3tq0 - Revision history

OCA at 13:36, 14 March 2024

2024-03-14T13:36:18Z

←Older revision		Revision as of 13:36, 14 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q4QEW7_LEIMA Q4QEW7_LEIMA]		[https://www.uniprot.org/uniprot/Q4QEW7_LEIMA Q4QEW7_LEIMA]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine biosynthetic pathway and has been exploited as the target for therapy against proliferative and parasitic diseases. In this study, we report the crystal structures of DHODH from Leishmania major, the species of Leishmania associated with zoonotic cutaneous leishmaniasis, in its apo form and in complex with orotate and fumarate molecules. Both orotate and fumarate were found to bind to the same active site and exploit similar interactions, consistent with a ping-pong mechanism described for class 1A DHODHs. Analysis of LmDHODH structures reveals that rearrangements in the conformation of the catalytic loop have direct influence on the dimeric interface. This is the first structural evidence of a relationship between the dimeric form and the catalytic mechanism. According to our analysis, the high sequence and structural similarity observed among trypanosomatid DHODH suggest that a single strategy of structure-based inhibitor design can be used to validate DHODH as a druggable target against multiple neglected tropical diseases such as Leishmaniasis, Sleeping sickness and Chagas' diseases.
-
-	Crystal structure of dihydroorotate dehydrogenase from Leishmania major.,Cordeiro AT, Feliciano PR, Pinheiro MP, Nonato MC Biochimie. 2012 Apr 21. PMID:22542640<ref>PMID:22542640</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3tq0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Dihydroorotate dehydrogenase 3D structures\|Dihydroorotate dehydrogenase 3D structures]]		*[[Dihydroorotate dehydrogenase 3D structures\|Dihydroorotate dehydrogenase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:06, 15 November 2023

2023-11-15T09:06:24Z

←Older revision		Revision as of 09:06, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='3tq0' size='340' side='right'caption='[[3tq0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3tq0' size='340' side='right'caption='[[3tq0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tq0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Leishmania_major_mhom/il/81/friedlin~~ Leishmania major ~~mhom/il/81/friedlin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TQ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tq0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major_strain_Friedlin Leishmania major strain Friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TQ0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DHODH, lmjf16.0530, LMJF_16_0530 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydroorotate_oxidase_(fumarate) Dihydroorotate oxidase (fumarate)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.98.1 1.3.98.1] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq0 OCA], [https://pdbe.org/3tq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tq0 RCSB], [https://www.ebi.ac.uk/pdbsum/3tq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tq0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq0 OCA], [https://pdbe.org/3tq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tq0 RCSB], [https://www.ebi.ac.uk/pdbsum/3tq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tq0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4QEW7_LEIMA Q4QEW7_LEIMA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Leishmania major ~~mhom/il/81/friedlin~~]]	+	[[Category: Leishmania major strain Friedlin]]
-	[[Category: Cordeiro~~, A T~~]]	+	[[Category: Cordeiro AT]]
-	[[Category: Feliciano~~, P R~~]]	+	[[Category: Feliciano PR]]
-	[[Category: Nonato~~, M C]]~~	+	[[Category: Nonato MC]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 17:08, 6 July 2022

2022-07-06T17:08:22Z

←Older revision		Revision as of 17:08, 6 July 2022
Line 1:		Line 1:

	==Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate==		==Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate==
-	<StructureSection load='3tq0' size='340' side='right' caption='[[3tq0]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3tq0' size='340' side='right'caption='[[3tq0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tq0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Leishmania_major_mhom/il/81/friedlin Leishmania major mhom/il/81/friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TQ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tq0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major_mhom/il/81/friedlin Leishmania major mhom/il/81/friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TQ0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DHODH, lmjf16.0530, LMJF_16_0530 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DHODH, lmjf16.0530, LMJF_16_0530 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Dihydroorotate_oxidase_(fumarate) Dihydroorotate oxidase (fumarate)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.98.1 1.3.98.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydroorotate_oxidase_(fumarate) Dihydroorotate oxidase (fumarate)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.98.1 1.3.98.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq0 OCA], [~~http~~://pdbe.org/3tq0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tq0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tq0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tq0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq0 OCA], [https://pdbe.org/3tq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tq0 RCSB], [https://www.ebi.ac.uk/pdbsum/3tq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tq0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Dihydroorotate dehydrogenase\|Dihydroorotate dehydrogenase]]	+	*[[Dihydroorotate dehydrogenase 3D structures\|Dihydroorotate dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Leishmania major mhom/il/81/friedlin]]		[[Category: Leishmania major mhom/il/81/friedlin]]
	[[Category: Cordeiro, A T]]		[[Category: Cordeiro, A T]]

OCA at 08:05, 11 August 2016

2016-08-11T08:05:03Z

←Older revision		Revision as of 08:05, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate==		==Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate==
	<StructureSection load='3tq0' size='340' side='right' caption='[[3tq0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3tq0' size='340' side='right' caption='[[3tq0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Leishmania_major~~ Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TQ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major_mhom/il/81/friedlin Leishmania major mhom/il/81/friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TQ0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DHODH, lmjf16.0530, LMJF_16_0530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~5664~~ Leishmania major])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DHODH, lmjf16.0530, LMJF_16_0530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Dihydroorotate_oxidase~~ Dihydroorotate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.1 1.3.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroorotate_oxidase_(fumarate) Dihydroorotate oxidase (fumarate)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.98.1 1.3.98.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tq0 RCSB], [http://www.ebi.ac.uk/pdbsum/3tq0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq0 OCA], [http://pdbe.org/3tq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tq0 RCSB], [http://www.ebi.ac.uk/pdbsum/3tq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tq0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tq0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	~~[[Category: Dihydroorotate oxidase]]~~	+	[[Category: Leishmania major mhom/il/81/friedlin]]
-	[[Category: Leishmania major]]	+
	[[Category: Cordeiro, A T]]		[[Category: Cordeiro, A T]]
	[[Category: Feliciano, P R]]		[[Category: Feliciano, P R]]
	[[Category: Nonato, M C]]		[[Category: Nonato, M C]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 10:11, 4 January 2015

2015-01-04T10:11:58Z

←Older revision		Revision as of 10:11, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TQ0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TQ0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DHODH, lmjf16.0530, LMJF_16_0530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 Leishmania major])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DHODH, lmjf16.0530, LMJF_16_0530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 Leishmania major])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroorotate_oxidase Dihydroorotate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.1 1.3.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroorotate_oxidase Dihydroorotate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.1 1.3.3.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tq0 RCSB], [http://www.ebi.ac.uk/pdbsum/3tq0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tq0 RCSB], [http://www.ebi.ac.uk/pdbsum/3tq0 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Crystal structure of dihydroorotate dehydrogenase from Leishmania major.,Cordeiro AT, Feliciano PR, Pinheiro MP, Nonato MC Biochimie. 2012 Apr 21. PMID:22542640<ref>PMID:22542640</ref>		Crystal structure of dihydroorotate dehydrogenase from Leishmania major.,Cordeiro AT, Feliciano PR, Pinheiro MP, Nonato MC Biochimie. 2012 Apr 21. PMID:22542640<ref>PMID:22542640</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

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	[[Category: Dihydroorotate oxidase]]		[[Category: Dihydroorotate oxidase]]
	[[Category: Leishmania major]]		[[Category: Leishmania major]]
-	[[Category: Cordeiro, A T.]]	+	[[Category: Cordeiro, A T]]
-	[[Category: Feliciano, P R.]]	+	[[Category: Feliciano, P R]]
-	[[Category: Nonato, M C.]]	+	[[Category: Nonato, M C]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 06:46, 5 June 2014

2014-06-05T06:46:20Z

←Older revision		Revision as of 06:46, 5 June 2014
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-	[[~~Image:~~3tq0.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate==
		+	<StructureSection load='3tq0' size='340' side='right' caption='[[3tq0]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TQ0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DHODH, lmjf16.0530, LMJF_16_0530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 Leishmania major])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroorotate_oxidase Dihydroorotate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.1 1.3.3.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tq0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tq0 RCSB], [http://www.ebi.ac.uk/pdbsum/3tq0 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine biosynthetic pathway and has been exploited as the target for therapy against proliferative and parasitic diseases. In this study, we report the crystal structures of DHODH from Leishmania major, the species of Leishmania associated with zoonotic cutaneous leishmaniasis, in its apo form and in complex with orotate and fumarate molecules. Both orotate and fumarate were found to bind to the same active site and exploit similar interactions, consistent with a ping-pong mechanism described for class 1A DHODHs. Analysis of LmDHODH structures reveals that rearrangements in the conformation of the catalytic loop have direct influence on the dimeric interface. This is the first structural evidence of a relationship between the dimeric form and the catalytic mechanism. According to our analysis, the high sequence and structural similarity observed among trypanosomatid DHODH suggest that a single strategy of structure-based inhibitor design can be used to validate DHODH as a druggable target against multiple neglected tropical diseases such as Leishmaniasis, Sleeping sickness and Chagas' diseases.

-	~~{{STRUCTURE_3tq0\| PDB=3tq0 \| SCENE= }}~~	+	Crystal structure of dihydroorotate dehydrogenase from Leishmania major.,Cordeiro AT, Feliciano PR, Pinheiro MP, Nonato MC Biochimie. 2012 Apr 21. PMID:22542640<ref>PMID:22542640</ref>

-	~~===Crystal structure~~ of ~~Leishmania major dihydroorotate dehydrogenase in complex with fumarate===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22542640}}~~	+	==See Also==
-		+	*[[Dihydroorotate dehydrogenase\|Dihydroorotate dehydrogenase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3tq0~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:022542640<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Dihydroorotate oxidase]]		[[Category: Dihydroorotate oxidase]]
	[[Category: Leishmania major]]		[[Category: Leishmania major]]

OCA at 06:58, 4 July 2012

2012-07-04T06:58:33Z

←Older revision		Revision as of 06:58, 4 July 2012
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-	[[Image:3tq0.~~jpg~~\|left\|200px]]	+	[[Image:3tq0.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3tq0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3tq0\| PDB=3tq0 \| SCENE= }}		{{STRUCTURE_3tq0\| PDB=3tq0 \| SCENE= }}

	===Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate===		===Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22542640}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22542640 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22542640}}		{{ABSTRACT_PUBMED_22542640}}

OCA at 09:16, 16 May 2012

2012-05-16T09:16:01Z

←Older revision		Revision as of 09:16, 16 May 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:3tq0.jpg\|left\|200px]]

-	The ~~entry 3tq0~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tq0", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tq0\| PDB=3tq0 \| SCENE= }}

-	~~Authors: Cordeiro, A.T., Feliciano, P.R., Nonato, M.C.~~	+	===Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate===

-	~~Description~~: ~~Crystal~~ structure of Leishmania major ~~dihydroorotate dehydrogenase in complex with fumarate~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22542640}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22542640 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22542640}}
		+
		+	==About this Structure==
		+	[[3tq0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TQ0 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022542640</ref><references group="xtra"/>
		+	[[Category: Dihydroorotate oxidase]]
		+	[[Category: Leishmania major]]
		+	[[Category: Cordeiro, A T.]]
		+	[[Category: Feliciano, P R.]]
		+	[[Category: Nonato, M C.]]
		+	[[Category: Oxidoreductase]]

OCA at 08:42, 30 October 2011

2011-10-30T08:42:04Z

←Older revision		Revision as of 08:42, 30 October 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3tq0 is ON HOLD	+	The entry 3tq0 is ON HOLD until Paper Publication

	Authors: Cordeiro, A.T., Feliciano, P.R., Nonato, M.C.		Authors: Cordeiro, A.T., Feliciano, P.R., Nonato, M.C.

	Description: Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate		Description: Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate

OCA at 05:32, 21 September 2011

2011-09-21T05:32:02Z

←Older revision		Revision as of 05:32, 21 September 2011
Line 3:		Line 3:
	The entry 3tq0 is ON HOLD		The entry 3tq0 is ON HOLD

-	Authors: Cordeiro, A.T, Feliciano, PR, Nonato, MC	+	Authors: Cordeiro, A.T., Feliciano, P.R., Nonato, M.C.

	Description: Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate		Description: Crystal structure of Leishmania major dihydroorotate dehydrogenase in complex with fumarate