3we7 - Revision history

OCA at 05:33, 3 August 2022

2022-08-03T05:33:30Z

←Older revision		Revision as of 05:33, 3 August 2022
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	==Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii==		==Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii==
-	<StructureSection load='3we7' size='340' side='right' caption='[[3we7]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='3we7' size='340' side='right'caption='[[3we7]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3we7]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WE7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WE7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3we7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WE7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WE7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wl4\|3wl4]], [[3wl3\|3wl3]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH0499 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH0499 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [~~http~~://pdbe.org/3we7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3we7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3we7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [https://pdbe.org/3we7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [https://www.ebi.ac.uk/pdbsum/3we7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3we7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
	[[Category: Fukuda, Y]]		[[Category: Fukuda, Y]]

OCA at 09:40, 21 February 2019

2019-02-21T09:40:03Z

←Older revision		Revision as of 09:40, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='3we7' size='340' side='right' caption='[[3we7]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3we7' size='340' side='right' caption='[[3we7]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3we7]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WE7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3we7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WE7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH0499 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [http://pdbe.org/3we7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [http://www.ebi.ac.uk/pdbsum/3we7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3we7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [http://pdbe.org/3we7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [http://www.ebi.ac.uk/pdbsum/3we7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3we7 ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pyrococcus horikoshii]]
	[[Category: Fukuda, Y]]		[[Category: Fukuda, Y]]
	[[Category: Inoue, T]]		[[Category: Inoue, T]]

OCA at 15:44, 11 August 2016

2016-08-11T15:44:19Z

←Older revision		Revision as of 15:44, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii==		==Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii==
	<StructureSection load='3we7' size='340' side='right' caption='[[3we7]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3we7' size='340' side='right' caption='[[3we7]], [[Resolution\|resolution]] 1.55Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [http://www.ebi.ac.uk/pdbsum/3we7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [http://pdbe.org/3we7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [http://www.ebi.ac.uk/pdbsum/3we7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3we7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3we7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:56, 5 January 2015

2015-01-05T14:56:48Z

←Older revision		Revision as of 14:56, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3we7]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WE7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3we7]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WE7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [http://www.ebi.ac.uk/pdbsum/3we7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [http://www.ebi.ac.uk/pdbsum/3we7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fukuda, Y.]]	+	[[Category: Fukuda, Y]]
-	[[Category: Inoue, T.]]	+	[[Category: Inoue, T]]
-	[[Category: Mine, S.]]	+	[[Category: Mine, S]]
-	[[Category: Nakamura, T.]]	+	[[Category: Nakamura, T]]
-	[[Category: Sato, T.]]	+	[[Category: Sato, T]]
-	[[Category: Uegaki, K.]]	+	[[Category: Uegaki, K]]
	[[Category: Deacetylation]]		[[Category: Deacetylation]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Zinc binding]]		[[Category: Zinc binding]]

OCA at 13:30, 20 August 2014

2014-08-20T13:30:09Z

←Older revision		Revision as of 13:30, 20 August 2014
Line 2:		Line 2:
	<StructureSection load='3we7' size='340' side='right' caption='[[3we7]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3we7' size='340' side='right' caption='[[3we7]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3we7]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WE7 OCA]. <br>	+	<table><tr><td colspan='2'>[[3we7]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WE7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WE7 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [http://www.ebi.ac.uk/pdbsum/3we7 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [http://www.ebi.ac.uk/pdbsum/3we7 PDBsum]</span></td></tr>
	<table>		<table>
Line 14:		Line 13:
	Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N'-diacetylchitobiose deacetylase.,Mine S, Niiyama M, Hashimoto W, Ikegami T, Koma D, Ohmoto T, Fukuda Y, Inoue T, Abe Y, Ueda T, Morita J, Uegaki K, Nakamura T FEBS J. 2014 Apr 7. doi: 10.1111/febs.12805. PMID:24702737<ref>PMID:24702737</ref>		Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N'-diacetylchitobiose deacetylase.,Mine S, Niiyama M, Hashimoto W, Ikegami T, Koma D, Ohmoto T, Fukuda Y, Inoue T, Abe Y, Ueda T, Morita J, Uegaki K, Nakamura T FEBS J. 2014 Apr 7. doi: 10.1111/febs.12805. PMID:24702737<ref>PMID:24702737</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 08:14, 7 May 2014

2014-05-07T08:14:46Z

←Older revision		Revision as of 08:14, 7 May 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii==
		+	<StructureSection load='3we7' size='340' side='right' caption='[[3we7]], [[Resolution\|resolution]] 1.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3we7]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WE7 OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wl4\|3wl4]], [[3wl3\|3wl3]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3we7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3we7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3we7 RCSB], [http://www.ebi.ac.uk/pdbsum/3we7 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In order to develop a structure-based understanding of the chitinolytic pathway in hyperthermophilic Pyrococcus species, we performed crystallographic studies on N,N'-diacetylchitobiose deacetylases (Dacs) from Pyrococcus horikoshii (Ph-Dac) and Pyrococcus furiosus (Pf-Dac). Neither Ph-Dac nor Pf-Dac was expressed in the soluble fraction of Escherichia coli harboring the expression plasmid. However, insertion of the target genes into the chromosome of E. coli yielded the soluble recombinant protein. The purified Pyrococcus Dacs were active and thermostable up to 85 degrees C. The crystal structures of Ph-Dac and Pf-Dac were determined at resolutions of 2.0 A and 1.54 A, respectively. The Pyrococcus Dac forms a hexamer composed of two trimers. These Dacs are characterized by an intermolecular cleft, which is formed by two polypeptides in the trimeric assembly. In Ph-Dac, catalytic Zn situated at the end of the cleft is coordinated by three side chain ligands from His44, Asp47, and His155, and by a phosphate ion derived from the crystallization reservoir solution. We considered that the bound phosphate mimicked the tetrahedral oxyanion, which is an intermediate of hydrolysis of the N-acetyl group, and proposed an appropriate reaction mechanism. In the proposed mechanism, the Nepsilon atom of His264 (from the adjacent polypeptide in the Ph-Dac sequence) is directly involved in the stabilization of the oxyanion intermediate. Mutation analysis also indicated that His264 was essential to the catalysis. These factors give the archaeal Dacs an unprecedented active site architecture a Zn-dependent deacetylases. DATABASE: Structural data are available in the Protein Data Bank database under accession numbers 3WL3, 3WL4, and 3WE7. STRUCTURED DIGITAL ABSTRACT: Ph-Dac and Ph-Dac bind by x-ray crystallography (View interaction) Pf-Dac and Pf-Dac bind by x-ray crystallography (View interaction).

-	~~The entry 3we7 is ON HOLD until Paper Publication~~	+	Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N'-diacetylchitobiose deacetylase.,Mine S, Niiyama M, Hashimoto W, Ikegami T, Koma D, Ohmoto T, Fukuda Y, Inoue T, Abe Y, Ueda T, Morita J, Uegaki K, Nakamura T FEBS J. 2014 Apr 7. doi: 10.1111/febs.12805. PMID:24702737<ref>PMID:24702737</ref>

-	~~Authors: Mine~~, S.~~, Nakamura, T~~., Fukuda, Y., Inoue, T., ~~Uegaki, K~~., Sato, T.	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fukuda, Y.]]
		+	[[Category: Inoue, T.]]
		+	[[Category: Mine, S.]]
		+	[[Category: Nakamura, T.]]
		+	[[Category: Sato, T.]]
		+	[[Category: Uegaki, K.]]
		+	[[Category: Deacetylation]]
		+	[[Category: Hydrolase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Zinc binding]]

OCA at 19:36, 7 August 2013

2013-08-07T19:36:53Z

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	Authors: Mine, S., Nakamura, T., Fukuda, Y., Inoue, T., Uegaki, K., Sato, T.		Authors: Mine, S., Nakamura, T., Fukuda, Y., Inoue, T., Uegaki, K., Sato, T.

	Description: Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii		Description: Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii

OCA: Protected "3we7" [edit=sysop:move=sysop]

2013-07-10T14:41:32Z

Protected "3we7" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:41, 10 July 2013

OCA: New page: '''Unreleased structure''' The entry 3we7 is ON HOLD Authors: Mine, S., Nakamura, T., Fukuda, Y., Inoue, T., Uegaki, K., Sato, T. Description: Crystal Structure of Diacetylchitobiose D...

2013-07-10T14:41:31Z

New page: '''Unreleased structure''' The entry 3we7 is ON HOLD Authors: Mine, S., Nakamura, T., Fukuda, Y., Inoue, T., Uegaki, K., Sato, T. Description: Crystal Structure of Diacetylchitobiose D...

New page

'''Unreleased structure'''

The entry 3we7 is ON HOLD

Authors: Mine, S., Nakamura, T., Fukuda, Y., Inoue, T., Uegaki, K., Sato, T.

Description: Crystal Structure of Diacetylchitobiose Deacetylase from Pyrococcus horikoshii