3v7z - Revision history

OCA at 08:38, 20 July 2022

2022-07-20T08:38:56Z

←Older revision		Revision as of 08:38, 20 July 2022
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		+
	==Carboxypeptidase T with GEMSA==		==Carboxypeptidase T with GEMSA==
-	<StructureSection load='3v7z' size='340' side='right' caption='[[3v7z]], [[Resolution\|resolution]] 1.61Å' scene=''>	+	<StructureSection load='3v7z' size='340' side='right'caption='[[3v7z]], [[Resolution\|resolution]] 1.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v7z]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43649 Atcc 43649]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V7Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3V7Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v7z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43649 Atcc 43649]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V7Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GEM:(2-GUANIDINOETHYLMERCAPTO)SUCCINIC+ACID'>GEM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GEM:(2-GUANIDINOETHYLMERCAPTO)SUCCINIC+ACID'>GEM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cpt ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2026 ATCC 43649])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cpt ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2026 ATCC 43649])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Carboxypeptidase_T Carboxypeptidase T], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.18 3.4.17.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Carboxypeptidase_T Carboxypeptidase T], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.18 3.4.17.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3v7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v7z OCA], [~~http~~://pdbe.org/3v7z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3v7z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3v7z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v7z OCA], [https://pdbe.org/3v7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v7z RCSB], [https://www.ebi.ac.uk/pdbsum/3v7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v7z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CBPT_THEVU CBPT_THEVU]] Able to split off hydrophobic and basic amino acids with comparable efficiency.	+	[[https://www.uniprot.org/uniprot/CBPT_THEVU CBPT_THEVU]] Able to split off hydrophobic and basic amino acids with comparable efficiency.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:

	==See Also==		==See Also==
-	*[[Carboxypeptidase\|Carboxypeptidase]]	+	*[[Carboxypeptidase 3D structures\|Carboxypeptidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 29:
	[[Category: Atcc 43649]]		[[Category: Atcc 43649]]
	[[Category: Carboxypeptidase T]]		[[Category: Carboxypeptidase T]]
		+	[[Category: Large Structures]]
	[[Category: Akparov, V K]]		[[Category: Akparov, V K]]
	[[Category: Kuranova, I P]]		[[Category: Kuranova, I P]]

OCA at 08:07, 30 September 2015

2015-09-30T08:07:00Z

←Older revision		Revision as of 08:07, 30 September 2015
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cpt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2026 ATCC 43649])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cpt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2026 ATCC 43649])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_T Carboxypeptidase T], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.18 3.4.17.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_T Carboxypeptidase T], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.18 3.4.17.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v7z RCSB], [http://www.ebi.ac.uk/pdbsum/3v7z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v7z OCA], [http://pdbe.org/3v7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v7z RCSB], [http://www.ebi.ac.uk/pdbsum/3v7z PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CBPT_THEVU CBPT_THEVU]] Able to split off hydrophobic and basic amino acids with comparable efficiency.		[[http://www.uniprot.org/uniprot/CBPT_THEVU CBPT_THEVU]] Able to split off hydrophobic and basic amino acids with comparable efficiency.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structures of carboxypeptidase T (CpT) complexes with phenylalanine and arginine substrate analogs - benzylsuccinic acid and (2-guanidinoethylmercapto)succinic acid - were determined by the molecular replacement method at resolutions of 1.57 A and 1.62 A to clarify the broad substrate specificity profile of the enzyme. The conservative Leu211 and Leu254 residues (also present in both carboxypeptidase A and carboxypeptidase B) were shown to be structural determinants for recognition of hydrophobic substrates, whereas Asp263 was for recognition of positively charged substrates. Mutations of these determinants modify the substrate profile: the CpT variant Leu211Gln acquires carboxypeptidase B-like properties, and the CpT variant Asp263Asn the carboxypeptidase A-like selectivity. The Pro248-Asp258 loop interacting with Leu254 and Tyr255 was shown to be responsible for recognition of the substrate's C-terminal residue. Substrate binding at the S1' subsite leads to the ligand-dependent shift of this loop, and Leu254 side chain movement induces the conformation rearrangement of the Glu277 residue crucial for catalysis. This is a novel insight into the substrate selectivity of metallocarboxypeptidases that demonstrates the importance of interactions between the S1' subsite and the catalytic center.
		+
		+	Structural insights into the broad substrate specificity of carboxypeptidase T from Thermoactinomyces vulgaris.,Akparov VKh, Timofeev VI, Khaliullin IG, Svedas V, Chestukhina GG, Kuranova IP FEBS J. 2015 Apr;282(7):1214-24. doi: 10.1111/febs.13210. Epub 2015 Feb 18. PMID:25619204<ref>PMID:25619204</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3v7z" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Carboxypeptidase\|Carboxypeptidase]]		*[[Carboxypeptidase\|Carboxypeptidase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 18:30, 25 December 2014

2014-12-25T18:30:30Z

←Older revision		Revision as of 18:30, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v7z RCSB], [http://www.ebi.ac.uk/pdbsum/3v7z PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v7z RCSB], [http://www.ebi.ac.uk/pdbsum/3v7z PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CBPT_THEVU CBPT_THEVU]] Able to split off hydrophobic and basic amino acids with comparable efficiency.

	==See Also==		==See Also==

OCA at 16:12, 9 December 2014

2014-12-09T16:12:29Z

←Older revision		Revision as of 16:12, 9 December 2014
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-	'''~~Unreleased~~ structure'''	+	==Carboxypeptidase T with GEMSA==
		+	<StructureSection load='3v7z' size='340' side='right' caption='[[3v7z]], [[Resolution\|resolution]] 1.61Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3v7z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43649 Atcc 43649]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V7Z FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GEM:(2-GUANIDINOETHYLMERCAPTO)SUCCINIC+ACID'>GEM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cpt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2026 ATCC 43649])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_T Carboxypeptidase T], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.18 3.4.17.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v7z RCSB], [http://www.ebi.ac.uk/pdbsum/3v7z PDBsum]</span></td></tr>
		+	</table>

-	~~The entry 3v7z is ON HOLD until Dec 24 2013~~	+	==See Also==
-		+	*[[Carboxypeptidase\|Carboxypeptidase]]
-	~~Authors~~: ~~Kuznetsov, S.A., Timofeev, V.I.,~~ Akparov, V.K., Kuranova, I.P.	+	__TOC__
-		+	</StructureSection>
-	~~Description~~: ~~Carboxypeptidase T with GEMSA~~	+	[[Category: Atcc 43649]]
		+	[[Category: Carboxypeptidase T]]
		+	[[Category: Akparov, V K]]
		+	[[Category: Kuranova, I P]]
		+	[[Category: Kuznetsov, S A]]
		+	[[Category: Timofeev, V I]]
		+	[[Category: Hydrolase]]
		+	[[Category: Peptidase]]

OCA at 13:08, 8 November 2012

2012-11-08T13:08:05Z

←Older revision		Revision as of 13:08, 8 November 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v7z is ON HOLD until ~~Paper Publication~~	+	The entry 3v7z is ON HOLD until Dec 24 2013

	Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P.		Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P.

	Description: Carboxypeptidase T with GEMSA		Description: Carboxypeptidase T with GEMSA

OCA at 06:30, 11 April 2012

2012-04-11T06:30:22Z

←Older revision		Revision as of 06:30, 11 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v7z is ON HOLD	+	The entry 3v7z is ON HOLD until Paper Publication

	Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P.		Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P.

	Description: Carboxypeptidase T with GEMSA		Description: Carboxypeptidase T with GEMSA

OCA at 07:21, 22 February 2012

2012-02-22T07:21:22Z

←Older revision		Revision as of 07:21, 22 February 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v7z is ON HOLD ~~until Paper Publication~~	+	The entry 3v7z is ON HOLD

	Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P.		Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P.

	Description: Carboxypeptidase T with GEMSA		Description: Carboxypeptidase T with GEMSA

OCA at 06:02, 25 January 2012

2012-01-25T06:02:34Z

←Older revision		Revision as of 06:02, 25 January 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v7z is ON HOLD	+	The entry 3v7z is ON HOLD until Paper Publication

	Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P.		Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P.

	Description: Carboxypeptidase T with GEMSA		Description: Carboxypeptidase T with GEMSA

OCA: Protected "3v7z" [edit=sysop:move=sysop]

2011-12-28T06:54:14Z

Protected "3v7z" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:54, 28 December 2011

OCA: New page: '''Unreleased structure''' The entry 3v7z is ON HOLD Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P. Description: Carboxypeptidase T with GEMSA

2011-12-28T06:54:13Z

New page: '''Unreleased structure''' The entry 3v7z is ON HOLD Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P. Description: Carboxypeptidase T with GEMSA

New page

'''Unreleased structure'''

The entry 3v7z is ON HOLD

Authors: Kuznetsov, S.A., Timofeev, V.I., Akparov, V.K., Kuranova, I.P.

Description: Carboxypeptidase T with GEMSA