3srp - Revision history

OCA at 13:05, 14 March 2024

2024-03-14T13:05:53Z

←Older revision		Revision as of 13:05, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3srp' size='340' side='right'caption='[[3srp]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='3srp' size='340' side='right'caption='[[3srp]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3srp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Castor_bean Castor bean~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3bjg 3bjg]. The May 2013 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ricin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2013_5 10.2210/rcsb_pdb/mom_2013_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SRP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3srp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3bjg 3bjg]. The May 2013 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ricin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2013_5 10.2210/rcsb_pdb/mom_2013_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SRP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.14Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3bjg\|3bjg]], [[3lc9\|3lc9]], [[3mk9\|3mk9]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [https://pdbe.org/3srp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [https://www.ebi.ac.uk/pdbsum/3srp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3srp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [https://pdbe.org/3srp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [https://www.ebi.ac.uk/pdbsum/3srp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3srp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO]] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).	+	[https://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	RiVax is a recombinant protein that is currently under clinical development as part of a human vaccine to protect against ricin poisoning. RiVax includes ricin A-chain (RTA) residues 1-267 with two intentional amino-acid substitutions, V76M and Y80A, aimed at reducing toxicity. Here, the crystal structure of RiVax was solved to 2.1 A resolution and it was shown that it is superposable with that of the ricin toxin A-chain from Ricinus communis with a root-mean-square deviation of 0.6 A over 258 C(alpha) atoms. The RiVax structure is also compared with the recently determined structure of another potential ricin-vaccine immunogen, RTA 1-33/44-198 R48C/T77C. Finally, the locations and solvent-exposure of two toxin-neutralizing B-cell epitopes were examined and it was found that these epitopes are within or near regions predicted to be involved in catalysis. The results demonstrate the composition of the RiVax clinical material and will guide ongoing protein-engineering strategies to develop improved immunogens.	+
-		+
-	Structure of RiVax: a recombinant ricin vaccine.,Legler PM, Brey RN, Smallshaw JE, Vitetta ES, Millard CB Acta Crystallogr D Biol Crystallogr. 2011 Sep;67(Pt 9):826-30. doi:, 10.1107/S0907444911026771. Epub 2011 Aug 9. PMID:21904036<ref>PMID:21904036</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3srp" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Ricin 3D structures\|Ricin 3D structures]]		*[[Ricin 3D structures\|Ricin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Castor bean]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Ricin]]		[[Category: Ricin]]
-	[[Category: ~~RRNA N-glycosylase~~]]	+	[[Category: Ricinus communis]]
-	[[Category: Legler~~, P M~~]]	+	[[Category: Legler PM]]
-	[[Category: Millard~~, C B]]~~	+	[[Category: Millard CB]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Immunogen]]~~	+
-	~~[[Category: Ribosome inactivating protein~~]]	+

OCA at 08:07, 29 June 2022

2022-06-29T08:07:44Z

←Older revision		Revision as of 08:07, 29 June 2022
Line 1:		Line 1:

	==Structure of Rivax: A Human Ricin Vaccine==		==Structure of Rivax: A Human Ricin Vaccine==
-	<StructureSection load='3srp' size='340' side='right' caption='[[3srp]], [[Resolution\|resolution]] 2.14Å' scene=''>	+	<StructureSection load='3srp' size='340' side='right'caption='[[3srp]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3srp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Castor_bean Castor bean]. The May 2013 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ricin'' by David Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2013_5 10.2210/rcsb_pdb/mom_2013_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=3SRP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3srp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Castor_bean Castor bean]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3bjg 3bjg]. The May 2013 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ricin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2013_5 10.2210/rcsb_pdb/mom_2013_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SRP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bjg\|3bjg]], [[3lc9\|3lc9]], [[3mk9\|3mk9]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bjg\|3bjg]], [[3lc9\|3lc9]], [[3mk9\|3mk9]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [~~http~~://pdbe.org/3srp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3srp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3srp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [https://pdbe.org/3srp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [https://www.ebi.ac.uk/pdbsum/3srp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3srp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO]] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).	+	[[https://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO]] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Ricin\|Ricin]]	+	*[[Ricin 3D structures\|Ricin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Castor bean]]		[[Category: Castor bean]]
		+	[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Ricin]]		[[Category: Ricin]]

OCA at 09:44, 5 August 2016

2016-08-05T09:44:23Z

←Older revision		Revision as of 09:44, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of Rivax: A Human Ricin Vaccine==		==Structure of Rivax: A Human Ricin Vaccine==
	<StructureSection load='3srp' size='340' side='right' caption='[[3srp]], [[Resolution\|resolution]] 2.14Å' scene=''>		<StructureSection load='3srp' size='340' side='right' caption='[[3srp]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3srp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Ricinus_communis Ricinus communis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SRP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3srp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Castor_bean Castor bean]. The May 2013 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ricin'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2013_5 10.2210/rcsb_pdb/mom_2013_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SRP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bjg\|3bjg]], [[3lc9\|3lc9]], [[3mk9\|3mk9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bjg\|3bjg]], [[3lc9\|3lc9]], [[3mk9\|3mk9]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [http://www.ebi.ac.uk/pdbsum/3srp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [http://pdbe.org/3srp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [http://www.ebi.ac.uk/pdbsum/3srp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3srp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3srp" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ricinus communis~~]]	+	[[Category: Castor bean]]
		+	[[Category: RCSB PDB Molecule of the Month]]
		+	[[Category: Ricin]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Legler, P M]]		[[Category: Legler, P M]]

OCA at 01:17, 25 December 2014

2014-12-25T01:17:03Z

←Older revision		Revision as of 01:17, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [http://www.ebi.ac.uk/pdbsum/3srp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [http://www.ebi.ac.uk/pdbsum/3srp PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO]] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 13:01, 9 December 2014

2014-12-09T13:01:42Z

←Older revision		Revision as of 13:01, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3srp.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of Rivax: A Human Ricin Vaccine==
		+	<StructureSection load='3srp' size='340' side='right' caption='[[3srp]], [[Resolution\|resolution]] 2.14Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3srp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SRP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bjg\|3bjg]], [[3lc9\|3lc9]], [[3mk9\|3mk9]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3srp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3srp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3srp RCSB], [http://www.ebi.ac.uk/pdbsum/3srp PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RiVax is a recombinant protein that is currently under clinical development as part of a human vaccine to protect against ricin poisoning. RiVax includes ricin A-chain (RTA) residues 1-267 with two intentional amino-acid substitutions, V76M and Y80A, aimed at reducing toxicity. Here, the crystal structure of RiVax was solved to 2.1 A resolution and it was shown that it is superposable with that of the ricin toxin A-chain from Ricinus communis with a root-mean-square deviation of 0.6 A over 258 C(alpha) atoms. The RiVax structure is also compared with the recently determined structure of another potential ricin-vaccine immunogen, RTA 1-33/44-198 R48C/T77C. Finally, the locations and solvent-exposure of two toxin-neutralizing B-cell epitopes were examined and it was found that these epitopes are within or near regions predicted to be involved in catalysis. The results demonstrate the composition of the RiVax clinical material and will guide ongoing protein-engineering strategies to develop improved immunogens.

-	~~{{STRUCTURE_3srp\| PDB=3srp \| SCENE= }}~~	+	Structure of RiVax: a recombinant ricin vaccine.,Legler PM, Brey RN, Smallshaw JE, Vitetta ES, Millard CB Acta Crystallogr D Biol Crystallogr. 2011 Sep;67(Pt 9):826-30. doi:, 10.1107/S0907444911026771. Epub 2011 Aug 9. PMID:21904036<ref>PMID:21904036</ref>

-	~~===Structure~~ of ~~Rivax: A Human Ricin Vaccine===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21904036}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3srp]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Ricinus_communis Ricinus communis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA].~~	+

	==See Also==		==See Also==
	*[[Ricin\|Ricin]]		*[[Ricin\|Ricin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021904036<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Ricinus communis]]		[[Category: Ricinus communis]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
-	[[Category: Legler, P M.]]	+	[[Category: Legler, P M]]
-	[[Category: Millard, C B.]]	+	[[Category: Millard, C B]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Immunogen]]		[[Category: Immunogen]]
	[[Category: Ribosome inactivating protein]]		[[Category: Ribosome inactivating protein]]

OCA at 11:03, 20 October 2012

2012-10-20T11:03:35Z

←Older revision		Revision as of 11:03, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3srp]] is a 1 chain structure ~~of [[Ricin]]~~ with sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA].	+	[[3srp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA].

	==See Also==		==See Also==

OCA at 04:06, 26 July 2012

2012-07-26T04:06:41Z

←Older revision		Revision as of 04:06, 26 July 2012
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	[[Image:3srp.png\|left\|200px]]		[[Image:3srp.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3srp", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3srp\| PDB=3srp \| SCENE= }}		{{STRUCTURE_3srp\| PDB=3srp \| SCENE= }}

	===Structure of Rivax: A Human Ricin Vaccine===		===Structure of Rivax: A Human Ricin Vaccine===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21904036}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21904036 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21904036}}		{{ABSTRACT_PUBMED_21904036}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Ricin]]	+	*[[Ricin\|Ricin]]

	==Reference==		==Reference==

OCA at 05:41, 11 January 2012

2012-01-11T05:41:51Z

←Older revision		Revision as of 05:41, 11 January 2012
Line 1:		Line 1:
-	[[Image:3srp.~~jpg~~\|left\|200px]]	+	[[Image:3srp.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Structure of Rivax: A Human Ricin Vaccine===		===Structure of Rivax: A Human Ricin Vaccine===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21904036}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21904036 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21904036}}

	==About this Structure==		==About this Structure==
-	[[3srp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA].	+	[[3srp]] is a 1 chain structure of [[Ricin]] with sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA].
		+
		+	==See Also==
		+	*[[Ricin]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021904036</ref><references group="xtra"/>
	[[Category: Ricinus communis]]		[[Category: Ricinus communis]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]

OCA at 09:03, 3 August 2011

2011-08-03T09:03:26Z

←Older revision		Revision as of 09:03, 3 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3srp.jpg\|left\|200px]]

-	The ~~entry 3srp~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3srp", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3srp\| PDB=3srp \| SCENE= }}

-	~~Authors~~: ~~Legler, P.M., Millard, C.B.~~	+	===Structure of Rivax: A Human Ricin Vaccine===

-	~~Description:~~ Structure ~~of Rivax~~: ~~A Human Ricin Vaccine~~	+
		+	==About this Structure==
		+	[[3srp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SRP OCA].
		+	[[Category: Ricinus communis]]
		+	[[Category: RRNA N-glycosylase]]
		+	[[Category: Legler, P M.]]
		+	[[Category: Millard, C B.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Immunogen]]
		+	[[Category: Ribosome inactivating protein]]

OCA: Protected "3srp" [edit=sysop:move=sysop]

2011-07-13T06:56:03Z

Protected "3srp" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:56, 13 July 2011