3tbc - Revision history

OCA at 10:07, 1 March 2024

2024-03-01T10:07:25Z

←Older revision		Revision as of 10:07, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3tbc' size='340' side='right'caption='[[3tbc]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3tbc' size='340' side='right'caption='[[3tbc]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tbc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_27479 Atcc 27479~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TBC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tbc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_viridosporus Streptomyces viridosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TBC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=I75:1-(4-HYDROXY-3-METHOXYPHENYL)ETHANONE'>I75</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tbb\|3tbb]], [[3tas\|3tas]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=I75:1-(4-HYDROXY-3-METHOXYPHENYL)ETHANONE'>I75</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Laccase Laccase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbc OCA], [https://pdbe.org/3tbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tbc RCSB], [https://www.ebi.ac.uk/pdbsum/3tbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tbc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbc OCA], [https://pdbe.org/3tbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tbc RCSB], [https://www.ebi.ac.uk/pdbsum/3tbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tbc ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/J9PBR2_STRVD J9PBR2_STRVD]
-	Laccases (EC 1.10.3.2) are multicopper oxidases able to oxidize a range of substrates including phenols, aromatic amines and non-phenolic substrates. In order to investigate the involvement of the small Streptomyces laccases in lignin degradation, we generated acid-precipitable polymeric lignin (APPL) obtained in the presence of wild type Streptomyces coelicolor A3(2) (SCWT) and its laccase-less mutant (SCDeltaLAC) in presence of Miscanthus x giganteus lignocellulose. The results showed that strain SCDeltaLAC was inefficient in degrading lignin compared to strain SCWT, thereby supporting the importance of laccase for lignin degradation by Streptomyces coelicolor A3(2). We also studied the lignin degradation activity of laccases from Streptomyces coelicolor A3(2), Streptomyces lividans TK24, Streptomyces viridosporus T7A and Amycolatopsis sp. 75iv2 using both lignin model compounds and ethanosolv lignin. All four laccases degraded a phenolic model compound (LM-OH), but only able to oxidize a non-phenolic model compound (LM-OMe) in the presence of redox mediators. Their activities are highest at pH 8.0 with a low krel/Kapp for LM-OH, suggesting that the enzymes' natural substrates must be different in shape or chemical nature. Crystal structures of the laccases from Streptomyces viridosporus T7A (SVLAC) and Amycolatopsis sp. 75iv2 (AMLAC) were determined both with and without bound substrate. This is the first report of a crystal structure for any laccase bound to a non-phenolic beta-O-4 lignin model compound. An additional zinc metal binding site in SVLAC was also identified. The ability to oxidize/rearrange ethanosolv lignin further provides evidence for the utility of laccase activity for lignin degradation/modification.	+
-		+
-	~~The roles of small laccases from Streptomyces in lignin degradation.,Majumdar S, Lukk T, Solbiati JO, Bauer S, Nair SK, Cronan JE, Gerlt JA Biochemistry. 2014 May 28. PMID~~:~~24870309<ref>PMID:24870309<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3tbc" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Laccase 3D structures\|Laccase 3D structures]]		*[[Laccase 3D structures\|Laccase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 27479]]
-	[[Category: Laccase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Gerlt, J A~~]]	+	[[Category: Streptomyces viridosporus]]
-	[[Category: ~~Lukk, T~~]]	+	[[Category: Gerlt JA]]
-	[[Category: ~~Majumdar, S~~]]	+	[[Category: Lukk T]]
-	[[Category: ~~Nair,~~ S K]]	+	[[Category: Majumdar S]]
-	[[Category: ~~Acitovanillone]]~~	+	[[Category: Nair SK]]
-	~~[[Category: None]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Two-domain laccase~~]]	+

OCA at 16:50, 6 July 2022

2022-07-06T16:50:56Z

←Older revision		Revision as of 16:50, 6 July 2022
Line 3:		Line 3:
	<StructureSection load='3tbc' size='340' side='right'caption='[[3tbc]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3tbc' size='340' side='right'caption='[[3tbc]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tbc]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_27479 Atcc 27479]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TBC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tbc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_27479 Atcc 27479]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TBC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=I75:1-(4-HYDROXY-3-METHOXYPHENYL)ETHANONE'>I75</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=I75:1-(4-HYDROXY-3-METHOXYPHENYL)ETHANONE'>I75</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tbb\|3tbb]], [[3tas\|3tas]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tbb\|3tbb]], [[3tas\|3tas]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Laccase Laccase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Laccase Laccase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbc OCA], [~~http~~://pdbe.org/3tbc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tbc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tbc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tbc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbc OCA], [https://pdbe.org/3tbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tbc RCSB], [https://www.ebi.ac.uk/pdbsum/3tbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tbc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:44, 7 August 2019

2019-08-07T06:44:19Z

←Older revision		Revision as of 06:44, 7 August 2019
Line 1:		Line 1:

	==Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.==		==Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.==
-	<StructureSection load='3tbc' size='340' side='right' caption='[[3tbc]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='3tbc' size='340' side='right'caption='[[3tbc]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tbc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27479 Atcc 27479]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TBC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tbc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27479 Atcc 27479]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TBC FirstGlance]. <br>
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 3tbc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3tbc" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Laccase 3D structures\|Laccase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	[[Category: Atcc 27479]]		[[Category: Atcc 27479]]
	[[Category: Laccase]]		[[Category: Laccase]]
		+	[[Category: Large Structures]]
	[[Category: Gerlt, J A]]		[[Category: Gerlt, J A]]
	[[Category: Lukk, T]]		[[Category: Lukk, T]]

OCA at 17:50, 11 August 2016

2016-08-11T17:50:01Z

←Older revision		Revision as of 17:50, 11 August 2016
Line 1:		Line 1:
		+
	==Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.==		==Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.==
	<StructureSection load='3tbc' size='340' side='right' caption='[[3tbc]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3tbc' size='340' side='right' caption='[[3tbc]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tbb\|3tbb]], [[3tas\|3tas]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tbb\|3tbb]], [[3tas\|3tas]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tbc RCSB], [http://www.ebi.ac.uk/pdbsum/3tbc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbc OCA], [http://pdbe.org/3tbc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tbc RCSB], [http://www.ebi.ac.uk/pdbsum/3tbc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tbc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tbc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:50, 20 January 2015

2015-01-20T07:50:43Z

←Older revision		Revision as of 07:50, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tbc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27479 Atcc 27479]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TBC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tbc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27479 Atcc 27479]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TBC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=I75:1-(4-HYDROXY-3-METHOXYPHENYL)ETHANONE'>I75</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=I75:1-(4-HYDROXY-3-METHOXYPHENYL)ETHANONE'>I75</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tbb\|3tbb]], [[3tas\|3tas]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tbb\|3tbb]], [[3tas\|3tas]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tbc RCSB], [http://www.ebi.ac.uk/pdbsum/3tbc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tbc RCSB], [http://www.ebi.ac.uk/pdbsum/3tbc PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	[[Category: Atcc 27479]]		[[Category: Atcc 27479]]
	[[Category: Laccase]]		[[Category: Laccase]]
-	[[Category: Gerlt, J A.]]	+	[[Category: Gerlt, J A]]
-	[[Category: Lukk, T.]]	+	[[Category: Lukk, T]]
-	[[Category: Majumdar, S.]]	+	[[Category: Majumdar, S]]
-	[[Category: Nair, S K.]]	+	[[Category: Nair, S K]]
	[[Category: Acitovanillone]]		[[Category: Acitovanillone]]
	[[Category: None]]		[[Category: None]]

OCA at 06:46, 11 June 2014

2014-06-11T06:46:50Z

←Older revision		Revision as of 06:46, 11 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3tbc.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.==
		+	<StructureSection load='3tbc' size='340' side='right' caption='[[3tbc]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tbc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27479 Atcc 27479]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TBC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=I75:1-(4-HYDROXY-3-METHOXYPHENYL)ETHANONE'>I75</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tbb\|3tbb]], [[3tas\|3tas]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tbc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tbc RCSB], [http://www.ebi.ac.uk/pdbsum/3tbc PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Laccases (EC 1.10.3.2) are multicopper oxidases able to oxidize a range of substrates including phenols, aromatic amines and non-phenolic substrates. In order to investigate the involvement of the small Streptomyces laccases in lignin degradation, we generated acid-precipitable polymeric lignin (APPL) obtained in the presence of wild type Streptomyces coelicolor A3(2) (SCWT) and its laccase-less mutant (SCDeltaLAC) in presence of Miscanthus x giganteus lignocellulose. The results showed that strain SCDeltaLAC was inefficient in degrading lignin compared to strain SCWT, thereby supporting the importance of laccase for lignin degradation by Streptomyces coelicolor A3(2). We also studied the lignin degradation activity of laccases from Streptomyces coelicolor A3(2), Streptomyces lividans TK24, Streptomyces viridosporus T7A and Amycolatopsis sp. 75iv2 using both lignin model compounds and ethanosolv lignin. All four laccases degraded a phenolic model compound (LM-OH), but only able to oxidize a non-phenolic model compound (LM-OMe) in the presence of redox mediators. Their activities are highest at pH 8.0 with a low krel/Kapp for LM-OH, suggesting that the enzymes' natural substrates must be different in shape or chemical nature. Crystal structures of the laccases from Streptomyces viridosporus T7A (SVLAC) and Amycolatopsis sp. 75iv2 (AMLAC) were determined both with and without bound substrate. This is the first report of a crystal structure for any laccase bound to a non-phenolic beta-O-4 lignin model compound. An additional zinc metal binding site in SVLAC was also identified. The ability to oxidize/rearrange ethanosolv lignin further provides evidence for the utility of laccase activity for lignin degradation/modification.

-	~~{{STRUCTURE_3tbc\| PDB=3tbc \| SCENE= }}~~	+	The roles of small laccases from Streptomyces in lignin degradation.,Majumdar S, Lukk T, Solbiati JO, Bauer S, Nair SK, Cronan JE, Gerlt JA Biochemistry. 2014 May 28. PMID:24870309<ref>PMID:24870309</ref>

-	~~===Small laccase from Streptomyces viridosporus T7A~~; ~~alternate crystal form complexed with acetovanillone~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	==~~About this Structure~~==	+	<references/>
-	~~[[3tbc]] is a 3 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Streptomyces_viridosporus Streptomyces viridosporus]. Full crystallographic information is available from~~ [~~http~~:~~//oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA~~].	+	__TOC__
		+	</StructureSection>
		+	[[Category: Atcc 27479]]
	[[Category: Laccase]]		[[Category: Laccase]]
-	[[Category: Streptomyces viridosporus]]
	[[Category: Gerlt, J A.]]		[[Category: Gerlt, J A.]]
	[[Category: Lukk, T.]]		[[Category: Lukk, T.]]

OCA at 05:17, 29 August 2012

2012-08-29T05:17:05Z

←Older revision		Revision as of 05:17, 29 August 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tbc.jpg\|left\|200px]]

-	~~The entry~~ 3tbc ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3tbc\| PDB=3tbc \| SCENE= }}

-	~~Authors: Lukk, T., Majumdar, S., Gerlt, J.A., Nair, S.K~~.	+	===Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.===

-	~~Description~~: ~~Small laccase~~ from Streptomyces viridosporus ~~T7A; alternate crystal form complexed with acetovanillone~~.	+
		+	==About this Structure==
		+	[[3tbc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_viridosporus Streptomyces viridosporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TBC OCA].
		+	[[Category: Laccase]]
		+	[[Category: Streptomyces viridosporus]]
		+	[[Category: Gerlt, J A.]]
		+	[[Category: Lukk, T.]]
		+	[[Category: Majumdar, S.]]
		+	[[Category: Nair, S K.]]
		+	[[Category: Acitovanillone]]
		+	[[Category: None]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Secreted]]
		+	[[Category: Two-domain laccase]]

OCA at 07:10, 24 August 2011

2011-08-24T07:10:01Z

←Older revision		Revision as of 07:10, 24 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3tbc is ON HOLD	+	The entry 3tbc is ON HOLD until Paper Publication

	Authors: Lukk, T., Majumdar, S., Gerlt, J.A., Nair, S.K.		Authors: Lukk, T., Majumdar, S., Gerlt, J.A., Nair, S.K.

	Description: Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.		Description: Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.

OCA: Protected "3tbc" [edit=sysop:move=sysop]

2011-08-17T05:55:53Z

Protected "3tbc" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:55, 17 August 2011

OCA: New page: '''Unreleased structure''' The entry 3tbc is ON HOLD Authors: Lukk, T., Majumdar, S., Gerlt, J.A., Nair, S.K. Description: Small laccase from Streptomyces viridosporus T7A; alternate c...

2011-08-17T05:55:52Z

New page: '''Unreleased structure''' The entry 3tbc is ON HOLD Authors: Lukk, T., Majumdar, S., Gerlt, J.A., Nair, S.K. Description: Small laccase from Streptomyces viridosporus T7A; alternate c...

New page

'''Unreleased structure'''

The entry 3tbc is ON HOLD

Authors: Lukk, T., Majumdar, S., Gerlt, J.A., Nair, S.K.

Description: Small laccase from Streptomyces viridosporus T7A; alternate crystal form complexed with acetovanillone.