3t90 - Revision history

OCA at 13:21, 14 March 2024

2024-03-14T13:21:10Z

←Older revision		Revision as of 13:21, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3t90' size='340' side='right'caption='[[3t90]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='3t90' size='340' side='right'caption='[[3t90]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t90]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T90 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t90]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T90 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F14F8_150, GNA1, At5g15770, AT5G15770 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t90 OCA], [https://pdbe.org/3t90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t90 RCSB], [https://www.ebi.ac.uk/pdbsum/3t90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t90 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t90 OCA], [https://pdbe.org/3t90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t90 RCSB], [https://www.ebi.ac.uk/pdbsum/3t90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t90 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GNA1_ARATH GNA1_ARATH]] Acetyltransferase involved in UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis. UDP-GlcNAc is an essential metabolite that serves as an initial sugar donor for N-glycan synthesis and thus plays an important role in protein and lipid glycosylation.<ref>PMID:22329777</ref> <ref>PMID:22932674</ref>	+	[https://www.uniprot.org/uniprot/GNA1_ARATH GNA1_ARATH] Acetyltransferase involved in UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis. UDP-GlcNAc is an essential metabolite that serves as an initial sugar donor for N-glycan synthesis and thus plays an important role in protein and lipid glycosylation.<ref>PMID:22329777</ref> <ref>PMID:22932674</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	GlcNAc (N-acetylglucosamine) is an essential part of the glycan chain in N-linked glycoproteins. It is a building block for polysaccharides such as chitin, and several glucosaminoglycans and proteins can be O-GlcNAcylated. The deacetylated form, glucosamine, is an integral part of GPI (glycosylphosphatidylinositol) anchors. Both are incorporated into polymers by glycosyltransferases that utilize UDP-GlcNAc. This UDP-sugar is synthesized in a short pathway comprising four steps starting from fructose 6-phosphate. GNA (glucosamine-6-phosphate N-acetyltransferase) catalyses the second of these four reactions in the de novo synthesis in eukaryotes. A phylogenetic analysis revealed that only one GNA isoform can be found in most of the species investigated and that the most likely Arabidopsis candidate is encoded by the gene At5g15770 (AtGNA). qPCR (quantitative PCR) revealed the ubiquitous expression of AtGNA in all organs of Arabidopsis plants. Heterologous expression of AtGNA showed that it is highly active between pH 7 and 8 and at temperatures of 30-40 degrees C. It showed Km values of 231 muM for glucosamine 6-phosphate and 33 muM for acetyl-CoA respectively and a catalytic efficiency comparable with that of other GNAs characterized. The solved crystal structure of AtGNA at a resolution of 1.5 A (1 A=0.1 nm) revealed a very high structural similarity to crystallized GNA proteins from Homo sapiens and Saccharomyces cerevisiae despite less well conserved protein sequence identity.	+
-		+
-	Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana.,Riegler H, Herter T, Grishkovskaya I, Lude A, Ryngajllo M, Bolger ME, Essigmann B, Usadel B Biochem J. 2012 Apr 15;443(2):427-37. PMID:22329777<ref>PMID:22329777</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3t90" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Grishkovskaya, I]]	+	[[Category: Grishkovskaya I]]
-	[[Category: Herter, T]]	+	[[Category: Herter T]]
-	[[Category: Riegler, H]]	+	[[Category: Riegler H]]
-	[[Category: Usadel, B]]	+	[[Category: Usadel B]]
-	~~[[Category: Acetyltransferase]]~~	+
-	~~[[Category: Alpha/beta protein]]~~	+
-	~~[[Category: Glcnac biosynthesis]]~~	+
-	~~[[Category: Gnat fold]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 16:48, 6 July 2022

2022-07-06T16:48:02Z

←Older revision		Revision as of 16:48, 6 July 2022
Line 1:		Line 1:

	==Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana==		==Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana==
-	<StructureSection load='3t90' size='340' side='right' caption='[[3t90]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='3t90' size='340' side='right'caption='[[3t90]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t90]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T90 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t90]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T90 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F14F8_150, GNA1, At5g15770, AT5G15770 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F14F8_150, GNA1, At5g15770, AT5G15770 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t90 OCA], [~~http~~://pdbe.org/3t90 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t90 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t90 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t90 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t90 OCA], [https://pdbe.org/3t90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t90 RCSB], [https://www.ebi.ac.uk/pdbsum/3t90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t90 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GNA1_ARATH GNA1_ARATH]] Acetyltransferase involved in UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis. UDP-GlcNAc is an essential metabolite that serves as an initial sugar donor for N-glycan synthesis and thus plays an important role in protein and lipid glycosylation.<ref>PMID:22329777</ref> <ref>PMID:22932674</ref>	+	[[https://www.uniprot.org/uniprot/GNA1_ARATH GNA1_ARATH]] Acetyltransferase involved in UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis. UDP-GlcNAc is an essential metabolite that serves as an initial sugar donor for N-glycan synthesis and thus plays an important role in protein and lipid glycosylation.<ref>PMID:22329777</ref> <ref>PMID:22932674</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Arath]]		[[Category: Arath]]
		+	[[Category: Large Structures]]
	[[Category: Grishkovskaya, I]]		[[Category: Grishkovskaya, I]]
	[[Category: Herter, T]]		[[Category: Herter, T]]

OCA at 10:01, 4 August 2016

2016-08-04T10:01:47Z

←Older revision		Revision as of 10:01, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana==		==Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana==
	<StructureSection load='3t90' size='340' side='right' caption='[[3t90]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='3t90' size='340' side='right' caption='[[3t90]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t90]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T90 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t90]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T90 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F14F8_150, GNA1, At5g15770, AT5G15770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F14F8_150, GNA1, At5g15770, AT5G15770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t90 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t90 RCSB], [http://www.ebi.ac.uk/pdbsum/3t90 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t90 OCA], [http://pdbe.org/3t90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t90 RCSB], [http://www.ebi.ac.uk/pdbsum/3t90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t90 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q9LFU9_ARATH Q9LFU9_ARATH~~]] Acetyltransferase involved in UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis. UDP-GlcNAc is an essential metabolite that serves as an initial sugar donor for N-glycan synthesis and thus plays an important role in protein and lipid glycosylation.<ref>PMID:~~22932674~~</ref> <ref>PMID:~~22329777~~</ref>	+	[[http://www.uniprot.org/uniprot/GNA1_ARATH GNA1_ARATH]] Acetyltransferase involved in UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis. UDP-GlcNAc is an essential metabolite that serves as an initial sugar donor for N-glycan synthesis and thus plays an important role in protein and lipid glycosylation.<ref>PMID:22329777</ref> <ref>PMID:22932674</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t90" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabidopsis thaliana~~]]	+	[[Category: Arath]]
	[[Category: Grishkovskaya, I]]		[[Category: Grishkovskaya, I]]
	[[Category: Herter, T]]		[[Category: Herter, T]]

OCA at 08:07, 25 December 2014

2014-12-25T08:07:39Z

←Older revision		Revision as of 08:07, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t90]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T90 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t90]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T90 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F14F8_150, GNA1, At5g15770, AT5G15770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F14F8_150, GNA1, At5g15770, AT5G15770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t90 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t90 RCSB], [http://www.ebi.ac.uk/pdbsum/3t90 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t90 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t90 RCSB], [http://www.ebi.ac.uk/pdbsum/3t90 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q9LFU9_ARATH Q9LFU9_ARATH]] Acetyltransferase involved in UDP-N-acetylglucosamine (UDP-GlcNAc) biosynthesis. UDP-GlcNAc is an essential metabolite that serves as an initial sugar donor for N-glycan synthesis and thus plays an important role in protein and lipid glycosylation.<ref>PMID:22932674</ref> <ref>PMID:22329777</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana.,Riegler H, Herter T, Grishkovskaya I, Lude A, Ryngajllo M, Bolger ME, Essigmann B, Usadel B Biochem J. 2012 Apr 15;443(2):427-37. PMID:22329777<ref>PMID:22329777</ref>		Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana.,Riegler H, Herter T, Grishkovskaya I, Lude A, Ryngajllo M, Bolger ME, Essigmann B, Usadel B Biochem J. 2012 Apr 15;443(2):427-37. PMID:22329777<ref>PMID:22329777</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
-	[[Category: Grishkovskaya, I.]]	+	[[Category: Grishkovskaya, I]]
-	[[Category: Herter, T.]]	+	[[Category: Herter, T]]
-	[[Category: Riegler, H.]]	+	[[Category: Riegler, H]]
-	[[Category: Usadel, B.]]	+	[[Category: Usadel, B]]
	[[Category: Acetyltransferase]]		[[Category: Acetyltransferase]]
	[[Category: Alpha/beta protein]]		[[Category: Alpha/beta protein]]

OCA at 05:59, 5 June 2014

2014-06-05T05:59:37Z

←Older revision		Revision as of 05:59, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3t90.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana==
		+	<StructureSection load='3t90' size='340' side='right' caption='[[3t90]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t90]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T90 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">F14F8_150, GNA1, At5g15770, AT5G15770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t90 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t90 RCSB], [http://www.ebi.ac.uk/pdbsum/3t90 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GlcNAc (N-acetylglucosamine) is an essential part of the glycan chain in N-linked glycoproteins. It is a building block for polysaccharides such as chitin, and several glucosaminoglycans and proteins can be O-GlcNAcylated. The deacetylated form, glucosamine, is an integral part of GPI (glycosylphosphatidylinositol) anchors. Both are incorporated into polymers by glycosyltransferases that utilize UDP-GlcNAc. This UDP-sugar is synthesized in a short pathway comprising four steps starting from fructose 6-phosphate. GNA (glucosamine-6-phosphate N-acetyltransferase) catalyses the second of these four reactions in the de novo synthesis in eukaryotes. A phylogenetic analysis revealed that only one GNA isoform can be found in most of the species investigated and that the most likely Arabidopsis candidate is encoded by the gene At5g15770 (AtGNA). qPCR (quantitative PCR) revealed the ubiquitous expression of AtGNA in all organs of Arabidopsis plants. Heterologous expression of AtGNA showed that it is highly active between pH 7 and 8 and at temperatures of 30-40 degrees C. It showed Km values of 231 muM for glucosamine 6-phosphate and 33 muM for acetyl-CoA respectively and a catalytic efficiency comparable with that of other GNAs characterized. The solved crystal structure of AtGNA at a resolution of 1.5 A (1 A=0.1 nm) revealed a very high structural similarity to crystallized GNA proteins from Homo sapiens and Saccharomyces cerevisiae despite less well conserved protein sequence identity.

-	<!--	+	Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana.,Riegler H, Herter T, Grishkovskaya I, Lude A, Ryngajllo M, Bolger ME, Essigmann B, Usadel B Biochem J. 2012 Apr 15;443(2):427-37. PMID:22329777<ref>PMID:22329777</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3t90"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3t90\| PDB=3t90 \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22329777}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22329777 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22329777}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3t90]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022329777<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Grishkovskaya, I.]]		[[Category: Grishkovskaya, I.]]

OCA at 07:02, 11 April 2012

2012-04-11T07:02:21Z

←Older revision		Revision as of 07:02, 11 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t90.jpg\|left\|200px]]

-	The ~~entry 3t90~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t90", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t90\| PDB=3t90 \| SCENE= }}

-	~~Authors: Grishkovskaya, I., Herter, T., Riegler, H., Usadel, B.~~	+	===Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana===

-	~~Description: Crystal structure of glucosamine~~-6-~~phosphate N~~-~~acetyltransferase~~ from Arabidopsis thaliana	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22329777}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22329777 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22329777}}
		+
		+	==About this Structure==
		+	[[3t90]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T90 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022329777</ref><references group="xtra"/>
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Grishkovskaya, I.]]
		+	[[Category: Herter, T.]]
		+	[[Category: Riegler, H.]]
		+	[[Category: Usadel, B.]]
		+	[[Category: Acetyltransferase]]
		+	[[Category: Alpha/beta protein]]
		+	[[Category: Glcnac biosynthesis]]
		+	[[Category: Gnat fold]]
		+	[[Category: Transferase]]

OCA at 07:09, 24 August 2011

2011-08-24T07:09:23Z

←Older revision		Revision as of 07:09, 24 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t90 is ON HOLD	+	The entry 3t90 is ON HOLD until Paper Publication

	Authors: Grishkovskaya, I., Herter, T., Riegler, H., Usadel, B.		Authors: Grishkovskaya, I., Herter, T., Riegler, H., Usadel, B.

	Description: Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana		Description: Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana

OCA at 05:55, 17 August 2011

2011-08-17T05:55:02Z

←Older revision		Revision as of 05:55, 17 August 2011
Line 3:		Line 3:
	The entry 3t90 is ON HOLD		The entry 3t90 is ON HOLD

-	Authors: Grishkovskaya I., Herter T., Riegler H., Usadel B.	+	Authors: Grishkovskaya, I., Herter, T., Riegler, H., Usadel, B.

	Description: Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana		Description: Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana

OCA: Protected "3t90" [edit=sysop:move=sysop]

2011-08-10T05:37:39Z

Protected "3t90" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:37, 10 August 2011

OCA: New page: '''Unreleased structure''' The entry 3t90 is ON HOLD Authors: Grishkovskaya I., Herter T., Riegler H., Usadel B. Description: Crystal structure of glucosamine-6-phosphate N-acetyltrans...

2011-08-10T05:37:38Z

New page: '''Unreleased structure''' The entry 3t90 is ON HOLD Authors: Grishkovskaya I., Herter T., Riegler H., Usadel B. Description: Crystal structure of glucosamine-6-phosphate N-acetyltrans...

New page

'''Unreleased structure'''

The entry 3t90 is ON HOLD

Authors: Grishkovskaya I., Herter T., Riegler H., Usadel B.

Description: Crystal structure of glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana