3ur7 - Revision history

OCA at 14:06, 14 March 2024

2024-03-14T14:06:44Z

←Older revision		Revision as of 14:06, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3ur7' size='340' side='right'caption='[[3ur7]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='3ur7' size='340' side='right'caption='[[3ur7]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ur7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Potato Potato~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UR7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ur7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UR7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1ghs\|1ghs]]~~, [[~~2cyg~~\|~~2cyg~~]]~~, [[3em5\|3em5]], [[3f55\|3f55]], [[3ur8\|3ur8]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>gluB20-2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~4113 Potato])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ur7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur7 OCA], [https://pdbe.org/3ur7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ur7 RCSB], [https://www.ebi.ac.uk/pdbsum/3ur7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ur7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ur7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur7 OCA], [https://pdbe.org/3ur7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ur7 RCSB], [https://www.ebi.ac.uk/pdbsum/3ur7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ur7 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q70C53_SOLTU Q70C53_SOLTU]
-	Endo-1,3-beta-glucanases are widely distributed among bacteria, fungi and higher plants. They are responsible for hydrolysis of the glycosidic bond in specific polysaccharides with tracts of unsubstituted beta-1,3-linked glucosyl residues. The plant enzymes belong to glycoside hydrolase family 17 (GH17) and are also members of class 2 of pathogenesis-related (PR) proteins. X-ray diffraction data were collected to 1.40 and 1.26 A resolution from two crystals of endo-1,3-beta-glucanase from Solanum tuberosum (potato, cultivar Desiree) which, despite having a similar packing framework, represented two separate crystal forms. In particular, they differed in the Matthews coefficient and are consequently referred to as higher density (HD; 1.40 A resolution) and lower density (LD; 1.26 A resolution) forms. The general fold of the protein resembles that of other known plant endo-1,3-beta-glucanases and is defined by a (beta/alpha)(8)-barrel with an additional subdomain built around the C-terminal half of the barrel. The structures revealed high flexibility of the subdomain, which forms part of the catalytic cleft. Comparison with structures of other GH17 endo-1,3-beta-glucanases revealed differences in the arrangement of the secondary-structure elements in this region, which can be correlated with sequence variability and may suggest distinct substrate-binding patterns. The crystal structures revealed an unusual packing mode, clearly visible in the LD structure, caused by the presence of the C-terminal His(6) tag, which extends from the compact fold of the enzyme molecule and docks in the catalytic cleft of a neighbouring molecule. In this way, an infinite chain of His-tag-linked protein molecules is formed along the c direction.	+
-		+
-	Two high-resolution structures of potato endo-1,3-beta-glucanase reveal subdomain flexibility with implications for substrate binding.,Wojtkowiak A, Witek K, Hennig J, Jaskolski M Acta Crystallogr D Biol Crystallogr. 2012 Jun;68(Pt 6):~~713-23. doi:, 10.1107~~/~~S090744491200995X. Epub 2012 May 17. PMID:22683794<ref>PMID:22683794<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3ur7" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]		*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Glucan endo-1,3-beta-D-glucosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Potato~~]]	+	[[Category: Solanum tuberosum]]
-	[[Category: Hennig, J]]	+	[[Category: Hennig J]]
-	[[Category: Jaskolski, M]]	+	[[Category: Jaskolski M]]
-	[[Category: Witek, K]]	+	[[Category: Witek K]]
-	[[Category: Wojtkowiak, A]]	+	[[Category: Wojtkowiak A]]
-	~~[[Category: Carbohydrate/sugar binding]]~~	+
-	~~[[Category: Gh17 family]]~~	+
-	~~[[Category: Glucoside hydrolase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 07:59, 20 July 2022

2022-07-20T07:59:44Z

←Older revision		Revision as of 07:59, 20 July 2022
Line 1:		Line 1:

	==Higher-density crystal structure of potato endo-1,3-beta-glucanase==		==Higher-density crystal structure of potato endo-1,3-beta-glucanase==
-	<StructureSection load='3ur7' size='340' side='right' caption='[[3ur7]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='3ur7' size='340' side='right'caption='[[3ur7]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ur7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Potato Potato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UR7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ur7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Potato Potato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UR7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ghs\|1ghs]], [[2cyg\|2cyg]], [[3em5\|3em5]], [[3f55\|3f55]], [[3ur8\|3ur8]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ghs\|1ghs]], [[2cyg\|2cyg]], [[3em5\|3em5]], [[3f55\|3f55]], [[3ur8\|3ur8]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gluB20-2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Potato])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gluB20-2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Potato])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ur7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur7 OCA], [~~http~~://pdbe.org/3ur7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ur7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ur7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ur7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ur7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur7 OCA], [https://pdbe.org/3ur7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ur7 RCSB], [https://www.ebi.ac.uk/pdbsum/3ur7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ur7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Glucanase\|Glucanase]]	+	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
		+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Glucan endo-1,3-beta-D-glucosidase]]		[[Category: Glucan endo-1,3-beta-D-glucosidase]]
		+	[[Category: Large Structures]]
	[[Category: Potato]]		[[Category: Potato]]
	[[Category: Hennig, J]]		[[Category: Hennig, J]]

OCA at 16:19, 11 August 2016

2016-08-11T16:19:08Z

←Older revision		Revision as of 16:19, 11 August 2016
Line 1:		Line 1:
		+
	==Higher-density crystal structure of potato endo-1,3-beta-glucanase==		==Higher-density crystal structure of potato endo-1,3-beta-glucanase==
	<StructureSection load='3ur7' size='340' side='right' caption='[[3ur7]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='3ur7' size='340' side='right' caption='[[3ur7]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ur7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Solanum_tuberosum Solanum tuberosum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UR7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ur7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Potato Potato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UR7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ghs\|1ghs]], [[2cyg\|2cyg]], [[3em5\|3em5]], [[3f55\|3f55]], [[3ur8\|3ur8]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ghs\|1ghs]], [[2cyg\|2cyg]], [[3em5\|3em5]], [[3f55\|3f55]], [[3ur8\|3ur8]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gluB20-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 ~~Solanum tuberosum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gluB20-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Potato])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ur7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ur7 RCSB], [http://www.ebi.ac.uk/pdbsum/3ur7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ur7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur7 OCA], [http://pdbe.org/3ur7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ur7 RCSB], [http://www.ebi.ac.uk/pdbsum/3ur7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ur7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ur7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Glucan endo-1,3-beta-D-glucosidase]]		[[Category: Glucan endo-1,3-beta-D-glucosidase]]
-	[[Category: ~~Solanum tuberosum~~]]	+	[[Category: Potato]]
	[[Category: Hennig, J]]		[[Category: Hennig, J]]
	[[Category: Jaskolski, M]]		[[Category: Jaskolski, M]]

OCA at 09:41, 4 January 2015

2015-01-04T09:41:08Z

←Older revision		Revision as of 09:41, 4 January 2015
Line 1:		Line 1:
-	[[~~Image:~~3ur7.~~png~~\|~~left~~\|~~200px~~]]	+	==Higher-density crystal structure of potato endo-1,3-beta-glucanase==
		+	<StructureSection load='3ur7' size='340' side='right' caption='[[3ur7]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ur7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UR7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ghs\|1ghs]], [[2cyg\|2cyg]], [[3em5\|3em5]], [[3f55\|3f55]], [[3ur8\|3ur8]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gluB20-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Solanum tuberosum])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ur7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ur7 RCSB], [http://www.ebi.ac.uk/pdbsum/3ur7 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Endo-1,3-beta-glucanases are widely distributed among bacteria, fungi and higher plants. They are responsible for hydrolysis of the glycosidic bond in specific polysaccharides with tracts of unsubstituted beta-1,3-linked glucosyl residues. The plant enzymes belong to glycoside hydrolase family 17 (GH17) and are also members of class 2 of pathogenesis-related (PR) proteins. X-ray diffraction data were collected to 1.40 and 1.26 A resolution from two crystals of endo-1,3-beta-glucanase from Solanum tuberosum (potato, cultivar Desiree) which, despite having a similar packing framework, represented two separate crystal forms. In particular, they differed in the Matthews coefficient and are consequently referred to as higher density (HD; 1.40 A resolution) and lower density (LD; 1.26 A resolution) forms. The general fold of the protein resembles that of other known plant endo-1,3-beta-glucanases and is defined by a (beta/alpha)(8)-barrel with an additional subdomain built around the C-terminal half of the barrel. The structures revealed high flexibility of the subdomain, which forms part of the catalytic cleft. Comparison with structures of other GH17 endo-1,3-beta-glucanases revealed differences in the arrangement of the secondary-structure elements in this region, which can be correlated with sequence variability and may suggest distinct substrate-binding patterns. The crystal structures revealed an unusual packing mode, clearly visible in the LD structure, caused by the presence of the C-terminal His(6) tag, which extends from the compact fold of the enzyme molecule and docks in the catalytic cleft of a neighbouring molecule. In this way, an infinite chain of His-tag-linked protein molecules is formed along the c direction.

-	~~{{STRUCTURE_3ur7\| PDB=3ur7 \| SCENE= }}~~	+	Two high-resolution structures of potato endo-1,3-beta-glucanase reveal subdomain flexibility with implications for substrate binding.,Wojtkowiak A, Witek K, Hennig J, Jaskolski M Acta Crystallogr D Biol Crystallogr. 2012 Jun;68(Pt 6):713-23. doi:, 10.1107/S090744491200995X. Epub 2012 May 17. PMID:22683794<ref>PMID:22683794</ref>

-	~~===Higher-density crystal structure of potato endo-1~~,~~3-beta-glucanase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22683794}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ur7]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Solanum_tuberosum Solanum tuberosum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA].~~	+

	==See Also==		==See Also==
-	*[[~~Beta-glucosidase~~\|~~Beta-glucosidase~~]]	+	*[[Glucanase\|Glucanase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016421930<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Glucan endo-1,3-beta-D-glucosidase]]		[[Category: Glucan endo-1,3-beta-D-glucosidase]]
	[[Category: Solanum tuberosum]]		[[Category: Solanum tuberosum]]
-	[[Category: Hennig, J.]]	+	[[Category: Hennig, J]]
-	[[Category: Jaskolski, M.]]	+	[[Category: Jaskolski, M]]
-	[[Category: Witek, K.]]	+	[[Category: Witek, K]]
-	[[Category: Wojtkowiak, A.]]	+	[[Category: Wojtkowiak, A]]
	[[Category: Carbohydrate/sugar binding]]		[[Category: Carbohydrate/sugar binding]]
	[[Category: Gh17 family]]		[[Category: Gh17 family]]

OCA at 11:42, 30 January 2013

2013-01-30T11:42:47Z

←Older revision		Revision as of 11:42, 30 January 2013
Line 9:		Line 9:
	==About this Structure==		==About this Structure==
	[[3ur7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA].		[[3ur7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA].
		+
		+	==See Also==
		+	*[[Beta-glucosidase\|Beta-glucosidase]]

	==Reference==		==Reference==

OCA at 11:52, 23 January 2013

2013-01-23T11:52:30Z

←Older revision		Revision as of 11:52, 23 January 2013
Line 1:		Line 1:
-	[[Image:3ur7.~~jpg~~\|left\|200px]]	+	[[Image:3ur7.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ur7", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ur7\| PDB=3ur7 \| SCENE= }}		{{STRUCTURE_3ur7\| PDB=3ur7 \| SCENE= }}

	===Higher-density crystal structure of potato endo-1,3-beta-glucanase===		===Higher-density crystal structure of potato endo-1,3-beta-glucanase===

-		+	{{ABSTRACT_PUBMED_22683794}}
-	~~<!--~~	+
-	~~The line below this paragraph,~~ {{~~ABSTRACT_PUBMED_19081847}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 19081847 is the PubMed ID number.~~	+
-	~~-->~~	+
-	~~{{ABSTRACT_PUBMED_19081847~~}}	+

	==About this Structure==		==About this Structure==
Line 22:		Line 11:

	==Reference==		==Reference==
-	~~<ref group="xtra">PMID:019081847</ref><ref group="xtra">PMID:008146192</ref>~~<ref group="xtra">PMID:016421930</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016421930</ref><references group="xtra"/>
	[[Category: Glucan endo-1,3-beta-D-glucosidase]]		[[Category: Glucan endo-1,3-beta-D-glucosidase]]
	[[Category: Solanum tuberosum]]		[[Category: Solanum tuberosum]]

OCA at 06:01, 30 May 2012

2012-05-30T06:01:22Z

←Older revision		Revision as of 06:01, 30 May 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ur7.jpg\|left\|200px]]

-	The ~~entry 3ur7~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ur7", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ur7\| PDB=3ur7 \| SCENE= }}

-	~~Authors: Wojtkowiak~~, ~~A., Witek, K., Hennig, J., Jaskolski, M.~~	+	===Higher-density crystal structure of potato endo-1,3-beta-glucanase===

-	~~Description~~: ~~Higher~~-~~density crystal~~ structure ~~of potato~~ endo-1,3-beta-~~glucanase~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19081847}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19081847 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19081847}}
		+
		+	==About this Structure==
		+	[[3ur7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR7 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:019081847</ref><ref group="xtra">PMID:008146192</ref><ref group="xtra">PMID:016421930</ref><references group="xtra"/>
		+	[[Category: Glucan endo-1,3-beta-D-glucosidase]]
		+	[[Category: Solanum tuberosum]]
		+	[[Category: Hennig, J.]]
		+	[[Category: Jaskolski, M.]]
		+	[[Category: Witek, K.]]
		+	[[Category: Wojtkowiak, A.]]
		+	[[Category: Carbohydrate/sugar binding]]
		+	[[Category: Gh17 family]]
		+	[[Category: Glucoside hydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Tim barrel]]

OCA at 06:29, 11 April 2012

2012-04-11T06:29:10Z

←Older revision		Revision as of 06:29, 11 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ur7 is ON HOLD	+	The entry 3ur7 is ON HOLD until Paper Publication

	Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M.		Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M.

	Description: Higher-density crystal structure of potato endo-1,3-beta-glucanase		Description: Higher-density crystal structure of potato endo-1,3-beta-glucanase

OCA: Protected "3ur7" [edit=sysop:move=sysop]

2011-11-30T05:55:26Z

Protected "3ur7" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:55, 30 November 2011

OCA: New page: '''Unreleased structure''' The entry 3ur7 is ON HOLD Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M. Description: Higher-density crystal structure of potato endo-1,3-beta...

2011-11-30T05:55:25Z

New page: '''Unreleased structure''' The entry 3ur7 is ON HOLD Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M. Description: Higher-density crystal structure of potato endo-1,3-beta...

New page

'''Unreleased structure'''

The entry 3ur7 is ON HOLD

Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M.

Description: Higher-density crystal structure of potato endo-1,3-beta-glucanase