3u3r - Revision history

OCA at 10:16, 1 March 2024

2024-03-01T10:16:52Z

←Older revision		Revision as of 10:16, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3u3r' size='340' side='right'caption='[[3u3r]], [[Resolution\|resolution]] 2.36Å' scene=''>		<StructureSection load='3u3r' size='340' side='right'caption='[[3u3r]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u3r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U3R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u3r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U3R FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=A3P:ADENOSINE~~-~~3-5-DIPHOSPHATE'>A3P</scene>~~, ~~<scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.36Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3u3j\|3u3j]], [[3u3k\|3u3k]], [[3u3m\|3u3m]], [[3u3o\|3u3o]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>hSULT1A1 D249G mutation, OK/SW-cl.88, STP, STP1, SULT1A1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3r OCA], [https://pdbe.org/3u3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u3r RCSB], [https://www.ebi.ac.uk/pdbsum/3u3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3r ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3r OCA], [https://pdbe.org/3u3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u3r RCSB], [https://www.ebi.ac.uk/pdbsum/3u3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3r ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>	+	[https://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Cytosolic sulfotransferases (SULTs) are mammalian enzymes that detoxify a wide variety of chemicals through the addition of a sulfate group. Despite extensive research, the molecular basis for the broad specificity of SULTs is still not understood. Here, structural, protein engineering and kinetic approaches were employed to obtain deep understanding of the molecular basis for the broad specificity, catalytic activity and substrate inhibition of SULT1A1. We have determined five new structures of SULT1A1 in complex with different acceptors, and utilized a directed evolution approach to generate SULT1A1 mutants with enhanced thermostability and increased catalytic activity. We found that active site plasticity enables binding of different acceptors and identified dramatic structural changes in the SULT1A1 active site leading to the binding of a second acceptor molecule in a conserved yet non-productive manner. Our combined approach highlights the dominant role of SULT1A1 structural flexibility in controlling the specificity and activity of this enzyme.	+
-		+
-	The molecular basis for the broad substrate specificity of human sulfotransferase 1A1.,Berger I, Guttman C, Amar D, Zarivach R, Aharoni A PLoS One. 2011;6(11):e26794. Epub 2011 Nov 1. PMID:22069470<ref>PMID:22069470</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3u3r" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 28:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aryl sulfotransferase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aharoni, A]]	+	[[Category: Aharoni A]]
-	[[Category: Berger, I]]	+	[[Category: Berger I]]
-	[[Category: Guttman, C]]	+	[[Category: Guttman C]]
-	[[Category: Zarivach, R]]	+	[[Category: Zarivach R]]
-	~~[[Category: Arylsulfotransferase]]~~	+
-	~~[[Category: P-nitrophenol]]~~	+
-	~~[[Category: Pap]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Xenobiotic~~]]	+

OCA at 05:56, 13 July 2022

2022-07-13T05:56:28Z

←Older revision		Revision as of 05:56, 13 July 2022
Line 1:		Line 1:

	==Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL==		==Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL==
-	<StructureSection load='3u3r' size='340' side='right' caption='[[3u3r]], [[Resolution\|resolution]] 2.36Å' scene=''>	+	<StructureSection load='3u3r' size='340' side='right'caption='[[3u3r]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u3r]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3U3R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u3r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U3R FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u3j\|3u3j]], [[3u3k\|3u3k]], [[3u3m\|3u3m]], [[3u3o\|3u3o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3u3j\|3u3j]], [[3u3k\|3u3k]], [[3u3m\|3u3m]], [[3u3o\|3u3o]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hSULT1A1 D249G mutation, OK/SW-cl.88, STP, STP1, SULT1A1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hSULT1A1 D249G mutation, OK/SW-cl.88, STP, STP1, SULT1A1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3u3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3r OCA], [~~http~~://pdbe.org/3u3r PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3u3r RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3u3r PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3r ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3r OCA], [https://pdbe.org/3u3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u3r RCSB], [https://www.ebi.ac.uk/pdbsum/3u3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3r ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>	+	[[https://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Sulfotransferase\|Sulfotransferase]]	+	*[[Sulfotransferase 3D structures\|Sulfotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	[[Category: Aryl sulfotransferase]]		[[Category: Aryl sulfotransferase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Aharoni, A]]		[[Category: Aharoni, A]]
	[[Category: Berger, I]]		[[Category: Berger, I]]

OCA at 02:21, 5 August 2016

2016-08-05T02:21:54Z

←Older revision		Revision as of 02:21, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL==		==Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL==
	<StructureSection load='3u3r' size='340' side='right' caption='[[3u3r]], [[Resolution\|resolution]] 2.36Å' scene=''>		<StructureSection load='3u3r' size='340' side='right' caption='[[3u3r]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u3r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u3r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3R FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u3j\|3u3j]], [[3u3k\|3u3k]], [[3u3m\|3u3m]], [[3u3o\|3u3o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u3j\|3u3j]], [[3u3k\|3u3k]], [[3u3m\|3u3m]], [[3u3o\|3u3o]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hSULT1A1 D249G mutation, OK/SW-cl.88, STP, STP1, SULT1A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hSULT1A1 D249G mutation, OK/SW-cl.88, STP, STP1, SULT1A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3r RCSB], [http://www.ebi.ac.uk/pdbsum/3u3r PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3r OCA], [http://pdbe.org/3u3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3u3r RCSB], [http://www.ebi.ac.uk/pdbsum/3u3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3r ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3u3r" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Aryl sulfotransferase]]		[[Category: Aryl sulfotransferase]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Aharoni, A]]		[[Category: Aharoni, A]]
	[[Category: Berger, I]]		[[Category: Berger, I]]

OCA at 21:59, 24 December 2014

2014-12-24T21:59:40Z

←Older revision		Revision as of 21:59, 24 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3r RCSB], [http://www.ebi.ac.uk/pdbsum/3u3r PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3r RCSB], [http://www.ebi.ac.uk/pdbsum/3u3r PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:47, 9 December 2014

2014-12-09T17:47:03Z

←Older revision		Revision as of 17:47, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3u3r.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL==
		+	<StructureSection load='3u3r' size='340' side='right' caption='[[3u3r]], [[Resolution\|resolution]] 2.36Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3u3r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3R FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u3j\|3u3j]], [[3u3k\|3u3k]], [[3u3m\|3u3m]], [[3u3o\|3u3o]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hSULT1A1 D249G mutation, OK/SW-cl.88, STP, STP1, SULT1A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3r RCSB], [http://www.ebi.ac.uk/pdbsum/3u3r PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytosolic sulfotransferases (SULTs) are mammalian enzymes that detoxify a wide variety of chemicals through the addition of a sulfate group. Despite extensive research, the molecular basis for the broad specificity of SULTs is still not understood. Here, structural, protein engineering and kinetic approaches were employed to obtain deep understanding of the molecular basis for the broad specificity, catalytic activity and substrate inhibition of SULT1A1. We have determined five new structures of SULT1A1 in complex with different acceptors, and utilized a directed evolution approach to generate SULT1A1 mutants with enhanced thermostability and increased catalytic activity. We found that active site plasticity enables binding of different acceptors and identified dramatic structural changes in the SULT1A1 active site leading to the binding of a second acceptor molecule in a conserved yet non-productive manner. Our combined approach highlights the dominant role of SULT1A1 structural flexibility in controlling the specificity and activity of this enzyme.

-	~~{{STRUCTURE_3u3r\| PDB=3u3r \| SCENE= }}~~	+	The molecular basis for the broad substrate specificity of human sulfotransferase 1A1.,Berger I, Guttman C, Amar D, Zarivach R, Aharoni A PLoS One. 2011;6(11):e26794. Epub 2011 Nov 1. PMID:22069470<ref>PMID:22069470</ref>

-	~~===Crystal structure~~ of ~~D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22069470}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3u3r]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA].~~	+

	==See Also==		==See Also==
	*[[Sulfotransferase\|Sulfotransferase]]		*[[Sulfotransferase\|Sulfotransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022069470<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Aryl sulfotransferase]]		[[Category: Aryl sulfotransferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Aharoni, A.]]	+	[[Category: Aharoni, A]]
-	[[Category: Berger, I.]]	+	[[Category: Berger, I]]
-	[[Category: Guttman, C.]]	+	[[Category: Guttman, C]]
-	[[Category: Zarivach, R.]]	+	[[Category: Zarivach, R]]
	[[Category: Arylsulfotransferase]]		[[Category: Arylsulfotransferase]]
	[[Category: P-nitrophenol]]		[[Category: P-nitrophenol]]

OCA at 09:33, 20 October 2012

2012-10-20T09:33:28Z

←Older revision		Revision as of 09:33, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3u3r]] is a 1 chain structure ~~of [[Sulfotransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA].	+	[[3u3r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA].

	==See Also==		==See Also==

OCA at 23:15, 25 July 2012

2012-07-25T23:15:57Z

←Older revision		Revision as of 23:15, 25 July 2012
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	[[Image:3u3r.png\|left\|200px]]		[[Image:3u3r.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3u3r", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3u3r\| PDB=3u3r \| SCENE= }}		{{STRUCTURE_3u3r\| PDB=3u3r \| SCENE= }}

	===Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL===		===Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22069470}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22069470 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22069470}}		{{ABSTRACT_PUBMED_22069470}}

	==About this Structure==		==About this Structure==
-	[[3u3r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA].	+	[[3u3r]] is a 1 chain structure of [[Sulfotransferase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA].
		+
		+	==See Also==
		+	*[[Sulfotransferase\|Sulfotransferase]]

	==Reference==		==Reference==

OCA at 06:10, 11 April 2012

2012-04-11T06:10:49Z

←Older revision		Revision as of 06:10, 11 April 2012
Line 1:		Line 1:
-	[[Image:3u3r.~~jpg~~\|left\|200px]]	+	[[Image:3u3r.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL===		===Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22069470}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22069470 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22069470}}

	==About this Structure==		==About this Structure==
	[[3u3r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA].		[[3u3r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022069470</ref><references group="xtra"/>
	[[Category: Aryl sulfotransferase]]		[[Category: Aryl sulfotransferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 12:32, 16 November 2011

2011-11-16T12:32:59Z

←Older revision		Revision as of 12:32, 16 November 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3u3r.jpg\|left\|200px]]

-	The ~~entry 3u3r~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3u3r", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3u3r\| PDB=3u3r \| SCENE= }}

-	~~Authors: Guttman, C., Berger, I., Aharoni, A., Zarivach, R.~~	+	===Crystal structure of D249G mutated Human SULT1A1 bound to PAP and P-NITROPHENOL===

-	~~Description: Crystal~~ structure ~~of D249G mutated Human SULT1A1 bound to PAP and~~ P-~~NITROPHENOL~~	+
		+	==About this Structure==
		+	[[3u3r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3R OCA].
		+	[[Category: Aryl sulfotransferase]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Aharoni, A.]]
		+	[[Category: Berger, I.]]
		+	[[Category: Guttman, C.]]
		+	[[Category: Zarivach, R.]]
		+	[[Category: Arylsulfotransferase]]
		+	[[Category: P-nitrophenol]]
		+	[[Category: Pap]]
		+	[[Category: Transferase]]
		+	[[Category: Xenobiotic]]

OCA: Protected "3u3r" [edit=sysop:move=sysop]

2011-10-30T08:49:59Z

Protected "3u3r" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:49, 30 October 2011