3sxa - Revision history

OCA at 13:10, 14 March 2024

2024-03-14T13:10:57Z

←Older revision		Revision as of 13:10, 14 March 2024
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	<StructureSection load='3sxa' size='340' side='right'caption='[[3sxa]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='3sxa' size='340' side='right'caption='[[3sxa]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sxa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SXA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sxa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SXA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3sx3\|3sx3]], [[3sx5\|3sx5]], [[3sx7\|3sx7]], [[3sx8\|3sx8]], [[3sxb\|3sxb]], [[3sxc\|3sxc]], [[3sxd\|3sxd]], [[3sxe\|3sxe]], [[3sxg\|3sxg]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sxa OCA], [https://pdbe.org/3sxa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sxa RCSB], [https://www.ebi.ac.uk/pdbsum/3sxa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sxa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sxa OCA], [https://pdbe.org/3sxa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sxa RCSB], [https://www.ebi.ac.uk/pdbsum/3sxa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sxa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BGAT_HUMAN BGAT_HUMAN]] This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.	+	[https://www.uniprot.org/uniprot/BGAT_HUMAN BGAT_HUMAN] This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The human ABO(H) A and B blood group glycosyltransferases GTA and GTB differ by only four amino acids, yet this small dissimilarity is responsible for significant differences in biosynthesis, kinetics and structure. Like other glycosyltransferases, these two enzymes have been shown to recognize substrates through dramatic conformational changes in mobile polypeptide loops surrounding the active site. Structures of GTA, GTB and several chimeras determined by single-crystal X-ray diffraction demonstrate a range of susceptibility to the choice of cryoprotectant, in which the mobile polypeptide loops can be induced by glycerol to form the ordered closed conformation associated with substrate recognition and by MPD [hexylene glycol, (+/-)-2-methyl-2,4-pentanediol] to hinder binding of substrate in the active site owing to chelation of the Mn(2+) cofactor and thereby adopt the disordered open state. Glycerol is often avoided as a cryoprotectant when determining the structures of carbohydrate-active enzymes as it may act as a competitive inhibitor for monosaccharide ligands. Here, it is shown that the use of glycerol as a cryoprotectant can additionally induce significant changes in secondary structure, a phenomenon that could apply to any class of protein.	+
-		+
-	Sequence-dependent effects of cryoprotectants on the active sites of the human ABO(H) blood group A and B glycosyltransferases.,Johal AR, Schuman B, Alfaro JA, Borisova S, Seto NO, Evans SV Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):268-76. Epub 2012 Feb 14. PMID:22349229<ref>PMID:22349229</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3sxa" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]		*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Fucosylgalactoside 3-alpha-galactosyltransferase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Evans~~, S V~~]]	+	[[Category: Evans SV]]
-	[[Category: Johal~~, A R]]~~	+	[[Category: Johal AR]]
-	~~[[Category: Abo rossmann fold]]~~	+
-	~~[[Category: Blood group antigen]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Manganese]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Retaining glycosyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:14, 29 June 2022

2022-06-29T08:14:29Z

←Older revision		Revision as of 08:14, 29 June 2022
Line 1:		Line 1:

	==Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant==		==Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant==
-	<StructureSection load='3sxa' size='340' side='right' caption='[[3sxa]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='3sxa' size='340' side='right'caption='[[3sxa]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sxa]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SXA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sxa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SXA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sx3\|3sx3]], [[3sx5\|3sx5]], [[3sx7\|3sx7]], [[3sx8\|3sx8]], [[3sxb\|3sxb]], [[3sxc\|3sxc]], [[3sxd\|3sxd]], [[3sxe\|3sxe]], [[3sxg\|3sxg]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3sx3\|3sx3]], [[3sx5\|3sx5]], [[3sx7\|3sx7]], [[3sx8\|3sx8]], [[3sxb\|3sxb]], [[3sxc\|3sxc]], [[3sxd\|3sxd]], [[3sxe\|3sxe]], [[3sxg\|3sxg]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3sxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sxa OCA], [~~http~~://pdbe.org/3sxa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3sxa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3sxa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3sxa ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sxa OCA], [https://pdbe.org/3sxa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sxa RCSB], [https://www.ebi.ac.uk/pdbsum/3sxa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sxa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BGAT_HUMAN BGAT_HUMAN]] This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.	+	[[https://www.uniprot.org/uniprot/BGAT_HUMAN BGAT_HUMAN]] This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 3sxa" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3sxa" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 30:
	[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]		[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Evans, S V]]		[[Category: Evans, S V]]
	[[Category: Johal, A R]]		[[Category: Johal, A R]]

OCA at 10:42, 4 August 2016

2016-08-04T10:42:40Z

←Older revision		Revision as of 10:42, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant==		==Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant==
	<StructureSection load='3sxa' size='340' side='right' caption='[[3sxa]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='3sxa' size='340' side='right' caption='[[3sxa]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sxa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SXA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sxa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SXA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sx3\|3sx3]], [[3sx5\|3sx5]], [[3sx7\|3sx7]], [[3sx8\|3sx8]], [[3sxb\|3sxb]], [[3sxc\|3sxc]], [[3sxd\|3sxd]], [[3sxe\|3sxe]], [[3sxg\|3sxg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sx3\|3sx3]], [[3sx5\|3sx5]], [[3sx7\|3sx7]], [[3sx8\|3sx8]], [[3sxb\|3sxb]], [[3sxc\|3sxc]], [[3sxd\|3sxd]], [[3sxe\|3sxe]], [[3sxg\|3sxg]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sxa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sxa RCSB], [http://www.ebi.ac.uk/pdbsum/3sxa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sxa OCA], [http://pdbe.org/3sxa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3sxa RCSB], [http://www.ebi.ac.uk/pdbsum/3sxa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3sxa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3sxa" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]		[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Evans, S V]]		[[Category: Evans, S V]]
	[[Category: Johal, A R]]		[[Category: Johal, A R]]

OCA at 08:28, 25 December 2014

2014-12-25T08:28:05Z

←Older revision		Revision as of 08:28, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3sxa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SXA FirstGlance]. <br>		<table><tr><td colspan='2'>[[3sxa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SXA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sx3\|3sx3]], [[3sx5\|3sx5]], [[3sx7\|3sx7]], [[3sx8\|3sx8]], [[3sxb\|3sxb]], [[3sxc\|3sxc]], [[3sxd\|3sxd]], [[3sxe\|3sxe]], [[3sxg\|3sxg]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sx3\|3sx3]], [[3sx5\|3sx5]], [[3sx7\|3sx7]], [[3sx8\|3sx8]], [[3sxb\|3sxb]], [[3sxc\|3sxc]], [[3sxd\|3sxd]], [[3sxe\|3sxe]], [[3sxg\|3sxg]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sxa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sxa RCSB], [http://www.ebi.ac.uk/pdbsum/3sxa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sxa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sxa RCSB], [http://www.ebi.ac.uk/pdbsum/3sxa PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BGAT_HUMAN BGAT_HUMAN]] This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	Sequence-dependent effects of cryoprotectants on the active sites of the human ABO(H) blood group A and B glycosyltransferases.,Johal AR, Schuman B, Alfaro JA, Borisova S, Seto NO, Evans SV Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):268-76. Epub 2012 Feb 14. PMID:22349229<ref>PMID:22349229</ref>		Sequence-dependent effects of cryoprotectants on the active sites of the human ABO(H) blood group A and B glycosyltransferases.,Johal AR, Schuman B, Alfaro JA, Borisova S, Seto NO, Evans SV Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):268-76. Epub 2012 Feb 14. PMID:22349229<ref>PMID:22349229</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 23:		Line 25:
	[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]		[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Evans, S V.]]	+	[[Category: Evans, S V]]
-	[[Category: Johal, A R.]]	+	[[Category: Johal, A R]]
	[[Category: Abo rossmann fold]]		[[Category: Abo rossmann fold]]
	[[Category: Blood group antigen]]		[[Category: Blood group antigen]]

OCA at 05:27, 5 June 2014

2014-06-05T05:27:01Z

←Older revision		Revision as of 05:27, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3sxa.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant==
		+	<StructureSection load='3sxa' size='340' side='right' caption='[[3sxa]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3sxa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SXA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sx3\|3sx3]], [[3sx5\|3sx5]], [[3sx7\|3sx7]], [[3sx8\|3sx8]], [[3sxb\|3sxb]], [[3sxc\|3sxc]], [[3sxd\|3sxd]], [[3sxe\|3sxe]], [[3sxg\|3sxg]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sxa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sxa RCSB], [http://www.ebi.ac.uk/pdbsum/3sxa PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The human ABO(H) A and B blood group glycosyltransferases GTA and GTB differ by only four amino acids, yet this small dissimilarity is responsible for significant differences in biosynthesis, kinetics and structure. Like other glycosyltransferases, these two enzymes have been shown to recognize substrates through dramatic conformational changes in mobile polypeptide loops surrounding the active site. Structures of GTA, GTB and several chimeras determined by single-crystal X-ray diffraction demonstrate a range of susceptibility to the choice of cryoprotectant, in which the mobile polypeptide loops can be induced by glycerol to form the ordered closed conformation associated with substrate recognition and by MPD [hexylene glycol, (+/-)-2-methyl-2,4-pentanediol] to hinder binding of substrate in the active site owing to chelation of the Mn(2+) cofactor and thereby adopt the disordered open state. Glycerol is often avoided as a cryoprotectant when determining the structures of carbohydrate-active enzymes as it may act as a competitive inhibitor for monosaccharide ligands. Here, it is shown that the use of glycerol as a cryoprotectant can additionally induce significant changes in secondary structure, a phenomenon that could apply to any class of protein.

-	<!--	+	Sequence-dependent effects of cryoprotectants on the active sites of the human ABO(H) blood group A and B glycosyltransferases.,Johal AR, Schuman B, Alfaro JA, Borisova S, Seto NO, Evans SV Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):268-76. Epub 2012 Feb 14. PMID:22349229<ref>PMID:22349229</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3sxa", creates the "Structure Box"~~ on the ~~page.~~	+
-	~~You may change~~ the ~~PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter~~ (~~which sets the initial scene displayed when the page is loaded~~),	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3sxa\| PDB=3sxa \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~ABBB+UDP+Gal with Glycerol as~~ the ~~cryoprotectant===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22349229}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22349229 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22349229}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3sxa]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022349229<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]		[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 06:53, 28 March 2012

2012-03-28T06:53:58Z

←Older revision		Revision as of 06:53, 28 March 2012
Line 1:		Line 1:
-	[[Image:3sxa.~~jpg~~\|left\|200px]]	+	[[Image:3sxa.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant===		===Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22349229}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22349229 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22349229}}

	==About this Structure==		==About this Structure==
	[[3sxa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA].		[[3sxa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022349229</ref><references group="xtra"/>
	[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]		[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 08:58, 29 February 2012

2012-02-29T08:58:48Z

←Older revision		Revision as of 08:58, 29 February 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3sxa.jpg\|left\|200px]]

-	The ~~entry 3sxa~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3sxa", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3sxa\| PDB=3sxa \| SCENE= }}

-	~~Authors: Johal, A.R., Evans, S.V.~~	+	===Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant===

-	~~Description: Crystal~~ structure ~~of ABBB+UDP+Gal~~ with ~~Glycerol as the cryoprotectant~~	+
		+	==About this Structure==
		+	[[3sxa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXA OCA].
		+	[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Evans, S V.]]
		+	[[Category: Johal, A R.]]
		+	[[Category: Abo rossmann fold]]
		+	[[Category: Blood group antigen]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Manganese]]
		+	[[Category: Metal-binding]]
		+	[[Category: Retaining glycosyltransferase]]
		+	[[Category: Transferase]]

OCA at 05:51, 17 August 2011

2011-08-17T05:51:29Z

←Older revision		Revision as of 05:51, 17 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3sxa is ON HOLD	+	The entry 3sxa is ON HOLD until Paper Publication

	Authors: Johal, A.R., Evans, S.V.		Authors: Johal, A.R., Evans, S.V.

	Description: Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant		Description: Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant

OCA: Protected "3sxa" [edit=sysop:move=sysop]

2011-07-20T06:20:11Z

Protected "3sxa" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:20, 20 July 2011

OCA: New page: '''Unreleased structure''' The entry 3sxa is ON HOLD Authors: Johal, A.R., Evans, S.V. Description: Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant

2011-07-20T06:20:10Z

New page: '''Unreleased structure''' The entry 3sxa is ON HOLD Authors: Johal, A.R., Evans, S.V. Description: Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant

New page

'''Unreleased structure'''

The entry 3sxa is ON HOLD

Authors: Johal, A.R., Evans, S.V.

Description: Crystal structure of ABBB+UDP+Gal with Glycerol as the cryoprotectant