3sge - Revision history

OCA at 12:56, 14 March 2024

2024-03-14T12:56:04Z

←Older revision		Revision as of 12:56, 14 March 2024
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	<StructureSection load='3sge' size='340' side='right'caption='[[3sge]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='3sge' size='340' side='right'caption='[[3sge]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sge]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sge]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SGE FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.89Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3sgd\|3sgd]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sge OCA], [https://pdbe.org/3sge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sge RCSB], [https://www.ebi.ac.uk/pdbsum/3sge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sge ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sge OCA], [https://pdbe.org/3sge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sge RCSB], [https://www.ebi.ac.uk/pdbsum/3sge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sge ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Patients with Chronic Chagas' Heart Disease possess high levels of antibodies against the carboxyl-terminal end of the ribosomal P2ss protein of Trypanosoma cruzi (TcP2ss). These antibodies, as well as the murine monoclonal antibody (mAb) 17.2, recognize the last 13 amino acids of TcP2ss (called the R13 epitope: EEEDDDMGFGLFD) and are able to cross-react with, and stimulate, the ss1 adrenergic receptor (ss1-AR). Indeed, the mAb 17.2 was able to specifically detect human beta1-AR, stably transfected into HEK cells, by flow cytometry and to induce repolarisation abnormalities and first degree atrioventricular conduction block after passive transfer to naive mice. To study the structural basis of this cross-reactivity, we determined the crystal structure of the Fab region of the mAb 17.2 alone at 2.31 A resolution and in complex with the R13 peptide at 1.89 A resolution. We identified as key contact residues on R13 peptide Glu3, Asp6 and Phe9 as was previously shown by alanine scanning. Additionally, we generated a model of human beta1-AR to elucidate the interaction with anti-R13 antibodies. These data provide an understanding of the molecular basis of cross-reactive antibodies induced by chronic infection with Trypanosoma cruzi.

-	Crystal structure of the complex mAb 17.2 and the C-terminal region of Trypanosoma cruzi P2beta protein: implications in cross-reactivity.,Pizarro JC, Boulot G, Bentley GA, Gomez KA, Hoebeke J, Hontebeyrie M, Levin MJ, Smulski CR PLoS Negl Trop Dis. 2011 Nov;5(11):e1375. doi: 10.1371/journal.pntd.0001375. Epub, 2011 Nov 1. PMID:22069505<ref>PMID:22069505</ref>	+	==See Also==
-		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3sge" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Bentley~~, G A~~]]	+	[[Category: Bentley GA]]
-	[[Category: Boulot, G]]	+	[[Category: Boulot G]]
-	[[Category: Hontebeyrie, M]]	+	[[Category: Hontebeyrie M]]
-	[[Category: Pizarro~~, J C]]~~	+	[[Category: Pizarro JC]]
-	~~[[Category: Antigen binding]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin~~]]	+

OCA at 07:55, 29 June 2022

2022-06-29T07:55:09Z

←Older revision		Revision as of 07:55, 29 June 2022
Line 1:		Line 1:

	==Crystal structure of mAb 17.2 in complex with R13 peptide==		==Crystal structure of mAb 17.2 in complex with R13 peptide==
-	<StructureSection load='3sge' size='340' side='right' caption='[[3sge]], [[Resolution\|resolution]] 1.89Å' scene=''>	+	<StructureSection load='3sge' size='340' side='right'caption='[[3sge]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sge]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sge]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SGE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgd\|3sgd]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3sgd\|3sgd]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3sge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sge OCA], [~~http~~://pdbe.org/3sge PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3sge RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3sge PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3sge ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sge OCA], [https://pdbe.org/3sge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sge RCSB], [https://www.ebi.ac.uk/pdbsum/3sge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sge ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Bentley, G A]]		[[Category: Bentley, G A]]

OCA at 11:24, 10 December 2016

2016-12-10T11:24:39Z

←Older revision		Revision as of 11:24, 10 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of mAb 17.2 in complex with R13 peptide==		==Crystal structure of mAb 17.2 in complex with R13 peptide==
	<StructureSection load='3sge' size='340' side='right' caption='[[3sge]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='3sge' size='340' side='right' caption='[[3sge]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sge]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sge]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SGE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgd\|3sgd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgd\|3sgd]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sge RCSB], [http://www.ebi.ac.uk/pdbsum/3sge PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sge OCA], [http://pdbe.org/3sge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3sge RCSB], [http://www.ebi.ac.uk/pdbsum/3sge PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3sge ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3sge" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Bentley, G A]]		[[Category: Bentley, G A]]
	[[Category: Boulot, G]]		[[Category: Boulot, G]]

OCA at 13:32, 9 December 2014

2014-12-09T13:32:55Z

←Older revision		Revision as of 13:32, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3sge.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of mAb 17.2 in complex with R13 peptide==
		+	<StructureSection load='3sge' size='340' side='right' caption='[[3sge]], [[Resolution\|resolution]] 1.89Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3sge]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SGE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgd\|3sgd]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sge RCSB], [http://www.ebi.ac.uk/pdbsum/3sge PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Patients with Chronic Chagas' Heart Disease possess high levels of antibodies against the carboxyl-terminal end of the ribosomal P2ss protein of Trypanosoma cruzi (TcP2ss). These antibodies, as well as the murine monoclonal antibody (mAb) 17.2, recognize the last 13 amino acids of TcP2ss (called the R13 epitope: EEEDDDMGFGLFD) and are able to cross-react with, and stimulate, the ss1 adrenergic receptor (ss1-AR). Indeed, the mAb 17.2 was able to specifically detect human beta1-AR, stably transfected into HEK cells, by flow cytometry and to induce repolarisation abnormalities and first degree atrioventricular conduction block after passive transfer to naive mice. To study the structural basis of this cross-reactivity, we determined the crystal structure of the Fab region of the mAb 17.2 alone at 2.31 A resolution and in complex with the R13 peptide at 1.89 A resolution. We identified as key contact residues on R13 peptide Glu3, Asp6 and Phe9 as was previously shown by alanine scanning. Additionally, we generated a model of human beta1-AR to elucidate the interaction with anti-R13 antibodies. These data provide an understanding of the molecular basis of cross-reactive antibodies induced by chronic infection with Trypanosoma cruzi.

-	~~{{STRUCTURE_3sge\| PDB=3sge \| SCENE= }}~~	+	Crystal structure of the complex mAb 17.2 and the C-terminal region of Trypanosoma cruzi P2beta protein: implications in cross-reactivity.,Pizarro JC, Boulot G, Bentley GA, Gomez KA, Hoebeke J, Hontebeyrie M, Levin MJ, Smulski CR PLoS Negl Trop Dis. 2011 Nov;5(11):e1375. doi: 10.1371/journal.pntd.0001375. Epub, 2011 Nov 1. PMID:22069505<ref>PMID:22069505</ref>

-	~~===Crystal structure~~ of ~~mAb 17~~.~~2 in complex with R13 peptide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22069505}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3sge]] is a 6 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA].~~	+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Bentley, G A.]]	+	[[Category: Bentley, G A]]
-	[[Category: Boulot, G.]]	+	[[Category: Boulot, G]]
-	[[Category: Hontebeyrie, M.]]	+	[[Category: Hontebeyrie, M]]
-	[[Category: Pizarro, J C.]]	+	[[Category: Pizarro, J C]]
	[[Category: Antigen binding]]		[[Category: Antigen binding]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]

OCA at 11:41, 23 January 2013

2013-01-23T11:41:57Z

←Older revision		Revision as of 11:41, 23 January 2013
Line 1:		Line 1:
-	[[Image:3sge.~~jpg~~\|left\|200px]]	+	[[Image:3sge.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3sge", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3sge\| PDB=3sge \| SCENE= }}		{{STRUCTURE_3sge\| PDB=3sge \| SCENE= }}

	===Crystal structure of mAb 17.2 in complex with R13 peptide===		===Crystal structure of mAb 17.2 in complex with R13 peptide===

		+	{{ABSTRACT_PUBMED_22069505}}

	==About this Structure==		==About this Structure==

OCA at 11:48, 9 November 2011

2011-11-09T11:48:50Z

←Older revision		Revision as of 11:48, 9 November 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3sge.jpg\|left\|200px]]

-	The ~~entry 3sge~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3sge", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3sge\| PDB=3sge \| SCENE= }}

-	~~Authors: Pizarro, J.C., Boulot, G., Hontebeyrie, M., Bentley, G.A~~.	+	===Crystal structure of mAb 17.2 in complex with R13 peptide===

-	~~Description: Crystal~~ structure ~~of mAb 17.2 in complex~~ with ~~R13 peptide~~	+
		+	==About this Structure==
		+	[[3sge]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGE OCA].
		+	[[Category: Mus musculus]]
		+	[[Category: Bentley, G A.]]
		+	[[Category: Boulot, G.]]
		+	[[Category: Hontebeyrie, M.]]
		+	[[Category: Pizarro, J C.]]
		+	[[Category: Antigen binding]]
		+	[[Category: Immune system]]
		+	[[Category: Immunoglobulin]]

OCA at 05:47, 17 August 2011

2011-08-17T05:47:49Z

←Older revision		Revision as of 05:47, 17 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3sge is ON HOLD	+	The entry 3sge is ON HOLD until Paper Publication

	Authors: Pizarro, J.C., Boulot, G., Hontebeyrie, M., Bentley, G.A.		Authors: Pizarro, J.C., Boulot, G., Hontebeyrie, M., Bentley, G.A.

	Description: Crystal structure of mAb 17.2 in complex with R13 peptide		Description: Crystal structure of mAb 17.2 in complex with R13 peptide

OCA: Protected "3sge" [edit=sysop:move=sysop]

2011-06-22T16:23:46Z

Protected "3sge" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:23, 22 June 2011

OCA: New page: '''Unreleased structure''' The entry 3sge is ON HOLD Authors: Pizarro, J.C., Boulot, G., Hontebeyrie, M., Bentley, G.A. Description: Crystal structure of mAb 17.2 in complex with R13 p...

2011-06-22T16:23:45Z

New page: '''Unreleased structure''' The entry 3sge is ON HOLD Authors: Pizarro, J.C., Boulot, G., Hontebeyrie, M., Bentley, G.A. Description: Crystal structure of mAb 17.2 in complex with R13 p...

New page

'''Unreleased structure'''

The entry 3sge is ON HOLD

Authors: Pizarro, J.C., Boulot, G., Hontebeyrie, M., Bentley, G.A.

Description: Crystal structure of mAb 17.2 in complex with R13 peptide