3rrr - Revision history

OCA at 12:35, 14 March 2024

2024-03-14T12:35:15Z

←Older revision		Revision as of 12:35, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rrr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Respiratory_syncytial_virus Respiratory syncytial virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RRR FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rrr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Respiratory_syncytial_virus Respiratory syncytial virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RRR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAG:N~~-~~ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.821Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3rrt\|3rrt]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rrr OCA], [https://pdbe.org/3rrr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rrr RCSB], [https://www.ebi.ac.uk/pdbsum/3rrr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rrr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rrr OCA], [https://pdbe.org/3rrr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rrr RCSB], [https://www.ebi.ac.uk/pdbsum/3rrr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rrr ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q84850_HRSV Q84850_HRSV]
-	Respiratory syncytial virus (RSV) invades host cells via a type I fusion (F) glycoprotein that undergoes dramatic structural rearrangements during the fusion process. Neutralizing monoclonal antibodies such as 101F, palivizumab, and motavizumab, target two major antigenic sites on the RSV F glycoprotein. Structures of these sites as peptide complexes with motavizumab and 101F have been previously determined, but a structure of the trimeric RSV F glycoprotein ectodomain has remained elusive. To address this issue, we undertook structural and biophysical studies on stable ectodomain constructs. Here we present the 2.8 A crystal structure of the trimeric RSV F ectodomain in its post-fusion conformation. The structure revealed that the 101F and motavizumab epitopes are present in the post-fusion state, and that their conformations are similar to those observed in the antibody-bound peptide structures. Both antibodies bound the post-fusion F glycoprotein with high affinity in surface plasmon resonance experiments. Modeling of the antibodies bound to the F glycoprotein predicts that the 101F epitope is larger than the linear peptide and restricted to a single protomer in the trimer, whereas motavizumab likely contacts residues on two protomers, indicating a quaternary epitope. Mechanistically, these results suggest that 101F and motavizumab can bind to multiple conformations of the fusion glycoprotein, and neutralize late in the fusion process. The structural preservation of neutralizing epitopes in the post-fusion state suggests that this conformation can elicit neutralizing antibodies and serve as a useful vaccine antigen.	+
-		+
-	Structure of the Respiratory Syncytial Virus Fusion Glycoprotein in the Post-fusion Conformation Reveals Preservation of Neutralizing Epitopes.,McLellan JS, Yang Y, Graham BS, Kwong PD J Virol. 2011 May 25. PMID:~~21613394<ref>PMID:21613394<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3rrr" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Respiratory syncytial virus]]		[[Category: Respiratory syncytial virus]]
-	[[Category: Graham~~, B S~~]]	+	[[Category: Graham BS]]
-	[[Category: Kwong~~, P D~~]]	+	[[Category: Kwong PD]]
-	[[Category: McLellan~~, J S~~]]	+	[[Category: McLellan JS]]
-	[[Category: Yongping, Y]]	+	[[Category: Yongping Y]]
-	~~[[Category: Membrane fusion]]~~	+
-	~~[[Category: Six-helix bundle]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 10:23, 22 June 2022

2022-06-22T10:23:15Z

←Older revision		Revision as of 10:23, 22 June 2022
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	==Structure of the RSV F protein in the post-fusion conformation==		==Structure of the RSV F protein in the post-fusion conformation==
-	<StructureSection load='3rrr' size='340' side='right' caption='[[3rrr]], [[Resolution\|resolution]] 2.82Å' scene=''>	+	<StructureSection load='3rrr' size='340' side='right'caption='[[3rrr]], [[Resolution\|resolution]] 2.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rrr]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Respiratory_syncytial_virus Respiratory syncytial virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RRR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rrr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Respiratory_syncytial_virus Respiratory syncytial virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RRR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rrt\|3rrt]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rrt\|3rrt]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rrr OCA], [~~http~~://pdbe.org/3rrr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rrr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rrr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rrr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rrr OCA], [https://pdbe.org/3rrr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rrr RCSB], [https://www.ebi.ac.uk/pdbsum/3rrr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rrr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Respiratory syncytial virus]]		[[Category: Respiratory syncytial virus]]
	[[Category: Graham, B S]]		[[Category: Graham, B S]]

OCA at 20:50, 5 August 2016

2016-08-05T20:50:44Z

←Older revision		Revision as of 20:50, 5 August 2016
Line 1:		Line 1:
-	{{STRUCTURE_3rrr\| PDB=3rrr \| SCENE= }}
-	===Structure of the RSV F protein in the post-fusion conformation===
-	{{ABSTRACT_PUBMED_21613394}}

-	==~~About this~~ Structure==	+	==Structure of the RSV F protein in the post-fusion conformation==
-	[[3rrr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Respiratory_syncytial_virus Respiratory syncytial virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRR OCA].	+	<StructureSection load='3rrr' size='340' side='right' caption='[[3rrr]], [[Resolution\|resolution]] 2.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rrr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Respiratory_syncytial_virus Respiratory syncytial virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RRR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rrt\|3rrt]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rrr OCA], [http://pdbe.org/3rrr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rrr RCSB], [http://www.ebi.ac.uk/pdbsum/3rrr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rrr ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Respiratory syncytial virus (RSV) invades host cells via a type I fusion (F) glycoprotein that undergoes dramatic structural rearrangements during the fusion process. Neutralizing monoclonal antibodies such as 101F, palivizumab, and motavizumab, target two major antigenic sites on the RSV F glycoprotein. Structures of these sites as peptide complexes with motavizumab and 101F have been previously determined, but a structure of the trimeric RSV F glycoprotein ectodomain has remained elusive. To address this issue, we undertook structural and biophysical studies on stable ectodomain constructs. Here we present the 2.8 A crystal structure of the trimeric RSV F ectodomain in its post-fusion conformation. The structure revealed that the 101F and motavizumab epitopes are present in the post-fusion state, and that their conformations are similar to those observed in the antibody-bound peptide structures. Both antibodies bound the post-fusion F glycoprotein with high affinity in surface plasmon resonance experiments. Modeling of the antibodies bound to the F glycoprotein predicts that the 101F epitope is larger than the linear peptide and restricted to a single protomer in the trimer, whereas motavizumab likely contacts residues on two protomers, indicating a quaternary epitope. Mechanistically, these results suggest that 101F and motavizumab can bind to multiple conformations of the fusion glycoprotein, and neutralize late in the fusion process. The structural preservation of neutralizing epitopes in the post-fusion state suggests that this conformation can elicit neutralizing antibodies and serve as a useful vaccine antigen.

-	~~==Reference==~~	+	Structure of the Respiratory Syncytial Virus Fusion Glycoprotein in the Post-fusion Conformation Reveals Preservation of Neutralizing Epitopes.,McLellan JS, Yang Y, Graham BS, Kwong PD J Virol. 2011 May 25. PMID:21613394<ref>PMID:21613394</ref>
-	<ref ~~group="xtra"~~>PMID:~~021613394~~</ref><~~references group~~="~~xtra~~"/><references/>	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3rrr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Respiratory syncytial virus]]		[[Category: Respiratory syncytial virus]]
-	[[Category: Graham, B S.]]	+	[[Category: Graham, B S]]
-	[[Category: Kwong, P D.]]	+	[[Category: Kwong, P D]]
-	[[Category: McLellan, J S.]]	+	[[Category: McLellan, J S]]
-	[[Category: Yongping, Y.]]	+	[[Category: Yongping, Y]]
	[[Category: Membrane fusion]]		[[Category: Membrane fusion]]
	[[Category: Six-helix bundle]]		[[Category: Six-helix bundle]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 07:33, 2 April 2014

2014-04-02T07:33:11Z

←Older revision		Revision as of 07:33, 2 April 2014
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-	[[Image:3rrr.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3rrr", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3rrr\| PDB=3rrr \| SCENE= }}		{{STRUCTURE_3rrr\| PDB=3rrr \| SCENE= }}
-
	===Structure of the RSV F protein in the post-fusion conformation===		===Structure of the RSV F protein in the post-fusion conformation===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21613394}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21613394 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21613394}}		{{ABSTRACT_PUBMED_21613394}}

	==About this Structure==		==About this Structure==
-	[[3rrr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Human_respiratory_syncytial_virus Human respiratory~~ syncytial virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRR OCA].	+	[[3rrr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Respiratory_syncytial_virus Respiratory syncytial virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRR OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:021613394</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021613394</ref><references group="xtra"/><references/>
-	[[Category: ~~Human respiratory~~ syncytial virus]]	+	[[Category: Respiratory syncytial virus]]
	[[Category: Graham, B S.]]		[[Category: Graham, B S.]]
	[[Category: Kwong, P D.]]		[[Category: Kwong, P D.]]

OCA at 05:13, 10 August 2011

2011-08-10T05:13:00Z

←Older revision		Revision as of 05:13, 10 August 2011
Line 1:		Line 1:
-	[[Image:3rrr.~~jpg~~\|left\|200px]]	+	[[Image:3rrr.png\|left\|200px]]

	<!--		<!--
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	[[Category: McLellan, J S.]]		[[Category: McLellan, J S.]]
	[[Category: Yongping, Y.]]		[[Category: Yongping, Y.]]
		+	[[Category: Membrane fusion]]
		+	[[Category: Six-helix bundle]]
		+	[[Category: Viral protein]]

OCA at 05:11, 8 June 2011

2011-06-08T05:11:21Z

←Older revision		Revision as of 05:11, 8 June 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rrr.jpg\|left\|200px]]

-	The ~~entry 3rrr~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rrr", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rrr\| PDB=3rrr \| SCENE= }}

-	~~Authors: McLellan, J.S., Yongping, Y., Graham, B.S., Kwong, P.D.~~	+	===Structure of the RSV F protein in the post-fusion conformation===

-	~~Description: Structure of~~ the ~~RSV F protein in~~ the ~~post~~-~~fusion conformation~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21613394}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21613394 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21613394}}
		+
		+	==About this Structure==
		+	[[3rrr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus Human respiratory syncytial virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRR OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021613394</ref><references group="xtra"/>
		+	[[Category: Human respiratory syncytial virus]]
		+	[[Category: Graham, B S.]]
		+	[[Category: Kwong, P D.]]
		+	[[Category: McLellan, J S.]]
		+	[[Category: Yongping, Y.]]

OCA: Protected "3rrr" [edit=sysop:move=sysop]

2011-05-12T04:22:35Z

Protected "3rrr" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:22, 12 May 2011

OCA: New page: '''Unreleased structure''' The entry 3rrr is ON HOLD Authors: McLellan, J.S., Yongping, Y., Graham, B.S., Kwong, P.D. Description: Structure of the RSV F protein in the post-fusion con...

2011-05-12T04:22:34Z

New page: '''Unreleased structure''' The entry 3rrr is ON HOLD Authors: McLellan, J.S., Yongping, Y., Graham, B.S., Kwong, P.D. Description: Structure of the RSV F protein in the post-fusion con...

New page

'''Unreleased structure'''

The entry 3rrr is ON HOLD

Authors: McLellan, J.S., Yongping, Y., Graham, B.S., Kwong, P.D.

Description: Structure of the RSV F protein in the post-fusion conformation