3tpf - Revision history

OCA at 13:35, 14 March 2024

2024-03-14T13:35:52Z

←Older revision		Revision as of 13:35, 14 March 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/OTCA_CAMJE OTCA_CAMJE] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase (ArgG) involved in the final step in arginine biosynthesis.<ref>PMID:22949186</ref>		[https://www.uniprot.org/uniprot/OTCA_CAMJE OTCA_CAMJE] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase (ArgG) involved in the final step in arginine biosynthesis.<ref>PMID:22949186</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Anabolic ornithine transcarbamoylase (aOTC) catalyzes the reaction between carbamoyl phosphate (CP) and L-ornithine (ORN) to form L-citrulline and phosphate in the urea cycle and L-arginine biosynthesis. The crystal structure of unliganded aOTC from Campylobacter jejuni (Cje aOTC) was determined at 2.7 A resolution and refined to an R(work) of 20.3% and an R(free) of 24.0%. Cje aOTC is a trimer that forms a head-to-head pseudohexamer in the asymmetric unit. Each monomer is composed of an N-terminal CP-binding domain and a C-terminal ORN-binding domain joined by two interdomain helices. The Cje aOTC structure presents an open conformation of the enzyme with a relatively flexible orientation of the ORN-binding domain respective to the CP-binding domain. The conformation of the B2-H3 loop (residues 68-78), which is involved in binding CP in an adjacent subunit of the trimer, differs from that seen in homologous proteins with CP bound. The loop containing the ORN-binding motif (DxxxSMG, residues 223-230) has a conformation that is different from those observed in unliganded OTC structures from other species, but is similar to those in structures with bound ORN analogs. The major differences in tertiary structure between Cje aOTC and human aOTC are described.
-
-	Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 A resolution.,Shabalin IG, Porebski PJ, Cooper DR, Grabowski M, Onopriyenko O, Grimshaw S, Savchenko A, Chruszcz M, Minor W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep;68(Pt 9):1018-24. Epub, 2012 Aug 29. PMID:22949186<ref>PMID:22949186</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3tpf" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 08:16, 6 December 2023

2023-12-06T08:16:41Z

←Older revision		Revision as of 08:16, 6 December 2023
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	<StructureSection load='3tpf' size='340' side='right'caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3tpf' size='340' side='right'caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Camje Camje~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TPF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_NCTC_11168_=_ATCC_700819 Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TPF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1c9y\|1c9y]]</div></td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c~~ (~~[https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192222 CAMJE]~~)~~</td></tr>~~	+
-	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [https://pdbe.org/3tpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [https://www.ebi.ac.uk/pdbsum/3tpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [https://pdbe.org/3tpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [https://www.ebi.ac.uk/pdbsum/3tpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~OTC_CAMJE OTC_CAMJE]~~] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline (~~By similarity~~).~~[HAMAP-Rule~~:~~MF_01109]~~	+	[https://www.uniprot.org/uniprot/OTCA_CAMJE OTCA_CAMJE] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase (ArgG) involved in the final step in arginine biosynthesis.<ref>PMID:22949186</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camje~~]]	+	[[Category: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Ornithine carbamoyltransferase]]~~	+	[[Category: Anderson WF]]
-	[[Category: Anderson~~, W F]]~~	+	[[Category: Chruszcz M]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Grabowski M]]
-	[[Category: Chruszcz, M]]	+	[[Category: Grimshaw S]]
-	[[Category: Grabowski, M]]	+	[[Category: Minor W]]
-	[[Category: Grimshaw, S]]	+	[[Category: Onopriyenko O]]
-	[[Category: Minor, W]]	+	[[Category: Porebski PJ]]
-	[[Category: Onopriyenko, O]]	+	[[Category: Savchenko A]]
-	[[Category: Porebski~~, P J~~]]	+	[[Category: Shabalin IG]]
-	[[Category: Savchenko, A]]	+
-	[[Category: Shabalin~~, I G]]~~	+
-	~~[[Category: Carbamoyl phosphate binding]]~~	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: L-ornithine binding]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:00, 13 April 2022

2022-04-13T10:00:06Z

←Older revision		Revision as of 10:00, 13 April 2022
Line 1:		Line 1:

	==Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168==		==Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168==
-	<StructureSection load='3tpf' size='340' side='right' caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='3tpf' size='340' side='right'caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Camje Camje]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TPF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Camje Camje]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TPF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c9y\|1c9y]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1c9y\|1c9y]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192222 CAMJE])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192222 CAMJE])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [~~http~~://pdbe.org/3tpf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tpf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [https://pdbe.org/3tpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [https://www.ebi.ac.uk/pdbsum/3tpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/OTC_CAMJE OTC_CAMJE]] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline (By similarity).[HAMAP-Rule:MF_01109]	+	[[https://www.uniprot.org/uniprot/OTC_CAMJE OTC_CAMJE]] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline (By similarity).[HAMAP-Rule:MF_01109]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:

	==See Also==		==See Also==
-	*[[Ornithine carbamoyltransferase\|Ornithine carbamoyltransferase]]	+	*[[Ornithine carbamoyltransferase 3D structures\|Ornithine carbamoyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Camje]]		[[Category: Camje]]
		+	[[Category: Large Structures]]
	[[Category: Ornithine carbamoyltransferase]]		[[Category: Ornithine carbamoyltransferase]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]

OCA at 11:32, 5 August 2016

2016-08-05T11:32:35Z

←Older revision		Revision as of 11:32, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168==		==Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168==
	<StructureSection load='3tpf' size='340' side='right' caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3tpf' size='340' side='right' caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 8:		Line 9:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192222 CAMJE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192222 CAMJE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://pdbe.org/3tpf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tpf" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 02:03, 25 December 2014

2014-12-25T02:03:37Z

←Older revision		Revision as of 02:03, 25 December 2014
Line 2:		Line 2:
	<StructureSection load='3tpf' size='340' side='right' caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3tpf' size='340' side='right' caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Camje Camje]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c9y\|1c9y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c9y\|1c9y]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192222 CAMJE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/OTC_CAMJE OTC_CAMJE]] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline (By similarity).[HAMAP-Rule:MF_01109]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camje]]
	[[Category: Ornithine carbamoyltransferase]]		[[Category: Ornithine carbamoyltransferase]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]

OCA at 07:31, 12 November 2014

2014-11-12T07:31:11Z

←Older revision		Revision as of 07:31, 12 November 2014
Line 2:		Line 2:
	<StructureSection load='3tpf' size='340' side='right' caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3tpf' size='340' side='right' caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c9y\|1c9y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c9y\|1c9y]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32022 Campylobacter jejuni subsp. jejuni])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum]</span></td></tr>
Line 25:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Campylobacter jejuni subsp. jejuni]]
	[[Category: Ornithine carbamoyltransferase]]		[[Category: Ornithine carbamoyltransferase]]
-	[[Category: Anderson, W F.]]	+	[[Category: Anderson, W F]]
-	[[Category: ~~CSGID, Center for~~ Structural ~~Genomics of Infectious Diseases.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Chruszcz, M.]]	+	[[Category: Chruszcz, M]]
-	[[Category: Grabowski, M.]]	+	[[Category: Grabowski, M]]
-	[[Category: Grimshaw, S.]]	+	[[Category: Grimshaw, S]]
-	[[Category: Minor, W.]]	+	[[Category: Minor, W]]
-	[[Category: Onopriyenko, O.]]	+	[[Category: Onopriyenko, O]]
-	[[Category: Porebski, P J.]]	+	[[Category: Porebski, P J]]
-	[[Category: Savchenko, A.]]	+	[[Category: Savchenko, A]]
-	[[Category: Shabalin, I G.]]	+	[[Category: Shabalin, I G]]
	[[Category: Carbamoyl phosphate binding]]		[[Category: Carbamoyl phosphate binding]]
-	[[Category: Center for structural genomics of infectious disease]]
	[[Category: Csgid]]		[[Category: Csgid]]
	[[Category: L-ornithine binding]]		[[Category: L-ornithine binding]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
-	[[Category: Structural genomic]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:18, 3 November 2014

2014-11-03T14:18:52Z

←Older revision		Revision as of 14:18, 3 November 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c9y\|1c9y]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c9y\|1c9y]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32022 Campylobacter jejuni subsp. jejuni])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32022 Campylobacter jejuni subsp. jejuni])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 41:		Line 41:
	[[Category: Csgid]]		[[Category: Csgid]]
	[[Category: L-ornithine binding]]		[[Category: L-ornithine binding]]
-	[[Category: ~~Rossman~~ fold]]	+	[[Category: Rossmann fold]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 06:51, 25 June 2014

2014-06-25T06:51:06Z

←Older revision		Revision as of 06:51, 25 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3tpf.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168==
		+	<StructureSection load='3tpf' size='340' side='right' caption='[[3tpf]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tpf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1c9y\|1c9y]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argF, Cj0994c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32022 Campylobacter jejuni subsp. jejuni])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpf RCSB], [http://www.ebi.ac.uk/pdbsum/3tpf PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Anabolic ornithine transcarbamoylase (aOTC) catalyzes the reaction between carbamoyl phosphate (CP) and L-ornithine (ORN) to form L-citrulline and phosphate in the urea cycle and L-arginine biosynthesis. The crystal structure of unliganded aOTC from Campylobacter jejuni (Cje aOTC) was determined at 2.7 A resolution and refined to an R(work) of 20.3% and an R(free) of 24.0%. Cje aOTC is a trimer that forms a head-to-head pseudohexamer in the asymmetric unit. Each monomer is composed of an N-terminal CP-binding domain and a C-terminal ORN-binding domain joined by two interdomain helices. The Cje aOTC structure presents an open conformation of the enzyme with a relatively flexible orientation of the ORN-binding domain respective to the CP-binding domain. The conformation of the B2-H3 loop (residues 68-78), which is involved in binding CP in an adjacent subunit of the trimer, differs from that seen in homologous proteins with CP bound. The loop containing the ORN-binding motif (DxxxSMG, residues 223-230) has a conformation that is different from those observed in unliganded OTC structures from other species, but is similar to those in structures with bound ORN analogs. The major differences in tertiary structure between Cje aOTC and human aOTC are described.

-	~~{{STRUCTURE_3tpf\| PDB=3tpf \| SCENE= }}~~	+	Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 A resolution.,Shabalin IG, Porebski PJ, Cooper DR, Grabowski M, Onopriyenko O, Grimshaw S, Savchenko A, Chruszcz M, Minor W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep;68(Pt 9):1018-24. Epub, 2012 Aug 29. PMID:22949186<ref>PMID:22949186</ref>

-	~~===Crystal structure~~ of ~~anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp~~. ~~jejuni NCTC 11168===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22949186}}~~	+	==See Also==
-		+	*[[Ornithine carbamoyltransferase\|Ornithine carbamoyltransferase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3tpf~~]] ~~is a 6 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA].	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Campylobacter jejuni subsp. jejuni]]		[[Category: Campylobacter jejuni subsp. jejuni]]
	[[Category: Ornithine carbamoyltransferase]]		[[Category: Ornithine carbamoyltransferase]]

OCA at 06:23, 19 September 2012

2012-09-19T06:23:54Z

←Older revision		Revision as of 06:23, 19 September 2012
Line 1:		Line 1:
-	[[Image:3tpf.~~jpg~~\|left\|200px]]	+	[[Image:3tpf.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3tpf", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_3tpf\| PDB=3tpf \| SCENE= }}		{{STRUCTURE_3tpf\| PDB=3tpf \| SCENE= }}

	===Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168===		===Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168===

		+	{{ABSTRACT_PUBMED_22949186}}

	==About this Structure==		==About this Structure==

OCA at 06:16, 21 September 2011

2011-09-21T06:16:50Z

←Older revision		Revision as of 06:16, 21 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tpf.jpg\|left\|200px]]

-	The ~~entry 3tpf~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tpf", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tpf\| PDB=3tpf \| SCENE= }}

-	~~Authors: Onopriyenko, O~~.~~, Grimshaw, S., Porebski, P. J., Grabowski, M., Savchenko, A., Chruszcz, M., Anderson, W.F., Minor, W., Center for Structural Genomics of Infectious Diseases (CSGID)~~	+	===Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168===

-	~~Description: Crystal~~ structure ~~of anabolic ornithine carbamoyltransferase~~ from Campylobacter jejuni subsp. jejuni ~~NCTC 11168~~	+
		+	==About this Structure==
		+	[[3tpf]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPF OCA].
		+	[[Category: Campylobacter jejuni subsp. jejuni]]
		+	[[Category: Ornithine carbamoyltransferase]]
		+	[[Category: Anderson, W F.]]
		+	[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]
		+	[[Category: Chruszcz, M.]]
		+	[[Category: Grabowski, M.]]
		+	[[Category: Grimshaw, S.]]
		+	[[Category: Minor, W.]]
		+	[[Category: Onopriyenko, O.]]
		+	[[Category: Porebski, P J.]]
		+	[[Category: Savchenko, A.]]
		+	[[Category: Shabalin, I G.]]
		+	[[Category: Carbamoyl phosphate binding]]
		+	[[Category: Center for structural genomics of infectious disease]]
		+	[[Category: Csgid]]
		+	[[Category: L-ornithine binding]]
		+	[[Category: Rossman fold]]
		+	[[Category: Structural genomic]]
		+	[[Category: Transferase]]