3vpt - Revision history

OCA at 08:41, 20 March 2024

2024-03-20T08:41:18Z

←Older revision		Revision as of 08:41, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3vpt' size='340' side='right'caption='[[3vpt]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3vpt' size='340' side='right'caption='[[3vpt]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bommo Bommo~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VPT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGO:S~~-1,~~2-PROPANEDIOL'>PGO</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3vpq\|3vpq]], [[3vpu\|3vpu]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~GST sigma ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=7091 BOMMO]~~)~~</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=2.5.~~1~~.18~~ 2~~.5.1.18]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpt OCA], [https://pdbe.org/3vpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vpt RCSB], [https://www.ebi.ac.uk/pdbsum/3vpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vpt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpt OCA], [https://pdbe.org/3vpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vpt RCSB], [https://www.ebi.ac.uk/pdbsum/3vpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vpt ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q5CCJ4_BOMMO Q5CCJ4_BOMMO]
-	~~BACKGROUND~~: Glutathione transferases (GSTs) are members of a major family of detoxification enzymes. Here, we report the crystal structure of a sigma-class GST of Bombyx mori, bmGSTS1, to gain insight into the mechanism catalysis. METHODS: The structure of bmGSTS1 and its complex with glutathione were determined at resolutions of 1.9A and 1.7A by synchrotron radiation and the molecular replacement method. RESULTS: The three-dimensional structure of bmGSTS1 shows that it exists as a dimer and is similar in structure to other GSTs with respect to its secondary and tertiary structures. Although striking similarities to the structure of prostaglandin D synthase were also detected, we were surprised to find that bmGSTS1 can convert prostaglandin H2 into its E2 form. Comparison of bmGSTS1 with its glutathione complex showed that bound glutathione was localized to the glutathione-binding site (G-site). Site-directed mutagenesis of bmGSTS1 mutants indicated that amino acid residues Tyr8, Leu14, Trp39, Lys43, Gln50, Met51, Gln63, and Ser64 in the G-site contribute to catalytic activity. CONCLUSION: We determined the tertiary structure of bmGSTS1 exhibiting prostaglandin E synthase activity. GENERAL SIGNIFICANCE: These results are, to our knowledge, the first report of a prostaglandin synthase activity in insects.	+
-		+
-	Crystal structure of a Bombyx mori sigma-class glutathione transferase exhibiting prostaglandin E synthase activity.,Yamamoto K, Higashiura A, Suzuki M, Aritake K, Urade Y, Uodome N, Nakagawa A Biochim Biophys Acta. 2013 Jun;1830(6):3711-8. doi: 10.1016/~~j.bbagen.2013.02.021., Epub 2013 Mar 1. PMID:23458683<ref>PMID:23458683<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3vpt" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]		*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bommo]]~~	+	[[Category: Bombyx mori]]
-	~~[[Category: Glutathione transferase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Higashiura, A]]	+	[[Category: Higashiura A]]
-	[[Category: Nakagawa, A]]	+	[[Category: Nakagawa A]]
-	[[Category: Suzuki, M]]	+	[[Category: Suzuki M]]
-	[[Category: Yamamoto, K]]	+	[[Category: Yamamoto K]]
-	~~[[Category: Cytosol]]~~	+
-	~~[[Category: Gsh binding]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 18:57, 27 July 2022

2022-07-27T18:57:39Z

←Older revision		Revision as of 18:57, 27 July 2022
Line 1:		Line 1:

	==Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form==		==Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form==
-	<StructureSection load='3vpt' size='340' side='right' caption='[[3vpt]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3vpt' size='340' side='right'caption='[[3vpt]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vpt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VPT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vpq\|3vpq]], [[3vpu\|3vpu]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3vpq\|3vpq]], [[3vpu\|3vpu]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GST sigma ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7091 BOMMO])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GST sigma ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7091 BOMMO])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpt OCA], [~~http~~://pdbe.org/3vpt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vpt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vpt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vpt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpt OCA], [https://pdbe.org/3vpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vpt RCSB], [https://www.ebi.ac.uk/pdbsum/3vpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vpt ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Glutathione S-transferase\|Glutathione S-transferase]]	+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	[[Category: Bommo]]		[[Category: Bommo]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
		+	[[Category: Large Structures]]
	[[Category: Higashiura, A]]		[[Category: Higashiura, A]]
	[[Category: Nakagawa, A]]		[[Category: Nakagawa, A]]

OCA at 12:21, 4 August 2016

2016-08-04T12:21:01Z

←Older revision		Revision as of 12:21, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form==		==Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form==
	<StructureSection load='3vpt' size='340' side='right' caption='[[3vpt]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3vpt' size='340' side='right' caption='[[3vpt]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vpt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bombyx_mori Bombyx mori~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VPT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vpt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VPT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vpq\|3vpq]], [[3vpu\|3vpu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vpq\|3vpq]], [[3vpu\|3vpu]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GST sigma ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7091 ~~Bombyx mori~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GST sigma ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7091 BOMMO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vpt RCSB], [http://www.ebi.ac.uk/pdbsum/3vpt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpt OCA], [http://pdbe.org/3vpt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vpt RCSB], [http://www.ebi.ac.uk/pdbsum/3vpt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vpt ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vpt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bombyx mori~~]]	+	[[Category: Bommo]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Higashiura, A]]		[[Category: Higashiura, A]]

OCA at 06:48, 21 December 2014

2014-12-21T06:48:17Z

←Older revision		Revision as of 06:48, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3vpt\| PDB=3vpt \| SCENE= }}~~	+	==Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form==
-	===Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form===	+	<StructureSection load='3vpt' size='340' side='right' caption='[[3vpt]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23458683}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3vpt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VPT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vpq\|3vpq]], [[3vpu\|3vpu]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GST sigma ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7091 Bombyx mori])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vpt RCSB], [http://www.ebi.ac.uk/pdbsum/3vpt PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Glutathione transferases (GSTs) are members of a major family of detoxification enzymes. Here, we report the crystal structure of a sigma-class GST of Bombyx mori, bmGSTS1, to gain insight into the mechanism catalysis. METHODS: The structure of bmGSTS1 and its complex with glutathione were determined at resolutions of 1.9A and 1.7A by synchrotron radiation and the molecular replacement method. RESULTS: The three-dimensional structure of bmGSTS1 shows that it exists as a dimer and is similar in structure to other GSTs with respect to its secondary and tertiary structures. Although striking similarities to the structure of prostaglandin D synthase were also detected, we were surprised to find that bmGSTS1 can convert prostaglandin H2 into its E2 form. Comparison of bmGSTS1 with its glutathione complex showed that bound glutathione was localized to the glutathione-binding site (G-site). Site-directed mutagenesis of bmGSTS1 mutants indicated that amino acid residues Tyr8, Leu14, Trp39, Lys43, Gln50, Met51, Gln63, and Ser64 in the G-site contribute to catalytic activity. CONCLUSION: We determined the tertiary structure of bmGSTS1 exhibiting prostaglandin E synthase activity. GENERAL SIGNIFICANCE: These results are, to our knowledge, the first report of a prostaglandin synthase activity in insects.

-	~~==About this Structure==~~	+	Crystal structure of a Bombyx mori sigma-class glutathione transferase exhibiting prostaglandin E synthase activity.,Yamamoto K, Higashiura A, Suzuki M, Aritake K, Urade Y, Uodome N, Nakagawa A Biochim Biophys Acta. 2013 Jun;1830(6):3711-8. doi: 10.1016/j.bbagen.2013.02.021., Epub 2013 Mar 1. PMID:23458683<ref>PMID:23458683</ref>
-	~~[[3vpt]] is a 1 chain~~ structure ~~with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori~~ Bombyx mori]. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.~~il/oca-bin/ocashort?id=3VPT OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:023458683</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase\|Glutathione S-transferase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bombyx mori]]		[[Category: Bombyx mori]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
-	[[Category: Higashiura, A.]]	+	[[Category: Higashiura, A]]
-	[[Category: Nakagawa, A.]]	+	[[Category: Nakagawa, A]]
-	[[Category: Suzuki, M.]]	+	[[Category: Suzuki, M]]
-	[[Category: Yamamoto, K.]]	+	[[Category: Yamamoto, K]]
	[[Category: Cytosol]]		[[Category: Cytosol]]
	[[Category: Gsh binding]]		[[Category: Gsh binding]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 03:49, 1 August 2013

2013-08-01T03:49:26Z

←Older revision		Revision as of 03:49, 1 August 2013
Line 1:		Line 1:
-	[[Image:3vpt.jpg\|left\|200px]]
-
	{{STRUCTURE_3vpt\| PDB=3vpt \| SCENE= }}		{{STRUCTURE_3vpt\| PDB=3vpt \| SCENE= }}
-
	===Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form===		===Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form===
-		+	{{ABSTRACT_PUBMED_23458683}}

	==About this Structure==		==About this Structure==
	[[3vpt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA].		[[3vpt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023458683</ref><references group="xtra"/><references/>
	[[Category: Bombyx mori]]		[[Category: Bombyx mori]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]

OCA at 14:19, 6 March 2013

2013-03-06T14:19:52Z

←Older revision		Revision as of 14:19, 6 March 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3vpt.jpg\|left\|200px]]

-	~~The entry~~ 3vpt ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3vpt\| PDB=3vpt \| SCENE= }}

-	~~Authors: Yamamoto, K., Higashiura, A., Suzuki, M., Nakagawa, A.~~	+	===Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form===

-	~~Description: Crystal~~ structure of Bombyx mori ~~sigma~~-~~class glutathione~~ transferase ~~in apo form~~	+
		+	==About this Structure==
		+	[[3vpt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPT OCA].
		+	[[Category: Bombyx mori]]
		+	[[Category: Glutathione transferase]]
		+	[[Category: Higashiura, A.]]
		+	[[Category: Nakagawa, A.]]
		+	[[Category: Suzuki, M.]]
		+	[[Category: Yamamoto, K.]]
		+	[[Category: Cytosol]]
		+	[[Category: Gsh binding]]
		+	[[Category: Transferase]]

OCA at 07:21, 18 April 2012

2012-04-18T07:21:58Z

←Older revision		Revision as of 07:21, 18 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vpt is ON HOLD	+	The entry 3vpt is ON HOLD until Paper Publication

	Authors: Yamamoto, K., Higashiura, A., Suzuki, M., Nakagawa, A.		Authors: Yamamoto, K., Higashiura, A., Suzuki, M., Nakagawa, A.

	Description: Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form		Description: Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form

OCA: Protected "3vpt" [edit=sysop:move=sysop]

2012-03-21T07:06:49Z

Protected "3vpt" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:06, 21 March 2012

OCA: New page: '''Unreleased structure''' The entry 3vpt is ON HOLD Authors: Yamamoto, K., Higashiura, A., Suzuki, M., Nakagawa, A. Description: Crystal structure of Bombyx mori sigma-class glutathio...

2012-03-21T07:06:48Z

New page: '''Unreleased structure''' The entry 3vpt is ON HOLD Authors: Yamamoto, K., Higashiura, A., Suzuki, M., Nakagawa, A. Description: Crystal structure of Bombyx mori sigma-class glutathio...

New page

'''Unreleased structure'''

The entry 3vpt is ON HOLD

Authors: Yamamoto, K., Higashiura, A., Suzuki, M., Nakagawa, A.

Description: Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form