3s7y - Revision history

OCA at 12:49, 14 March 2024

2024-03-14T12:49:35Z

←Older revision		Revision as of 12:49, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3s7y' size='340' side='right'caption='[[3s7y]], [[Resolution\|resolution]] 4.31Å' scene=''>		<StructureSection load='3s7y' size='340' side='right'caption='[[3s7y]], [[Resolution\|resolution]] 4.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Marmm Marmm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S7Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Maricaulis_maris_MCS10 Maricaulis maris MCS10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S7Y FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3s6g\|3s6g]], [[3s6h\|3s6h]], [[3s6k\|3s6k]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.3077Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=394221 MARMM])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s7y OCA], [https://pdbe.org/3s7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s7y RCSB], [https://www.ebi.ac.uk/pdbsum/3s7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s7y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s7y OCA], [https://pdbe.org/3s7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s7y RCSB], [https://www.ebi.ac.uk/pdbsum/3s7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s7y ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q0ASS9_MARMM Q0ASS9_MARMM]
-	Novel bifunctional N-acetylglutamate synthase/kinases (NAGS/K) that catalyze the first two steps of arginine biosynthesis and are homologous to vertebrate N-acetylglutamate synthase (NAGS), an essential cofactor-producing enzyme in the urea cycle, were identified in Maricaulis maris and several other bacteria. Arginine is an allosteric inhibitor of NAGS but not NAGK activity. The crystal structure of M. maris NAGS/K (mmNAGS/K) at 2.7 A resolution indicates that it is a tetramer, in contrast to the hexameric structure of Neisseria gonorrhoeae NAGS. The quaternary structure of crystalline NAGS/K from Xanthomonas campestris (xcNAGS/K) is similar, and cross-linking experiments indicate that both mmNAGS/K and xcNAGS are tetramers in solution. Each subunit has an amino acid kinase (AAK) domain, which is likely responsible for N-acetylglutamate kinase (NAGK) activity and has a putative arginine binding site, and an N-acetyltransferase (NAT) domain that contains the putative NAGS active site. These structures and sequence comparisons suggest that the linker residue 291 may determine whether arginine acts as an allosteric inhibitor or activator in homologous enzymes in microorganisms and vertebrates. In addition, the angle of rotation between AAK and NAT domains varies among crystal forms and subunits within the tetramer. A rotation of 26 degrees is sufficient to close the predicted AcCoA binding site, thus reducing enzymatic activity. Since mmNAGS/K has the highest degree of sequence homology to vertebrate NAGS of NAGS and NAGK enzymes whose structures have been determined, the mmNAGS/K structure was used to develop a structural model of human NAGS that is fully consistent with the functional effects of the 14 missense mutations that were identified in NAGS-deficient patients.	+
-		+
-	A Novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris.,Shi D, Li Y, Cabrera-Luque J, Jin Z, Yu X, Zhao G, Haskins N, Allewell NM, Tuchman M PLoS One. 2011;6(12):~~e28825. Epub 2011 Dec 12. PMID:22174908<ref>PMID:22174908<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3s7y" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Marmm~~]]	+	[[Category: Maricaulis maris MCS10]]
-	[[Category: Allewell~~, N M~~]]	+	[[Category: Allewell NM]]
-	[[Category: Cabrera-Luque, J]]	+	[[Category: Cabrera-Luque J]]
-	[[Category: Jin, Z]]	+	[[Category: Jin Z]]
-	[[Category: Li, Y]]	+	[[Category: Li Y]]
-	[[Category: Shi, D]]	+	[[Category: Shi D]]
-	[[Category: Tuchman, M]]	+	[[Category: Tuchman M]]
-	[[Category: Yu, X]]	+	[[Category: Yu X]]
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Synthase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:41, 22 June 2022

2022-06-22T10:41:55Z

←Older revision		Revision as of 10:41, 22 June 2022
Line 1:		Line 1:

	==Crystal structure of mmNAGS in Space Group P3121 at 4.3 A resolution==		==Crystal structure of mmNAGS in Space Group P3121 at 4.3 A resolution==
-	<StructureSection load='3s7y' size='340' side='right' caption='[[3s7y]], [[Resolution\|resolution]] 4.31Å' scene=''>	+	<StructureSection load='3s7y' size='340' side='right'caption='[[3s7y]], [[Resolution\|resolution]] 4.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s7y]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Marmm Marmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3S7Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Marmm Marmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S7Y FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6g\|3s6g]], [[3s6h\|3s6h]], [[3s6k\|3s6k]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3s6g\|3s6g]], [[3s6h\|3s6h]], [[3s6k\|3s6k]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=394221 MARMM])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=394221 MARMM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3s7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s7y OCA], [~~http~~://pdbe.org/3s7y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3s7y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3s7y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3s7y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s7y OCA], [https://pdbe.org/3s7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s7y RCSB], [https://www.ebi.ac.uk/pdbsum/3s7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s7y ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Marmm]]		[[Category: Marmm]]
	[[Category: Allewell, N M]]		[[Category: Allewell, N M]]

OCA at 08:26, 11 August 2016

2016-08-11T08:26:47Z

←Older revision		Revision as of 08:26, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of mmNAGS in Space Group P3121 at 4.3 A resolution==		==Crystal structure of mmNAGS in Space Group P3121 at 4.3 A resolution==
	<StructureSection load='3s7y' size='340' side='right' caption='[[3s7y]], [[Resolution\|resolution]] 4.31Å' scene=''>		<StructureSection load='3s7y' size='340' side='right' caption='[[3s7y]], [[Resolution\|resolution]] 4.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s7y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Maricaulis_maris Maricaulis maris~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S7Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s7y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Marmm Marmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S7Y FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6g\|3s6g]], [[3s6h\|3s6h]], [[3s6k\|3s6k]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6g\|3s6g]], [[3s6h\|3s6h]], [[3s6k\|3s6k]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~74318 Maricaulis maris~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=394221 MARMM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s7y RCSB], [http://www.ebi.ac.uk/pdbsum/3s7y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s7y OCA], [http://pdbe.org/3s7y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s7y RCSB], [http://www.ebi.ac.uk/pdbsum/3s7y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3s7y ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3s7y" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Maricaulis maris~~]]	+	[[Category: Marmm]]
	[[Category: Allewell, N M]]		[[Category: Allewell, N M]]
	[[Category: Cabrera-Luque, J]]		[[Category: Cabrera-Luque, J]]

OCA at 10:36, 4 January 2015

2015-01-04T10:36:18Z

←Older revision		Revision as of 10:36, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3s7y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maricaulis_maris Maricaulis maris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S7Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[3s7y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maricaulis_maris Maricaulis maris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S7Y FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6g\|3s6g]], [[3s6h\|3s6h]], [[3s6k\|3s6k]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6g\|3s6g]], [[3s6h\|3s6h]], [[3s6k\|3s6k]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=74318 Maricaulis maris])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=74318 Maricaulis maris])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s7y RCSB], [http://www.ebi.ac.uk/pdbsum/3s7y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s7y RCSB], [http://www.ebi.ac.uk/pdbsum/3s7y PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	A Novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris.,Shi D, Li Y, Cabrera-Luque J, Jin Z, Yu X, Zhao G, Haskins N, Allewell NM, Tuchman M PLoS One. 2011;6(12):e28825. Epub 2011 Dec 12. PMID:22174908<ref>PMID:22174908</ref>		A Novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris.,Shi D, Li Y, Cabrera-Luque J, Jin Z, Yu X, Zhao G, Haskins N, Allewell NM, Tuchman M PLoS One. 2011;6(12):e28825. Epub 2011 Dec 12. PMID:22174908<ref>PMID:22174908</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Maricaulis maris]]		[[Category: Maricaulis maris]]
-	[[Category: Allewell, N M.]]	+	[[Category: Allewell, N M]]
-	[[Category: Cabrera-Luque, J.]]	+	[[Category: Cabrera-Luque, J]]
-	[[Category: Jin, Z.]]	+	[[Category: Jin, Z]]
-	[[Category: Li, Y.]]	+	[[Category: Li, Y]]
-	[[Category: Shi, D.]]	+	[[Category: Shi, D]]
-	[[Category: Tuchman, M.]]	+	[[Category: Tuchman, M]]
-	[[Category: Yu, X.]]	+	[[Category: Yu, X]]
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Synthase]]		[[Category: Synthase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 05:28, 5 June 2014

2014-06-05T05:28:40Z

←Older revision		Revision as of 05:28, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3s7y.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of mmNAGS in Space Group P3121 at 4.3 A resolution==
		+	<StructureSection load='3s7y' size='340' side='right' caption='[[3s7y]], [[Resolution\|resolution]] 4.31Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3s7y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maricaulis_maris Maricaulis maris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S7Y FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6g\|3s6g]], [[3s6h\|3s6h]], [[3s6k\|3s6k]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=74318 Maricaulis maris])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s7y RCSB], [http://www.ebi.ac.uk/pdbsum/3s7y PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Novel bifunctional N-acetylglutamate synthase/kinases (NAGS/K) that catalyze the first two steps of arginine biosynthesis and are homologous to vertebrate N-acetylglutamate synthase (NAGS), an essential cofactor-producing enzyme in the urea cycle, were identified in Maricaulis maris and several other bacteria. Arginine is an allosteric inhibitor of NAGS but not NAGK activity. The crystal structure of M. maris NAGS/K (mmNAGS/K) at 2.7 A resolution indicates that it is a tetramer, in contrast to the hexameric structure of Neisseria gonorrhoeae NAGS. The quaternary structure of crystalline NAGS/K from Xanthomonas campestris (xcNAGS/K) is similar, and cross-linking experiments indicate that both mmNAGS/K and xcNAGS are tetramers in solution. Each subunit has an amino acid kinase (AAK) domain, which is likely responsible for N-acetylglutamate kinase (NAGK) activity and has a putative arginine binding site, and an N-acetyltransferase (NAT) domain that contains the putative NAGS active site. These structures and sequence comparisons suggest that the linker residue 291 may determine whether arginine acts as an allosteric inhibitor or activator in homologous enzymes in microorganisms and vertebrates. In addition, the angle of rotation between AAK and NAT domains varies among crystal forms and subunits within the tetramer. A rotation of 26 degrees is sufficient to close the predicted AcCoA binding site, thus reducing enzymatic activity. Since mmNAGS/K has the highest degree of sequence homology to vertebrate NAGS of NAGS and NAGK enzymes whose structures have been determined, the mmNAGS/K structure was used to develop a structural model of human NAGS that is fully consistent with the functional effects of the 14 missense mutations that were identified in NAGS-deficient patients.

-	<!--	+	A Novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris.,Shi D, Li Y, Cabrera-Luque J, Jin Z, Yu X, Zhao G, Haskins N, Allewell NM, Tuchman M PLoS One. 2011;6(12):e28825. Epub 2011 Dec 12. PMID:22174908<ref>PMID:22174908</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3s7y"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3s7y\| PDB=3s7y \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~mmNAGS in Space Group P3121 at 4.3 A resolution===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22174908}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22174908 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22174908}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3s7y]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Maricaulis_maris Maricaulis maris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022174908<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Maricaulis maris]]		[[Category: Maricaulis maris]]
	[[Category: Allewell, N M.]]		[[Category: Allewell, N M.]]

OCA at 07:51, 18 April 2012

2012-04-18T07:51:47Z

←Older revision		Revision as of 07:51, 18 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3s7y.jpg\|left\|200px]]

-	The ~~entry 3s7y~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3s7y", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3s7y\| PDB=3s7y \| SCENE= }}

-	~~Authors: Shi, D., Li, Y., Cabrera-Luque, J., Jin, Z., Yu, X., Allewell, N.M., Tuchman, M~~.	+	===Crystal structure of mmNAGS in Space Group P3121 at 4.3 A resolution===

-	~~Description~~: ~~Crystal~~ structure ~~of mmNAGS in Space Group P3121 at 4~~.~~3 A resolution~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22174908}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22174908 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22174908}}
		+
		+	==About this Structure==
		+	[[3s7y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maricaulis_maris Maricaulis maris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S7Y OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022174908</ref><references group="xtra"/>
		+	[[Category: Maricaulis maris]]
		+	[[Category: Allewell, N M.]]
		+	[[Category: Cabrera-Luque, J.]]
		+	[[Category: Jin, Z.]]
		+	[[Category: Li, Y.]]
		+	[[Category: Shi, D.]]
		+	[[Category: Tuchman, M.]]
		+	[[Category: Yu, X.]]
		+	[[Category: Kinase]]
		+	[[Category: Synthase]]
		+	[[Category: Transferase]]

OCA at 16:19, 22 June 2011

2011-06-22T16:19:02Z

←Older revision		Revision as of 16:19, 22 June 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3s7y is ON HOLD until ~~sometime in the future~~	+	The entry 3s7y is ON HOLD until Paper Publication

	Authors: Shi, D., Li, Y., Cabrera-Luque, J., Jin, Z., Yu, X., Allewell, N.M., Tuchman, M.		Authors: Shi, D., Li, Y., Cabrera-Luque, J., Jin, Z., Yu, X., Allewell, N.M., Tuchman, M.

	Description: Crystal structure of mmNAGS in Space Group P3121 at 4.3 A resolution		Description: Crystal structure of mmNAGS in Space Group P3121 at 4.3 A resolution

OCA: Protected "3s7y" [edit=sysop:move=sysop]

2011-06-08T04:54:11Z

Protected "3s7y" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:54, 8 June 2011

OCA: New page: '''Unreleased structure''' The entry 3s7y is ON HOLD until sometime in the future Authors: Shi, D., Li, Y., Cabrera-Luque, J., Jin, Z., Yu, X., Allewell, N.M., Tuchman, M. Description:...

2011-06-08T04:54:10Z

New page: '''Unreleased structure''' The entry 3s7y is ON HOLD until sometime in the future Authors: Shi, D., Li, Y., Cabrera-Luque, J., Jin, Z., Yu, X., Allewell, N.M., Tuchman, M. Description:...

New page

'''Unreleased structure'''

The entry 3s7y is ON HOLD until sometime in the future

Authors: Shi, D., Li, Y., Cabrera-Luque, J., Jin, Z., Yu, X., Allewell, N.M., Tuchman, M.

Description: Crystal structure of mmNAGS in Space Group P3121 at 4.3 A resolution