3uw3 - Revision history

OCA at 14:10, 14 March 2024

2024-03-14T14:10:12Z

←Older revision		Revision as of 14:10, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3uw3' size='340' side='right'caption='[[3uw3]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3uw3' size='340' side='right'caption='[[3uw3]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uw3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Burta Burta~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UW3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uw3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_thailandensis_E264 Burkholderia thailandensis E264]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UW3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:~~1~~,2-ETHANEDIOL'>EDO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>asd, BTH_II0675 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=271848 BURTA])</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Aspartate-semialdehyde_dehydrogenase Aspartate-semialdehyde dehydrogenase]~~, ~~with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=1.2.1.11 1.2.1.11]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uw3 OCA], [https://pdbe.org/3uw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uw3 RCSB], [https://www.ebi.ac.uk/pdbsum/3uw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uw3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uw3 OCA], [https://pdbe.org/3uw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uw3 RCSB], [https://www.ebi.ac.uk/pdbsum/3uw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uw3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Q2T7H5_BURTA Q2T7H5_BURTA]] Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate (By similarity).[HAMAP-Rule:MF_02121]	+	[https://www.uniprot.org/uniprot/Q2T7H5_BURTA Q2T7H5_BURTA] Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate (By similarity).[HAMAP-Rule:MF_02121]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	BACKGROUND: The genus Burkholderia includes pathogenic gram-negative bacteria that cause melioidosis, glanders, and pulmonary infections of patients with cancer and cystic fibrosis. Drug resistance has made development of new antimicrobials critical. Many approaches to discovering new antimicrobials, such as structure-based drug design and whole cell phenotypic screens followed by lead refinement, require high-resolution structures of proteins essential to the parasite. METHODOLOGY/PRINCIPAL FINDINGS: We experimentally identified 406 putative essential genes in B. thailandensis, a low-virulence species phylogenetically similar to B. pseudomallei, the causative agent of melioidosis, using saturation-level transposon mutagenesis and next-generation sequencing (Tn-seq). We selected 315 protein products of these genes based on structure-determination criteria, such as excluding very large and/or integral membrane proteins, and entered them into the Seattle Structural Genomics Center for Infection Disease (SSGCID) structure determination pipeline. To maximize structural coverage of these targets, we applied an "ortholog rescue" strategy for those producing insoluble or difficult to crystallize proteins, resulting in the addition of 387 orthologs (or paralogs) from seven other Burkholderia species into the SSGCID pipeline. This structural genomics approach yielded structures from 31 putative essential targets from B. thailandensis, and 25 orthologs from other Burkholderia species, yielding an overall structural coverage for 49 of the 406 essential gene families, with a total of 88 depositions into the Protein Data Bank. Of these, 25 proteins have properties of a potential antimicrobial drug target i.e., no close human homolog, part of an essential metabolic pathway, and a deep binding pocket. We describe the structures of several potential drug targets in detail. CONCLUSIONS/SIGNIFICANCE: This collection of structures, solubility and experimental essentiality data provides a resource for development of drugs against infections and diseases caused by Burkholderia. All expression clones and proteins created in this study are freely available by request.	+
-		+
-	Combining functional and structural genomics to sample the essential Burkholderia structome.,Baugh L, Gallagher LA, Patrapuvich R, Clifton MC, Gardberg AS, Edwards TE, Armour B, Begley DW, Dieterich SH, Dranow DM, Abendroth J, Fairman JW, Fox D 3rd, Staker BL, Phan I, Gillespie A, Choi R, Nakazawa-Hewitt S, Nguyen MT, Napuli A, Barrett L, Buchko GW, Stacy R, Myler PJ, Stewart LJ, Manoil C, Van Voorhis WC PLoS One. 2013;8(1):e53851. doi: 10.1371/journal.pone.0053851. Epub 2013 Jan 31. PMID:23382856<ref>PMID:23382856</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3uw3" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]		*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
	*[[Aspartate-semialdehyde dehydrogenase 3D structures\|Aspartate-semialdehyde dehydrogenase 3D structures]]		*[[Aspartate-semialdehyde dehydrogenase 3D structures\|Aspartate-semialdehyde dehydrogenase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspartate-semialdehyde dehydrogenase]]~~	+	[[Category: Burkholderia thailandensis E264]]
-	~~[[Category: Burta~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Structural genomic]]
-	[[Category: Dehydrogenase]]
-	[[Category: Oxidoreductase]]

OCA at 08:11, 20 July 2022

2022-07-20T08:11:54Z

←Older revision		Revision as of 08:11, 20 July 2022
Line 1:		Line 1:

	==Crystal Structure of an Aspartate-Semialdehyde Dehydrogenase from Burkholderia Thailandensis==		==Crystal Structure of an Aspartate-Semialdehyde Dehydrogenase from Burkholderia Thailandensis==
-	<StructureSection load='3uw3' size='340' side='right' caption='[[3uw3]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='3uw3' size='340' side='right'caption='[[3uw3]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uw3]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Burta Burta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UW3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uw3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burta Burta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UW3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">asd, BTH_II0675 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 BURTA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">asd, BTH_II0675 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 BURTA])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Aspartate-semialdehyde_dehydrogenase Aspartate-semialdehyde dehydrogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.11 1.2.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aspartate-semialdehyde_dehydrogenase Aspartate-semialdehyde dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.11 1.2.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3uw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uw3 OCA], [~~http~~://pdbe.org/3uw3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3uw3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3uw3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3uw3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uw3 OCA], [https://pdbe.org/3uw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uw3 RCSB], [https://www.ebi.ac.uk/pdbsum/3uw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uw3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/Q2T7H5_BURTA Q2T7H5_BURTA]] Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate (By similarity).[HAMAP-Rule:MF_02121]	+	[[https://www.uniprot.org/uniprot/Q2T7H5_BURTA Q2T7H5_BURTA]] Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate (By similarity).[HAMAP-Rule:MF_02121]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]	+	*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
-	*[[Aspartate-semialdehyde dehydrogenase\|Aspartate-semialdehyde dehydrogenase]]	+	*[[Aspartate-semialdehyde dehydrogenase 3D structures\|Aspartate-semialdehyde dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	[[Category: Aspartate-semialdehyde dehydrogenase]]		[[Category: Aspartate-semialdehyde dehydrogenase]]
	[[Category: Burta]]		[[Category: Burta]]
		+	[[Category: Large Structures]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Dehydrogenase]]		[[Category: Dehydrogenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 14:20, 4 August 2016

2016-08-04T14:20:14Z

←Older revision		Revision as of 14:20, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of an Aspartate-Semialdehyde Dehydrogenase from Burkholderia Thailandensis==		==Crystal Structure of an Aspartate-Semialdehyde Dehydrogenase from Burkholderia Thailandensis==
	<StructureSection load='3uw3' size='340' side='right' caption='[[3uw3]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3uw3' size='340' side='right' caption='[[3uw3]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Burkholderia_thailandensis_e264 Burkholderia thailandensis e264~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UW3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burta Burta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UW3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">asd, BTH_II0675 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 ~~Burkholderia thailandensis E264~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">asd, BTH_II0675 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 BURTA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate-semialdehyde_dehydrogenase Aspartate-semialdehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.11 1.2.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate-semialdehyde_dehydrogenase Aspartate-semialdehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.11 1.2.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uw3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uw3 RCSB], [http://www.ebi.ac.uk/pdbsum/3uw3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uw3 OCA], [http://pdbe.org/3uw3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uw3 RCSB], [http://www.ebi.ac.uk/pdbsum/3uw3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uw3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3uw3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Aspartate-semialdehyde dehydrogenase]]		[[Category: Aspartate-semialdehyde dehydrogenase]]
-	[[Category: ~~Burkholderia thailandensis e264~~]]	+	[[Category: Burta]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Dehydrogenase]]		[[Category: Dehydrogenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 15:37, 24 December 2014

2014-12-24T15:37:52Z

←Older revision		Revision as of 15:37, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uw3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uw3 RCSB], [http://www.ebi.ac.uk/pdbsum/3uw3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uw3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uw3 RCSB], [http://www.ebi.ac.uk/pdbsum/3uw3 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q2T7H5_BURTA Q2T7H5_BURTA]] Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate (By similarity).[HAMAP-Rule:MF_02121]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:28, 21 December 2014

2014-12-21T07:28:35Z

←Older revision		Revision as of 07:28, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3uw3\| PDB=3uw3 \| SCENE= }}~~	+	==Crystal Structure of an Aspartate-Semialdehyde Dehydrogenase from Burkholderia Thailandensis==
-	===Crystal Structure of an Aspartate-Semialdehyde Dehydrogenase from Burkholderia Thailandensis===	+	<StructureSection load='3uw3' size='340' side='right' caption='[[3uw3]], [[Resolution\|resolution]] 1.55Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23382856}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3uw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_thailandensis_e264 Burkholderia thailandensis e264]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UW3 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">asd, BTH_II0675 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 Burkholderia thailandensis E264])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate-semialdehyde_dehydrogenase Aspartate-semialdehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.11 1.2.1.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uw3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uw3 RCSB], [http://www.ebi.ac.uk/pdbsum/3uw3 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: The genus Burkholderia includes pathogenic gram-negative bacteria that cause melioidosis, glanders, and pulmonary infections of patients with cancer and cystic fibrosis. Drug resistance has made development of new antimicrobials critical. Many approaches to discovering new antimicrobials, such as structure-based drug design and whole cell phenotypic screens followed by lead refinement, require high-resolution structures of proteins essential to the parasite. METHODOLOGY/PRINCIPAL FINDINGS: We experimentally identified 406 putative essential genes in B. thailandensis, a low-virulence species phylogenetically similar to B. pseudomallei, the causative agent of melioidosis, using saturation-level transposon mutagenesis and next-generation sequencing (Tn-seq). We selected 315 protein products of these genes based on structure-determination criteria, such as excluding very large and/or integral membrane proteins, and entered them into the Seattle Structural Genomics Center for Infection Disease (SSGCID) structure determination pipeline. To maximize structural coverage of these targets, we applied an "ortholog rescue" strategy for those producing insoluble or difficult to crystallize proteins, resulting in the addition of 387 orthologs (or paralogs) from seven other Burkholderia species into the SSGCID pipeline. This structural genomics approach yielded structures from 31 putative essential targets from B. thailandensis, and 25 orthologs from other Burkholderia species, yielding an overall structural coverage for 49 of the 406 essential gene families, with a total of 88 depositions into the Protein Data Bank. Of these, 25 proteins have properties of a potential antimicrobial drug target i.e., no close human homolog, part of an essential metabolic pathway, and a deep binding pocket. We describe the structures of several potential drug targets in detail. CONCLUSIONS/SIGNIFICANCE: This collection of structures, solubility and experimental essentiality data provides a resource for development of drugs against infections and diseases caused by Burkholderia. All expression clones and proteins created in this study are freely available by request.

-	~~==Function==~~	+	Combining functional and structural genomics to sample the essential Burkholderia structome.,Baugh L, Gallagher LA, Patrapuvich R, Clifton MC, Gardberg AS, Edwards TE, Armour B, Begley DW, Dieterich SH, Dranow DM, Abendroth J, Fairman JW, Fox D 3rd, Staker BL, Phan I, Gillespie A, Choi R, Nakazawa-Hewitt S, Nguyen MT, Napuli A, Barrett L, Buchko GW, Stacy R, Myler PJ, Stewart LJ, Manoil C, Van Voorhis WC PLoS One. 2013;8(1):e53851. doi: 10.1371/journal.pone.0053851. Epub 2013 Jan 31. PMID:23382856<ref>PMID:23382856</ref>
-	~~[[http://www~~.~~uniprot.org/uniprot/Q2T7H5_BURTA Q2T7H5_BURTA]] Catalyzes the NADPH-dependent formation of~~ L-~~aspartate-semialdehyde (~~L~~-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate~~ (~~By similarity~~).~~[HAMAP-Rule~~:~~MF_02121]~~	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3uw3]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Burkholderia_thailandensis_e264 Burkholderia thailandensis e264]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA].~~	+	</div>

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]		*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]
	*[[Aspartate-semialdehyde dehydrogenase\|Aspartate-semialdehyde dehydrogenase]]		*[[Aspartate-semialdehyde dehydrogenase\|Aspartate-semialdehyde dehydrogenase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023382856</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Aspartate-semialdehyde dehydrogenase]]		[[Category: Aspartate-semialdehyde dehydrogenase]]
	[[Category: Burkholderia thailandensis e264]]		[[Category: Burkholderia thailandensis e264]]
-	[[Category: ~~SSGCID, Seattle~~ Structural ~~Genomics Center for Infectious Disease.~~]]	+	[[Category: Structural genomic]]
	[[Category: Dehydrogenase]]		[[Category: Dehydrogenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-	[[Category: Structural genomic]]

OCA at 08:34, 30 October 2013

2013-10-30T08:34:02Z

←Older revision		Revision as of 08:34, 30 October 2013
Line 1:		Line 1:
-	[[Image:3uw3.png\|left\|200px]]
-
	{{STRUCTURE_3uw3\| PDB=3uw3 \| SCENE= }}		{{STRUCTURE_3uw3\| PDB=3uw3 \| SCENE= }}
-
	===Crystal Structure of an Aspartate-Semialdehyde Dehydrogenase from Burkholderia Thailandensis===		===Crystal Structure of an Aspartate-Semialdehyde Dehydrogenase from Burkholderia Thailandensis===
		+	{{ABSTRACT_PUBMED_23382856}}

		+	==Function==
		+	[[http://www.uniprot.org/uniprot/Q2T7H5_BURTA Q2T7H5_BURTA]] Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate (By similarity).[HAMAP-Rule:MF_02121]

	==About this Structure==		==About this Structure==
-	[[3uw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Burkholderia_thailandensis~~ Burkholderia thailandensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA].	+	[[3uw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_thailandensis_e264 Burkholderia thailandensis e264]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA].

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]		*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]
	*[[Aspartate-semialdehyde dehydrogenase\|Aspartate-semialdehyde dehydrogenase]]		*[[Aspartate-semialdehyde dehydrogenase\|Aspartate-semialdehyde dehydrogenase]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023382856</ref><references group="xtra"/><references/>
	[[Category: Aspartate-semialdehyde dehydrogenase]]		[[Category: Aspartate-semialdehyde dehydrogenase]]
-	[[Category: Burkholderia thailandensis]]	+	[[Category: Burkholderia thailandensis e264]]
	[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]		[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]
	[[Category: Dehydrogenase]]		[[Category: Dehydrogenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 06:49, 20 October 2012

2012-10-20T06:49:53Z

←Older revision		Revision as of 06:49, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3uw3]] is a 2 chain structure ~~of [[Aldehyde dehydrogenase]] and [[Aspartate-semialdehyde dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Burkholderia_thailandensis Burkholderia thailandensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA].	+	[[3uw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_thailandensis Burkholderia thailandensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA].

	==See Also==		==See Also==

OCA at 14:25, 25 July 2012

2012-07-25T14:25:48Z

←Older revision		Revision as of 14:25, 25 July 2012
Line 1:		Line 1:
-	[[Image:3uw3.~~jpg~~\|left\|200px]]	+	[[Image:3uw3.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3uw3", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3uw3\| PDB=3uw3 \| SCENE= }}		{{STRUCTURE_3uw3\| PDB=3uw3 \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3uw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_thailandensis Burkholderia thailandensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA].	+	[[3uw3]] is a 2 chain structure of [[Aldehyde dehydrogenase]] and [[Aspartate-semialdehyde dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Burkholderia_thailandensis Burkholderia thailandensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA].
		+
		+	==See Also==
		+	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]
		+	*[[Aspartate-semialdehyde dehydrogenase\|Aspartate-semialdehyde dehydrogenase]]
	[[Category: Aspartate-semialdehyde dehydrogenase]]		[[Category: Aspartate-semialdehyde dehydrogenase]]
	[[Category: Burkholderia thailandensis]]		[[Category: Burkholderia thailandensis]]

OCA at 11:59, 14 December 2011

2011-12-14T11:59:40Z

←Older revision		Revision as of 11:59, 14 December 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3uw3.jpg\|left\|200px]]

-	The ~~entry 3uw3~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3uw3", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3uw3\| PDB=3uw3 \| SCENE= }}

-	~~Authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID)~~	+	===Crystal Structure of an Aspartate-Semialdehyde Dehydrogenase from Burkholderia Thailandensis===

-	~~Description: Crystal~~ Structure ~~of an~~ Aspartate-~~Semialdehyde Dehydrogenase from~~ Burkholderia ~~Thailandensis~~	+
		+	==About this Structure==
		+	[[3uw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_thailandensis Burkholderia thailandensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UW3 OCA].
		+	[[Category: Aspartate-semialdehyde dehydrogenase]]
		+	[[Category: Burkholderia thailandensis]]
		+	[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]
		+	[[Category: Dehydrogenase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Structural genomic]]

OCA: Protected "3uw3" [edit=sysop:move=sysop]

2011-12-07T07:41:09Z

Protected "3uw3" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:41, 7 December 2011