3u3y - Revision history

OCA at 10:16, 1 March 2024

2024-03-01T10:16:56Z

←Older revision		Revision as of 10:16, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3u3y' size='340' side='right'caption='[[3u3y]], [[Resolution\|resolution]] 2.28Å' scene=''>		<StructureSection load='3u3y' size='340' side='right'caption='[[3u3y]], [[Resolution\|resolution]] 2.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u3y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U3Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u3y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U3Y FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BU1:1,4~~-~~BUTANEDIOL'>BU1</scene>~~, ~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.28Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Trex1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10090 LK3 transgenic mice])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3y OCA], [https://pdbe.org/3u3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u3y RCSB], [https://www.ebi.ac.uk/pdbsum/3u3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3y OCA], [https://pdbe.org/3u3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u3y RCSB], [https://www.ebi.ac.uk/pdbsum/3u3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TREX1_MOUSE TREX1_MOUSE]] Exonuclease with a preference for double stranded DNA with mismatched 3' termini. May play a role in DNA repair.<ref>PMID:10391904</ref> <ref>PMID:11279105</ref>	+	[https://www.uniprot.org/uniprot/TREX1_MOUSE TREX1_MOUSE] Exonuclease with a preference for double stranded DNA with mismatched 3' termini. May play a role in DNA repair.<ref>PMID:10391904</ref> <ref>PMID:11279105</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Mutations within the human TREX1 3' exonuclease are associated with Aicardi-Goutieres Syndrome (AGS) and familial chilblain lupus (FCL). Both AGS and FCL are autoimmune diseases that result in increased levels of interferon alpha and circulating antibodies to DNA. TREX1 is a member of the endoplasmic reticulum (ER)-associated SET complex and participates in granzyme A-mediated cell death to degrade nicked genomic DNA. The loss of TREX1 activity may result in the accumulation of double-stranded DNA (dsDNA) degradation intermediates that trigger autoimmune activation. The X-ray crystal structures of the TREX1 wt apoprotein, the dominant D200H, D200N and D18N homodimer mutants derived from AGS and FCL patients, as well as the recessive V201D homodimer mutant have been determined. The structures of the D200H and D200N mutant proteins reveal the enzyme has lost coordination of one of the active site metals, and the catalytic histidine (H195) is trapped in a conformation pointing away from the active site. The TREX1 D18N and V201D mutants are able to bind both metals in the active site, but with inter-metal distances that are larger than optimal for catalysis. Additionally, all of the mutant structures reveal a reduced mobility in the catalytic histidine, providing further explanation for the loss of catalytic activity. The structures of the mutant TREX1 proteins provide insight into the dysfunction relating to human disease. Additionally, the TREX1 apoprotein structure together with the previously determined wild type substrate and product structures allow us to propose a distinct mechanism for the TREX1 exonuclease.	+
-		+
-	Defects in DNA degradation revealed in crystal structures of TREX1 exonuclease mutations linked to autoimmune disease.,Bailey SL, Harvey S, Perrino FW, Hollis T DNA Repair (Amst). 2011 Nov 7. PMID:22071149<ref>PMID:22071149</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3u3y" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 27:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Exodeoxyribonuclease III]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Bailey~~, S L~~]]	+	[[Category: Bailey SL]]
-	[[Category: Harvey, S]]	+	[[Category: Harvey S]]
-	[[Category: Hollis, T]]	+	[[Category: Hollis T]]
-	[[Category: Perrino~~, F W]]~~	+	[[Category: Perrino FW]]
-	~~[[Category: 3' exonuclease]]~~	+
-	~~[[Category: Homodimer]]~~	+
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Rnase h fold~~]]	+

OCA at 05:56, 13 July 2022

2022-07-13T05:56:46Z

←Older revision		Revision as of 05:56, 13 July 2022
Line 1:		Line 1:

	==Mouse TREX1 D200H mutant==		==Mouse TREX1 D200H mutant==
-	<StructureSection load='3u3y' size='340' side='right' caption='[[3u3y]], [[Resolution\|resolution]] 2.28Å' scene=''>	+	<StructureSection load='3u3y' size='340' side='right'caption='[[3u3y]], [[Resolution\|resolution]] 2.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u3y]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3U3Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u3y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U3Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Trex1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Trex1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3u3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3y OCA], [~~http~~://pdbe.org/3u3y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3u3y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3u3y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3y OCA], [https://pdbe.org/3u3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u3y RCSB], [https://www.ebi.ac.uk/pdbsum/3u3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TREX1_MOUSE TREX1_MOUSE]] Exonuclease with a preference for double stranded DNA with mismatched 3' termini. May play a role in DNA repair.<ref>PMID:10391904</ref> <ref>PMID:11279105</ref>	+	[[https://www.uniprot.org/uniprot/TREX1_MOUSE TREX1_MOUSE]] Exonuclease with a preference for double stranded DNA with mismatched 3' termini. May play a role in DNA repair.<ref>PMID:10391904</ref> <ref>PMID:11279105</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Exonuclease\|Exonuclease]]	+	*[[Exonuclease 3D structures\|Exonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Exodeoxyribonuclease III]]		[[Category: Exodeoxyribonuclease III]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Bailey, S L]]		[[Category: Bailey, S L]]

OCA at 10:07, 4 August 2016

2016-08-04T10:07:03Z

←Older revision		Revision as of 10:07, 4 August 2016
Line 1:		Line 1:
		+
	==Mouse TREX1 D200H mutant==		==Mouse TREX1 D200H mutant==
	<StructureSection load='3u3y' size='340' side='right' caption='[[3u3y]], [[Resolution\|resolution]] 2.28Å' scene=''>		<StructureSection load='3u3y' size='340' side='right' caption='[[3u3y]], [[Resolution\|resolution]] 2.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u3y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u3y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Trex1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Trex1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3y RCSB], [http://www.ebi.ac.uk/pdbsum/3u3y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3y OCA], [http://pdbe.org/3u3y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3u3y RCSB], [http://www.ebi.ac.uk/pdbsum/3u3y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3u3y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3u3y" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Exodeoxyribonuclease III]]		[[Category: Exodeoxyribonuclease III]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Bailey, S L]]		[[Category: Bailey, S L]]
	[[Category: Harvey, S]]		[[Category: Harvey, S]]

OCA at 08:43, 24 December 2014

2014-12-24T08:43:04Z

←Older revision		Revision as of 08:43, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3u3y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[3u3y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3Y FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Trex1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Trex1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3y RCSB], [http://www.ebi.ac.uk/pdbsum/3u3y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3y RCSB], [http://www.ebi.ac.uk/pdbsum/3u3y PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TREX1_MOUSE TREX1_MOUSE]] Exonuclease with a preference for double stranded DNA with mismatched 3' termini. May play a role in DNA repair.<ref>PMID:10391904</ref> <ref>PMID:11279105</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	Defects in DNA degradation revealed in crystal structures of TREX1 exonuclease mutations linked to autoimmune disease.,Bailey SL, Harvey S, Perrino FW, Hollis T DNA Repair (Amst). 2011 Nov 7. PMID:22071149<ref>PMID:22071149</ref>		Defects in DNA degradation revealed in crystal structures of TREX1 exonuclease mutations linked to autoimmune disease.,Bailey SL, Harvey S, Perrino FW, Hollis T DNA Repair (Amst). 2011 Nov 7. PMID:22071149<ref>PMID:22071149</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

Line 25:		Line 27:
	[[Category: Exodeoxyribonuclease III]]		[[Category: Exodeoxyribonuclease III]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Bailey, S L.]]	+	[[Category: Bailey, S L]]
-	[[Category: Harvey, S.]]	+	[[Category: Harvey, S]]
-	[[Category: Hollis, T.]]	+	[[Category: Hollis, T]]
-	[[Category: Perrino, F W.]]	+	[[Category: Perrino, F W]]
	[[Category: 3' exonuclease]]		[[Category: 3' exonuclease]]
	[[Category: Homodimer]]		[[Category: Homodimer]]
	[[Category: Hydrolase-dna complex]]		[[Category: Hydrolase-dna complex]]
	[[Category: Rnase h fold]]		[[Category: Rnase h fold]]

OCA at 06:07, 5 June 2014

2014-06-05T06:07:10Z

←Older revision		Revision as of 06:07, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3u3y.~~png~~\|~~left~~\|~~200px~~]]	+	==Mouse TREX1 D200H mutant==
		+	<StructureSection load='3u3y' size='340' side='right' caption='[[3u3y]], [[Resolution\|resolution]] 2.28Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3u3y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3Y FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Trex1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3y RCSB], [http://www.ebi.ac.uk/pdbsum/3u3y PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mutations within the human TREX1 3' exonuclease are associated with Aicardi-Goutieres Syndrome (AGS) and familial chilblain lupus (FCL). Both AGS and FCL are autoimmune diseases that result in increased levels of interferon alpha and circulating antibodies to DNA. TREX1 is a member of the endoplasmic reticulum (ER)-associated SET complex and participates in granzyme A-mediated cell death to degrade nicked genomic DNA. The loss of TREX1 activity may result in the accumulation of double-stranded DNA (dsDNA) degradation intermediates that trigger autoimmune activation. The X-ray crystal structures of the TREX1 wt apoprotein, the dominant D200H, D200N and D18N homodimer mutants derived from AGS and FCL patients, as well as the recessive V201D homodimer mutant have been determined. The structures of the D200H and D200N mutant proteins reveal the enzyme has lost coordination of one of the active site metals, and the catalytic histidine (H195) is trapped in a conformation pointing away from the active site. The TREX1 D18N and V201D mutants are able to bind both metals in the active site, but with inter-metal distances that are larger than optimal for catalysis. Additionally, all of the mutant structures reveal a reduced mobility in the catalytic histidine, providing further explanation for the loss of catalytic activity. The structures of the mutant TREX1 proteins provide insight into the dysfunction relating to human disease. Additionally, the TREX1 apoprotein structure together with the previously determined wild type substrate and product structures allow us to propose a distinct mechanism for the TREX1 exonuclease.

-	~~<!--~~	+	Defects in DNA degradation revealed in crystal structures of TREX1 exonuclease mutations linked to autoimmune disease.,Bailey SL, Harvey S, Perrino FW, Hollis T DNA Repair (Amst). 2011 Nov 7. PMID:22071149<ref>PMID:22071149</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3u3y"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3u3y\| PDB=3u3y \| SCENE= }}~~	+

-	~~===Mouse TREX1 D200H mutant===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Exonuclease\|Exonuclease]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22071149}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 22071149 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_22071149}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~3u3y~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022071149<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Exodeoxyribonuclease III]]		[[Category: Exodeoxyribonuclease III]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 07:01, 18 April 2012

2012-04-18T07:01:27Z

←Older revision		Revision as of 07:01, 18 April 2012
Line 1:		Line 1:
-	[[Image:3u3y.~~jpg~~\|left\|200px]]	+	[[Image:3u3y.png\|left\|200px]]

	<!--		<!--

OCA at 07:25, 28 December 2011

2011-12-28T07:25:17Z

←Older revision		Revision as of 07:25, 28 December 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3u3y.jpg\|left\|200px]]

-	The ~~entry 3u3y~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3u3y", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3u3y\| PDB=3u3y \| SCENE= }}

-	~~Authors: Bailey, S.L., Harvey, S., Perrino, F.W., Hollis, T.~~	+	===Mouse TREX1 D200H mutant===

-	~~Description~~: ~~Mouse TREX1 D200H mutant~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22071149}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22071149 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22071149}}
		+
		+	==About this Structure==
		+	[[3u3y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3Y OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022071149</ref><references group="xtra"/>
		+	[[Category: Exodeoxyribonuclease III]]
		+	[[Category: Mus musculus]]
		+	[[Category: Bailey, S L.]]
		+	[[Category: Harvey, S.]]
		+	[[Category: Hollis, T.]]
		+	[[Category: Perrino, F W.]]
		+	[[Category: 3' exonuclease]]
		+	[[Category: Homodimer]]
		+	[[Category: Hydrolase-dna complex]]
		+	[[Category: Rnase h fold]]

OCA: Protected "3u3y" [edit=sysop:move=sysop]

2011-10-30T08:50:11Z

Protected "3u3y" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:50, 30 October 2011

OCA: New page: '''Unreleased structure''' The entry 3u3y is ON HOLD Authors: Bailey, S.L., Harvey, S., Perrino, F.W., Hollis, T. Description: Mouse TREX1 D200H mutant

2011-10-30T08:50:10Z

New page: '''Unreleased structure''' The entry 3u3y is ON HOLD Authors: Bailey, S.L., Harvey, S., Perrino, F.W., Hollis, T. Description: Mouse TREX1 D200H mutant

New page

'''Unreleased structure'''

The entry 3u3y is ON HOLD

Authors: Bailey, S.L., Harvey, S., Perrino, F.W., Hollis, T.

Description: Mouse TREX1 D200H mutant