3rur - Revision history

OCA at 12:38, 14 March 2024

2024-03-14T12:38:22Z

←Older revision		Revision as of 12:38, 14 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ISDB_STAAN ISDB_STAAN] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).		[https://www.uniprot.org/uniprot/ISDB_STAAN ISDB_STAAN] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Iron is an essential requirement for life for nearly all organisms. The human pathogen Staphylococcus aureus is able to acquire iron from the heme cofactor of hemoglobin (Hb) released from lysed erythrocytes. IsdB, the predominant Hb receptor of S. aureus, is a cell wall-anchored protein that is composed of two NEAT domains. The N-terminal NEAT domain (IsdB-N1) binds Hb, and the C-terminal NEAT domain (IsdB-N2) relays heme to IsdA for transport into the cell. Here we present the 1.45 A resolution X-ray crystal structure of the IsdB-N2-heme complex. While the structure largely conforms to the eight-strand beta-sandwich fold seen in other NEAT domains such as IsdA-N and uses a conserved Tyr residue to coordinate heme-iron, a Met residue is also involved in iron coordination, resulting in a novel Tyr-Met hexacoordinate heme-iron state. The kinetics of the transfer of heme from IsdB-N2 to IsdA-N can be modeled as a two-step process. The rate of transfer of heme between the isolated NEAT domains (82 s(-1)) was found to be similar to that measured for the full-length proteins. Replacing the iron coordinating Met with Leu did not abrogate high-affinity heme binding but did reduce the heme transfer rate constant by more than half. This unusual Met-Tyr heme coordination may also bestow properties on IsdB that help it to bind heme in different oxidation states or extract heme from hemoglobin.
-
-	Unique Heme-Iron Coordination by the Hemoglobin Receptor IsdB of Staphylococcus aureus.,Gaudin CF, Grigg JC, Arrieta AL, Murphy ME Biochemistry. 2011 Jun 21;50(24):5443-5452. Epub 2011 May 26. PMID:21574663<ref>PMID:21574663</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3rur" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:14, 6 December 2023

2023-12-06T08:14:50Z

←Older revision		Revision as of 08:14, 6 December 2023
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	<StructureSection load='3rur' size='340' side='right'caption='[[3rur]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3rur' size='340' side='right'caption='[[3rur]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rur]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Staan Staan~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RUR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rur]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_N315 Staphylococcus aureus subsp. aureus N315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RUR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">frpB, isdB, SA0976, sasJ, sirH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158879 STAAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rur OCA], [https://pdbe.org/3rur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rur RCSB], [https://www.ebi.ac.uk/pdbsum/3rur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rur ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rur OCA], [https://pdbe.org/3rur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rur RCSB], [https://www.ebi.ac.uk/pdbsum/3rur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rur ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ISDB_STAAN ISDB_STAAN]] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).	+	[https://www.uniprot.org/uniprot/ISDB_STAAN ISDB_STAAN] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staan~~]]	+	[[Category: Staphylococcus aureus subsp. aureus N315]]
-	[[Category: Arrieta~~, A L~~]]	+	[[Category: Arrieta AL]]
-	[[Category: Gaudin~~, C F.M~~]]	+	[[Category: Gaudin CFM]]
-	[[Category: Grigg~~, J C~~]]	+	[[Category: Grigg JC]]
-	[[Category: Murphy~~, M E.P]]~~	+	[[Category: Murphy MEP]]
-	~~[[Category: Cell wall]]~~	+
-	~~[[Category: Heme binding]]~~	+
-	~~[[Category: Heme protein]]~~	+
-	~~[[Category: Heme uptake]]~~	+
-	~~[[Category: Metal transport]]~~	+
-	~~[[Category: Neat domain~~]]	+

OCA at 10:26, 22 June 2022

2022-06-22T10:26:04Z

←Older revision		Revision as of 10:26, 22 June 2022
Line 1:		Line 1:

	==Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2==		==Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2==
-	<StructureSection load='3rur' size='340' side='right' caption='[[3rur]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3rur' size='340' side='right'caption='[[3rur]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rur]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staan Staan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RUR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rur]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staan Staan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RUR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">frpB, isdB, SA0976, sasJ, sirH ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158879 STAAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">frpB, isdB, SA0976, sasJ, sirH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158879 STAAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rur OCA], [~~http~~://pdbe.org/3rur PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rur RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rur PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rur ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rur OCA], [https://pdbe.org/3rur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rur RCSB], [https://www.ebi.ac.uk/pdbsum/3rur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rur ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ISDB_STAAN ISDB_STAAN]] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).	+	[[https://www.uniprot.org/uniprot/ISDB_STAAN ISDB_STAAN]] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staan]]		[[Category: Staan]]
	[[Category: Arrieta, A L]]		[[Category: Arrieta, A L]]

OCA at 22:47, 4 August 2016

2016-08-04T22:47:18Z

←Older revision		Revision as of 22:47, 4 August 2016
Line 1:		Line 1:
		+
	==Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2==		==Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2==
	<StructureSection load='3rur' size='340' side='right' caption='[[3rur]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3rur' size='340' side='right' caption='[[3rur]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RUR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staan Staan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RUR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">frpB, isdB, SA0976, sasJ, sirH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~46170 Staphylococcus aureus subsp. aureus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">frpB, isdB, SA0976, sasJ, sirH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158879 STAAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rur OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rur RCSB], [http://www.ebi.ac.uk/pdbsum/3rur PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rur OCA], [http://pdbe.org/3rur PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rur RCSB], [http://www.ebi.ac.uk/pdbsum/3rur PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rur ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ISDB_STAAN ISDB_STAAN]] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rur" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Staphylococcus aureus subsp. aureus~~]]	+	[[Category: Staan]]
	[[Category: Arrieta, A L]]		[[Category: Arrieta, A L]]
	[[Category: Gaudin, C F.M]]		[[Category: Gaudin, C F.M]]

OCA at 10:16, 19 December 2014

2014-12-19T10:16:00Z

←Older revision		Revision as of 10:16, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3rur~~\| ~~PDB~~=3rur \| ~~SCENE~~= }}	+	==Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2==
-	===Staphylococcus aureus ~~heme~~-~~bound selenomethionine~~-~~labeled IsdB~~-N2===	+	<StructureSection load='3rur' size='340' side='right' caption='[[3rur]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21574663}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RUR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">frpB, isdB, SA0976, sasJ, sirH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rur OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rur RCSB], [http://www.ebi.ac.uk/pdbsum/3rur PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Iron is an essential requirement for life for nearly all organisms. The human pathogen Staphylococcus aureus is able to acquire iron from the heme cofactor of hemoglobin (Hb) released from lysed erythrocytes. IsdB, the predominant Hb receptor of S. aureus, is a cell wall-anchored protein that is composed of two NEAT domains. The N-terminal NEAT domain (IsdB-N1) binds Hb, and the C-terminal NEAT domain (IsdB-N2) relays heme to IsdA for transport into the cell. Here we present the 1.45 A resolution X-ray crystal structure of the IsdB-N2-heme complex. While the structure largely conforms to the eight-strand beta-sandwich fold seen in other NEAT domains such as IsdA-N and uses a conserved Tyr residue to coordinate heme-iron, a Met residue is also involved in iron coordination, resulting in a novel Tyr-Met hexacoordinate heme-iron state. The kinetics of the transfer of heme from IsdB-N2 to IsdA-N can be modeled as a two-step process. The rate of transfer of heme between the isolated NEAT domains (82 s(-1)) was found to be similar to that measured for the full-length proteins. Replacing the iron coordinating Met with Leu did not abrogate high-affinity heme binding but did reduce the heme transfer rate constant by more than half. This unusual Met-Tyr heme coordination may also bestow properties on IsdB that help it to bind heme in different oxidation states or extract heme from hemoglobin.

-	~~==About this Structure==~~	+	Unique Heme-Iron Coordination by the Hemoglobin Receptor IsdB of Staphylococcus aureus.,Gaudin CF, Grigg JC, Arrieta AL, Murphy ME Biochemistry. 2011 Jun 21;50(24):5443-5452. Epub 2011 May 26. PMID:21574663<ref>PMID:21574663</ref>
-	~~[[3rur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus~~ Staphylococcus aureus ~~subsp~~. ~~aureus]~~. ~~Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3RUR OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:021574663</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
-	[[Category: Arrieta, A L.]]	+	[[Category: Arrieta, A L]]
-	[[Category: Gaudin, C F.M.]]	+	[[Category: Gaudin, C F.M]]
-	[[Category: Grigg, J C.]]	+	[[Category: Grigg, J C]]
-	[[Category: Murphy, M E.P.]]	+	[[Category: Murphy, M E.P]]
	[[Category: Cell wall]]		[[Category: Cell wall]]
	[[Category: Heme binding]]		[[Category: Heme binding]]

OCA at 07:50, 16 May 2013

2013-05-16T07:50:50Z

←Older revision		Revision as of 07:50, 16 May 2013
Line 1:		Line 1:
-	[[Image:3rur.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3rur", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3rur\| PDB=3rur \| SCENE= }}		{{STRUCTURE_3rur\| PDB=3rur \| SCENE= }}
-
	===Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2===		===Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21574663}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21574663 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21574663}}		{{ABSTRACT_PUBMED_21574663}}

Line 22:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:021574663</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021574663</ref><references group="xtra"/><references/>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
	[[Category: Arrieta, A L.]]		[[Category: Arrieta, A L.]]

OCA at 15:47, 22 June 2011

2011-06-22T15:47:38Z

←Older revision		Revision as of 15:47, 22 June 2011
Line 1:		Line 1:
-	[[Image:3rur.~~jpg~~\|left\|200px]]	+	[[Image:3rur.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2===		===Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21574663}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21574663 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21574663}}

	==About this Structure==		==About this Structure==
	[[3rur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA].		[[3rur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021574663</ref><references group="xtra"/>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
	[[Category: Arrieta, A L.]]		[[Category: Arrieta, A L.]]
Line 19:		Line 28:
	[[Category: Grigg, J C.]]		[[Category: Grigg, J C.]]
	[[Category: Murphy, M E.P.]]		[[Category: Murphy, M E.P.]]
		+	[[Category: Cell wall]]
		+	[[Category: Heme binding]]
		+	[[Category: Heme protein]]
		+	[[Category: Heme uptake]]
		+	[[Category: Metal transport]]
		+	[[Category: Neat domain]]

OCA at 05:58, 18 May 2011

2011-05-18T05:58:16Z

←Older revision		Revision as of 05:58, 18 May 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rur.jpg\|left\|200px]]

-	The ~~entry 3rur~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rur", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rur\| PDB=3rur \| SCENE= }}

-	~~Authors: Gaudin, C.F.M., Grigg, J.C., Arrieta, A.L., Murphy, M.E.P.~~	+	===Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2===

-	~~Description~~: Staphylococcus aureus ~~heme~~-~~bound selenomethionine-labeled IsdB-N2~~	+
		+	==About this Structure==
		+	[[3rur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RUR OCA].
		+	[[Category: Staphylococcus aureus subsp. aureus]]
		+	[[Category: Arrieta, A L.]]
		+	[[Category: Gaudin, C F.M.]]
		+	[[Category: Grigg, J C.]]
		+	[[Category: Murphy, M E.P.]]

OCA: Protected "3rur" [edit=sysop:move=sysop]

2011-05-12T04:25:35Z

Protected "3rur" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:25, 12 May 2011

OCA: New page: '''Unreleased structure''' The entry 3rur is ON HOLD Authors: Gaudin, C.F.M., Grigg, J.C., Arrieta, A.L., Murphy, M.E.P. Description: Staphylococcus aureus heme-bound selenomethionine-...

2011-05-12T04:25:34Z

New page: '''Unreleased structure''' The entry 3rur is ON HOLD Authors: Gaudin, C.F.M., Grigg, J.C., Arrieta, A.L., Murphy, M.E.P. Description: Staphylococcus aureus heme-bound selenomethionine-...

New page

'''Unreleased structure'''

The entry 3rur is ON HOLD

Authors: Gaudin, C.F.M., Grigg, J.C., Arrieta, A.L., Murphy, M.E.P.

Description: Staphylococcus aureus heme-bound selenomethionine-labeled IsdB-N2