysop:move=sysop]

2011-05-04T06:33:53Z

Protected "3rnu" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:33, 4 May 2011

OCA: New page: '''Unreleased structure''' The entry 3rnu is ON HOLD Authors: Jin, T.C., Xiao, T. Description: Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors

2011-05-04T06:33:52Z

New page: '''Unreleased structure''' The entry 3rnu is ON HOLD Authors: Jin, T.C., Xiao, T. Description: Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors

New page

'''Unreleased structure'''

The entry 3rnu is ON HOLD

Authors: Jin, T.C., Xiao, T.

Description: Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors