3sj7 - Revision history

OCA at 09:51, 1 March 2024

2024-03-01T09:51:00Z

←Older revision		Revision as of 09:51, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3sj7' size='340' side='right'caption='[[3sj7]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3sj7' size='340' side='right'caption='[[3sj7]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Staa8 Staa8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SJ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SJ7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NDP:NADPH+DIHYDRO~~-~~NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>~~, ~~<scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>SAOUHSC_01199 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~93061 STAA8])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj7 OCA], [https://pdbe.org/3sj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sj7 RCSB], [https://www.ebi.ac.uk/pdbsum/3sj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sj7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj7 OCA], [https://pdbe.org/3sj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sj7 RCSB], [https://www.ebi.ac.uk/pdbsum/3sj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sj7 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q2FZ53_STAA8 Q2FZ53_STAA8]
-	Crystal structure of Staphylococcal beta-ketoacyl-ACP reductase 1 (SaFabG1) complexed with NADPH is determined at 2.5 A resolution. The enzyme is essential in FAS-II pathway and utilizes NADPH to reduce beta-ketoacyl-ACP to (S)-beta-hydroxyacyl-ACP. Unlike the tetrameric FabGs, dimeric SaFabG1 shows positive homotropic cooperativity towards NADPH. Analysis of FabG:NADPH binary crystal structure endorses that NADPH interacts directly with the helices alpha4 and alpha5 those are present on a dimerization interface. A steady shift in tryptophan (of alpha4 helix) emission peak upon steady increment of NADPH concentration reveals that the dimeric interface is formed by alpha4-alpha4' and alpha5-alpha5' helices. This dimeric interface imparts positive homotropic cooperativity towards NADPH. PEG, a substrate mimicking molecule is also found near the active site of the enzyme. Proteins 2012. (c) 2012 Wiley-Liss, Inc.	+
-		+
-	Crystal structure and fluorescence studies reveal the role of helical dimeric interface of staphylococcal FabG1 in positive cooperativity for NADPH.,Dutta D, Bhattacharyya S, Das AK Proteins. 2012 Jan 9. doi: 10.1002/~~prot.24024. PMID:22275129<ref>PMID:22275129<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3sj7" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staa8~~]]	+	[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
-	[[Category: Bhattacharyya, S]]	+	[[Category: Bhattacharyya S]]
-	[[Category: Das~~, A K~~]]	+	[[Category: Das AK]]
-	[[Category: Dutta, D]]	+	[[Category: Dutta D]]
-	~~[[Category: Ketoreductase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 07:58, 29 June 2022

2022-06-29T07:58:12Z

←Older revision		Revision as of 07:58, 29 June 2022
Line 1:		Line 1:

	==Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH==		==Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH==
-	<StructureSection load='3sj7' size='340' side='right' caption='[[3sj7]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3sj7' size='340' side='right'caption='[[3sj7]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sj7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staa8 Staa8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SJ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staa8 Staa8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SJ7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_01199 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_01199 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj7 OCA], [~~http~~://pdbe.org/3sj7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3sj7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3sj7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3sj7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj7 OCA], [https://pdbe.org/3sj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sj7 RCSB], [https://www.ebi.ac.uk/pdbsum/3sj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sj7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staa8]]		[[Category: Staa8]]
	[[Category: Bhattacharyya, S]]		[[Category: Bhattacharyya, S]]

OCA at 11:19, 11 August 2016

2016-08-11T11:19:11Z

←Older revision		Revision as of 11:19, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH==		==Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH==
	<StructureSection load='3sj7' size='340' side='right' caption='[[3sj7]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3sj7' size='340' side='right' caption='[[3sj7]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sj7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SJ7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sj7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staa8 Staa8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SJ7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_01199 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~46170 Staphylococcus aureus subsp. aureus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_01199 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sj7 RCSB], [http://www.ebi.ac.uk/pdbsum/3sj7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj7 OCA], [http://pdbe.org/3sj7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3sj7 RCSB], [http://www.ebi.ac.uk/pdbsum/3sj7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3sj7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3sj7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Staphylococcus aureus subsp. aureus~~]]	+	[[Category: Staa8]]
	[[Category: Bhattacharyya, S]]		[[Category: Bhattacharyya, S]]
	[[Category: Das, A K]]		[[Category: Das, A K]]

OCA at 09:59, 4 January 2015

2015-01-04T09:59:24Z

←Older revision		Revision as of 09:59, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3sj7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SJ7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3sj7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SJ7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_01199 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_01199 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sj7 RCSB], [http://www.ebi.ac.uk/pdbsum/3sj7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sj7 RCSB], [http://www.ebi.ac.uk/pdbsum/3sj7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Crystal structure and fluorescence studies reveal the role of helical dimeric interface of staphylococcal FabG1 in positive cooperativity for NADPH.,Dutta D, Bhattacharyya S, Das AK Proteins. 2012 Jan 9. doi: 10.1002/prot.24024. PMID:22275129<ref>PMID:22275129</ref>		Crystal structure and fluorescence studies reveal the role of helical dimeric interface of staphylococcal FabG1 in positive cooperativity for NADPH.,Dutta D, Bhattacharyya S, Das AK Proteins. 2012 Jan 9. doi: 10.1002/prot.24024. PMID:22275129<ref>PMID:22275129</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
-	[[Category: Bhattacharyya, S.]]	+	[[Category: Bhattacharyya, S]]
-	[[Category: Das, A K.]]	+	[[Category: Das, A K]]
-	[[Category: Dutta, D.]]	+	[[Category: Dutta, D]]
	[[Category: Ketoreductase]]		[[Category: Ketoreductase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]

OCA at 05:24, 5 June 2014

2014-06-05T05:24:53Z

←Older revision		Revision as of 05:24, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3sj7.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH==
		+	<StructureSection load='3sj7' size='340' side='right' caption='[[3sj7]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3sj7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SJ7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAOUHSC_01199 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sj7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sj7 RCSB], [http://www.ebi.ac.uk/pdbsum/3sj7 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Crystal structure of Staphylococcal beta-ketoacyl-ACP reductase 1 (SaFabG1) complexed with NADPH is determined at 2.5 A resolution. The enzyme is essential in FAS-II pathway and utilizes NADPH to reduce beta-ketoacyl-ACP to (S)-beta-hydroxyacyl-ACP. Unlike the tetrameric FabGs, dimeric SaFabG1 shows positive homotropic cooperativity towards NADPH. Analysis of FabG:NADPH binary crystal structure endorses that NADPH interacts directly with the helices alpha4 and alpha5 those are present on a dimerization interface. A steady shift in tryptophan (of alpha4 helix) emission peak upon steady increment of NADPH concentration reveals that the dimeric interface is formed by alpha4-alpha4' and alpha5-alpha5' helices. This dimeric interface imparts positive homotropic cooperativity towards NADPH. PEG, a substrate mimicking molecule is also found near the active site of the enzyme. Proteins 2012. (c) 2012 Wiley-Liss, Inc.

-	~~<!--~~	+	Crystal structure and fluorescence studies reveal the role of helical dimeric interface of staphylococcal FabG1 in positive cooperativity for NADPH.,Dutta D, Bhattacharyya S, Das AK Proteins. 2012 Jan 9. doi: 10.1002/prot.24024. PMID:22275129<ref>PMID:22275129</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3sj7", creates~~ the ~~"Structure Box" on the page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3sj7\| PDB=3sj7 \| SCENE= }}~~	+

-	~~===Structure~~ of ~~beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22275129}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22275129 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22275129}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3sj7]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022275129<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
	[[Category: Bhattacharyya, S.]]		[[Category: Bhattacharyya, S.]]

OCA at 05:39, 8 February 2012

2012-02-08T05:39:40Z

←Older revision		Revision as of 05:39, 8 February 2012
Line 11:		Line 11:
	===Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH===		===Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22275129}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22275129 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22275129}}

	==About this Structure==		==About this Structure==
	[[3sj7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA].		[[3sj7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022275129</ref><references group="xtra"/>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
	[[Category: Bhattacharyya, S.]]		[[Category: Bhattacharyya, S.]]

OCA at 07:28, 1 February 2012

2012-02-01T07:28:12Z

←Older revision		Revision as of 07:28, 1 February 2012
Line 1:		Line 1:
-	[[Image:3sj7.~~jpg~~\|left\|200px]]	+	[[Image:3sj7.png\|left\|200px]]

	<!--		<!--

OCA at 06:43, 25 January 2012

2012-01-25T06:43:34Z

←Older revision		Revision as of 06:43, 25 January 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3sj7.jpg\|left\|200px]]

-	The ~~entry 3sj7~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3sj7", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3sj7\| PDB=3sj7 \| SCENE= }}

-	~~Authors: Dutta, D., Bhattacharyya, S., Das, A.K.~~	+	===Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH===

-	~~Description:~~ Structure ~~of beta-ketoacetyl-CoA reductase (FabG)~~ from Staphylococcus aureus ~~complex with NADPH~~	+
		+	==About this Structure==
		+	[[3sj7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SJ7 OCA].
		+	[[Category: Staphylococcus aureus subsp. aureus]]
		+	[[Category: Bhattacharyya, S.]]
		+	[[Category: Das, A K.]]
		+	[[Category: Dutta, D.]]
		+	[[Category: Ketoreductase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rossmann fold]]

OCA at 18:26, 6 July 2011

2011-07-06T18:26:45Z

←Older revision		Revision as of 18:26, 6 July 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3sj7 is ON HOLD	+	The entry 3sj7 is ON HOLD until Paper Publication

-	Authors: Dutta, D, Bhattacharyya, S, Das, A.K.	+	Authors: Dutta, D., Bhattacharyya, S., Das, A.K.

	Description: Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH		Description: Structure of beta-ketoacetyl-CoA reductase (FabG) from Staphylococcus aureus complex with NADPH

OCA: Protected "3sj7" [edit=sysop:move=sysop]

2011-06-29T05:46:36Z

Protected "3sj7" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:46, 29 June 2011