3svl - Revision history

OCA at 13:08, 14 March 2024

2024-03-14T13:08:48Z

←Older revision		Revision as of 13:08, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3svl' size='340' side='right'caption='[[3svl]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3svl' size='340' side='right'caption='[[3svl]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SVL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SVL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~yieF, b3713, JW3691 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83333 ECOLI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [https://pdbe.org/3svl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [https://www.ebi.ac.uk/pdbsum/3svl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3svl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [https://pdbe.org/3svl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [https://www.ebi.ac.uk/pdbsum/3svl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3svl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CHRR_ECOLI CHRR_ECOLI]] Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr(6+) producing Cr(3+) and one electron to molecular oxygen without producing the toxic Cr(5+) species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V(5+), Mo(6+), methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.<ref>PMID:14766567</ref> <ref>PMID:16621832</ref> <ref>PMID:17088379</ref> <ref>PMID:22558308</ref>	+	[https://www.uniprot.org/uniprot/CHRR_ECOLI CHRR_ECOLI] Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr(6+) producing Cr(3+) and one electron to molecular oxygen without producing the toxic Cr(5+) species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V(5+), Mo(6+), methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.<ref>PMID:14766567</ref> <ref>PMID:16621832</ref> <ref>PMID:17088379</ref> <ref>PMID:22558308</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The Escherichia coli ChrR enzyme is an obligatory two-electron quinone reductase that has many applications, such as in chromate bioremediation. Its crystal structure, solved at 2.2 A resolution, shows that it belongs to the flavodoxin superfamily in which flavin mononucleotide (FMN) is firmly anchored to the protein. ChrR crystallized as a tetramer, and size exclusion chromatography showed that this is the oligomeric form that catalyzes chromate reduction. Within the tetramer, the dimers interact by a pair of two hydrogen bond networks, each involving Tyr128 and Glu146 of one dimer and Arg125 and Tyr85 of the other; the latter extends to one of the redox FMN cofactors. Changes in each of these amino acids enhanced chromate reductase activity of the enzyme, showing that this network is centrally involved in chromate reduction.	+
-		+
-	Crystal Structure of ChrR-A Quinone Reductase with the Capacity to Reduce Chromate.,Eswaramoorthy S, Poulain S, Hienerwadel R, Bremond N, Sylvester MD, Zhang YB, Berthomieu C, Van Der Lelie D, Matin A PLoS One. 2012;7(4):e36017. Epub 2012 Apr 27. PMID:22558308<ref>PMID:22558308</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3svl" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Berthomieu, C]]	+	[[Category: Berthomieu C]]
-	[[Category: Bremond, N]]	+	[[Category: Bremond N]]
-	[[Category: Eswaramoorthy, S]]	+	[[Category: Eswaramoorthy S]]
-	[[Category: Hienerwadel, R]]	+	[[Category: Hienerwadel R]]
-	~~[[Category: Lelie, D Van Der~~]]	+	[[Category: Matin AC]]
-	[[Category: Matin~~, A C~~]]	+	[[Category: Poulain S]]
-	[[Category: Poulain, S]]	+	[[Category: Sylvester MD]]
-	[[Category: Sylvester~~, M D~~]]	+	[[Category: Van Der Lelie D]]
-	[[Category: ~~Zhang, Y B~~]]	+	[[Category: Zhang YB]]
-	[[Category: ~~Chromate bioremediation]]~~	+
-	~~[[Category: E. coli chrr enzyme]]~~	+
-	~~[[Category: Improved mutant enzyme]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Tetramer role~~]]	+

OCA at 08:12, 29 June 2022

2022-06-29T08:12:28Z

←Older revision		Revision as of 08:12, 29 June 2022
Line 1:		Line 1:

	==Structural basis of the improvement of ChrR - a multi-purpose enzyme==		==Structural basis of the improvement of ChrR - a multi-purpose enzyme==
-	<StructureSection load='3svl' size='340' side='right' caption='[[3svl]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='3svl' size='340' side='right'caption='[[3svl]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SVL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SVL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yieF, b3713, JW3691 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yieF, b3713, JW3691 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [~~http~~://pdbe.org/3svl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3svl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3svl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [https://pdbe.org/3svl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [https://www.ebi.ac.uk/pdbsum/3svl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3svl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CHRR_ECOLI CHRR_ECOLI]] Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr(6+) producing Cr(3+) and one electron to molecular oxygen without producing the toxic Cr(5+) species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V(5+), Mo(6+), methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.<ref>PMID:14766567</ref> <ref>PMID:16621832</ref> <ref>PMID:17088379</ref> <ref>PMID:22558308</ref>	+	[[https://www.uniprot.org/uniprot/CHRR_ECOLI CHRR_ECOLI]] Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr(6+) producing Cr(3+) and one electron to molecular oxygen without producing the toxic Cr(5+) species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V(5+), Mo(6+), methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.<ref>PMID:14766567</ref> <ref>PMID:16621832</ref> <ref>PMID:17088379</ref> <ref>PMID:22558308</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Berthomieu, C]]		[[Category: Berthomieu, C]]
	[[Category: Bremond, N]]		[[Category: Bremond, N]]

OCA at 19:26, 11 August 2016

2016-08-11T19:26:35Z

←Older revision		Revision as of 19:26, 11 August 2016
Line 1:		Line 1:
		+
	==Structural basis of the improvement of ChrR - a multi-purpose enzyme==		==Structural basis of the improvement of ChrR - a multi-purpose enzyme==
	<StructureSection load='3svl' size='340' side='right' caption='[[3svl]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3svl' size='340' side='right' caption='[[3svl]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SVL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SVL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yieF, b3713, JW3691 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yieF, b3713, JW3691 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [http://www.ebi.ac.uk/pdbsum/3svl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [http://pdbe.org/3svl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [http://www.ebi.ac.uk/pdbsum/3svl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3svl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHRR_ECOLI CHRR_ECOLI]] Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr(6+) producing Cr(3+) and one electron to molecular oxygen without producing the toxic Cr(5+) species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V(5+), Mo(6+), methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.<ref>PMID:14766567</ref> <ref>PMID:16621832</ref> <ref>PMID:17088379</ref> <ref>PMID:22558308</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3svl" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Berthomieu, C]]		[[Category: Berthomieu, C]]
	[[Category: Bremond, N]]		[[Category: Bremond, N]]

OCA at 11:04, 4 January 2015

2015-01-04T11:04:15Z

←Older revision		Revision as of 11:04, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SVL FirstGlance]. <br>		<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SVL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yieF, b3713, JW3691 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yieF, b3713, JW3691 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [http://www.ebi.ac.uk/pdbsum/3svl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [http://www.ebi.ac.uk/pdbsum/3svl PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Crystal Structure of ChrR-A Quinone Reductase with the Capacity to Reduce Chromate.,Eswaramoorthy S, Poulain S, Hienerwadel R, Bremond N, Sylvester MD, Zhang YB, Berthomieu C, Van Der Lelie D, Matin A PLoS One. 2012;7(4):e36017. Epub 2012 Apr 27. PMID:22558308<ref>PMID:22558308</ref>		Crystal Structure of ChrR-A Quinone Reductase with the Capacity to Reduce Chromate.,Eswaramoorthy S, Poulain S, Hienerwadel R, Bremond N, Sylvester MD, Zhang YB, Berthomieu C, Van Der Lelie D, Matin A PLoS One. 2012;7(4):e36017. Epub 2012 Apr 27. PMID:22558308<ref>PMID:22558308</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Berthomieu, C.]]	+	[[Category: Berthomieu, C]]
-	[[Category: Bremond, N.]]	+	[[Category: Bremond, N]]
-	[[Category: Eswaramoorthy, S.]]	+	[[Category: Eswaramoorthy, S]]
-	[[Category: Hienerwadel, R.]]	+	[[Category: Hienerwadel, R]]
-	[[Category: Lelie, D Van Der.]]	+	[[Category: Lelie, D Van Der]]
-	[[Category: Matin, A C.]]	+	[[Category: Matin, A C]]
-	[[Category: Poulain, S.]]	+	[[Category: Poulain, S]]
-	[[Category: Sylvester, M D.]]	+	[[Category: Sylvester, M D]]
-	[[Category: Zhang, Y B.]]	+	[[Category: Zhang, Y B]]
	[[Category: Chromate bioremediation]]		[[Category: Chromate bioremediation]]
	[[Category: E. coli chrr enzyme]]		[[Category: E. coli chrr enzyme]]

OCA at 05:28, 5 June 2014

2014-06-05T05:28:41Z

←Older revision		Revision as of 05:28, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3svl.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Structural basis of the improvement of ChrR - a multi-purpose enzyme==
		+	<StructureSection load='3svl' size='340' side='right' caption='[[3svl]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SVL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yieF, b3713, JW3691 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [http://www.ebi.ac.uk/pdbsum/3svl PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Escherichia coli ChrR enzyme is an obligatory two-electron quinone reductase that has many applications, such as in chromate bioremediation. Its crystal structure, solved at 2.2 A resolution, shows that it belongs to the flavodoxin superfamily in which flavin mononucleotide (FMN) is firmly anchored to the protein. ChrR crystallized as a tetramer, and size exclusion chromatography showed that this is the oligomeric form that catalyzes chromate reduction. Within the tetramer, the dimers interact by a pair of two hydrogen bond networks, each involving Tyr128 and Glu146 of one dimer and Arg125 and Tyr85 of the other; the latter extends to one of the redox FMN cofactors. Changes in each of these amino acids enhanced chromate reductase activity of the enzyme, showing that this network is centrally involved in chromate reduction.

-	<!--	+	Crystal Structure of ChrR-A Quinone Reductase with the Capacity to Reduce Chromate.,Eswaramoorthy S, Poulain S, Hienerwadel R, Bremond N, Sylvester MD, Zhang YB, Berthomieu C, Van Der Lelie D, Matin A PLoS One. 2012;7(4):e36017. Epub 2012 Apr 27. PMID:22558308<ref>PMID:22558308</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3svl"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3svl\| PDB=3svl \| SCENE= }}~~	+

-	~~===Structural basis~~ of the ~~improvement of ChrR - a multi-purpose enzyme===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22558308}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22558308 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22558308}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3svl]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022558308<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Berthomieu, C.]]		[[Category: Berthomieu, C.]]

OCA at 05:53, 30 May 2012

2012-05-30T05:53:57Z

←Older revision		Revision as of 05:53, 30 May 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3svl.jpg\|left\|200px]]

-	The ~~entry 3svl~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3svl", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3svl\| PDB=3svl \| SCENE= }}

-	~~Authors: Poulain, S., Eswaramoorthy, S., Hienerwadel, R., Bremond, N., Sylvester, M.D., Zhang, Y.B., Van Der Lelie, D., Berthomieu, C., Matin, A.C.~~	+	===Structural basis of the improvement of ChrR - a multi-purpose enzyme===

-	~~Description~~: ~~Structural basis of~~ the ~~improvement of ChrR~~ -a ~~multi~~-~~purpose~~ enzyme	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22558308}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22558308 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22558308}}
		+
		+	==About this Structure==
		+	[[3svl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022558308</ref><references group="xtra"/>
		+	[[Category: Escherichia coli]]
		+	[[Category: Berthomieu, C.]]
		+	[[Category: Bremond, N.]]
		+	[[Category: Eswaramoorthy, S.]]
		+	[[Category: Hienerwadel, R.]]
		+	[[Category: Lelie, D Van Der.]]
		+	[[Category: Matin, A C.]]
		+	[[Category: Poulain, S.]]
		+	[[Category: Sylvester, M D.]]
		+	[[Category: Zhang, Y B.]]
		+	[[Category: Chromate bioremediation]]
		+	[[Category: E. coli chrr enzyme]]
		+	[[Category: Improved mutant enzyme]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Tetramer role]]

OCA at 05:43, 7 September 2011

2011-09-07T05:43:26Z

←Older revision		Revision as of 05:43, 7 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3svl is ON HOLD	+	The entry 3svl is ON HOLD until Paper Publication

	Authors: Poulain, S., Eswaramoorthy, S., Hienerwadel, R., Bremond, N., Sylvester, M.D., Zhang, Y.B., Van Der Lelie, D., Berthomieu, C., Matin, A.C.		Authors: Poulain, S., Eswaramoorthy, S., Hienerwadel, R., Bremond, N., Sylvester, M.D., Zhang, Y.B., Van Der Lelie, D., Berthomieu, C., Matin, A.C.

	Description: Structural basis of the improvement of ChrR -a multi-purpose enzyme		Description: Structural basis of the improvement of ChrR -a multi-purpose enzyme

OCA: Protected "3svl" [edit=sysop:move=sysop]

2011-07-20T06:18:21Z

Protected "3svl" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:18, 20 July 2011

OCA: New page: '''Unreleased structure''' The entry 3svl is ON HOLD Authors: Poulain, S., Eswaramoorthy, S., Hienerwadel, R., Bremond, N., Sylvester, M.D., Zhang, Y.B., Van Der Lelie, D., Berthomieu, ...

2011-07-20T06:18:19Z

New page: '''Unreleased structure''' The entry 3svl is ON HOLD Authors: Poulain, S., Eswaramoorthy, S., Hienerwadel, R., Bremond, N., Sylvester, M.D., Zhang, Y.B., Van Der Lelie, D., Berthomieu, ...

New page

'''Unreleased structure'''

The entry 3svl is ON HOLD

Authors: Poulain, S., Eswaramoorthy, S., Hienerwadel, R., Bremond, N., Sylvester, M.D., Zhang, Y.B., Van Der Lelie, D., Berthomieu, C., Matin, A.C.

Description: Structural basis of the improvement of ChrR -a multi-purpose enzyme