3uan - Revision history

OCA at 13:52, 14 March 2024

2024-03-14T13:52:49Z

←Older revision		Revision as of 13:52, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3uan' size='340' side='right'caption='[[3uan]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='3uan' size='340' side='right'caption='[[3uan]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uan]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uan]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UAN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=BDP:BETA-D-GLUCOPYRANURONIC+ACID'>BDP</scene>, <scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=NGY:2-(ACETYLAMINO)-2-DEOXY-6-O-SULFO-ALPHA-D-GLUCOPYRANOSE'>NGY</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.844Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zrh\|1zrh]], [[1t8t\|1t8t]], [[1t8u\|1t8u]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=BDP:BETA-D-GLUCOPYRANURONIC+ACID'>BDP</scene>, <scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=NGY:2-(ACETYLAMINO)-2-DEOXY-6-O-SULFO-ALPHA-D-GLUCOPYRANOSE'>NGY</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hs3st1, 3ost, 3ost1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uan OCA], [https://pdbe.org/3uan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uan RCSB], [https://www.ebi.ac.uk/pdbsum/3uan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uan ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uan OCA], [https://pdbe.org/3uan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uan RCSB], [https://www.ebi.ac.uk/pdbsum/3uan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uan ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HS3S1_MOUSE HS3S1_MOUSE]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.<ref>PMID:9346953</ref>	+	[https://www.uniprot.org/uniprot/HS3S1_MOUSE HS3S1_MOUSE] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.<ref>PMID:9346953</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Heparin is a polysaccharide-based natural product that is used clinically as an anticoagulant drug. Heparan sulfate 3-O-sulfotransferase (3-OST) is an enzyme that transfers a sulfo group to the 3-OH position of a glucosamine unit. 3-OST is present in multiple isoforms, and the polysaccharides modified by these different isoforms perform distinct biological functions. 3-OST isoform 1 (3-OST-1) is the key enzyme for the biosynthesis of anticoagulant heparin. Here, we report the crystal structure of the ternary complex of 3-OST-1, 3'-phosphoadenosine 5'-phosphate, and a heptasaccharide substrate. Comparisons to previously determined structures of 3-OST-3 reveal unique binding modes used by the different isoforms of 3-OST for distinguishing the fine structures of saccharide substrates. Our data demonstrate that the saccharide substrates display distinct conformations when interacting with the different 3-OST isoforms. Site-directed mutagenesis data suggest that several key amino residues, including Lys259, Thr256, and Trp283 in 3-OST-3 and Arg268 in 3-OST-1, play important roles in substrate binding and specificity between isoforms. These results deepen our understanding of the biosynthetic mechanism of heparan sulfate and provide structural information for engineering enzymes for an enhanced biosynthetic approach to heparin production.	+
-		+
-	Dissecting the substrate recognition of 3-O-sulfotransferase for the biosynthesis of anticoagulant heparin.,Moon AF, Xu Y, Woody SM, Krahn JM, Linhardt RJ, Liu J, Pedersen LC Proc Natl Acad Sci U S A. 2012 Mar 19. PMID:22431632<ref>PMID:22431632</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3uan" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 29:		Line 18:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Krahn~~, J M~~]]	+	[[Category: Krahn JM]]
-	[[Category: Linhardt~~, R J~~]]	+	[[Category: Linhardt RJ]]
-	[[Category: Liu, J]]	+	[[Category: Liu J]]
-	[[Category: Moon~~, A F~~]]	+	[[Category: Moon AF]]
-	[[Category: Pedersen~~, L C~~]]	+	[[Category: Pedersen LC]]
-	[[Category: Woody~~, S M~~]]	+	[[Category: Woody SM]]
-	[[Category: Xu, Y]]	+	[[Category: Xu Y]]
-	~~[[Category: Alpha/beta motif]]~~	+
-	~~[[Category: Co-factor paps/pap]]~~	+
-	~~[[Category: Golgi-localized]]~~	+
-	~~[[Category: Heparan sulfate]]~~	+
-	~~[[Category: Heparan sulfate oligosaccharide]]~~	+
-	~~[[Category: Sulfotransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:05, 13 July 2022

2022-07-13T06:05:29Z

←Older revision		Revision as of 06:05, 13 July 2022
Line 1:		Line 1:

	==Crystal structure of 3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate==		==Crystal structure of 3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate==
-	<StructureSection load='3uan' size='340' side='right' caption='[[3uan]], [[Resolution\|resolution]] 1.84Å' scene=''>	+	<StructureSection load='3uan' size='340' side='right'caption='[[3uan]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uan]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uan]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UAN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=BDP:BETA-D-GLUCOPYRANURONIC+ACID'>BDP</scene>, <scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=NGY:2-(ACETYLAMINO)-2-DEOXY-6-O-SULFO-ALPHA-D-GLUCOPYRANOSE'>NGY</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=BDP:BETA-D-GLUCOPYRANURONIC+ACID'>BDP</scene>, <scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=NGY:2-(ACETYLAMINO)-2-DEOXY-6-O-SULFO-ALPHA-D-GLUCOPYRANOSE'>NGY</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrh\|1zrh]], [[1t8t\|1t8t]], [[1t8u\|1t8u]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zrh\|1zrh]], [[1t8t\|1t8t]], [[1t8u\|1t8u]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hs3st1, 3ost, 3ost1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hs3st1, 3ost, 3ost1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3uan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uan OCA], [~~http~~://pdbe.org/3uan PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3uan RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3uan PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3uan ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uan OCA], [https://pdbe.org/3uan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uan RCSB], [https://www.ebi.ac.uk/pdbsum/3uan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uan ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HS3S1_MOUSE HS3S1_MOUSE]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.<ref>PMID:9346953</ref>	+	[[https://www.uniprot.org/uniprot/HS3S1_MOUSE HS3S1_MOUSE]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.<ref>PMID:9346953</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Sulfotransferase\|Sulfotransferase]]	+	*[[Sulfotransferase 3D structures\|Sulfotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Krahn, J M]]		[[Category: Krahn, J M]]

OCA at 22:01, 5 August 2016

2016-08-05T22:01:36Z

←Older revision		Revision as of 22:01, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of 3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate==		==Crystal structure of 3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate==
	<StructureSection load='3uan' size='340' side='right' caption='[[3uan]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='3uan' size='340' side='right' caption='[[3uan]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UAN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=BDP:BETA-D-GLUCOPYRANURONIC+ACID'>BDP</scene>, <scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=NGY:2-(ACETYLAMINO)-2-DEOXY-6-O-SULFO-ALPHA-D-GLUCOPYRANOSE'>NGY</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=BDP:BETA-D-GLUCOPYRANURONIC+ACID'>BDP</scene>, <scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=NGY:2-(ACETYLAMINO)-2-DEOXY-6-O-SULFO-ALPHA-D-GLUCOPYRANOSE'>NGY</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrh\|1zrh]], [[1t8t\|1t8t]], [[1t8u\|1t8u]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrh\|1zrh]], [[1t8t\|1t8t]], [[1t8u\|1t8u]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hs3st1, 3ost, 3ost1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hs3st1, 3ost, 3ost1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uan RCSB], [http://www.ebi.ac.uk/pdbsum/3uan PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uan OCA], [http://pdbe.org/3uan PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uan RCSB], [http://www.ebi.ac.uk/pdbsum/3uan PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uan ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3uan" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Krahn, J M]]		[[Category: Krahn, J M]]
	[[Category: Linhardt, R J]]		[[Category: Linhardt, R J]]

OCA at 06:12, 25 December 2014

2014-12-25T06:12:35Z

←Older revision		Revision as of 06:12, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uan RCSB], [http://www.ebi.ac.uk/pdbsum/3uan PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uan RCSB], [http://www.ebi.ac.uk/pdbsum/3uan PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HS3S1_MOUSE HS3S1_MOUSE]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.<ref>PMID:9346953</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:05, 9 December 2014

2014-12-09T16:05:37Z

←Older revision		Revision as of 16:05, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3uan.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of 3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate==
		+	<StructureSection load='3uan' size='340' side='right' caption='[[3uan]], [[Resolution\|resolution]] 1.84Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3uan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UAN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=BDP:BETA-D-GLUCOPYRANURONIC+ACID'>BDP</scene>, <scene name='pdbligand=IDS:2-O-SULFO-ALPHA-L-IDOPYRANURONIC+ACID'>IDS</scene>, <scene name='pdbligand=NGY:2-(ACETYLAMINO)-2-DEOXY-6-O-SULFO-ALPHA-D-GLUCOPYRANOSE'>NGY</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zrh\|1zrh]], [[1t8t\|1t8t]], [[1t8u\|1t8u]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hs3st1, 3ost, 3ost1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uan RCSB], [http://www.ebi.ac.uk/pdbsum/3uan PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Heparin is a polysaccharide-based natural product that is used clinically as an anticoagulant drug. Heparan sulfate 3-O-sulfotransferase (3-OST) is an enzyme that transfers a sulfo group to the 3-OH position of a glucosamine unit. 3-OST is present in multiple isoforms, and the polysaccharides modified by these different isoforms perform distinct biological functions. 3-OST isoform 1 (3-OST-1) is the key enzyme for the biosynthesis of anticoagulant heparin. Here, we report the crystal structure of the ternary complex of 3-OST-1, 3'-phosphoadenosine 5'-phosphate, and a heptasaccharide substrate. Comparisons to previously determined structures of 3-OST-3 reveal unique binding modes used by the different isoforms of 3-OST for distinguishing the fine structures of saccharide substrates. Our data demonstrate that the saccharide substrates display distinct conformations when interacting with the different 3-OST isoforms. Site-directed mutagenesis data suggest that several key amino residues, including Lys259, Thr256, and Trp283 in 3-OST-3 and Arg268 in 3-OST-1, play important roles in substrate binding and specificity between isoforms. These results deepen our understanding of the biosynthetic mechanism of heparan sulfate and provide structural information for engineering enzymes for an enhanced biosynthetic approach to heparin production.

-	~~{{STRUCTURE_3uan\| PDB=3uan \| SCENE= }}~~	+	Dissecting the substrate recognition of 3-O-sulfotransferase for the biosynthesis of anticoagulant heparin.,Moon AF, Xu Y, Woody SM, Krahn JM, Linhardt RJ, Liu J, Pedersen LC Proc Natl Acad Sci U S A. 2012 Mar 19. PMID:22431632<ref>PMID:22431632</ref>

-	~~===Crystal structure~~ of ~~3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22431632}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3uan]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA].~~	+

	==See Also==		==See Also==
	*[[Sulfotransferase\|Sulfotransferase]]		*[[Sulfotransferase\|Sulfotransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022431632<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Krahn, J M.]]	+	[[Category: Krahn, J M]]
-	[[Category: Linhardt, R J.]]	+	[[Category: Linhardt, R J]]
-	[[Category: Liu, J.]]	+	[[Category: Liu, J]]
-	[[Category: Moon, A F.]]	+	[[Category: Moon, A F]]
-	[[Category: Pedersen, L C.]]	+	[[Category: Pedersen, L C]]
-	[[Category: Woody, S M.]]	+	[[Category: Woody, S M]]
-	[[Category: Xu, Y.]]	+	[[Category: Xu, Y]]
	[[Category: Alpha/beta motif]]		[[Category: Alpha/beta motif]]
	[[Category: Co-factor paps/pap]]		[[Category: Co-factor paps/pap]]

OCA at 13:32, 20 October 2012

2012-10-20T13:32:57Z

←Older revision		Revision as of 13:32, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3uan]] is a 2 chain structure ~~of [[Sulfotransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA].	+	[[3uan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA].

	==See Also==		==See Also==

OCA at 12:19, 26 July 2012

2012-07-26T12:19:48Z

←Older revision		Revision as of 12:19, 26 July 2012
Line 1:		Line 1:
	[[Image:3uan.png\|left\|200px]]		[[Image:3uan.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3uan", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3uan\| PDB=3uan \| SCENE= }}		{{STRUCTURE_3uan\| PDB=3uan \| SCENE= }}

	===Crystal structure of 3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate===		===Crystal structure of 3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22431632}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22431632 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22431632}}		{{ABSTRACT_PUBMED_22431632}}

	==About this Structure==		==About this Structure==
-	[[3uan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA].	+	[[3uan]] is a 2 chain structure of [[Sulfotransferase]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA].
		+
		+	==See Also==
		+	*[[Sulfotransferase\|Sulfotransferase]]

	==Reference==		==Reference==

OCA at 06:56, 18 April 2012

2012-04-18T06:56:43Z

←Older revision		Revision as of 06:56, 18 April 2012
Line 1:		Line 1:
-	[[Image:3uan.~~jpg~~\|left\|200px]]	+	[[Image:3uan.png\|left\|200px]]

	<!--		<!--

OCA at 06:05, 4 April 2012

2012-04-04T06:05:37Z

←Older revision		Revision as of 06:05, 4 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3uan.jpg\|left\|200px]]

-	The ~~entry 3uan~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3uan", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3uan\| PDB=3uan \| SCENE= }}

-	~~Authors: Moon, A.F., Xu, Y., Woody, S.M., Krahn, J.M., Linhardt, R.J., Liu, J., Pedersen, L.C.~~	+	===Crystal structure of 3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate===

-	~~Description~~: ~~Sulfotransferase~~ with ~~bound substrate~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22431632}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22431632 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22431632}}
		+
		+	==About this Structure==
		+	[[3uan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAN OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022431632</ref><references group="xtra"/>
		+	[[Category: Mus musculus]]
		+	[[Category: Krahn, J M.]]
		+	[[Category: Linhardt, R J.]]
		+	[[Category: Liu, J.]]
		+	[[Category: Moon, A F.]]
		+	[[Category: Pedersen, L C.]]
		+	[[Category: Woody, S M.]]
		+	[[Category: Xu, Y.]]
		+	[[Category: Alpha/beta motif]]
		+	[[Category: Co-factor paps/pap]]
		+	[[Category: Golgi-localized]]
		+	[[Category: Heparan sulfate]]
		+	[[Category: Heparan sulfate oligosaccharide]]
		+	[[Category: Sulfotransferase]]
		+	[[Category: Transferase]]

OCA at 11:08, 14 December 2011

2011-12-14T11:08:33Z

←Older revision		Revision as of 11:08, 14 December 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3uan is ON HOLD	+	The entry 3uan is ON HOLD until Paper Publication

	Authors: Moon, A.F., Xu, Y., Woody, S.M., Krahn, J.M., Linhardt, R.J., Liu, J., Pedersen, L.C.		Authors: Moon, A.F., Xu, Y., Woody, S.M., Krahn, J.M., Linhardt, R.J., Liu, J., Pedersen, L.C.

	Description: Sulfotransferase with bound substrate		Description: Sulfotransferase with bound substrate