3v2u - Revision history

OCA at 14:15, 14 March 2024

2024-03-14T14:15:42Z

←Older revision		Revision as of 14:15, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3v2u' size='340' side='right'caption='[[3v2u]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3v2u' size='340' side='right'caption='[[3v2u]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v2u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v2u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.105Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bts\|3bts]], [[3btu\|3btu]], [[3btv\|3btv]], [[2aj4\|2aj4]], [[3v5r\|3v5r]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL80, YML051W, YM9958.12, YM9827.01 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GAL3, YDR009W, YD8119.14 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2u OCA], [https://pdbe.org/3v2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v2u RCSB], [https://www.ebi.ac.uk/pdbsum/3v2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v2u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2u OCA], [https://pdbe.org/3v2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v2u RCSB], [https://www.ebi.ac.uk/pdbsum/3v2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v2u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GAL80_YEAST GAL80_YEAST]] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar. [[https://www.uniprot.org/uniprot/GAL3_YEAST GAL3_YEAST]] The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.	+	[https://www.uniprot.org/uniprot/GAL80_YEAST GAL80_YEAST] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	A wealth of genetic information and some biochemical analysis have made the GAL regulon of the yeast Saccharomyces cerevisiae a classic model system for studying transcriptional activation in eukaryotes. Galactose induces this transcriptional switch, which is regulated by three proteins: the transcriptional activator Gal4p, bound to DNA; the repressor Gal80p; and the transducer Gal3p. We showed previously that NADP appears to act as a trigger to kick the repressor off the activator. Sustained activation involves a complex of the transducer Gal3p and Gal80p mediated by galactose and ATP. We solved the crystal structure of the complex of Gal3p-Gal80p with alpha-D-galactose and ATP to 2.1 A resolution. The interaction between the proteins occurs only when Gal3p is in a "closed" state induced by ligand binding. The structure of the complex provides a rationale for the phenotypes of several well-known Gal80p and Gal3p mutants as well as the lack of galactokinase activity of Gal3p.	+
-		+
-	The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.,Lavy T, Kumar PR, He H, Joshua-Tor L Genes Dev. 2012 Feb 1;26(3):294-303. doi: 10.1101/gad.182691.111. PMID:22302941<ref>PMID:22302941</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3v2u" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Gal3-Gal80-Gal4\|Gal3-Gal80-Gal4]]		*[[Gal3-Gal80-Gal4\|Gal3-Gal80-Gal4]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: He, H]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Joshua-Tor, L]]	+	[[Category: He H]]
-	[[Category: Kumar~~, P R~~]]	+	[[Category: Joshua-Tor L]]
-	[[Category: Lavy, T]]	+	[[Category: Kumar PR]]
-	~~[[Category: Ghmp superfamily]]~~	+	[[Category: Lavy T]]
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation~~]]	+

OCA at 08:25, 20 July 2022

2022-07-20T08:25:59Z

←Older revision		Revision as of 08:25, 20 July 2022
Line 1:		Line 1:

	==Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP==		==Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP==
-	<StructureSection load='3v2u' size='340' side='right' caption='[[3v2u]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3v2u' size='340' side='right'caption='[[3v2u]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v2u]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3V2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v2u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bts\|3bts]], [[3btu\|3btu]], [[3btv\|3btv]], [[2aj4\|2aj4]], [[3v5r\|3v5r]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bts\|3bts]], [[3btu\|3btu]], [[3btv\|3btv]], [[2aj4\|2aj4]], [[3v5r\|3v5r]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL80, YML051W, YM9958.12, YM9827.01 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GAL3, YDR009W, YD8119.14 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL80, YML051W, YM9958.12, YM9827.01 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GAL3, YDR009W, YD8119.14 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3v2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2u OCA], [~~http~~://pdbe.org/3v2u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3v2u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3v2u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3v2u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2u OCA], [https://pdbe.org/3v2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v2u RCSB], [https://www.ebi.ac.uk/pdbsum/3v2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v2u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GAL80_YEAST GAL80_YEAST]] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar. [[~~http~~://www.uniprot.org/uniprot/GAL3_YEAST GAL3_YEAST]] The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.	+	[[https://www.uniprot.org/uniprot/GAL80_YEAST GAL80_YEAST]] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar. [[https://www.uniprot.org/uniprot/GAL3_YEAST GAL3_YEAST]] The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: He, H]]		[[Category: He, H]]
	[[Category: Joshua-Tor, L]]		[[Category: Joshua-Tor, L]]

OCA at 12:33, 4 August 2016

2016-08-04T12:33:47Z

←Older revision		Revision as of 12:33, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP==		==Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP==
	<StructureSection load='3v2u' size='340' side='right' caption='[[3v2u]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3v2u' size='340' side='right' caption='[[3v2u]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V2U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bts\|3bts]], [[3btu\|3btu]], [[3btv\|3btv]], [[2aj4\|2aj4]], [[3v5r\|3v5r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bts\|3bts]], [[3btu\|3btu]], [[3btv\|3btv]], [[2aj4\|2aj4]], [[3v5r\|3v5r]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL80, YML051W, YM9958.12, YM9827.01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~4932 Saccharomyces cerevisiae~~]), GAL3, YDR009W, YD8119.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~4932 Saccharomyces cerevisiae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL80, YML051W, YM9958.12, YM9827.01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GAL3, YDR009W, YD8119.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v2u RCSB], [http://www.ebi.ac.uk/pdbsum/3v2u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2u OCA], [http://pdbe.org/3v2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v2u RCSB], [http://www.ebi.ac.uk/pdbsum/3v2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v2u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/GAL80_YEAST GAL80_YEAST]] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar.	+	[[http://www.uniprot.org/uniprot/GAL80_YEAST GAL80_YEAST]] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar. [[http://www.uniprot.org/uniprot/GAL3_YEAST GAL3_YEAST]] The GAL3 regulatory function is required for rapid induction of the galactose system. At normal induction, galactose in the presence of the GAL3 protein may lead to the induction of the GAL genes, including GAL1. Then the galactokinase protein (GAL1) in the presence of galactose may reinforce the induction leading to a higher expression level. Upon depletion of galactose, the inducing activity of the galactokinase protein may decrease, after which transcription of the GAL genes, including GAL1, may decrease.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3v2u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Baker's yeast]]
	[[Category: He, H]]		[[Category: He, H]]
	[[Category: Joshua-Tor, L]]		[[Category: Joshua-Tor, L]]

OCA at 14:32, 24 December 2014

2014-12-24T14:32:53Z

←Older revision		Revision as of 14:32, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3v2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V2U FirstGlance]. <br>		<table><tr><td colspan='2'>[[3v2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V2U FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bts\|3bts]], [[3btu\|3btu]], [[3btv\|3btv]], [[2aj4\|2aj4]], [[3v5r\|3v5r]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bts\|3bts]], [[3btu\|3btu]], [[3btv\|3btv]], [[2aj4\|2aj4]], [[3v5r\|3v5r]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL80, YML051W, YM9958.12, YM9827.01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), GAL3, YDR009W, YD8119.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL80, YML051W, YM9958.12, YM9827.01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), GAL3, YDR009W, YD8119.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v2u RCSB], [http://www.ebi.ac.uk/pdbsum/3v2u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v2u RCSB], [http://www.ebi.ac.uk/pdbsum/3v2u PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GAL80_YEAST GAL80_YEAST]] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.,Lavy T, Kumar PR, He H, Joshua-Tor L Genes Dev. 2012 Feb 1;26(3):294-303. doi: 10.1101/gad.182691.111. PMID:22302941<ref>PMID:22302941</ref>		The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.,Lavy T, Kumar PR, He H, Joshua-Tor L Genes Dev. 2012 Feb 1;26(3):294-303. doi: 10.1101/gad.182691.111. PMID:22302941<ref>PMID:22302941</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: He, H.]]	+	[[Category: He, H]]
-	[[Category: Joshua-Tor, L.]]	+	[[Category: Joshua-Tor, L]]
-	[[Category: Kumar, P R.]]	+	[[Category: Kumar, P R]]
-	[[Category: Lavy, T.]]	+	[[Category: Lavy, T]]
	[[Category: Ghmp superfamily]]		[[Category: Ghmp superfamily]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

OCA at 06:09, 5 June 2014

2014-06-05T06:09:44Z

←Older revision		Revision as of 06:09, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3v2u.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP==
		+	<StructureSection load='3v2u' size='340' side='right' caption='[[3v2u]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3v2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V2U FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bts\|3bts]], [[3btu\|3btu]], [[3btv\|3btv]], [[2aj4\|2aj4]], [[3v5r\|3v5r]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL80, YML051W, YM9958.12, YM9827.01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), GAL3, YDR009W, YD8119.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v2u RCSB], [http://www.ebi.ac.uk/pdbsum/3v2u PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A wealth of genetic information and some biochemical analysis have made the GAL regulon of the yeast Saccharomyces cerevisiae a classic model system for studying transcriptional activation in eukaryotes. Galactose induces this transcriptional switch, which is regulated by three proteins: the transcriptional activator Gal4p, bound to DNA; the repressor Gal80p; and the transducer Gal3p. We showed previously that NADP appears to act as a trigger to kick the repressor off the activator. Sustained activation involves a complex of the transducer Gal3p and Gal80p mediated by galactose and ATP. We solved the crystal structure of the complex of Gal3p-Gal80p with alpha-D-galactose and ATP to 2.1 A resolution. The interaction between the proteins occurs only when Gal3p is in a "closed" state induced by ligand binding. The structure of the complex provides a rationale for the phenotypes of several well-known Gal80p and Gal3p mutants as well as the lack of galactokinase activity of Gal3p.

-	~~<!--~~	+	The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.,Lavy T, Kumar PR, He H, Joshua-Tor L Genes Dev. 2012 Feb 1;26(3):294-303. doi: 10.1101/gad.182691.111. PMID:22302941<ref>PMID:22302941</ref>
-	The ~~line below this paragraph, containing "STRUCTURE_3v2u", creates~~ the ~~"Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3v2u\| PDB=3v2u \| SCENE= }}~~	+

-	~~===Crystal structure~~ of the ~~yeast GAL regulon complex~~ of ~~the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Gal3-Gal80-Gal4\|Gal3-Gal80-Gal4]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22302941}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 22302941 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_22302941}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~3v2u~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022302941<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: He, H.]]		[[Category: He, H.]]

OCA at 06:44, 21 March 2012

2012-03-21T06:44:10Z

←Older revision		Revision as of 06:44, 21 March 2012
Line 11:		Line 11:
	===Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP===		===Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22302941}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22302941 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22302941}}

	==About this Structure==		==About this Structure==
	[[3v2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA].		[[3v2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022302941</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: He, H.]]		[[Category: He, H.]]

OCA at 05:46, 14 March 2012

2012-03-14T05:46:18Z

←Older revision		Revision as of 05:46, 14 March 2012
Line 1:		Line 1:
-	[[Image:3v2u.~~jpg~~\|left\|200px]]	+	[[Image:3v2u.png\|left\|200px]]

	<!--		<!--

OCA at 06:42, 8 February 2012

2012-02-08T06:42:48Z

←Older revision		Revision as of 06:42, 8 February 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3v2u.jpg\|left\|200px]]

-	The ~~entry 3v2u~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3v2u", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3v2u\| PDB=3v2u \| SCENE= }}

-	~~Authors: Lavy~~, T., ~~Kumar~~, ~~P.R.~~, ~~He, H., Joshua-Tor, L.~~	+	===Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP===

-	~~Description~~:	+
		+	==About this Structure==
		+	[[3v2u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V2U OCA].
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: He, H.]]
		+	[[Category: Joshua-Tor, L.]]
		+	[[Category: Kumar, P R.]]
		+	[[Category: Lavy, T.]]
		+	[[Category: Ghmp superfamily]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription regulation]]

OCA at 10:17, 18 January 2012

2012-01-18T10:17:14Z

←Older revision		Revision as of 10:17, 18 January 2012
Line 5:		Line 5:
	Authors: Lavy, T., Kumar, P.R., He, H., Joshua-Tor, L.		Authors: Lavy, T., Kumar, P.R., He, H., Joshua-Tor, L.

-	Description: ~~Transcription Complex~~	+	Description:

OCA at 05:57, 11 January 2012

2012-01-11T05:57:58Z

←Older revision		Revision as of 05:57, 11 January 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v2u is ON HOLD	+	The entry 3v2u is ON HOLD until Paper Publication

	Authors: Lavy, T., Kumar, P.R., He, H., Joshua-Tor, L.		Authors: Lavy, T., Kumar, P.R., He, H., Joshua-Tor, L.

	Description: Transcription Complex		Description: Transcription Complex