3vac - Revision history

OCA at 18:35, 27 July 2022

2022-07-27T18:35:14Z

←Older revision		Revision as of 18:35, 27 July 2022
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	==Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D==		==Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D==
-	<StructureSection load='3vac' size='340' side='right' caption='[[3vac]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='3vac' size='340' side='right'caption='[[3vac]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vac]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VAC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VAC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vac]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VAC FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hb0\|2hb0]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hb0\|2hb0]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cfaE ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cfaE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vac OCA], [~~http~~://pdbe.org/3vac PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vac RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vac PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vac ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vac OCA], [https://pdbe.org/3vac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vac RCSB], [https://www.ebi.ac.uk/pdbsum/3vac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vac ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 3vac" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3vac" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Colonization factor antigen\|Colonization factor antigen]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Esser, L]]		[[Category: Esser, L]]
	[[Category: Liu, Y]]		[[Category: Liu, Y]]

OCA at 10:14, 8 November 2017

2017-11-08T10:14:43Z

←Older revision		Revision as of 10:14, 8 November 2017
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 3vac" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3vac" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Colonization factor antigen\|Colonization factor antigen]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:25, 4 August 2016

2016-08-04T13:25:02Z

←Older revision		Revision as of 13:25, 4 August 2016
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		+
	==Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D==		==Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D==
	<StructureSection load='3vac' size='340' side='right' caption='[[3vac]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3vac' size='340' side='right' caption='[[3vac]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vac]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VAC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vac]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VAC FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hb0\|2hb0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hb0\|2hb0]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cfaE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cfaE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vac OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vac RCSB], [http://www.ebi.ac.uk/pdbsum/3vac PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vac OCA], [http://pdbe.org/3vac PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vac RCSB], [http://www.ebi.ac.uk/pdbsum/3vac PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vac ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vac" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Esser, L]]		[[Category: Esser, L]]
	[[Category: Liu, Y]]		[[Category: Liu, Y]]

OCA at 06:36, 21 December 2014

2014-12-21T06:36:26Z

←Older revision		Revision as of 06:36, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3vac\| PDB=3vac \| SCENE= }}~~	+	==Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D==
-	===Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D===	+	<StructureSection load='3vac' size='340' side='right' caption='[[3vac]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23393133}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3vac]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VAC FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hb0\|2hb0]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cfaE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vac OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vac RCSB], [http://www.ebi.ac.uk/pdbsum/3vac PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CfaE, the tip adhesin of enterotoxigenic Escherichia coli colonization factor antigen I fimbriae, initiates binding of this enteropathogen to the small intestine. It comprises stacked beta-sandwich adhesin and pilin domains with the putative receptor-binding pocket at one pole and an equatorial interdomain interface. CfaE binding to erythrocytes is enhanced by application of moderate shear stress. A G168D replacement along the adhesin domain facing of the CfaE interdomain region was previously shown to decrease the dependence on shear by increasing binding at lower shear forces. To elucidate the structural basis for this functional change, we studied the properties of CfaE/G168D (with self-complemented donor strand), and solved its crystal structure at 2.6 A resolution. Compared to native CfaE, CfaE/G168D showed a downward shift in peak erythrocyte binding under shear stress and greater binding under static conditions. The thermal melting transition of CfaE/G168D occurred 10 degrees C below that of CfaE. Compared to CfaE, the atomic structure of CfaE/G168D revealed a 36% reduction in the buried surface area at the interdomain interface. Despite location of this single modification in the adhesin domain, CfaE/G168D exhibited structural derangements only in the adjoining pilin domain when compared to CfaE. In molecular dynamics simulations, the G168D mutation was associated with weakened interdomain interactions under tensile force. Taken together, these findings indicate that the adhesin and pilin domains of CfaE are conformationally tightly coupled and support the hypothesis that opening of the interface plays a critical modulatory role in the allosteric activation of CfaE.

-	~~==About this Structure==~~	+	Tight Conformational Coupling between the Domains of the Enterotoxigenic Escherichia coli Fimbrial Adhesin CfaE Regulates Binding State Transition.,Liu Y, Esser L, Interlandi G, Kisiela DI, Tchesnokova V, Thomas WE, Sokurenko E, Xia D, Savarino SJ J Biol Chem. 2013 Feb 7. PMID:23393133<ref>PMID:23393133</ref>
-	~~[[3vac]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli~~ Escherichia coli]. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3VAC OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Colonization factor antigen\|Colonization factor antigen]]		*[[Colonization factor antigen\|Colonization factor antigen]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Esser, L.]]	+	[[Category: Esser, L]]
-	[[Category: Liu, Y.]]	+	[[Category: Liu, Y]]
-	[[Category: Xia, D.]]	+	[[Category: Xia, D]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Cfa/i etec adhesin]]		[[Category: Cfa/i etec adhesin]]
	[[Category: Ig fold]]		[[Category: Ig fold]]

OCA at 10:13, 24 April 2013

2013-04-24T10:13:03Z

←Older revision		Revision as of 10:13, 24 April 2013
Line 1:		Line 1:
-	[[Image:3vac.jpg\|left\|200px]]
-
	{{STRUCTURE_3vac\| PDB=3vac \| SCENE= }}		{{STRUCTURE_3vac\| PDB=3vac \| SCENE= }}
-
	===Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D===		===Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D===
-
	{{ABSTRACT_PUBMED_23393133}}		{{ABSTRACT_PUBMED_23393133}}

	==About this Structure==		==About this Structure==
	[[3vac]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VAC OCA].		[[3vac]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VAC OCA].
		+
		+	==See Also==
		+	*[[Colonization factor antigen\|Colonization factor antigen]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Esser, L.]]		[[Category: Esser, L.]]

OCA at 13:50, 20 February 2013

2013-02-20T13:50:37Z

←Older revision		Revision as of 13:50, 20 February 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3vac.jpg\|left\|200px]]

-	~~The entry~~ 3vac ~~is ON HOLD~~ ~~until Apr 03 2014~~	+	{{STRUCTURE_3vac\| PDB=3vac \| SCENE= }}

-	~~Authors: Liu, Y., Esser, L., Xia, D~~.	+	===Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D===

-	~~Description: Crystal~~ Structure ~~of the CFA~~/~~I Enterotoxigenic E~~. coli adhesin ~~CfaE mutant G168D~~	+	{{ABSTRACT_PUBMED_23393133}}
		+
		+	==About this Structure==
		+	[[3vac]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VAC OCA].
		+	[[Category: Escherichia coli]]
		+	[[Category: Esser, L.]]
		+	[[Category: Liu, Y.]]
		+	[[Category: Xia, D.]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Cfa/i etec adhesin]]
		+	[[Category: Ig fold]]

OCA at 10:45, 19 December 2012

2012-12-19T10:45:31Z

←Older revision		Revision as of 10:45, 19 December 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vac is ON HOLD until ~~Paper Publication~~	+	The entry 3vac is ON HOLD until Apr 03 2014

	Authors: Liu, Y., Esser, L., Xia, D.		Authors: Liu, Y., Esser, L., Xia, D.

	Description: Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D		Description: Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D

OCA at 06:52, 1 February 2012

2012-02-01T06:52:07Z

←Older revision		Revision as of 06:52, 1 February 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vac is ON HOLD	+	The entry 3vac is ON HOLD until Paper Publication

	Authors: Liu, Y., Esser, L., Xia, D.		Authors: Liu, Y., Esser, L., Xia, D.

	Description: Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D		Description: Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D

OCA at 05:59, 11 January 2012

2012-01-11T05:59:42Z

←Older revision		Revision as of 05:59, 11 January 2012
Line 5:		Line 5:
	Authors: Liu, Y., Esser, L., Xia, D.		Authors: Liu, Y., Esser, L., Xia, D.

-	Description: Crystal Structure of the CFA/I Enterotoxigenic e. coli. adhesin CfaE mutant G168D	+	Description: Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D

OCA: Protected "3vac" [edit=sysop:move=sysop]

2012-01-04T05:48:06Z

Protected "3vac" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:48, 4 January 2012