3wef - Revision history

OCA at 13:07, 8 November 2023

2023-11-08T13:07:02Z

←Older revision		Revision as of 13:07, 8 November 2023
Line 3:		Line 3:
	<StructureSection load='3wef' size='340' side='right'caption='[[3wef]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='3wef' size='340' side='right'caption='[[3wef]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wef]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WEF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wef]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WEF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FPS:S~~-~~[(2E~~,~~6E)-3,7,11-TRIMETHYLDODECA-2,6,10-TRIENYL]+TRIHYDROGEN+THIODIPHOSPHATE'>FPS</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3vj8\|3vj8]], [[3vj9\|3vj9]], [[3vja\|3vja]], [[3vjb\|3vjb]], [[3vjc\|3vjc]], [[3vjd\|3vjd]], [[3weg\|3weg]], [[3weh\|3weh]], [[3wei\|3wei]], [[3wej\|3wej]], [[3wek\|3wek]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FPS:S-[(2E,6E)-3,7,11-TRIMETHYLDODECA-2,6,10-TRIENYL]+TRIHYDROGEN+THIODIPHOSPHATE'>FPS</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>FDFT1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>~~[~~https://en.wikipedia.org/wiki/Squalene_synthase Squalene synthase]~~, ~~with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=~~2~~.5.1.21 2.5.1.21~~] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wef OCA], [https://pdbe.org/3wef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wef RCSB], [https://www.ebi.ac.uk/pdbsum/3wef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wef ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wef OCA], [https://pdbe.org/3wef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wef RCSB], [https://www.ebi.ac.uk/pdbsum/3wef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wef ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FDFT_HUMAN FDFT_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Squalene synthase]]~~	+	[[Category: Jeng WY]]
-	[[Category: Jeng~~, W Y~~]]	+	[[Category: Liu CI]]
-	[[Category: Liu~~, C I~~]]	+	[[Category: Wang AHJ]]
-	[[Category: Wang~~, A H.J]]~~	+
-	~~[[Category: Cholesterol biosynthesis]]~~	+
-	~~[[Category: Farnesyl-diphosphate farnesyltransferase]]~~	+
-	~~[[Category: Head-to-head synthase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:33, 3 August 2022

2022-08-03T05:33:41Z

←Older revision		Revision as of 05:33, 3 August 2022
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	<StructureSection load='3wef' size='340' side='right'caption='[[3wef]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='3wef' size='340' side='right'caption='[[3wef]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wef]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WEF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wef]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WEF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FPS:S-[(2E,6E)-3,7,11-TRIMETHYLDODECA-2,6,10-TRIENYL]+TRIHYDROGEN+THIODIPHOSPHATE'>FPS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FPS:S-[(2E,6E)-3,7,11-TRIMETHYLDODECA-2,6,10-TRIENYL]+TRIHYDROGEN+THIODIPHOSPHATE'>FPS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vj8\|3vj8]], [[3vj9\|3vj9]], [[3vja\|3vja]], [[3vjb\|3vjb]], [[3vjc\|3vjc]], [[3vjd\|3vjd]], [[3weg\|3weg]], [[3weh\|3weh]], [[3wei\|3wei]], [[3wej\|3wej]], [[3wek\|3wek]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3vj8\|3vj8]], [[3vj9\|3vj9]], [[3vja\|3vja]], [[3vjb\|3vjb]], [[3vjc\|3vjc]], [[3vjd\|3vjd]], [[3weg\|3weg]], [[3weh\|3weh]], [[3wei\|3wei]], [[3wej\|3wej]], [[3wek\|3wek]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FDFT1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FDFT1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Squalene_synthase Squalene synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.21 2.5.1.21] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Squalene_synthase Squalene synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.21 2.5.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wef OCA], [~~http~~://pdbe.org/3wef PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wef RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wef PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wef ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wef OCA], [https://pdbe.org/3wef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wef RCSB], [https://www.ebi.ac.uk/pdbsum/3wef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wef ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:56, 25 December 2019

2019-12-25T09:56:06Z

←Older revision		Revision as of 09:56, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate==		==Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate==
-	<StructureSection load='3wef' size='340' side='right' caption='[[3wef]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='3wef' size='340' side='right'caption='[[3wef]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3wef]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3wef]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WEF FirstGlance]. <br>
Line 10:		Line 10:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wef OCA], [http://pdbe.org/3wef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wef RCSB], [http://www.ebi.ac.uk/pdbsum/3wef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wef ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wef OCA], [http://pdbe.org/3wef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wef RCSB], [http://www.ebi.ac.uk/pdbsum/3wef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wef ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Squalene synthase (SQS) is a divalent metal-ion-dependent enzyme that catalyzes the two-step reductive `head-to-head' condensation of two molecules of farnesyl pyrophosphate to form squalene using presqualene diphosphate (PSPP) as an intermediate. In this paper, the structures of human SQS and its mutants in complex with several substrate analogues and intermediates coordinated with Mg2+ or Mn2+ are presented, which stepwise delineate the biosynthetic pathway. Extensive study of the SQS active site has identified several critical residues that are involved in binding reduced nicotinamide dinucleotide phosphate (NADPH). Based on mutagenesis data and a locally closed (JK loop-in) structure observed in the hSQS-(F288L)-PSPP complex, an NADPH-binding model is proposed for SQS. The results identified four major steps (substrate binding, condensation, intermediate formation and translocation) of the ordered sequential mechanisms involved in the `1'-1' isoprenoid biosynthetic pathway. These new findings clarify previous hypotheses based on site-directed mutagenesis and biochemical analysis.
		+
		+	Structural insights into the catalytic mechanism of human squalene synthase.,Liu CI, Jeng WY, Chang WJ, Shih MF, Ko TP, Wang AH Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):231-41. doi:, 10.1107/S1399004713026230. Epub 2014 Jan 17. PMID:24531458<ref>PMID:24531458</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3wef" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Squalene synthase\|Squalene synthase]]		*[[Squalene synthase\|Squalene synthase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Squalene synthase]]		[[Category: Squalene synthase]]
	[[Category: Jeng, W Y]]		[[Category: Jeng, W Y]]

OCA at 18:24, 5 August 2016

2016-08-05T18:24:09Z

←Older revision		Revision as of 18:24, 5 August 2016
Line 1:		Line 1:
-	{{STRUCTURE_3wef\| PDB=3wef \| SCENE= }}
-	===Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate===

-	==~~About this Structure~~==	+	==Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate==
-	[[3wef]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA].	+	<StructureSection load='3wef' size='340' side='right' caption='[[3wef]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3wef]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WEF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FPS:S-[(2E,6E)-3,7,11-TRIMETHYLDODECA-2,6,10-TRIENYL]+TRIHYDROGEN+THIODIPHOSPHATE'>FPS</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vj8\|3vj8]], [[3vj9\|3vj9]], [[3vja\|3vja]], [[3vjb\|3vjb]], [[3vjc\|3vjc]], [[3vjd\|3vjd]], [[3weg\|3weg]], [[3weh\|3weh]], [[3wei\|3wei]], [[3wej\|3wej]], [[3wek\|3wek]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FDFT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Squalene_synthase Squalene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.21 2.5.1.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wef OCA], [http://pdbe.org/3wef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wef RCSB], [http://www.ebi.ac.uk/pdbsum/3wef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wef ProSAT]</span></td></tr>
		+	</table>
		+
		+	==See Also==
		+	*[[Squalene synthase\|Squalene synthase]]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
	[[Category: Squalene synthase]]		[[Category: Squalene synthase]]
-	[[Category: Jeng, W Y.]]	+	[[Category: Jeng, W Y]]
-	[[Category: Liu, C I.]]	+	[[Category: Liu, C I]]
-	[[Category: Wang, A H.J.]]	+	[[Category: Wang, A H.J]]
	[[Category: Cholesterol biosynthesis]]		[[Category: Cholesterol biosynthesis]]
	[[Category: Farnesyl-diphosphate farnesyltransferase]]		[[Category: Farnesyl-diphosphate farnesyltransferase]]

OCA at 05:13, 13 February 2014

2014-02-13T05:13:09Z

←Older revision		Revision as of 05:13, 13 February 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_3wef\| PDB=3wef \| SCENE= }}
		+	===Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate===

-	~~The entry~~ 3wef is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[3wef]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEF OCA].
-	~~Authors~~: ~~Liu, C~~.I., Jeng, W.Y., Wang, A.H.J.	+	[[Category: Squalene synthase]]
-		+	[[Category: Jeng, W Y.]]
-	~~Description~~: ~~Crystal structure of the human squalene~~ synthase ~~in complex with farnesyl thiopyrophosphate~~	+	[[Category: Liu, C I.]]
		+	[[Category: Wang, A H.J.]]
		+	[[Category: Cholesterol biosynthesis]]
		+	[[Category: Farnesyl-diphosphate farnesyltransferase]]
		+	[[Category: Head-to-head synthase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Transferase]]

OCA at 06:53, 14 August 2013

2013-08-14T06:53:11Z

←Older revision		Revision as of 06:53, 14 August 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3wef is ON HOLD	+	The entry 3wef is ON HOLD until Paper Publication

	Authors: Liu, C.I., Jeng, W.Y., Wang, A.H.J.		Authors: Liu, C.I., Jeng, W.Y., Wang, A.H.J.

	Description: Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate		Description: Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate

OCA: Protected "3wef" [edit=sysop:move=sysop]

2013-07-18T04:40:10Z

Protected "3wef" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:40, 18 July 2013

OCA: New page: '''Unreleased structure''' The entry 3wef is ON HOLD Authors: Liu, C.I., Jeng, W.Y., Wang, A.H.J. Description: Crystal structure of the human squalene synthase in complex with farnesyl...

2013-07-18T04:40:10Z

New page: '''Unreleased structure''' The entry 3wef is ON HOLD Authors: Liu, C.I., Jeng, W.Y., Wang, A.H.J. Description: Crystal structure of the human squalene synthase in complex with farnesyl...

New page

'''Unreleased structure'''

The entry 3wef is ON HOLD

Authors: Liu, C.I., Jeng, W.Y., Wang, A.H.J.

Description: Crystal structure of the human squalene synthase in complex with farnesyl thiopyrophosphate