3tvq - Revision history

OCA at 13:41, 14 March 2024

2024-03-14T13:41:03Z

←Older revision		Revision as of 13:41, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3tvq' size='340' side='right'caption='[[3tvq]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='3tvq' size='340' side='right'caption='[[3tvq]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"actinomyces_glaucescens"_preobrazhenskaya_in_gauze_et_al._1957 "actinomyces~~ glaucescens~~" preobrazhenskaya in gauze et al. 1957~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TVQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_glaucescens Streptomyces glaucescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TVQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DQH:(2R,3R)~~-~~2-(3~~,~~4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>DQH</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2rer\|2rer]]~~</~~div~~>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DQH:(2R,3R)-2-(3,4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>DQH</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tcmN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1907 "Actinomyces glaucescens" Preobrazhenskaya in Gauze et al. 1957])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvq OCA], [https://pdbe.org/3tvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tvq RCSB], [https://www.ebi.ac.uk/pdbsum/3tvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tvq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvq OCA], [https://pdbe.org/3tvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tvq RCSB], [https://www.ebi.ac.uk/pdbsum/3tvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tvq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TCMN_STRGA TCMN_STRGA]] The N-terminal domain enhances the formation of an early, partially cyclized intermediate, while the C-terminal domain catalyzes the 3-O-methylation of one or more later intermediates in the biosynthetic pathway. Catalyzes the methylation of tetracenomycin D3 (Tcm D3) to yield Tcm B3. Catalyzes as well the following side reactions: methylation of 8-O-methyl-Tcm D3 to yield Tcm E; and of 9-carboxymethyl-Tcm B3 to yield Tcm A2.	+	[https://www.uniprot.org/uniprot/TCMN_STRGA TCMN_STRGA] The N-terminal domain enhances the formation of an early, partially cyclized intermediate, while the C-terminal domain catalyzes the 3-O-methylation of one or more later intermediates in the biosynthetic pathway. Catalyzes the methylation of tetracenomycin D3 (Tcm D3) to yield Tcm B3. Catalyzes as well the following side reactions: methylation of 8-O-methyl-Tcm D3 to yield Tcm E; and of 9-carboxymethyl-Tcm B3 to yield Tcm A2.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Aromatic polyketides are biologically active natural products. Many important pharmaceuticals are derived from aromatic polyketides. Especially important in aromatic polyketide biosynthesis is the regiospecific cyclization of a linear, preassembled polyketide chain catalyzed by aromatase/cyclase (ARO/CYC), which serves as a key control point in aromatic ring formation. How different ARO/CYCs promote different cyclization patterns is not well understood. The whiE locus of Streptomyces coelicolor A3(2) is responsible for the biosynthesis of an aromatic polyketide precursor to the gray spore pigment. The WhiE ARO/CYC catalyzes the regiospecific C9-C14 and C7-C16 cyclization and aromatization of a 24-carbon polyketide chain. WhiE ARO/CYC shares a high degree of similarity to another nonreducing PKS ARO/CYC, TcmN ARO/CYC. This paper presents the apo crystal structure of WhiE ARO/CYC, and cocrystal structures of WhiE and TcmN ARO/CYCs bound with polycyclic aromatic compounds that mimic the respective ARO/CYC products. Site-directed mutagenesis coupled with in vitro PKS reconstitution assays was used to characterize the interior pocket residues of WhiE ARO/CYC. The results confirmed that the interior pocket of ARO/CYCs is a critical determinant of polyketide cyclization specificity. A unified ARO/CYC-mediated cyclization mechanism is proposed on the basis of these structural and functional results.	+
-		+
-	Insight into the Molecular Basis of Aromatic Polyketide Cyclization: Crystal Structure and in Vitro Characterization of WhiE-ORFVI.,Lee MY, Ames BD, Tsai SC Biochemistry. 2012 Mar 30. PMID:22432862<ref>PMID:22432862</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3tvq" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Actinomyces glaucescens preobrazhenskaya in gauze et al. 1957]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ames, B D~~]]	+	[[Category: Streptomyces glaucescens]]
-	[[Category: ~~Lee, M Y~~]]	+	[[Category: Ames BD]]
-	[[Category: ~~Tsai, S C]]~~	+	[[Category: Lee M-Y]]
-	~~[[Category: Aromatase]]~~	+	[[Category: Tsai S-C]]
-	~~[[Category: Cyclase]]~~	+
-	~~[[Category: Dihyroquercetin]]~~	+
-	~~[[Category: Helix~~-~~grip fold~~]]	+
-	[[Category: ~~Multifunctional cyclase~~-~~dehydratase-3-o-methyl transferase]]~~	+
-	~~[[Category: Taxifolin]]~~	+
-	~~[[Category: Tetracenomycin]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:47, 13 July 2022

2022-07-13T05:47:27Z

←Older revision		Revision as of 05:47, 13 July 2022
Line 1:		Line 1:

	==Crystal structure of TCM Aro/Cyc complexed with trans-dihidroquercetin==		==Crystal structure of TCM Aro/Cyc complexed with trans-dihidroquercetin==
-	<StructureSection load='3tvq' size='340' side='right' caption='[[3tvq]], [[Resolution\|resolution]] 1.67Å' scene=''>	+	<StructureSection load='3tvq' size='340' side='right'caption='[[3tvq]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tvq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"actinomyces_glaucescens"_preobrazhenskaya_in_gauze_et_al._1957 "actinomyces glaucescens" preobrazhenskaya in gauze et al. 1957]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TVQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_glaucescens"_preobrazhenskaya_in_gauze_et_al._1957 "actinomyces glaucescens" preobrazhenskaya in gauze et al. 1957]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TVQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DQH:(2R,3R)-2-(3,4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>DQH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DQH:(2R,3R)-2-(3,4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>DQH</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rer\|2rer]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2rer\|2rer]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tcmN ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1907 "Actinomyces glaucescens" Preobrazhenskaya in Gauze et al. 1957])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tcmN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1907 "Actinomyces glaucescens" Preobrazhenskaya in Gauze et al. 1957])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvq OCA], [~~http~~://pdbe.org/3tvq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tvq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tvq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tvq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvq OCA], [https://pdbe.org/3tvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tvq RCSB], [https://www.ebi.ac.uk/pdbsum/3tvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tvq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TCMN_STRGA TCMN_STRGA]] The N-terminal domain enhances the formation of an early, partially cyclized intermediate, while the C-terminal domain catalyzes the 3-O-methylation of one or more later intermediates in the biosynthetic pathway. Catalyzes the methylation of tetracenomycin D3 (Tcm D3) to yield Tcm B3. Catalyzes as well the following side reactions: methylation of 8-O-methyl-Tcm D3 to yield Tcm E; and of 9-carboxymethyl-Tcm B3 to yield Tcm A2.	+	[[https://www.uniprot.org/uniprot/TCMN_STRGA TCMN_STRGA]] The N-terminal domain enhances the formation of an early, partially cyclized intermediate, while the C-terminal domain catalyzes the 3-O-methylation of one or more later intermediates in the biosynthetic pathway. Catalyzes the methylation of tetracenomycin D3 (Tcm D3) to yield Tcm B3. Catalyzes as well the following side reactions: methylation of 8-O-methyl-Tcm D3 to yield Tcm E; and of 9-carboxymethyl-Tcm B3 to yield Tcm A2.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Actinomyces glaucescens preobrazhenskaya in gauze et al. 1957]]		[[Category: Actinomyces glaucescens preobrazhenskaya in gauze et al. 1957]]
		+	[[Category: Large Structures]]
	[[Category: Ames, B D]]		[[Category: Ames, B D]]
	[[Category: Lee, M Y]]		[[Category: Lee, M Y]]

OCA at 23:11, 4 August 2016

2016-08-04T23:11:19Z

←Older revision		Revision as of 23:11, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of TCM Aro/Cyc complexed with trans-dihidroquercetin==		==Crystal structure of TCM Aro/Cyc complexed with trans-dihidroquercetin==
	<StructureSection load='3tvq' size='340' side='right' caption='[[3tvq]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='3tvq' size='340' side='right' caption='[[3tvq]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tvq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_glaucescens Streptomyces~~ glaucescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TVQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tvq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_glaucescens"_preobrazhenskaya_in_gauze_et_al._1957 "actinomyces glaucescens" preobrazhenskaya in gauze et al. 1957]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TVQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DQH:(2R,3R)-2-(3,4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>DQH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DQH:(2R,3R)-2-(3,4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>DQH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rer\|2rer]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rer\|2rer]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tcmN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1907 ~~Streptomyces~~ glaucescens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tcmN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1907 "Actinomyces glaucescens" Preobrazhenskaya in Gauze et al. 1957])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tvq RCSB], [http://www.ebi.ac.uk/pdbsum/3tvq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvq OCA], [http://pdbe.org/3tvq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tvq RCSB], [http://www.ebi.ac.uk/pdbsum/3tvq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tvq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tvq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces~~ glaucescens]]	+	[[Category: Actinomyces glaucescens preobrazhenskaya in gauze et al. 1957]]
	[[Category: Ames, B D]]		[[Category: Ames, B D]]
	[[Category: Lee, M Y]]		[[Category: Lee, M Y]]

OCA at 20:33, 24 December 2014

2014-12-24T20:33:22Z

←Older revision		Revision as of 20:33, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tvq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_glaucescens Streptomyces glaucescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TVQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tvq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_glaucescens Streptomyces glaucescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TVQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DQH:(2R,3R)-2-(3,4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>DQH</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DQH:(2R,3R)-2-(3,4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>DQH</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rer\|2rer]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rer\|2rer]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tcmN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1907 Streptomyces glaucescens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tcmN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1907 Streptomyces glaucescens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tvq RCSB], [http://www.ebi.ac.uk/pdbsum/3tvq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tvq RCSB], [http://www.ebi.ac.uk/pdbsum/3tvq PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TCMN_STRGA TCMN_STRGA]] The N-terminal domain enhances the formation of an early, partially cyclized intermediate, while the C-terminal domain catalyzes the 3-O-methylation of one or more later intermediates in the biosynthetic pathway. Catalyzes the methylation of tetracenomycin D3 (Tcm D3) to yield Tcm B3. Catalyzes as well the following side reactions: methylation of 8-O-methyl-Tcm D3 to yield Tcm E; and of 9-carboxymethyl-Tcm B3 to yield Tcm A2.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	Insight into the Molecular Basis of Aromatic Polyketide Cyclization: Crystal Structure and in Vitro Characterization of WhiE-ORFVI.,Lee MY, Ames BD, Tsai SC Biochemistry. 2012 Mar 30. PMID:22432862<ref>PMID:22432862</ref>		Insight into the Molecular Basis of Aromatic Polyketide Cyclization: Crystal Structure and in Vitro Characterization of WhiE-ORFVI.,Lee MY, Ames BD, Tsai SC Biochemistry. 2012 Mar 30. PMID:22432862<ref>PMID:22432862</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces glaucescens]]		[[Category: Streptomyces glaucescens]]
-	[[Category: Ames, B D.]]	+	[[Category: Ames, B D]]
-	[[Category: Lee, M Y.]]	+	[[Category: Lee, M Y]]
-	[[Category: Tsai, S C.]]	+	[[Category: Tsai, S C]]
	[[Category: Aromatase]]		[[Category: Aromatase]]
	[[Category: Cyclase]]		[[Category: Cyclase]]

OCA at 06:11, 5 June 2014

2014-06-05T06:11:10Z

←Older revision		Revision as of 06:11, 5 June 2014
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-	[[~~Image:~~3tvq.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of TCM Aro/Cyc complexed with trans-dihidroquercetin==
		+	<StructureSection load='3tvq' size='340' side='right' caption='[[3tvq]], [[Resolution\|resolution]] 1.67Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tvq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_glaucescens Streptomyces glaucescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TVQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DQH:(2R,3R)-2-(3,4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-2,3-DIHYDRO-4H-CHROMEN-4-ONE'>DQH</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rer\|2rer]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tcmN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1907 Streptomyces glaucescens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tvq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tvq RCSB], [http://www.ebi.ac.uk/pdbsum/3tvq PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aromatic polyketides are biologically active natural products. Many important pharmaceuticals are derived from aromatic polyketides. Especially important in aromatic polyketide biosynthesis is the regiospecific cyclization of a linear, preassembled polyketide chain catalyzed by aromatase/cyclase (ARO/CYC), which serves as a key control point in aromatic ring formation. How different ARO/CYCs promote different cyclization patterns is not well understood. The whiE locus of Streptomyces coelicolor A3(2) is responsible for the biosynthesis of an aromatic polyketide precursor to the gray spore pigment. The WhiE ARO/CYC catalyzes the regiospecific C9-C14 and C7-C16 cyclization and aromatization of a 24-carbon polyketide chain. WhiE ARO/CYC shares a high degree of similarity to another nonreducing PKS ARO/CYC, TcmN ARO/CYC. This paper presents the apo crystal structure of WhiE ARO/CYC, and cocrystal structures of WhiE and TcmN ARO/CYCs bound with polycyclic aromatic compounds that mimic the respective ARO/CYC products. Site-directed mutagenesis coupled with in vitro PKS reconstitution assays was used to characterize the interior pocket residues of WhiE ARO/CYC. The results confirmed that the interior pocket of ARO/CYCs is a critical determinant of polyketide cyclization specificity. A unified ARO/CYC-mediated cyclization mechanism is proposed on the basis of these structural and functional results.

-	~~<!--~~	+	Insight into the Molecular Basis of Aromatic Polyketide Cyclization: Crystal Structure and in Vitro Characterization of WhiE-ORFVI.,Lee MY, Ames BD, Tsai SC Biochemistry. 2012 Mar 30. PMID:22432862<ref>PMID:22432862</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3tvq", creates~~ the "Structure ~~Box" on the page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3tvq\| PDB=3tvq \| SCENE= }}~~	+

-	~~===Crystal structure of TCM Aro~~/~~Cyc complexed with trans-dihidroquercetin===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22432862}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22432862 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22432862}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3tvq]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Streptomyces_glaucescens Streptomyces glaucescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022432862<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces glaucescens]]		[[Category: Streptomyces glaucescens]]
	[[Category: Ames, B D.]]		[[Category: Ames, B D.]]

OCA at 08:23, 25 April 2012

2012-04-25T08:23:06Z

←Older revision		Revision as of 08:23, 25 April 2012
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-	[[Image:3tvq.~~jpg~~\|left\|200px]]	+	[[Image:3tvq.png\|left\|200px]]

	<!--		<!--

OCA at 06:07, 4 April 2012

2012-04-04T06:07:35Z

←Older revision		Revision as of 06:07, 4 April 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:3tvq.jpg\|left\|200px]]

-	The ~~entry 3tvq~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tvq", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tvq\| PDB=3tvq \| SCENE= }}

-	~~Authors: Ames, B.D., Lee, M.~~-~~Y., Tsai, S.-C.~~	+	===Crystal structure of TCM Aro/Cyc complexed with trans-dihidroquercetin===

-	~~Description~~: ~~Crystal structure of TCM Aro~~/~~Cyc complexed~~ with ~~trans~~-~~dihidroquercetin~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22432862}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22432862 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22432862}}
		+
		+	==About this Structure==
		+	[[3tvq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_glaucescens Streptomyces glaucescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TVQ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022432862</ref><references group="xtra"/>
		+	[[Category: Streptomyces glaucescens]]
		+	[[Category: Ames, B D.]]
		+	[[Category: Lee, M Y.]]
		+	[[Category: Tsai, S C.]]
		+	[[Category: Aromatase]]
		+	[[Category: Cyclase]]
		+	[[Category: Dihyroquercetin]]
		+	[[Category: Helix-grip fold]]
		+	[[Category: Multifunctional cyclase-dehydratase-3-o-methyl transferase]]
		+	[[Category: Taxifolin]]
		+	[[Category: Tetracenomycin]]
		+	[[Category: Transferase]]

OCA at 08:44, 30 October 2011

2011-10-30T08:44:28Z

←Older revision		Revision as of 08:44, 30 October 2011
Line 5:		Line 5:
	Authors: Ames, B.D., Lee, M.-Y., Tsai, S.-C.		Authors: Ames, B.D., Lee, M.-Y., Tsai, S.-C.

-	Description: ~~CRYSTAL STRUCTURE OF~~ TCM ~~ARO~~/~~CYC COMPLEXED WITH TRANS~~-~~DIHIDROQUERCETIN~~	+	Description: Crystal structure of TCM Aro/Cyc complexed with trans-dihidroquercetin

OCA: Protected "3tvq" [edit=sysop:move=sysop]

2011-09-28T05:18:29Z

Protected "3tvq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 28 September 2011

OCA: New page: '''Unreleased structure''' The entry 3tvq is ON HOLD Authors: Ames, B.D., Lee, M.-Y., Tsai, S.-C. Description: CRYSTAL STRUCTURE OF TCM ARO/CYC COMPLEXED WITH TRANS-DIHIDROQUERCETIN

2011-09-28T05:18:28Z

New page: '''Unreleased structure''' The entry 3tvq is ON HOLD Authors: Ames, B.D., Lee, M.-Y., Tsai, S.-C. Description: CRYSTAL STRUCTURE OF TCM ARO/CYC COMPLEXED WITH TRANS-DIHIDROQUERCETIN

New page

'''Unreleased structure'''

The entry 3tvq is ON HOLD

Authors: Ames, B.D., Lee, M.-Y., Tsai, S.-C.

Description: CRYSTAL STRUCTURE OF TCM ARO/CYC COMPLEXED WITH TRANS-DIHIDROQUERCETIN