3tnf - Revision history

OCA at 10:10, 1 March 2024

2024-03-01T10:10:18Z

←Older revision		Revision as of 10:10, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3tnf' size='340' side='right'caption='[[3tnf]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3tnf' size='340' side='right'caption='[[3tnf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~] and [https://en.wikipedia.org/wiki/~~Legph Legph~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TNF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TNF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qbt\|3qbt]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MEL, RAB8, RAB8A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), lida, lpg0940 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [https://pdbe.org/3tnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [https://www.ebi.ac.uk/pdbsum/3tnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tnf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [https://pdbe.org/3tnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [https://www.ebi.ac.uk/pdbsum/3tnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tnf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN]] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>	+	[https://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The causative agent of Legionnaires disease, Legionella pneumophila, injects several hundred proteins into the cell it infects, many of which interfere with or exploit vesicular transport processes. One of these proteins, LidA, has been described as a Rab effector (i.e., a molecule that interacts preferentially with the GTP-bound form of Rab). We describe here the structure and biochemistry of a complex between the Rab-binding domain of LidA and active Rab8a. LidA displays structural peculiarities in binding to Rab8a, forming a considerably extended interface in comparison to known mammalian Rab effectors, and involving regions of the GTPase that are not seen in other Rab:effector complexes. In keeping with this extended binding interface, which involves four alpha-helices and two pillar-like structures of LidA, the stability of LidA-Rab interactions is dramatically greater than for other such complexes. For Rab1b and Rab8a, these affinities are extraordinarily high, but for the more weakly bound Rab6a, K(d) values of 4 nM for the inactive and 30 pM for the active form were found. Rab1b and Rab8a appear to bind LidA with K(d) values in the low picomolar range, making LidA a Rab supereffector.	+

-	~~Protein LidA from Legionella is a Rab GTPase supereffector.,Schoebel S, Cichy AL, Goody RS, Itzen A Proc Natl Acad Sci U S A. 2011 Oct 19. PMID:22011575<ref>PMID:22011575</ref>~~	+	==See Also==
-		+	*[[Ras-related protein Rab 3D structures\|Ras-related protein Rab 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 3tnf" style~~=~~"background~~-~~color:#fffaf0;"></div>~~	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Legph~~]]	+	[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
-	[[Category: Cichy~~, A L~~]]	+	[[Category: Cichy AL]]
-	[[Category: Goody~~, R S~~]]	+	[[Category: Goody RS]]
-	[[Category: Itzen, A]]	+	[[Category: Itzen A]]
-	[[Category: Schoebel, S]]	+	[[Category: Schoebel S]]
-	~~[[Category: Drra]]~~	+
-	~~[[Category: Er]]~~	+
-	~~[[Category: Gdp/gtp binding rab-binding]]~~	+
-	~~[[Category: Golgi]]~~	+
-	~~[[Category: Gtpase]]~~	+
-	~~[[Category: Lcv]]~~	+
-	~~[[Category: Legionella pneumophila]]~~	+
-	~~[[Category: Plasmamembrane]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Rab-effector]]~~	+
-	~~[[Category: Rab8a]]~~	+
-	~~[[Category: Sidm]]~~	+
-	~~[[Category: Vesicle recuitment]]~~	+
-	~~[[Category: Vesicular trafficking]]~~	+
-	~~[[Category: Vesicular transport~~]]	+

OCA at 17:05, 6 July 2022

2022-07-06T17:05:22Z

←Older revision		Revision as of 17:05, 6 July 2022
Line 1:		Line 1:

	==LidA from Legionella in complex with active Rab8a==		==LidA from Legionella in complex with active Rab8a==
-	<StructureSection load='3tnf' size='340' side='right' caption='[[3tnf]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3tnf' size='340' side='right'caption='[[3tnf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human] and [~~http~~://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TNF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human] and [https://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TNF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qbt\|3qbt]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qbt\|3qbt]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MEL, RAB8, RAB8A ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), lida, lpg0940 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MEL, RAB8, RAB8A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), lida, lpg0940 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [~~http~~://pdbe.org/3tnf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tnf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tnf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [https://pdbe.org/3tnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [https://www.ebi.ac.uk/pdbsum/3tnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tnf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN]] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>	+	[[https://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN]] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Legph]]		[[Category: Legph]]
	[[Category: Cichy, A L]]		[[Category: Cichy, A L]]

OCA at 11:09, 5 August 2016

2016-08-05T11:09:17Z

←Older revision		Revision as of 11:09, 5 August 2016
Line 1:		Line 1:
		+
	==LidA from Legionella in complex with active Rab8a==		==LidA from Legionella in complex with active Rab8a==
	<StructureSection load='3tnf' size='340' side='right' caption='[[3tnf]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3tnf' size='340' side='right' caption='[[3tnf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~] and [http://en.wikipedia.org/wiki/~~Legionella_pneumophila_subsp._pneumophila_str._philadelphia_1 Legionella pneumophila subsp. pneumophila str. philadelphia 1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TNF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TNF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qbt\|3qbt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qbt\|3qbt]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MEL, RAB8, RAB8A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), lida, lpg0940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 ~~Legionella pneumophila subsp. pneumophila str. Philadelphia 1~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MEL, RAB8, RAB8A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), lida, lpg0940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [http://www.ebi.ac.uk/pdbsum/3tnf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [http://pdbe.org/3tnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [http://www.ebi.ac.uk/pdbsum/3tnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tnf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tnf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
-	[[Category: ~~Legionella pneumophila subsp. pneumophila str. philadelphia 1~~]]	+	[[Category: Legph]]
	[[Category: Cichy, A L]]		[[Category: Cichy, A L]]
	[[Category: Goody, R S]]		[[Category: Goody, R S]]

OCA at 01:52, 25 December 2014

2014-12-25T01:52:48Z

←Older revision		Revision as of 01:52, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._philadelphia_1 Legionella pneumophila subsp. pneumophila str. philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TNF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._philadelphia_1 Legionella pneumophila subsp. pneumophila str. philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TNF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qbt\|3qbt]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qbt\|3qbt]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MEL, RAB8, RAB8A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), lida, lpg0940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 Legionella pneumophila subsp. pneumophila str. Philadelphia 1])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MEL, RAB8, RAB8A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), lida, lpg0940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 Legionella pneumophila subsp. pneumophila str. Philadelphia 1])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [http://www.ebi.ac.uk/pdbsum/3tnf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [http://www.ebi.ac.uk/pdbsum/3tnf PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN]] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	Protein LidA from Legionella is a Rab GTPase supereffector.,Schoebel S, Cichy AL, Goody RS, Itzen A Proc Natl Acad Sci U S A. 2011 Oct 19. PMID:22011575<ref>PMID:22011575</ref>		Protein LidA from Legionella is a Rab GTPase supereffector.,Schoebel S, Cichy AL, Goody RS, Itzen A Proc Natl Acad Sci U S A. 2011 Oct 19. PMID:22011575<ref>PMID:22011575</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 24:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Legionella pneumophila subsp. pneumophila str. philadelphia 1]]		[[Category: Legionella pneumophila subsp. pneumophila str. philadelphia 1]]
-	[[Category: Cichy, A L.]]	+	[[Category: Cichy, A L]]
-	[[Category: Goody, R S.]]	+	[[Category: Goody, R S]]
-	[[Category: Itzen, A.]]	+	[[Category: Itzen, A]]
-	[[Category: Schoebel, S.]]	+	[[Category: Schoebel, S]]
	[[Category: Drra]]		[[Category: Drra]]
	[[Category: Er]]		[[Category: Er]]

OCA at 06:15, 5 June 2014

2014-06-05T06:15:47Z

←Older revision		Revision as of 06:15, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3tnf.~~png~~\|~~left~~\|~~200px~~]]	+	==LidA from Legionella in complex with active Rab8a==
		+	<StructureSection load='3tnf' size='340' side='right' caption='[[3tnf]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._philadelphia_1 Legionella pneumophila subsp. pneumophila str. philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TNF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qbt\|3qbt]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MEL, RAB8, RAB8A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), lida, lpg0940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 Legionella pneumophila subsp. pneumophila str. Philadelphia 1])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [http://www.ebi.ac.uk/pdbsum/3tnf PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The causative agent of Legionnaires disease, Legionella pneumophila, injects several hundred proteins into the cell it infects, many of which interfere with or exploit vesicular transport processes. One of these proteins, LidA, has been described as a Rab effector (i.e., a molecule that interacts preferentially with the GTP-bound form of Rab). We describe here the structure and biochemistry of a complex between the Rab-binding domain of LidA and active Rab8a. LidA displays structural peculiarities in binding to Rab8a, forming a considerably extended interface in comparison to known mammalian Rab effectors, and involving regions of the GTPase that are not seen in other Rab:effector complexes. In keeping with this extended binding interface, which involves four alpha-helices and two pillar-like structures of LidA, the stability of LidA-Rab interactions is dramatically greater than for other such complexes. For Rab1b and Rab8a, these affinities are extraordinarily high, but for the more weakly bound Rab6a, K(d) values of 4 nM for the inactive and 30 pM for the active form were found. Rab1b and Rab8a appear to bind LidA with K(d) values in the low picomolar range, making LidA a Rab supereffector.

-	~~<!--~~	+	Protein LidA from Legionella is a Rab GTPase supereffector.,Schoebel S, Cichy AL, Goody RS, Itzen A Proc Natl Acad Sci U S A. 2011 Oct 19. PMID:22011575<ref>PMID:22011575</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3tnf"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3tnf\| PDB=3tnf \| SCENE= }}~~	+

-	~~===LidA from Legionella in complex with active Rab8a===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22011575}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22011575 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22011575}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3tnf]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._philadelphia_1 Legionella pneumophila subsp. pneumophila str. philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022011575<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Legionella pneumophila subsp. pneumophila str. philadelphia 1]]		[[Category: Legionella pneumophila subsp. pneumophila str. philadelphia 1]]

OCA at 18:21, 23 November 2011

2011-11-23T18:21:44Z

←Older revision		Revision as of 18:21, 23 November 2011
Line 1:		Line 1:
-	[[Image:3tnf.~~jpg~~\|left\|200px]]	+	[[Image:3tnf.png\|left\|200px]]

	<!--		<!--

OCA at 08:26, 2 November 2011

2011-11-02T08:26:40Z

←Older revision		Revision as of 08:26, 2 November 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tnf.jpg\|left\|200px]]

-	The ~~entry 3tnf~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tnf", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tnf\| PDB=3tnf \| SCENE= }}

-	~~Authors: Schoebel, S., Cichy, A.L., Goody, R.S., Itzen, A.~~	+	===LidA from Legionella in complex with active Rab8a===

-	~~Description~~: ~~LidA~~ from Legionella ~~in complex with active~~ Rab8a	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22011575}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22011575 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22011575}}
		+
		+	==About this Structure==
		+	[[3tnf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._philadelphia_1 Legionella pneumophila subsp. pneumophila str. philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022011575</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Legionella pneumophila subsp. pneumophila str. philadelphia 1]]
		+	[[Category: Cichy, A L.]]
		+	[[Category: Goody, R S.]]
		+	[[Category: Itzen, A.]]
		+	[[Category: Schoebel, S.]]
		+	[[Category: Drra]]
		+	[[Category: Er]]
		+	[[Category: Gdp/gtp binding rab-binding]]
		+	[[Category: Golgi]]
		+	[[Category: Gtpase]]
		+	[[Category: Lcv]]
		+	[[Category: Legionella pneumophila]]
		+	[[Category: Plasmamembrane]]
		+	[[Category: Protein transport]]
		+	[[Category: Rab-effector]]
		+	[[Category: Rab8a]]
		+	[[Category: Sidm]]
		+	[[Category: Vesicle recuitment]]
		+	[[Category: Vesicular trafficking]]
		+	[[Category: Vesicular transport]]

OCA at 06:05, 14 September 2011

2011-09-14T06:05:34Z

←Older revision		Revision as of 06:05, 14 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3tnf is ON HOLD	+	The entry 3tnf is ON HOLD until Paper Publication

	Authors: Schoebel, S., Cichy, A.L., Goody, R.S., Itzen, A.		Authors: Schoebel, S., Cichy, A.L., Goody, R.S., Itzen, A.

	Description: LidA from Legionella in complex with active Rab8a		Description: LidA from Legionella in complex with active Rab8a

OCA: Protected "3tnf" [edit=sysop:move=sysop]

2011-09-07T05:51:47Z

Protected "3tnf" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:51, 7 September 2011

OCA: New page: '''Unreleased structure''' The entry 3tnf is ON HOLD Authors: Schoebel, S., Cichy, A.L., Goody, R.S., Itzen, A. Description: LidA from Legionella in complex with active Rab8a

2011-09-07T05:51:46Z

New page: '''Unreleased structure''' The entry 3tnf is ON HOLD Authors: Schoebel, S., Cichy, A.L., Goody, R.S., Itzen, A. Description: LidA from Legionella in complex with active Rab8a

New page

'''Unreleased structure'''

The entry 3tnf is ON HOLD

Authors: Schoebel, S., Cichy, A.L., Goody, R.S., Itzen, A.

Description: LidA from Legionella in complex with active Rab8a