3vuo - Revision history

OCA at 19:04, 27 July 2022

2022-07-27T19:04:57Z

←Older revision		Revision as of 19:04, 27 July 2022
Line 1:		Line 1:

	==Crystal structure of nontoxic nonhemagglutinin subcomponent (NTNHA) from clostridium botulinum serotype D strain 4947==		==Crystal structure of nontoxic nonhemagglutinin subcomponent (NTNHA) from clostridium botulinum serotype D strain 4947==
-	<StructureSection load='3vuo' size='340' side='right' caption='[[3vuo]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<StructureSection load='3vuo' size='340' side='right'caption='[[3vuo]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vuo]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VUO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vuo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VUO FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ntnha ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ntnha ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vuo OCA], [~~http~~://pdbe.org/3vuo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vuo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vuo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vuo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vuo OCA], [https://pdbe.org/3vuo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vuo RCSB], [https://www.ebi.ac.uk/pdbsum/3vuo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vuo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus botulinus van ermengem 1896]]		[[Category: Bacillus botulinus van ermengem 1896]]
		+	[[Category: Large Structures]]
	[[Category: Hasegawa, K]]		[[Category: Hasegawa, K]]
	[[Category: Hayashi, S]]		[[Category: Hayashi, S]]

OCA at 09:59, 11 August 2016

2016-08-11T09:59:56Z

←Older revision		Revision as of 09:59, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of nontoxic nonhemagglutinin subcomponent (NTNHA) from clostridium botulinum serotype D strain 4947==		==Crystal structure of nontoxic nonhemagglutinin subcomponent (NTNHA) from clostridium botulinum serotype D strain 4947==
	<StructureSection load='3vuo' size='340' side='right' caption='[[3vuo]], [[Resolution\|resolution]] 3.90Å' scene=''>		<StructureSection load='3vuo' size='340' side='right' caption='[[3vuo]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum Clostridium botulinum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VUO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VUO FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ntnha ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 ~~Clostridium botulinum~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ntnha ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vuo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vuo RCSB], [http://www.ebi.ac.uk/pdbsum/3vuo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vuo OCA], [http://pdbe.org/3vuo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vuo RCSB], [http://www.ebi.ac.uk/pdbsum/3vuo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vuo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vuo" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium botulinum~~]]	+	[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Hasegawa, K]]		[[Category: Hasegawa, K]]
	[[Category: Hayashi, S]]		[[Category: Hayashi, S]]

OCA at 10:24, 4 January 2015

2015-01-04T10:24:57Z

←Older revision		Revision as of 10:24, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3vuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VUO FirstGlance]. <br>		<table><tr><td colspan='2'>[[3vuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VUO FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ntnha ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ntnha ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vuo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vuo RCSB], [http://www.ebi.ac.uk/pdbsum/3vuo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vuo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vuo RCSB], [http://www.ebi.ac.uk/pdbsum/3vuo PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
-	[[Category: Hasegawa, K.]]	+	[[Category: Hasegawa, K]]
-	[[Category: Hayashi, S.]]	+	[[Category: Hayashi, S]]
-	[[Category: Inui, K.]]	+	[[Category: Inui, K]]
-	[[Category: Matsumoto, T.]]	+	[[Category: Matsumoto, T]]
-	[[Category: Miyashita, S I.]]	+	[[Category: Miyashita, S I]]
-	[[Category: Miyata, K.]]	+	[[Category: Miyata, K]]
-	[[Category: Niwa, K.]]	+	[[Category: Niwa, K]]
-	[[Category: Sagane, Y.]]	+	[[Category: Sagane, Y]]
-	[[Category: Suzuki, T.]]	+	[[Category: Suzuki, T]]
-	[[Category: Watanabe, T.]]	+	[[Category: Watanabe, T]]
-	[[Category: Yajima, S.]]	+	[[Category: Yajima, S]]
-	[[Category: Yamano, A.]]	+	[[Category: Yamano, A]]
	[[Category: Botulinum neurotoxin]]		[[Category: Botulinum neurotoxin]]
	[[Category: Protection of botulinum neurotoxin]]		[[Category: Protection of botulinum neurotoxin]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 06:55, 25 June 2014

2014-06-25T06:55:28Z

←Older revision		Revision as of 06:55, 25 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3vuo.~~jpg~~\|~~left\|200px~~]]	+	==Crystal structure of nontoxic nonhemagglutinin subcomponent (NTNHA) from clostridium botulinum serotype D strain 4947==
		+	<StructureSection load='3vuo' size='340' side='right' caption='[[3vuo]], [[Resolution\|resolution]] 3.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3vuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VUO FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ntnha ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vuo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vuo RCSB], [http://www.ebi.ac.uk/pdbsum/3vuo PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In cell culture supernatants, the botulinum neurotoxin (BoNT) exists as part of a toxin complex (TC) in which nontoxic nonhemagglutinin (NTNHA) and/or hemagglutinins (HAs) are assembled onto the BoNT. A series of investigations indicated that formation of the TC is vital for delivery of the toxin to nerve cells through the digestive tract. In the assembly process, BoNT binds to NTNHA yielding M-TC, and it then matures into L-TC by further association with the HAs via NTNHA in the M-TC. Here, we report a crystal structure of the NTNHA from Clostridium botulinum serotype D strain 4947. Additionally, we performed small-angle X-ray scattering (SAXS) analysis of the NTNHA and the M-TC to elucidate the solution structure. The crystal structure of D-4947 NTNHA revealed that BoNT and NTNHA share a closely related structure consisting of three domains. The SAXS image indicated that, even though the N-terminal two-thirds of the NTNHA molecule had an apparently similar conformation in both the crystal and solution structures, the C-terminal third of the molecule showed a more extended structure in the SAXS image than that seen in the crystallographic image. The discrepancy between the crystal and solution structures implies a high flexibility of the C-terminal third domain of NTNHA, which is involved in binding to BoNT. Structural dynamics of the NTNHA molecule revealed by SAXS may explain its binding to BoNT to form the BoNT/NTNHA complex.

-	~~{{STRUCTURE_3vuo\| PDB=3vuo \| SCENE= }}~~	+	Small-angle X-ray scattering reveals structural dynamics of the botulinum neurotoxin associating protein, nontoxic nonhemagglutinin.,Sagane Y, Miyashita S, Miyata K, Matsumoto T, Inui K, Hayashi S, Suzuki T, Hasegawa K, Yajima S, Yamano A, Niwa K, Watanabe T Biochem Biophys Res Commun. 2012 Aug 24;425(2):256-60. Epub 2012 Jul 22. PMID:22828508<ref>PMID:22828508</ref>

-	~~===Crystal structure~~ of ~~nontoxic nonhemagglutinin subcomponent (NTNHA) from clostridium botulinum serotype D strain 4947===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22828508}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3vuo]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUO OCA].~~	+	</StructureSection>
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Hasegawa, K.]]		[[Category: Hasegawa, K.]]

OCA at 07:16, 19 September 2012

2012-09-19T07:16:31Z

←Older revision		Revision as of 07:16, 19 September 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3vuo.jpg\|left\|200px]]

-	~~The entry~~ 3vuo ~~is ON HOLD~~	+	{{STRUCTURE_3vuo\| PDB=3vuo \| SCENE= }}

-	~~Authors: Sagane, Y., Miyashita, S.-I., Miyata, K., Matsumoto, T., Inui, K., Hayashi, S., Suzuki, T., Hasegawa, K., Yajima, S., Yamano, A., Niwa, K., Watanabe, T.~~	+	===Crystal structure of nontoxic nonhemagglutinin subcomponent (NTNHA) from clostridium botulinum serotype D strain 4947===

-	~~Description: Crystal~~ structure ~~of nontoxic nonhemagglutinin subcomponent (NTNHA)~~ from ~~clostridium~~ botulinum ~~serotype D strain 4947~~	+	{{ABSTRACT_PUBMED_22828508}}
		+
		+	==About this Structure==
		+	[[3vuo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUO OCA].
		+	[[Category: Clostridium botulinum]]
		+	[[Category: Hasegawa, K.]]
		+	[[Category: Hayashi, S.]]
		+	[[Category: Inui, K.]]
		+	[[Category: Matsumoto, T.]]
		+	[[Category: Miyashita, S I.]]
		+	[[Category: Miyata, K.]]
		+	[[Category: Niwa, K.]]
		+	[[Category: Sagane, Y.]]
		+	[[Category: Suzuki, T.]]
		+	[[Category: Watanabe, T.]]
		+	[[Category: Yajima, S.]]
		+	[[Category: Yamano, A.]]
		+	[[Category: Botulinum neurotoxin]]
		+	[[Category: Protection of botulinum neurotoxin]]
		+	[[Category: Toxin]]

OCA: Protected "3vuo" [edit=sysop:move=sysop]

2012-07-11T07:38:11Z

Protected "3vuo" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:38, 11 July 2012

OCA: New page: '''Unreleased structure''' The entry 3vuo is ON HOLD Authors: Sagane, Y., Miyashita, S.-I., Miyata, K., Matsumoto, T., Inui, K., Hayashi, S., Suzuki, T., Hasegawa, K., Yajima, S., Yaman...

2012-07-11T07:38:10Z

New page: '''Unreleased structure''' The entry 3vuo is ON HOLD Authors: Sagane, Y., Miyashita, S.-I., Miyata, K., Matsumoto, T., Inui, K., Hayashi, S., Suzuki, T., Hasegawa, K., Yajima, S., Yaman...

New page

'''Unreleased structure'''

The entry 3vuo is ON HOLD

Authors: Sagane, Y., Miyashita, S.-I., Miyata, K., Matsumoto, T., Inui, K., Hayashi, S., Suzuki, T., Hasegawa, K., Yajima, S., Yamano, A., Niwa, K., Watanabe, T.

Description: Crystal structure of nontoxic nonhemagglutinin subcomponent (NTNHA) from clostridium botulinum serotype D strain 4947